##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939827_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2208795 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.392385893666003 31.0 31.0 34.0 30.0 34.0 2 31.600343173540324 31.0 31.0 34.0 30.0 34.0 3 31.662176435567808 31.0 31.0 34.0 30.0 34.0 4 35.475492746044786 37.0 35.0 37.0 33.0 37.0 5 35.20024266625015 37.0 35.0 37.0 32.0 37.0 6 35.22157149033749 37.0 35.0 37.0 32.0 37.0 7 35.13647350704796 37.0 35.0 37.0 32.0 37.0 8 35.06989104919198 37.0 35.0 37.0 32.0 37.0 9 36.6813343927345 39.0 35.0 39.0 32.0 39.0 10-11 36.528765684456914 38.0 35.0 39.0 32.0 39.0 12-13 36.47294135490166 38.0 35.0 39.0 32.0 39.0 14-15 37.51105036909265 39.0 36.0 41.0 32.0 41.0 16-17 37.43718724462886 39.0 36.0 41.0 32.0 41.0 18-19 37.400693817217075 39.0 36.0 41.0 32.0 41.0 20-21 37.31339304915123 39.0 36.0 40.5 31.0 41.0 22-23 37.182912402463785 39.0 36.0 40.0 31.0 41.0 24-25 37.017460651622265 39.0 36.0 40.0 31.0 41.0 26-27 36.71355399663618 39.0 35.5 40.0 30.0 41.0 28-29 36.48468373026922 38.0 35.0 40.0 30.0 41.0 30-31 36.225749560280605 38.0 35.0 40.0 30.0 41.0 32-33 36.172499032277784 38.0 35.0 40.0 30.0 41.0 34-35 36.26116366616186 38.0 35.0 40.0 30.0 41.0 36-37 36.23302637863631 38.0 35.0 40.0 30.0 41.0 38-39 36.09046991685512 38.0 35.0 40.0 29.5 41.0 40-41 35.9139195805858 38.0 35.0 40.0 29.0 41.0 42-43 35.70814629696282 38.0 34.5 40.0 28.5 41.0 44-45 35.46097080082126 38.0 34.0 40.0 27.0 41.0 46-47 35.16823064159417 38.0 34.0 40.0 26.5 41.0 48-49 34.85183731401058 38.0 33.5 40.0 26.0 41.0 50-51 34.56375919902028 38.0 33.0 40.0 24.5 41.0 52-53 34.26626418477043 38.0 33.0 40.0 24.0 41.0 54-55 33.935664921371156 37.0 33.0 40.0 23.5 41.0 56-57 32.6207712802682 36.5 31.0 39.5 16.0 41.0 58-59 29.20703754762212 34.0 23.5 38.5 7.0 40.0 60-61 29.201311801230986 34.0 24.0 38.0 8.0 40.0 62-63 31.024247157386718 34.5 29.0 38.0 8.0 40.0 64-65 30.52342363143705 35.0 29.0 38.0 4.0 40.0 66-67 25.02301707492094 28.5 17.5 36.0 2.0 39.0 68-69 17.306314755330394 13.0 7.0 29.0 2.0 35.5 70-71 16.16399507423731 12.5 7.5 25.5 2.0 33.0 72-73 18.255568533974408 17.0 7.0 30.0 2.0 34.0 74-75 18.985110886252457 17.5 7.5 30.5 2.0 35.0 76-77 20.02065537996962 23.0 8.5 31.0 2.0 34.5 78-79 23.128954701545414 28.0 12.0 33.5 2.0 35.5 80-81 25.768528088844825 31.5 21.5 34.5 2.0 36.0 82-83 26.737777611774746 32.5 25.5 35.0 2.0 36.0 84-85 26.73472956974278 33.0 25.0 35.0 2.0 36.0 86-87 26.442924762144067 33.0 25.0 35.0 2.0 35.5 88-89 25.990486441702373 33.0 23.5 35.0 2.0 35.0 90-91 24.36428233493828 31.5 11.0 34.0 2.0 35.0 92-93 22.246664584083177 30.5 2.0 34.0 2.0 35.0 94-95 17.788161644697674 20.0 2.0 33.0 2.0 35.0 96-97 16.459473830753872 13.5 2.0 32.5 2.0 34.5 98-99 15.349847994042001 2.0 2.0 32.0 2.0 34.0 100-101 14.723706138414837 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4579458264846714 1101 2 0.4979601616011706 1101 3 0.4042180466771832 1101 4 0.30428253180947706 1101 5 0.44031573650579503 1101 6 0.550610301613375 1101 7 0.46869774067967995 1101 8 0.41580548152612096 1101 9 0.6661901785129771 1101 10-11 0.5218984694401385 1101 12-13 0.5968927611113628 1101 14-15 0.544631069810741 1101 16-17 0.5796648383875507 1101 18-19 0.5754209180965617 1101 20-21 0.5546605718962425 1101 22-23 0.6348397096604614 1101 24-25 0.573522914596893 1101 26-27 0.5768871852407358 1101 28-29 0.4918991149760501 1101 30-31 0.6011897825232424 1101 32-33 0.3716876325536731 1101 34-35 0.5907215313519245 1101 36-37 0.5613752314619802 1101 38-39 0.6119409026798053 1101 40-41 0.6153639244570925 1101 42-43 0.5905905288072333 1101 44-45 0.7472861278305274 1101 46-47 0.5124135399121812 1101 48-49 0.3621798591841454 1101 50-51 0.4560922488571748 1101 52-53 0.48782453554268557 1101 54-55 0.3149326198699427 1101 56-57 1.0695492102978577 1101 58-59 -8.407621005494796 1101 60-61 -9.521853429370474 1101 62-63 -2.7859164387572584 1101 64-65 -0.3007736523527882 1101 66-67 -1.2368538041593453 1101 68-69 -3.736866724449639 1101 70-71 -4.256512992798074 1101 72-73 -7.204931524483252 1101 74-75 -7.930947911172888 1101 76-77 -8.658654885386845 1101 78-79 -11.789494119567447 1101 80-81 -8.387638249122812 1101 82-83 -4.592764326269329 1101 84-85 -3.6791739380599218 1101 86-87 -3.605396696055095 1101 88-89 -3.5436156183200715 1101 90-91 -8.51439661793305 1101 92-93 -13.965665098843385 1101 94-95 -12.285297878915904 1101 96-97 -12.19417021447098 1101 98-99 -11.840268676396734 1101 100-101 -11.576920997235966 1102 1 0.38501118741903895 1102 2 0.4504024069576502 1102 3 0.42532185925543686 1102 4 0.32659117399652615 1102 5 0.4412127128551191 1102 6 0.35603246361793595 1102 7 0.35733118091841476 1102 8 0.38866980313416377 1102 9 0.6315142390888937 1102 10-11 0.5731560371421338 1102 12-13 0.6750892040770466 1102 14-15 0.8180197709958463 1102 16-17 0.8267586693169378 1102 18-19 0.7826130219605929 1102 20-21 0.8089263547353553 1102 22-23 0.6728874569292245 1102 24-25 0.6990387188742488 1102 26-27 0.8354650575373697 1102 28-29 0.5987301987224285 1102 30-31 0.6613922453493117 1102 32-33 0.4800901399713027 1102 34-35 0.6062025199124719 1102 36-37 0.6809324887381152 1102 38-39 0.7505563347891595 1102 40-41 0.7002559614337898 1102 42-43 0.7190279376946691 1102 44-45 0.7054185133074 1102 46-47 0.784776206487372 1102 48-49 0.6505513240168384 1102 50-51 0.49653336227408573 1102 52-53 0.6742249677377927 1102 54-55 0.45820100960016674 1102 56-57 1.2830325827917264 1102 58-59 -8.767599224233543 1102 60-61 -9.749959402924247 1102 62-63 -2.7071381617064745 1102 64-65 0.18798541602664898 1102 66-67 -1.0877422052089614 1102 68-69 -3.5747836983978782 1102 70-71 -4.0442880455361685 1102 72-73 -6.672236908056409 1102 74-75 -7.403726908548846 1102 76-77 -8.502949738491962 1102 78-79 -11.477519571056977 1102 80-81 -7.772549580523844 1102 82-83 -3.4694034948082724 1102 84-85 -2.5411223319062906 1102 86-87 -2.4466464602343514 1102 88-89 -2.458150751322968 1102 90-91 -7.934295600902505 1102 92-93 -14.012068618146385 1102 94-95 -12.138601084362934 1102 96-97 -12.101344738970612 1102 98-99 -11.582135862154876 1102 100-101 -11.432762491396186 1103 1 0.2346966455688353 1103 2 0.23446911246181656 1103 3 0.23098067879097428 1103 4 0.1409716669533836 1103 5 0.14729567143450595 1103 6 0.15672902398441124 1103 7 0.3050979547374695 1103 8 0.24733175341936686 1103 9 0.3233483051657302 1103 10-11 0.35991657041734726 1103 12-13 0.32844166278426457 1103 14-15 0.38831159709884133 1103 16-17 0.3746497091700789 1103 18-19 0.514421564255052 1103 20-21 0.35569250408091335 1103 22-23 0.429110817267194 1103 24-25 0.3755578868576208 1103 26-27 0.3492707415034886 1103 28-29 0.4506723531978878 1103 30-31 0.49915046932993334 1103 32-33 0.492059913777787 1103 34-35 0.43917388214482145 1103 36-37 0.10044896184594876 1103 38-39 0.07763899580063338 1103 40-41 0.27172716230889193 1103 42-43 0.23413307136971895 1103 44-45 0.32026254162245493 1103 46-47 0.38781454245411595 1103 48-49 0.5336947307921704 1103 50-51 0.6265464384975772 1103 52-53 0.5894386508321645 1103 54-55 0.4898316398154634 1103 56-57 1.06575729894967 1103 58-59 -6.323895090554306 1103 60-61 -8.635981883970011 1103 62-63 -2.580504576767602 1103 64-65 -0.17277627399141338 1103 66-67 -1.7773966911499244 1103 68-69 -4.4879686808581365 1103 70-71 -4.693698986814704 1103 72-73 -7.484342054304747 1103 74-75 -8.14725124415515 1103 76-77 -9.063866708663923 1103 78-79 -12.136516809123876 1103 80-81 -8.457892684701946 1103 82-83 -4.635500451652543 1103 84-85 -3.7630503623839466 1103 86-87 -3.501056332783083 1103 88-89 -3.654828887625836 1103 90-91 -8.894618902146004 1103 92-93 -14.54199221444313 1103 94-95 -12.60644007078797 1103 96-97 -12.303693010771651 1103 98-99 -11.852423287605735 1103 100-101 -11.625272581462866 1104 1 0.11311973672833986 1104 2 0.17630880523286407 1104 3 0.1619294253879815 1104 4 0.14464782311578972 1104 5 0.05718263630336651 1104 6 0.12360906689684725 1104 7 -0.02309391420445195 1104 8 0.0934100754555729 1104 9 0.028762168646444763 1104 10-11 0.16218753589924262 1104 12-13 0.07566179632458869 1104 14-15 0.16313830872139334 1104 16-17 0.19120229845524506 1104 18-19 0.2739519973955993 1104 20-21 0.23851049364153454 1104 22-23 0.2765864275823233 1104 24-25 0.21674373286957405 1104 26-27 0.04770055825672159 1104 28-29 0.015787800100937943 1104 30-31 0.1090219030718842 1104 32-33 0.13165600869144356 1104 34-35 0.1959181200625295 1104 36-37 0.19627396230777094 1104 38-39 0.2166027275404403 1104 40-41 0.27603106335850924 1104 42-43 0.26062373456252175 1104 44-45 0.18047179447945894 1104 46-47 0.0749032156100995 1104 48-49 0.04701951402347504 1104 50-51 -0.11734818656939439 1104 52-53 0.00979185055219034 1104 54-55 -0.1488329295789157 1104 56-57 0.49163362739125915 1104 58-59 -6.429817205031711 1104 60-61 -8.584745927837574 1104 62-63 -2.8106916139554166 1104 64-65 -0.5943495566216583 1104 66-67 -2.015534365353407 1104 68-69 -4.188560539947712 1104 70-71 -4.861243413021667 1104 72-73 -7.7741403959635385 1104 74-75 -8.611912627958032 1104 76-77 -9.199912196392622 1104 78-79 -12.203350942846193 1104 80-81 -8.65571767695349 1104 82-83 -5.0734652308881785 1104 84-85 -4.2732458288001105 1104 86-87 -4.060417736632754 1104 88-89 -4.133077270712366 1104 90-91 -9.520258021702155 1104 92-93 -14.946106321016767 1104 94-95 -12.439335066002057 1104 96-97 -12.30177779019886 1104 98-99 -11.742532183383663 1104 100-101 -11.3179126062032 1105 1 0.17121797603389055 1105 2 0.27843403611445083 1105 3 0.2384543641372936 1105 4 0.12116659061001656 1105 5 0.24564647726894862 1105 6 0.21155829594199105 1105 7 0.23560804680029435 1105 8 0.22629901291088572 1105 9 0.2602614731027586 1105 10-11 0.3085954527154442 1105 12-13 0.36286601337508984 1105 14-15 0.3799076724099635 1105 16-17 0.5370194846538041 1105 18-19 0.44188747279646634 1105 20-21 0.32228940862483313 1105 22-23 0.41168434024374534 1105 24-25 0.37017037138693354 1105 26-27 0.4791155091244832 1105 28-29 0.32076454390305287 1105 30-31 0.4456226932283798 1105 32-33 0.3442847353728453 1105 34-35 0.4688444309530624 1105 36-37 0.41467703802985767 1105 38-39 0.37036416647273285 1105 40-41 0.4157820826965448 1105 42-43 0.5058484802734 1105 44-45 0.3901338966447838 1105 46-47 0.4274707525285564 1105 48-49 0.4756138672071515 1105 50-51 0.33238969231913984 1105 52-53 0.5300847650472917 1105 54-55 0.47290510024986787 1105 56-57 0.7433136238378779 1105 58-59 -6.460879352714954 1105 60-61 -8.778040151660743 1105 62-63 -2.5901361656215975 1105 64-65 0.016148632684931385 1105 66-67 -1.2524174019882572 1105 68-69 -3.625009190978174 1105 70-71 -4.652255530300669 1105 72-73 -6.056660944867197 1105 74-75 -6.6559943550289695 1105 76-77 -8.621064389369733 1105 78-79 -11.960524437266319 1105 80-81 -8.095003729563246 1105 82-83 -4.133406788709497 1105 84-85 -3.156397437669895 1105 86-87 -2.9706073042243055 1105 88-89 -3.010853893290591 1105 90-91 -8.362511308092023 1105 92-93 -11.608983234351513 1105 94-95 -11.582326780404234 1105 96-97 -11.94175787316097 1105 98-99 -11.491449560498054 1105 100-101 -11.091837815308512 1106 1 0.13233087481004802 1106 2 0.07337160288842526 1106 3 0.18545438896163802 1106 4 0.16752605367350526 1106 5 0.19241839915563475 1106 6 0.07978318201933376 1106 7 0.13968233060624868 1106 8 0.20515054506473263 1106 9 0.18237509272167074 1106 10-11 0.19806798692034278 1106 12-13 0.20939980890961607 1106 14-15 0.21619908398307075 1106 16-17 0.09041419032373454 1106 18-19 0.12015483660566417 1106 20-21 0.20303841061593175 1106 22-23 0.20116324446907896 1106 24-25 0.34958642318269284 1106 26-27 0.2462929015472497 1106 28-29 0.2271079132972602 1106 30-31 0.23481728845339234 1106 32-33 0.3996598850800339 1106 34-35 0.33419168371781893 1106 36-37 0.31502966081551875 1106 38-39 0.33805762422755237 1106 40-41 0.3488837533749205 1106 42-43 0.5073289245448578 1106 44-45 0.5399236391320272 1106 46-47 0.23225480181002212 1106 48-49 0.21660028829079891 1106 50-51 0.4605703114382891 1106 52-53 0.1723684220572892 1106 54-55 0.04660834441696693 1106 56-57 0.9558740629196407 1106 58-59 -6.526732132175013 1106 60-61 -8.68731946815755 1106 62-63 -2.3308834767469726 1106 64-65 -0.0023452696598482703 1106 66-67 -1.538100055064298 1106 68-69 -3.9141280251145503 1106 70-71 -4.5848694962894445 1106 72-73 -6.0601242921911584 1106 74-75 -6.686385558592809 1106 76-77 -8.443302528836647 1106 78-79 -11.93637690759303 1106 80-81 -8.03422869948129 1106 82-83 -4.158534879984398 1106 84-85 -3.051595027741282 1106 86-87 -2.72932451325606 1106 88-89 -2.93022863831413 1106 90-91 -8.322618938018191 1106 92-93 -11.499799796029937 1106 94-95 -11.600397849223405 1106 96-97 -12.07734399232438 1106 98-99 -11.537295089376027 1106 100-101 -11.158813076678413 1107 1 0.07582119039816604 1107 2 0.11179338930692495 1107 3 0.04842143356007611 1107 4 0.0319368637387214 1107 5 0.026843955791996166 1107 6 0.16777121617543855 1107 7 0.1648853653634248 1107 8 0.006937279664285256 1107 9 0.05275493546641741 1107 10-11 0.07347155397174987 1107 12-13 0.09303623959761609 1107 14-15 0.15165278171332375 1107 16-17 0.13906530292594965 1107 18-19 0.060012390188305176 1107 20-21 0.016952152514058128 1107 22-23 0.09191756582746535 1107 24-25 0.04873206556852239 1107 26-27 0.30408375120167364 1107 28-29 0.28181797961612176 1107 30-31 0.22278642841555296 1107 32-33 0.2945624279176613 1107 34-35 0.04492528611979196 1107 36-37 -0.00571005765380761 1107 38-39 0.21594365847273878 1107 40-41 -0.12590842246513034 1107 42-43 0.04345359365699153 1107 44-45 0.03702042290437646 1107 46-47 0.0934230664609359 1107 48-49 0.19359259603100298 1107 50-51 0.22646178931866956 1107 52-53 0.10438680091149877 1107 54-55 0.23373078980939255 1107 56-57 0.6721784591603424 1107 58-59 -6.73853856079878 1107 60-61 -9.18143796181375 1107 62-63 -2.876009588906168 1107 64-65 -0.319615425023283 1107 66-67 -1.7756581445332174 1107 68-69 -4.134577769192813 1107 70-71 -4.82198815805368 1107 72-73 -6.087378268952017 1107 74-75 -6.8600884566125355 1107 76-77 -9.17434727000056 1107 78-79 -12.476679013541325 1107 80-81 -8.519790685608307 1107 82-83 -4.480629640234074 1107 84-85 -3.6062058336876106 1107 86-87 -3.3886463912958327 1107 88-89 -3.4115825354272253 1107 90-91 -8.434654204306591 1107 92-93 -11.573420699474141 1107 94-95 -12.115718789498116 1107 96-97 -12.451554878349524 1107 98-99 -11.597976702810875 1107 100-101 -11.102448870375847 1108 1 0.02709640758908094 1108 2 0.011509424748414432 1108 3 -0.01665532485291621 1108 4 0.022858755727256153 1108 5 -0.1282486797752469 1108 6 -0.06065558949772054 1108 7 -0.015577017404332594 1108 8 -0.10767063050023751 1108 9 0.007903185468236984 1108 10-11 7.536628323592254E-4 1108 12-13 -0.08201261473696064 1108 14-15 0.033604283343422026 1108 16-17 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2311 60-61 4.21332433341388 2311 62-63 1.6520905245706636 2311 64-65 1.5580694952509901 2311 66-67 -0.4518620271164231 2311 68-69 -3.3007553261660547 2311 70-71 -0.5126013137504959 2311 72-73 -0.9578090493527647 2311 74-75 1.3497472177622605 2311 76-77 4.41053187844085 2311 78-79 5.146628671150474 2311 80-81 2.794453830312726 2311 82-83 1.4961939223292298 2311 84-85 1.1878057881679744 2311 86-87 1.162382643034082 2311 88-89 1.4347972489414076 2311 90-91 2.9381958920344786 2311 92-93 5.0576336196267775 2311 94-95 8.907177129143818 2311 96-97 9.60076885811005 2311 98-99 10.03580918392046 2311 100-101 9.331683793435706 2312 1 -0.35070354069978293 2312 2 -0.3137705531592907 2312 3 -0.2701336434016497 2312 4 -0.19531994280357878 2312 5 -0.2702170737112368 2312 6 -0.25131198252402953 2312 7 -0.31212414831608726 2312 8 -0.3035864658855445 2312 9 -0.39484434099311017 2312 10-11 -0.25756783695307917 2312 12-13 -0.4045080426330898 2312 14-15 -0.5826313894902313 2312 16-17 -0.4476059640388286 2312 18-19 -0.3481455366500441 2312 20-21 -0.34230288100786055 2312 22-23 -0.46317308406663216 2312 24-25 -0.4058800821976618 2312 26-27 -0.4894126176091049 2312 28-29 -0.490956112868389 2312 30-31 -0.417907911969742 2312 32-33 -0.2451620453596206 2312 34-35 -0.35115142318014136 2312 36-37 -0.5466097004328816 2312 38-39 -0.5129262383063988 2312 40-41 -0.500728211691488 2312 42-43 -0.5216287145063561 2312 44-45 -0.4992234310074366 2312 46-47 -0.4764171885898989 2312 48-49 -0.4368528290409728 2312 50-51 -0.5574320275344391 2312 52-53 -0.46446891250978695 2312 54-55 -0.45300229533866343 2312 56-57 0.7988747352136585 2312 58-59 4.222463042729871 2312 60-61 4.053345122992106 2312 62-63 1.490293945419129 2312 64-65 1.1703559787829114 2312 66-67 -0.28521168077083203 2312 68-69 -1.012162870346362 2312 70-71 -0.08325362115460422 2312 72-73 6.761087065963238 2312 74-75 5.367124265976205 2312 76-77 5.431732712381965 2312 78-79 5.412600916343049 2312 80-81 2.931746757369403 2312 82-83 1.7339398447324896 2312 84-85 1.3622286464860842 2312 86-87 1.0170411231220342 2312 88-89 1.2941757951315722 2312 90-91 2.6056574511283976 2312 92-93 4.7161806809373665 2312 94-95 8.760512189483705 2312 96-97 9.15844847525169 2312 98-99 9.540211495008922 2312 100-101 9.030277647672206 2313 1 -0.31698036543726715 2313 2 -0.2930192000549212 2313 3 -0.2658322754261562 2313 4 -0.3418173306946173 2313 5 -0.4045518975178126 2313 6 -0.337954855390187 2313 7 -0.4672148621805192 2313 8 -0.37785505017122034 2313 9 -0.4984967565847711 2313 10-11 -0.5234676744629283 2313 12-13 -0.44947790549947086 2313 14-15 -0.557472722144702 2313 16-17 -0.4579270291138684 2313 18-19 -0.6442179632312133 2313 20-21 -0.4876757765755926 2313 22-23 -0.5718263986472252 2313 24-25 -0.46927216067687993 2313 26-27 -0.5911711911909379 2313 28-29 -0.5775247981432869 2313 30-31 -0.4930404498238943 2313 32-33 -0.4797217004167251 2313 34-35 -0.6794938556257506 2313 36-37 -0.6778311659360696 2313 38-39 -0.8655328021018462 2313 40-41 -1.0172281873263742 2313 42-43 -0.6815012933275213 2313 44-45 -0.7113843147387655 2313 46-47 -0.709923262226333 2313 48-49 -0.8262200584579134 2313 50-51 -0.42766020268773985 2313 52-53 -0.5069816789928012 2313 54-55 -0.5287984046489953 2313 56-57 0.5508080655223537 2313 58-59 4.103817676219641 2313 60-61 3.7022381903591395 2313 62-63 1.2595341569419851 2313 64-65 1.1106041333350731 2313 66-67 1.8038687123645936 2313 68-69 0.20965382665067622 2313 70-71 2.9276226325988848 2313 72-73 8.42588971688836 2313 74-75 9.931407351709503 2313 76-77 8.67406878973198 2313 78-79 6.411057259928217 2313 80-81 3.4470206633334826 2313 82-83 1.9148202825653051 2313 84-85 1.4315380045407728 2313 86-87 1.348810887855791 2313 88-89 1.4109884004687103 2313 90-91 2.8459640819699317 2313 92-93 4.868203910139215 2313 94-95 8.710869684614877 2313 96-97 9.388627617700267 2313 98-99 9.73437100757208 2313 100-101 9.027182941288713 2314 1 -0.34117816864046446 2314 2 -0.3175267167054052 2314 3 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1.3494546615648098 2314 64-65 1.2883180651879513 2314 66-67 1.7771882881859362 2314 68-69 -0.4130379356154492 2314 70-71 2.593505104139302 2314 72-73 8.301237339735994 2314 74-75 9.877256717118847 2314 76-77 8.82123206530748 2314 78-79 6.4807256733922785 2314 80-81 3.3955791742691765 2314 82-83 2.013255450290046 2314 84-85 1.604537616551827 2314 86-87 1.6084758788846045 2314 88-89 1.7845299262987773 2314 90-91 2.777326719481522 2314 92-93 4.917000297267471 2314 94-95 8.826020907118917 2314 96-97 9.589955590347893 2314 98-99 9.987454841987208 2314 100-101 9.19346704068843 2315 1 -0.6829018239655689 2315 2 -0.5904103050159542 2315 3 -0.5651334594938966 2315 4 -0.38544466796862764 2315 5 -0.6898895607903768 2315 6 -0.7280231905765433 2315 7 -0.8018253142912286 2315 8 -0.8117042968311168 2315 9 -1.1021569416736767 2315 10-11 -0.949751525442629 2315 12-13 -0.7623949354384436 2315 14-15 -0.9432145841785484 2315 16-17 -1.0072142155332173 2315 18-19 -1.0196594223994069 2315 20-21 -1.1153428618386059 2315 22-23 -1.0128776353727815 2315 24-25 -0.8956413306563249 2315 26-27 -0.9290688243885512 2315 28-29 -1.1622121706647022 2315 30-31 -1.0473190643499848 2315 32-33 -0.85318695825341 2315 34-35 -1.0023886759625995 2315 36-37 -1.0988694981629195 2315 38-39 -0.9374900466181018 2315 40-41 -0.8618540235192214 2315 42-43 -1.0282003812140843 2315 44-45 -1.096669720123984 2315 46-47 -1.0621240924868545 2315 48-49 -0.8681916738931861 2315 50-51 -0.8594687255225679 2315 52-53 -0.8059997106301466 2315 54-55 -0.842901635306248 2315 56-57 0.3551174748749162 2315 58-59 3.658032301495929 2315 60-61 3.400593666639125 2315 62-63 1.0103690833410148 2315 64-65 0.8572669606467542 2315 66-67 1.8300699105632923 2315 68-69 5.710528346398025 2315 70-71 4.43095780562315 2315 72-73 8.550868187172018 2315 74-75 9.716660229226079 2315 76-77 8.57959106199024 2315 78-79 5.990866252017543 2315 80-81 3.2039862931076755 2315 82-83 1.4975562495895112 2315 84-85 1.1170244428191403 2315 86-87 1.116165972201621 2315 88-89 1.1458724066223418 2315 90-91 2.4378603767257516 2315 92-93 4.332348600497902 2315 94-95 8.220667771869554 2315 96-97 8.93851230373968 2315 98-99 9.197780888045493 2315 100-101 8.528059327389178 2316 1 -0.44801439049215475 2316 2 -0.36764955775557695 2316 3 -0.6504322901153934 2316 4 -0.4014529844851964 2316 5 -0.5208294320972513 2316 6 -0.4692993012209641 2316 7 -0.5311475606909752 2316 8 -0.5952287028096634 2316 9 -0.6616745521194218 2316 10-11 -0.6537407792635648 2316 12-13 -0.5154394887162184 2316 14-15 -0.7450007622197745 2316 16-17 -0.8205493767550465 2316 18-19 -0.9000576877106568 2316 20-21 -0.7692641434065877 2316 22-23 -0.9262998469636656 2316 24-25 -2.2171299653082386 2316 26-27 -1.417882598092575 2316 28-29 -0.9291672939334035 2316 30-31 -0.7409878942060999 2316 32-33 -0.6956636832338532 2316 34-35 -0.7059147518639719 2316 36-37 -0.6852396745447606 2316 38-39 -0.9559994862894072 2316 40-41 -0.7328150622262726 2316 42-43 -0.9877874025287312 2316 44-45 -0.8806520384996617 2316 46-47 -0.6678554067932154 2316 48-49 -0.8949050795935989 2316 50-51 -0.7773261994897283 2316 52-53 -1.0962270326362145 2316 54-55 -0.9462935532182541 2316 56-57 0.3769924296243303 2316 58-59 3.8429073327834757 2316 60-61 3.713710308680078 2316 62-63 1.3179382154193142 2316 64-65 1.0658349623302144 2316 66-67 6.197023792673136 2316 68-69 8.743686845581212 2316 70-71 5.380010209042654 2316 72-73 9.31055698351308 2316 74-75 10.296073804231849 2316 76-77 8.706547227080165 2316 78-79 6.344497045996267 2316 80-81 3.363087062231255 2316 82-83 1.7960114205299966 2316 84-85 1.3717778461801622 2316 86-87 1.3538972579237232 2316 88-89 1.4386571740677248 2316 90-91 2.894036561858506 2316 92-93 4.934122310534228 2316 94-95 8.664557612626993 2316 96-97 9.160654352114411 2316 98-99 9.491528869987011 2316 100-101 8.598830675650644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 18.0 4 44.0 5 106.0 6 262.0 7 598.0 8 1429.0 9 2361.0 10 3925.0 11 5918.0 12 8140.0 13 10997.0 14 14703.0 15 20105.0 16 26444.0 17 33960.0 18 37983.0 19 39427.0 20 41265.0 21 40987.0 22 38993.0 23 37908.0 24 39169.0 25 42163.0 26 55026.0 27 79867.0 28 111071.0 29 143734.0 30 169171.0 31 180307.0 32 186880.0 33 192444.0 34 193413.0 35 183332.0 36 150810.0 37 88246.0 38 26458.0 39 1126.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.328311628562 19.26445436337677 14.477887759487073 23.92934624857416 2 15.985841393910965 20.67531371508567 38.74234162119481 24.596503269808558 3 19.524423752824585 24.053042221981762 29.94642625109169 26.47610777410196 4 13.371317845250463 16.735822020603994 36.27923822717817 33.613621906967374 5 13.580683156654889 37.5596629518891 34.37437709522515 14.485276796230858 6 32.823115238451386 36.299627760669395 16.721342053627456 14.155914947251757 7 28.690575630604016 32.48558603220308 20.367213797568358 18.456624539624546 8 26.722561548432193 35.269552682488005 19.24402919427795 18.76385657480185 9 26.310771257631423 16.19249409746038 19.604580778207122 37.89215386670107 10-11 25.232740023406425 26.337211013244776 27.223961481260144 21.206087482088652 12-13 26.008547647020208 23.395131734724135 27.67108310187229 22.92523751638337 14-15 22.578782369808273 24.96692194212172 25.712918450165372 26.741377237904633 16-17 22.389832465212933 28.26183507296965 26.490348810097814 22.8579836517196 18-19 22.473886407485455 26.755625941844833 28.596610728129107 22.173876922540597 20-21 23.04453333152239 25.87241006974391 28.806453292406044 22.276603306327658 22-23 22.707924456547573 25.91161696762262 28.681588830108723 22.698869745721083 24-25 22.63125369262426 26.176897358061744 28.659495335692085 22.532353613621908 26-27 22.301534051191783 26.455863657601075 28.55889101373058 22.683711277476558 28-29 22.308057463180024 26.81132140820603 28.416095322542784 22.464525806071162 30-31 22.510173828945653 26.363619075906637 28.36241198075892 22.76379511438879 32-33 22.259042281067913 26.390353994721096 28.33035281760602 23.020250906604975 34-35 22.793276917536094 26.513251555101608 28.2562058621994 22.437265665162894 36-37 22.879327724699333 26.073423352844905 28.31600145272617 22.73124746972959 38-39 22.433069222370804 26.41646994573627 28.28900565098137 22.861455180911555 40-41 22.781466220521327 26.235333968225504 28.339784145719076 22.643415665534093 42-43 22.510134804676323 26.254917961518537 28.199188309923954 23.035758923881186 44-45 22.483020807506506 26.450542122775495 28.34229418071363 22.724142889004366 46-47 22.816660307741774 26.609575974811868 27.808004999586768 22.765758717859597 48-49 22.551961437856402 26.703464760055358 27.744844613835127 22.99972918825311 50-51 22.534199709366433 26.903484568752408 27.746878904341344 22.815436817539812 52-53 22.89015569005444 26.59054539065606 27.62656213066517 22.89273678862433 54-55 22.446163435125683 26.749375641423583 27.77929844079591 23.02516248265482 56-57 22.524389467023862 26.69008980456461 27.84907265276105 22.93644807565048 58-59 20.943958656387334 26.45730714503066 29.420804381079858 23.177929817502147 60-61 22.254698442700636 26.593369613247873 28.119946247717365 23.031985696334118 62-63 22.20977446213113 26.69259794914538 27.95802129359266 23.139606295130832 64-65 21.481893564418158 26.7435302097346 28.33713502512018 23.43744120072706 66-67 21.637300797213026 26.416330523625316 28.82330989804905 23.123058781112608 68-69 22.38801558517607 25.556812090916477 29.399762186653916 22.655410137253536 70-71 21.452674237949125 25.98495804808314 29.39691920529311 23.16544850867463 72-73 21.299510400895457 26.123848295561636 29.333175847120874 23.243465456422037 74-75 21.830729043288514 26.319880811815416 28.597091775178114 23.25229836971796 76-77 20.41139979504875 26.537638042175267 29.18490210784758 23.8660600549284 78-79 21.46233503461289 26.757300535391543 27.945255930796947 23.83510849919862 80-81 22.28201866180126 26.723885023021655 27.15337356652285 23.84072274865424 82-83 22.336985937319966 26.726471671474357 27.03003160781052 23.906510783395156 84-85 22.247842035065286 26.777417903163446 26.914098824969646 24.060641236801622 86-87 22.420469630319154 26.749659077943928 26.82099011371313 24.008881178023795 88-89 22.33840170771846 26.676128839480352 26.64475426646656 24.34071518633463 90-91 21.933168159583253 26.689105792879374 26.991999963698078 24.38572608383929 92-93 21.77829168961771 26.53761019051198 27.221299897114328 24.46279822275599 94-95 20.405539326571976 26.35001074499635 28.571874163593204 24.672575764838474 96-97 20.176548150016625 26.111492025637205 28.86313448066317 24.848825343682996 98-99 20.58701386135172 26.305261660627167 28.03057848858349 25.07714598943762 100-101 20.49505641386518 26.24859104556182 28.050688947182877 25.205663593390128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 251.0 1 191.5 2 144.0 3 170.0 4 192.0 5 214.0 6 287.5 7 459.0 8 688.0 9 899.5 10 1036.0 11 1181.0 12 1353.0 13 1532.0 14 1791.5 15 2000.5 16 2208.5 17 2459.5 18 2723.0 19 3098.0 20 3662.0 21 4307.0 22 5107.5 23 6257.0 24 7587.5 25 9328.5 26 11648.5 27 14538.0 28 17565.5 29 20782.5 30 24444.5 31 28452.0 32 33057.5 33 37145.5 34 40292.0 35 43508.5 36 47500.5 37 51307.5 38 54919.0 39 58639.0 40 62154.5 41 66608.0 42 70594.0 43 73571.5 44 77161.5 45 80642.5 46 83615.5 47 86150.5 48 87849.0 49 88702.5 50 88203.0 51 87123.5 52 86050.0 53 84254.5 54 81668.0 55 76819.0 56 70254.0 57 62780.0 58 54265.0 59 45712.0 60 37324.5 61 29773.5 62 23067.0 63 17057.0 64 12464.0 65 9175.0 66 6698.5 67 4920.5 68 3482.5 69 2453.0 70 1720.0 71 1133.0 72 790.0 73 607.5 74 436.0 75 286.0 76 172.0 77 111.0 78 72.0 79 34.0 80 17.5 81 9.0 82 3.0 83 2.5 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020282552251340662 2 0.004663176075643054 3 0.0028975074644772376 4 0.0 5 0.0631566080147773 6 4.527355413245684E-5 7 0.0 8 2.7164132479474105E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.0373099359605575E-4 16-17 0.0 18-19 0.0014261169551723905 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.3582066239737053E-4 28-29 0.0018788524964969586 30-31 0.008466154622769428 32-33 2.2636777066228417E-4 34-35 7.470136431855379E-4 36-37 0.024130804352599493 38-39 0.022908418391023158 40-41 0.02471936055632143 42-43 0.0254890109765732 44-45 0.02614547751149382 46-47 0.027730051906129816 48-49 0.02802433000799078 50-51 0.02336115393234773 52-53 0.01960344893935381 54-55 0.014872362532512073 56-57 0.14865571499392202 58-59 0.7616822747244538 60-61 0.30976165737426964 62-63 3.848252101258831E-4 64-65 0.14478482611559698 66-67 1.4271356101403707 68-69 2.7377597287208637 70-71 2.2049579069130454 72-73 1.8747552398479714 74-75 1.573437100319405 76-77 1.1255458292870093 78-79 0.42874055763436625 80-81 2.2636777066228417E-4 82-83 2.490045477285126E-4 84-85 4.527355413245684E-5 86-87 0.00357661077646409 88-89 0.0 90-91 0.22885781613956932 92-93 0.17749496897629702 94-95 1.299826375919902 96-97 1.2844107307378005 98-99 1.012135576185205 100-101 0.4086164628224892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2208795.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.36428585527542 #Duplication Level Percentage of deduplicated Percentage of total 1 72.65819334423482 38.04694406005381 2 14.379665387937393 15.059618177543271 3 5.424058937017549 8.520809180215448 4 2.5608292936035135 5.363839886272697 5 1.4280189769594227 3.7388596958130584 6 0.833699267358993 2.6193640051991998 7 0.554387719026693 2.0321081895637505 8 0.38387415033714833 1.6081036592564324 9 0.2840617915052184 1.338722356584681 >10 1.374900223641292 12.66724914440741 >50 0.07707151355998006 2.7457061390281914 >100 0.036833543540575886 3.5221018363036998 >500 0.002850844944180191 1.074788471911066 >1k 0.0013822278517236412 1.1295706108682229 >5k 1.7277848146545516E-4 0.5322145869789604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6406 0.2900223877725185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5359 0.24262097659583617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3791 0.17163204371614388 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.054710826491368E-5 0.0 0.0 0.0 0.0 3 9.054710826491368E-5 0.0 0.0 0.0 0.0 4 9.054710826491368E-5 0.0 0.0 0.0 0.0 5 9.054710826491368E-5 0.0 0.0 0.0 0.0 6 2.2636777066228417E-4 0.0 0.0 0.0 0.0 7 3.1691487892719785E-4 0.0 0.0 0.0 0.0 8 3.1691487892719785E-4 0.0 0.0 0.0 0.0 9 3.1691487892719785E-4 0.0 0.0 0.0 0.0 10-11 3.1691487892719785E-4 0.0 0.0 0.0 4.527355413245684E-5 12-13 3.1691487892719785E-4 0.0 0.0 0.0 3.8482521012588315E-4 14-15 3.1691487892719785E-4 0.0 0.0 0.0 4.0746198719211155E-4 16-17 4.527355413245684E-4 0.0 0.0 0.0 4.0746198719211155E-4 18-19 4.980090954570252E-4 0.0 0.0 0.0 4.7537231839079675E-4 20-21 4.980090954570252E-4 0.0 0.0 6.791033119868525E-5 5.885562037219389E-4 22-23 4.980090954570252E-4 0.0 0.0 4.3009876425833995E-4 6.338297578543957E-4 24-25 4.980090954570252E-4 0.0 0.0 8.375607514504516E-4 6.338297578543957E-4 26-27 4.980090954570252E-4 0.0 0.0 0.001788305388232045 8.149239743842231E-4 28-29 6.111929807881672E-4 0.0 0.0 0.006111929807881673 8.375607514504516E-4 30-31 7.243768661193094E-4 0.0 0.0 0.022976328722221846 8.828343055829082E-4 32-33 7.470136431855377E-4 0.0 0.0 0.06084765675402199 9.054710826491367E-4 34-35 7.696504202517662E-4 0.0 0.0 0.11739432586546059 9.507446367815936E-4 36-37 8.601975285166799E-4 0.0 0.0 0.21568321188702438 9.507446367815936E-4 38-39 9.507446367815936E-4 0.0 0.0 0.36746280211608595 9.960181909140504E-4 40-41 9.507446367815936E-4 0.0 0.0 0.5368085313485408 9.960181909140504E-4 42-43 9.960181909140504E-4 0.0 0.0 0.7085990325041482 9.960181909140504E-4 44-45 0.001086565299178964 0.0 0.0 0.9112660975780913 0.0010639285221127357 46-47 0.0011771124074438778 0.0 0.0 1.1426818695261445 0.001131838853311421 48-49 0.0012223859615763347 0.0 0.0 1.3676914335644548 0.001131838853311421 50-51 0.0013129330698412483 4.527355413245684E-5 0.0 1.603272372492694 0.0011771124074438778 52-53 0.0013129330698412483 4.527355413245684E-5 0.0 1.8251580612958649 0.0011771124074438778 54-55 0.0013129330698412483 4.527355413245684E-5 0.0 2.0607842737782365 0.0011997491845101064 56-57 0.0013129330698412483 4.527355413245684E-5 0.0 2.267163770291041 0.0012223859615763347 58-59 0.0013129330698412483 4.527355413245684E-5 0.0 2.4044558231977167 0.0012223859615763347 60-61 0.0013129330698412483 9.054710826491368E-5 0.0 2.513338720886275 0.0012902962927750197 62-63 0.001403480178106162 9.054710826491368E-5 0.0 2.6043385646925135 0.0013129330698412483 64-65 0.001403480178106162 9.054710826491368E-5 0.0 2.701133423427706 0.0013129330698412483 66-67 0.001403480178106162 9.054710826491368E-5 0.0 2.7782342861152802 0.0013129330698412483 68-69 0.0014487537322386188 9.054710826491368E-5 0.0 2.867740102635147 0.0013129330698412483 70-71 0.0014487537322386188 9.054710826491368E-5 0.0 3.03289802811035 0.0013129330698412483 72-73 0.0014487537322386188 9.054710826491368E-5 0.0 3.239164340737823 0.001358206623973705 74-75 0.0014487537322386188 9.054710826491368E-5 0.0 3.4777333342388044 0.001403480178106162 76-77 0.0014487537322386188 9.054710826491368E-5 0.0 3.825547413861404 0.001403480178106162 78-79 0.0014713905093048474 9.054710826491368E-5 0.0 4.319662983663038 0.0014940272863710757 80-81 0.0016072111717022179 9.054710826491368E-5 0.0 4.865684683277534 0.0014940272863710757 82-83 0.0016298479487684462 9.054710826491368E-5 0.0 5.3795621594579845 0.0017430318340995884 84-85 0.0016977582799671312 9.054710826491368E-5 0.0 5.8003798451191715 0.0017656686111658167 86-87 0.0017203950570333598 9.054710826491368E-5 0.0 6.168793391872039 0.0018109421652982736 88-89 0.0017203950570333598 9.054710826491368E-5 0.0 6.554365615641108 0.0018562157194307303 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3845 0.0 46.46256 1 GTATCAA 6885 0.0 38.613167 1 ATCAACG 8670 0.0 30.219553 3 TATCAAC 8875 0.0 29.68283 2 TCAACGC 8850 0.0 29.603575 4 CAACGCA 9005 0.0 29.041693 5 AACGCAG 9350 0.0 27.954905 6 ACGCAGA 10815 0.0 24.168133 7 CGCAGAG 10980 0.0 23.719168 8 TACATGG 7600 0.0 22.756964 2 GTACATG 7775 0.0 22.371042 1 GCAGAGT 11835 0.0 21.448511 9 ACATGGG 7835 0.0 21.110592 3 AGAGTAC 10335 0.0 20.6426 10-11 AGTACTT 7215 0.0 19.517593 12-13 GAGTACT 7010 0.0 19.416513 12-13 GTACTTT 7070 0.0 18.985916 14-15 CAGAGTA 11355 0.0 18.311344 10-11 GTACTAG 520 0.0 18.129543 1 GAGTACA 5995 0.0 17.848303 1 >>END_MODULE