FastQCFastQC Report
Fri 27 May 2016
SRR939827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208795
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55330.25049857501488365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.19209569018401434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27880.12622266892128967No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39600.040.6070061
GTATCAA71200.038.640651
TATCAAC87800.031.7139152
ATCAACG88000.031.428363
TCAACGC88250.031.3930824
CAACGCA89550.030.8850735
AACGCAG93900.029.5054876
TACATGG77850.025.5915722
ACGCAGA107200.025.5792947
CGCAGAG108150.025.3101638
GTACATG76900.025.0435621
ACATGGG79700.023.80883
GCAGAGT112950.022.6385299
AGAGTAC96150.020.99165510-11
GAGTACT63050.020.76374212-13
GTACTTT66650.019.25385514-15
AGTACTT65650.019.00214612-13
CAGAGTA109200.018.87398310-11
TAAGGTG10350.018.79275
TATACCG1300.007620516718.2461955
Produced by FastQC (version 0.11.5)