FastQCFastQC Report
Fri 27 May 2016
SRR939827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208795
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64060.2900223877725185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53590.24262097659583617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37910.17163204371614388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38450.046.462561
GTATCAA68850.038.6131671
ATCAACG86700.030.2195533
TATCAAC88750.029.682832
TCAACGC88500.029.6035754
CAACGCA90050.029.0416935
AACGCAG93500.027.9549056
ACGCAGA108150.024.1681337
CGCAGAG109800.023.7191688
TACATGG76000.022.7569642
GTACATG77750.022.3710421
GCAGAGT118350.021.4485119
ACATGGG78350.021.1105923
AGAGTAC103350.020.642610-11
AGTACTT72150.019.51759312-13
GAGTACT70100.019.41651312-13
GTACTTT70700.018.98591614-15
CAGAGTA113550.018.31134410-11
GTACTAG5200.018.1295431
GAGTACA59950.017.8483031
Produced by FastQC (version 0.11.5)