FastQCFastQC Report
Fri 27 May 2016
SRR939826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345019
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59800.25500859481309107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45240.19291954564120803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30750.13112900151342055No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23640.10080941774885405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43450.044.5004841
GTATCAA75800.038.5420761
TATCAAC91250.031.6045512
ATCAACG92950.030.8763
TCAACGC93350.030.7436984
CAACGCA96150.029.7511775
AACGCAG99400.028.7784256
TACATGG80700.026.2920842
GTACATG82100.026.2443031
ACGCAGA109950.025.5463547
CGCAGAG110150.025.07238
ACATGGG84600.023.9688643
GCAGAGT115350.022.1474029
GAGTACT63800.020.79437312-13
AGAGTAC98350.020.28169410-11
GTGTACG2151.1841839E-619.6932221
GAGTACA59650.019.4015941
AGTACTT67600.019.34708212-13
GTACTTT69450.019.24267814-15
TAAGGTG11750.019.2199925
Produced by FastQC (version 0.11.5)