FastQCFastQC Report
Fri 27 May 2016
SRR939826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345019
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67210.28660748590949586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56580.24127736278469386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38570.16447627929667094No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42350.048.5228161
GTATCAA75300.039.4539831
TATCAAC93200.031.4603562
ATCAACG94350.030.7653373
TCAACGC95400.030.3770074
CAACGCA98300.029.4808395
AACGCAG102250.028.341976
ACGCAGA116850.024.6383787
CGCAGAG117450.024.3105968
GTACATG83050.023.7148271
TACATGG82050.023.0720062
GCAGAGT128400.021.6094139
ACATGGG84950.021.2722643
GAGTACT72500.020.64062112-13
TAAGGTG11450.019.8832745
AGAGTAC110050.019.58847410-11
GTACTTT77000.019.24955414-15
CAGAGTA121300.019.1598510-11
GTATAGG5400.018.4559761
TGGTATC22600.018.2642522
Produced by FastQC (version 0.11.5)