##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939826_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2345019 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.360626928822324 31.0 31.0 34.0 30.0 34.0 2 31.586921897008082 31.0 31.0 34.0 30.0 34.0 3 31.631570575760794 31.0 31.0 34.0 30.0 34.0 4 35.45240187819374 37.0 35.0 37.0 33.0 37.0 5 35.17503525557789 37.0 35.0 37.0 32.0 37.0 6 35.19817621946773 37.0 35.0 37.0 32.0 37.0 7 35.09744995669545 37.0 35.0 37.0 32.0 37.0 8 35.054164166686924 37.0 35.0 37.0 32.0 37.0 9 36.64197347654753 39.0 35.0 39.0 32.0 39.0 10-11 36.49855502236869 38.0 35.0 39.0 32.0 39.0 12-13 36.42062303119933 38.0 35.0 39.0 32.0 39.0 14-15 37.447137102087446 39.0 36.0 41.0 32.0 41.0 16-17 37.36029473535182 39.0 36.0 41.0 32.0 41.0 18-19 37.32901801648515 39.0 36.0 41.0 32.0 41.0 20-21 37.235639028937506 39.0 36.0 40.0 31.0 41.0 22-23 37.11446623673412 39.0 36.0 40.0 31.0 41.0 24-25 36.957943624337375 39.0 36.0 40.0 31.0 41.0 26-27 36.65350366031149 38.5 35.0 40.0 30.0 41.0 28-29 36.42489719699499 38.0 35.0 40.0 30.0 41.0 30-31 36.14533421690827 38.0 35.0 40.0 29.5 41.0 32-33 36.12242224903082 38.0 35.0 40.0 30.0 41.0 34-35 35.975584632789754 38.0 35.0 40.0 29.0 41.0 36-37 36.03133492735027 38.0 35.0 40.0 29.5 41.0 38-39 35.93125428834479 38.0 35.0 40.0 29.0 41.0 40-41 35.8408620569812 38.0 35.0 40.0 29.0 41.0 42-43 35.6132408735281 38.0 34.0 40.0 27.5 41.0 44-45 35.3653290229205 38.0 34.0 40.0 27.0 41.0 46-47 35.09412183867167 38.0 34.0 40.0 26.5 41.0 48-49 34.78019367860132 38.0 33.0 40.0 26.0 41.0 50-51 34.53416027759263 38.0 33.0 40.0 24.5 41.0 52-53 34.272071143133594 37.5 33.0 40.0 24.0 41.0 54-55 33.99377851522738 37.0 33.0 40.0 24.0 41.0 56-57 33.61200762126021 37.0 32.0 40.0 23.0 41.0 58-59 33.219170292436864 36.0 31.5 39.0 22.0 41.0 60-61 32.88797617418025 36.0 31.0 39.0 22.0 40.0 62-63 32.57843966296222 35.5 31.0 39.0 21.0 40.0 64-65 32.12759555466288 35.0 31.0 38.5 19.5 40.0 66-67 31.194555992936515 34.5 29.5 38.0 13.5 40.0 68-69 29.26895304473013 34.0 26.0 37.0 7.5 39.0 70-71 28.599456337027547 33.0 25.0 36.0 7.5 39.0 72-73 29.163721701188777 33.0 26.5 36.0 7.5 39.0 74-75 29.280033765184847 33.0 27.5 36.0 8.0 38.5 76-77 28.844618529743254 32.5 27.0 35.0 7.5 37.0 78-79 29.38358793681416 33.5 29.0 35.0 7.0 37.0 80-81 29.203446539239128 34.0 29.0 35.0 6.5 37.0 82-83 28.760695755556778 33.0 28.0 35.0 2.0 36.0 84-85 28.398286111967536 33.0 27.0 35.0 2.0 36.0 86-87 28.009731904091183 33.0 27.0 35.0 2.0 35.5 88-89 27.54690729584707 33.0 26.0 35.0 2.0 35.0 90-91 27.052225163207634 32.0 25.5 34.5 2.0 35.0 92-93 26.54808319250292 32.0 25.0 34.0 2.0 35.0 94-95 25.7220674971077 32.0 23.0 34.0 2.0 35.0 96-97 25.187072684698933 31.0 20.0 34.0 2.0 35.0 98-99 24.502507015934626 31.0 17.0 34.0 2.0 35.0 100-101 23.01351183082099 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17988493843832742 1101 2 0.2855062471614538 1101 3 0.2674831426330009 1101 4 0.2868155627105793 1101 5 0.2742078221368587 1101 6 0.17010020056256536 1101 7 0.1743368317415488 1101 8 0.19528035597855364 1101 9 0.40018432628531286 1101 10-11 0.2943299427983561 1101 12-13 0.3469400758460779 1101 14-15 0.3806860596351953 1101 16-17 0.3760080473403207 1101 18-19 0.2898711534817835 1101 20-21 0.37055206079502767 1101 22-23 0.2971975321374103 1101 24-25 0.26838903798350344 1101 26-27 0.2150971218871618 1101 28-29 0.20410795440687934 1101 30-31 0.1034024878553268 1101 32-33 0.0507036078768337 1101 34-35 0.18874963449750481 1101 36-37 0.4300087323138513 1101 38-39 0.49357503108973333 1101 40-41 0.3736855882006367 1101 42-43 0.2642211337712723 1101 44-45 0.14518132215555823 1101 46-47 0.20969021676545196 1101 48-49 0.2980616946352441 1101 50-51 0.37727929180618247 1101 52-53 0.16414569780015142 1101 54-55 0.09321729646163135 1101 56-57 0.13733997696855482 1101 58-59 0.07080403298442661 1101 60-61 0.17183732921368033 1101 62-63 0.1755407415169543 1101 64-65 0.037958490030320036 1101 66-67 0.01358076047332446 1101 68-69 -8.910587268274838 1101 70-71 -6.450645457586273 1101 72-73 -1.6090913627416654 1101 74-75 -0.48718807277465004 1101 76-77 -0.37618732883230876 1101 78-79 -0.4025690746739201 1101 80-81 -0.28701898551000227 1101 82-83 -0.3133595002462677 1101 84-85 -0.24360759989228242 1101 86-87 -0.09053015708545686 1101 88-89 0.016217480421513386 1101 90-91 -0.28742328936050043 1101 92-93 -0.4766675154702753 1101 94-95 -0.16973532725429052 1101 96-97 -0.5200689166646129 1101 98-99 -0.43008205717268666 1101 100-101 -0.5107524272535464 1102 1 0.19104154445479438 1102 2 0.11872147101439623 1102 3 0.1868801296428657 1102 4 0.14048936376222798 1102 5 0.34507243719236413 1102 6 0.17725294885271836 1102 7 0.31727804040755103 1102 8 0.20343402398442834 1102 9 0.2995539758095944 1102 10-11 0.2677602284539802 1102 12-13 0.2316371116141127 1102 14-15 0.3895659131388953 1102 16-17 0.35605601135586085 1102 18-19 0.30936735603243903 1102 20-21 0.28589618150065377 1102 22-23 0.33047918586569835 1102 24-25 0.21887964246675295 1102 26-27 0.24845899586070885 1102 28-29 0.2388111251903311 1102 30-31 0.038015117974005364 1102 32-33 0.10417142981042815 1102 34-35 -0.06356244354132201 1102 36-37 -0.07035033600804041 1102 38-39 0.15620284242029925 1102 40-41 0.23459119517545446 1102 42-43 0.17225689500863695 1102 44-45 0.22613946847227595 1102 46-47 0.29636957796546426 1102 48-49 0.23879377904876264 1102 50-51 -0.052219185887231845 1102 52-53 0.059237917992469136 1102 54-55 -0.20906770165493782 1102 56-57 -0.09711849076573742 1102 58-59 -0.19878249559491223 1102 60-61 -0.21748922853469566 1102 62-63 -0.06441893909021701 1102 64-65 -0.047414005568747086 1102 66-67 0.23858906867932816 1102 68-69 -4.690863837631454 1102 70-71 0.10769066899751678 1102 72-73 0.5706645888493078 1102 74-75 0.3167379972489499 1102 76-77 0.07037319908666007 1102 78-79 -0.16311530925902318 1102 80-81 -0.08699485683422381 1102 82-83 -0.041103882235582034 1102 84-85 -0.16900491815720287 1102 86-87 -0.2525279220081238 1102 88-89 -0.2655781759875744 1102 90-91 -0.27360698424239516 1102 92-93 -0.21387556117631945 1102 94-95 0.016784970703554336 1102 96-97 -0.4769676610076061 1102 98-99 -0.4118057925993419 1102 100-101 -0.4676661066923735 1103 1 0.24440273790884248 1103 2 0.25748975268978924 1103 3 0.21517822198522296 1103 4 0.1920606976339414 1103 5 0.2949158332150503 1103 6 0.31144104904711867 1103 7 0.21404172128418963 1103 8 0.29770951349635055 1103 9 0.3072920958000722 1103 10-11 0.4391720439733362 1103 12-13 0.3963156375274153 1103 14-15 0.4737059121674392 1103 16-17 0.38419807630588565 1103 18-19 0.4374760664145043 1103 20-21 0.4304851759958339 1103 22-23 0.3424898006206121 1103 24-25 0.27007060496880086 1103 26-27 0.3670627900694896 1103 28-29 0.3209649636563725 1103 30-31 0.23085667556418343 1103 32-33 0.27321601450674393 1103 34-35 0.08776560640981046 1103 36-37 0.16825110293842016 1103 38-39 0.38223900932523236 1103 40-41 0.30313981194806416 1103 42-43 0.20043530228520723 1103 44-45 0.01015728390008519 1103 46-47 0.20096328298528476 1103 48-49 0.11296292881653613 1103 50-51 -0.054418420811366275 1103 52-53 -0.031852813071864716 1103 54-55 -0.13305431859318873 1103 56-57 0.10959998235334467 1103 58-59 0.07381675302748647 1103 60-61 -0.2964293919423753 1103 62-63 -0.1398372165547883 1103 64-65 -0.31349723229210014 1103 66-67 0.005202753592058684 1103 68-69 -4.175645051583974 1103 70-71 0.3375319473807856 1103 72-73 0.6882690564394665 1103 74-75 0.21950733494848862 1103 76-77 0.08378011048861111 1103 78-79 -0.18537655677228315 1103 80-81 -0.11864404365719494 1103 82-83 -0.19102515217624827 1103 84-85 -0.25193653482812906 1103 86-87 -0.3480822944966988 1103 88-89 -0.38447113630420304 1103 90-91 -0.5224921659033548 1103 92-93 -0.5638408961879229 1103 94-95 -0.5687027520548149 1103 96-97 -0.7783376269401714 1103 98-99 -0.27787977649060736 1103 100-101 -0.38780008757141715 1104 1 0.23535816371727591 1104 2 0.27790055261132807 1104 3 0.19918555951726447 1104 4 0.2216332928018332 1104 5 0.24803888040925415 1104 6 0.18210338511262592 1104 7 0.14924546412716921 1104 8 0.22066576440607832 1104 9 0.27791531882586185 1104 10-11 0.20524697200887942 1104 12-13 0.22977707070407405 1104 14-15 0.32477099616433236 1104 16-17 0.4010082502163925 1104 18-19 0.1681202138202309 1104 20-21 0.33008812551370426 1104 22-23 0.21015912053103847 1104 24-25 0.21055519849594617 1104 26-27 0.3624803744758154 1104 28-29 0.29304822130200847 1104 30-31 0.25365768869142613 1104 32-33 0.27448006226951094 1104 34-35 0.22799985286985702 1104 36-37 0.37713767326793146 1104 38-39 0.44773134028530137 1104 40-41 0.38000064559385294 1104 42-43 0.40726611890779196 1104 44-45 0.4203253080540321 1104 46-47 0.3798706107179868 1104 48-49 0.34225397419053394 1104 50-51 0.3709527524904388 1104 52-53 0.3326294434553674 1104 54-55 0.2380350731712113 1104 56-57 0.31405306686095713 1104 58-59 0.19883722454623154 1104 60-61 0.19272622685070218 1104 62-63 0.33594803379818217 1104 64-65 0.41253532075380406 1104 66-67 0.5753963235027797 1104 68-69 -3.9408910833982773 1104 70-71 0.7133757913948138 1104 72-73 1.1107001738900735 1104 74-75 0.8554393157026468 1104 76-77 0.551117770712505 1104 78-79 0.2552834466785683 1104 80-81 0.22054578850418238 1104 82-83 0.11140559084163115 1104 84-85 0.27398728093140434 1104 86-87 0.34041902841458693 1104 88-89 0.20483300746851896 1104 90-91 0.3552620439769818 1104 92-93 0.17577192688468912 1104 94-95 0.6166128567803817 1104 96-97 0.16747715131216978 1104 98-99 0.2802082317280217 1104 100-101 0.284149875398537 1105 1 0.17557432877424972 1105 2 0.18456314596637213 1105 3 0.28489765359212527 1105 4 0.17242010086300041 1105 5 0.24909165175870385 1105 6 0.3026699148707692 1105 7 0.16097387274687947 1105 8 0.19364230359539647 1105 9 0.2995677655084137 1105 10-11 0.2237221357899557 1105 12-13 0.11022088196597934 1105 14-15 0.3348549383312971 1105 16-17 0.3054697078363162 1105 18-19 0.18341293650291846 1105 20-21 0.3936859478893595 1105 22-23 0.2440777594592518 1105 24-25 0.18337116800734066 1105 26-27 0.4248773353327522 1105 28-29 0.44324409966973377 1105 30-31 0.3048869868505122 1105 32-33 0.27941936859519245 1105 34-35 0.20915970505923553 1105 36-37 -6.337927046473624E-5 1105 38-39 0.2644680086758058 1105 40-41 0.2510424782379843 1105 42-43 0.1900942151737226 1105 44-45 0.15043971020569558 1105 46-47 0.24509258290631664 1105 48-49 0.13380366626281415 1105 50-51 0.20868053680192844 1105 52-53 0.20841556930339067 1105 54-55 0.1215124033949806 1105 56-57 0.04063560167213609 1105 58-59 0.14871136454169687 1105 60-61 0.11908439673575089 1105 62-63 0.17591189646900318 1105 64-65 0.1082782442068293 1105 66-67 0.6111744924160902 1105 68-69 1.8917566257300038 1105 70-71 2.22405159505853 1105 72-73 1.24924601495238 1105 74-75 0.720795650600742 1105 76-77 0.656987848711097 1105 78-79 0.5362206477943374 1105 80-81 0.5959854831921696 1105 82-83 0.6742634615512628 1105 84-85 0.3491581836424906 1105 86-87 0.30662626613025523 1105 88-89 0.40986368043683896 1105 90-91 0.5559500975263809 1105 92-93 0.5090792196433149 1105 94-95 0.8593328106161842 1105 96-97 0.28947483112192174 1105 98-99 0.5429340026787877 1105 100-101 0.697153889474091 1106 1 0.36742285545149045 1106 2 0.2812752633758784 1106 3 0.2384919793316378 1106 4 0.15228526017350674 1106 5 0.3945566068688464 1106 6 0.3361469394774801 1106 7 0.24034535770564247 1106 8 0.3214650314385139 1106 9 0.2962880110051671 1106 10-11 0.35862129046888214 1106 12-13 0.36733794923802776 1106 14-15 0.5121356510932458 1106 16-17 0.522058278319669 1106 18-19 0.5118115464574018 1106 20-21 0.3759695696509624 1106 22-23 0.3867474368454893 1106 24-25 0.4200414614170356 1106 26-27 0.45314133275457635 1106 28-29 0.5726863845463512 1106 30-31 0.5774684531004155 1106 32-33 0.4531853966128594 1106 34-35 0.5549032254051127 1106 36-37 0.6501743316320088 1106 38-39 0.6977118142177403 1106 40-41 0.6103260372564492 1106 42-43 0.5250099112231865 1106 44-45 0.6112327405847822 1106 46-47 0.6746883745534973 1106 48-49 0.7581566277385363 1106 50-51 0.6768138003573014 1106 52-53 0.6489775053282116 1106 54-55 0.5928491315118904 1106 56-57 0.6453025497322287 1106 58-59 0.920691780129367 1106 60-61 0.8240077360391922 1106 62-63 0.8421120299407576 1106 64-65 0.7780297814135011 1106 66-67 1.2064385269134021 1106 68-69 2.737078412426179 1106 70-71 2.904432388816158 1106 72-73 1.8117720390913732 1106 74-75 1.3531847235995222 1106 76-77 1.0033776945911583 1106 78-79 0.7990523307932307 1106 80-81 0.9444376529636713 1106 82-83 0.8853206547658772 1106 84-85 0.8760389802520798 1106 86-87 0.8920606624843117 1106 88-89 1.0687581607526049 1106 90-91 0.7140039625031669 1106 92-93 0.6576663584425226 1106 94-95 1.033640711116984 1106 96-97 0.6949925902795684 1106 98-99 0.8190258757323257 1106 100-101 1.0879993816654228 1107 1 0.18045331414138133 1107 2 0.15498012695970687 1107 3 0.11649231789124315 1107 4 -0.01881284039036757 1107 5 0.03853272674228947 1107 6 0.06958159747390624 1107 7 -0.06640277729792388 1107 8 0.07892282823270591 1107 9 0.029981696284828274 1107 10-11 0.0766741057671112 1107 12-13 -0.06285748137477043 1107 14-15 0.18770069539785794 1107 16-17 0.1021050053822492 1107 18-19 0.053188418750380606 1107 20-21 0.014853432032317926 1107 22-23 -0.0034333860878419387 1107 24-25 0.13127237366004607 1107 26-27 0.012791667811846708 1107 28-29 0.09233196225746099 1107 30-31 0.24310545591395538 1107 32-33 0.3213853831872129 1107 34-35 0.14443655309578673 1107 36-37 0.058620955959270304 1107 38-39 0.2901323840485759 1107 40-41 0.059590983495517946 1107 42-43 0.08915382240382286 1107 44-45 0.184837714492339 1107 46-47 0.2478442462277286 1107 48-49 0.3180922576345182 1107 50-51 0.27121611131845924 1107 52-53 0.3852100228107318 1107 54-55 0.1377626382889261 1107 56-57 0.2312397761118561 1107 58-59 0.10366755852459164 1107 60-61 0.30221588014884304 1107 62-63 0.36243946961293716 1107 64-65 0.25544406162411093 1107 66-67 0.7704948795757645 1107 68-69 2.188962817670351 1107 70-71 2.3785515501222996 1107 72-73 1.0465131431459156 1107 74-75 0.49256146747334384 1107 76-77 0.2993904255997144 1107 78-79 0.5936845558303006 1107 80-81 0.5046454439206265 1107 82-83 0.4813920499816824 1107 84-85 0.4006367218260287 1107 86-87 0.28482300239815217 1107 88-89 0.2943836934002597 1107 90-91 0.4085630032120413 1107 92-93 0.22446912996148782 1107 94-95 0.5365990945532495 1107 96-97 0.4001119179468908 1107 98-99 0.5236566998331007 1107 100-101 0.47729622839131736 1108 1 -0.21848596059799874 1108 2 -0.19650866659567967 1108 3 -0.08986940896975781 1108 4 -0.16735910043072266 1108 5 -0.08368120310769456 1108 6 -0.06919593341297059 1108 7 -0.17172018329038963 1108 8 -0.20594082191375662 1108 9 -0.26495649541931243 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-1.0859252233267043 2316 50-51 -0.8649981140009899 2316 52-53 -0.7999711678708934 2316 54-55 -0.9058790869909146 2316 56-57 -0.7969003078071211 2316 58-59 -0.9681839310878004 2316 60-61 -0.6030981606525572 2316 62-63 -0.7504001278755403 2316 64-65 -0.6003633957285963 2316 66-67 -0.025912275864492074 2316 68-69 1.2913175835225559 2316 70-71 1.2709468062365303 2316 72-73 0.3686745035380561 2316 74-75 -0.0925962803404552 2316 76-77 -0.3200756531827267 2316 78-79 -0.47902038380881606 2316 80-81 -0.5781563136822818 2316 82-83 -0.5529418484772144 2316 84-85 -0.472748745180386 2316 86-87 -0.32918387705725394 2316 88-89 -0.4503796524695467 2316 90-91 -0.7127212482222554 2316 92-93 -0.7667824671063883 2316 94-95 -0.4381155578513187 2316 96-97 -0.41271928908351896 2316 98-99 -0.5545965652434397 2316 100-101 -0.8857021756211232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 20.0 4 58.0 5 112.0 6 253.0 7 596.0 8 1435.0 9 2645.0 10 3996.0 11 6013.0 12 8333.0 13 10782.0 14 13143.0 15 15897.0 16 19442.0 17 23002.0 18 21417.0 19 19192.0 20 19190.0 21 19722.0 22 21660.0 23 24767.0 24 29567.0 25 35924.0 26 44112.0 27 54307.0 28 66620.0 29 80487.0 30 95484.0 31 113647.0 32 135449.0 33 163836.0 34 202011.0 35 253075.0 36 314175.0 37 331164.0 38 179321.0 39 14157.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.382736275139976 19.282898909500997 14.446776593789012 23.887588221570017 2 16.078086298770348 20.63167018058992 38.64643079092491 24.643812729714824 3 19.55468164650265 24.028504673096464 29.981206975295294 26.435606705105585 4 13.41264185919176 16.798072851435318 36.21825665378404 33.57102863558888 5 13.65123267657959 37.46118901382036 34.35272805891978 14.534850250680273 6 32.87279671200818 36.29920964359336 16.663326251653505 14.16466739274496 7 28.722027412144634 32.46997145865343 20.362777444447143 18.445223684754794 8 26.690700587074133 35.307560407826124 19.250888798768795 18.750850206330952 9 26.302985178371692 16.166436178129047 19.52837055904451 38.00220808445475 10-11 25.186491026298718 26.349829148505833 27.249096915632663 21.21458290956278 12-13 26.018680445659502 23.42537523150132 27.64116196926336 22.914782353575813 14-15 22.597172986658105 25.01250522916872 25.633566295198463 26.756755488974715 16-17 22.438258153710482 28.2298211603723 26.44345046451907 22.888470221398155 18-19 22.467067431010154 26.73438893245641 28.582177798985853 22.216365837547585 20-21 23.025122610946863 25.864332016073217 28.80147239745179 22.309072975528128 22-23 22.71083944309193 25.887103686579938 28.68823237679524 22.71382449353289 24-25 22.63794990059778 26.190182761923364 28.6548973184854 22.516970018993458 26-27 22.300269678790556 26.490290881240693 28.49042989971906 22.719009540249687 28-29 22.36102013802891 26.81148017753397 28.365968505118943 22.46153117931818 30-31 22.51044453172208 26.397980901549552 28.368614677909278 22.722959888819094 32-33 22.317281765213355 26.387068793835976 28.27545679825225 23.020192642698415 34-35 22.78695697415174 26.501162514989872 28.21941169747658 22.4924688133818 36-37 22.906813684999136 26.0498001122701 28.3214126395639 22.72197356316687 38-39 22.435418872940403 26.42273976192946 28.274409017432923 22.867432347697218 40-41 22.781405274523276 26.25816720380086 28.321176576808483 22.63925094486739 42-43 22.535362485082644 26.30639326238175 28.148066405420387 23.010177847115216 44-45 22.492797200453925 26.425518282486337 28.3644421279948 22.717242389064936 46-47 22.82474288409456 26.61639950230812 27.8003384816983 22.758519131899018 48-49 22.55781880857592 26.731426976262945 27.698008317422833 23.0127458977383 50-51 22.60164208336419 26.91809196324168 27.71646861800743 22.763797335386702 52-53 22.90817780979384 26.578073869484843 27.617690336743383 22.896057983977933 54-55 22.45935726900988 26.73834909800639 27.776291416669974 23.02600221631376 56-57 22.480600644693563 26.81486698422862 27.641817760277483 23.062714610800334 58-59 22.42357566900678 26.687574916668034 27.554598404009745 23.334251010315445 60-61 22.209600884000707 26.7370978565499 27.850756217655746 23.20254504179364 62-63 22.22687283845849 26.670533098504457 27.901017923470235 23.201576139566818 64-65 22.037759923851553 26.786141886614466 27.733114531640773 23.442983657893205 66-67 21.894035752512046 26.833070091290462 27.774761907609303 23.49813224858819 68-69 21.969556659296106 26.456428287114047 28.21645600219768 23.35755905139217 70-71 21.908619357214267 26.530977922303116 27.856217741365764 23.70418497911685 72-73 21.963919781957582 26.64794818352459 27.63216386249007 23.755968172027757 74-75 22.17236164935173 26.928974988429456 27.108174456077787 23.79048890614103 76-77 22.23261875257277 26.671600693607587 27.214926640119614 23.88085391370003 78-79 22.275368304697064 26.733938917211592 27.188691882104116 23.802000895987227 80-81 22.47066241106292 26.73053768113377 26.96531515213414 23.83348475566917 82-83 22.33327796774311 26.74548196528087 26.98066134659492 23.940578720381097 84-85 22.286026926191564 26.740648092987513 26.915061515912775 24.058263464908148 86-87 22.419032462069023 26.75776327589582 26.758999958635094 24.064204303400068 88-89 22.35577522754153 26.708376483441555 26.633195615458604 24.302652673558317 90-91 22.20473589305616 26.713614653093714 26.711375853329187 24.370273600520935 92-93 22.28788550764192 26.491603268286024 26.82742989219432 24.39308133187773 94-95 21.952772546263162 26.265219226424364 27.116202786736437 24.665805440576037 96-97 22.21313046851392 25.91109288495278 27.139823139444395 24.7359535070889 98-99 22.133511390034382 26.161304954871284 26.777963478614325 24.92722017648001 100-101 21.958537776384617 25.980079197026594 27.064379036883977 24.997003989704815 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 493.0 1 340.5 2 220.5 3 283.5 4 291.5 5 294.5 6 478.0 7 972.0 8 1515.0 9 1774.0 10 1691.5 11 1530.0 12 1613.0 13 1766.5 14 1924.5 15 2135.5 16 2325.5 17 2542.0 18 2813.0 19 3188.0 20 3669.5 21 4295.0 22 5216.0 23 6356.0 24 7788.0 25 9697.0 26 12348.0 27 15719.0 28 18850.0 29 21984.0 30 25285.5 31 29801.0 32 36297.5 33 39537.0 34 40313.0 35 43204.0 36 47678.5 37 52065.0 38 55630.5 39 58805.0 40 62478.5 41 66720.0 42 69642.0 43 72760.0 44 76792.5 45 80889.0 46 83870.0 47 87322.5 48 90471.5 49 89991.0 50 88752.5 51 88500.5 52 88523.0 53 89336.0 54 89505.5 55 85152.0 56 78606.5 57 71675.0 58 65037.5 59 57264.0 60 46842.0 61 38162.0 62 30708.0 63 22566.0 64 16058.5 65 11897.5 66 8847.0 67 6841.5 68 5197.0 69 3681.0 70 2540.5 71 1727.0 72 1232.0 73 949.5 74 702.5 75 479.5 76 335.0 77 210.5 78 122.5 79 65.5 80 27.5 81 15.5 82 13.5 83 9.5 84 5.5 85 3.5 86 2.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02115121455305906 2 0.022856957662176724 3 0.0 4 0.0 5 0.0 6 4.264357772794165E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.543665104632415E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.264357772794165E-5 26-27 2.9850504409559156E-4 28-29 1.2793073318382494E-4 30-31 5.117229327352997E-4 32-33 0.0023880403527647325 34-35 0.00439228850597799 36-37 0.1046473397443688 38-39 0.09671563428697166 40-41 0.10637440464235046 42-43 0.10786692986282839 44-45 0.10036166018271067 46-47 0.0939864453123834 48-49 0.0949246040223981 50-51 0.09392247994579148 52-53 0.07466890460162583 54-55 0.06345364365917718 56-57 0.034306758282129056 58-59 0.014093702439084714 60-61 0.0095734831999229 62-63 0.0020895353086691406 64-65 0.005351769004856677 66-67 0.15782388117111204 68-69 0.4191224036990745 70-71 0.4010202049535633 72-73 0.1865016871931528 74-75 0.030021078720470922 76-77 0.032153257606868005 78-79 0.005095907538489027 80-81 0.0013859162761581036 82-83 2.1321788863970825E-5 84-85 6.822972436470664E-4 86-87 0.0015351687982058993 88-89 3.198268329595624E-4 90-91 5.330447215992707E-4 92-93 0.0025159710859485574 94-95 0.07880533164123617 96-97 0.017206683613224458 98-99 0.019083001033253888 100-101 0.009978597188338345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2345019.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.53516800163682 #Duplication Level Percentage of deduplicated Percentage of total 1 72.2370751980587 37.22749806278828 2 14.492660285280575 14.937633651851687 3 5.395194237188032 8.341267242448442 4 2.606008176665558 5.3720427679239515 5 1.488509799869043 3.835530130416697 6 0.9310727865848883 2.878979548704262 7 0.5893960207993522 2.126223606597359 8 0.4065822120532663 1.6762626083713765 9 0.30263407272553106 1.4036668002836836 >10 1.427050009729785 12.909281102204226 >50 0.08123543676330242 2.8752436214568635 >100 0.038030086659964615 3.5935804250657455 >500 0.0026482487982225477 0.9554735751607004 >1k 0.0017379132738329834 1.3393604496870672 >5k 1.6551554988885556E-4 0.5279564070397467 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6721 0.28660748590949586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5658 0.24127736278469386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3857 0.16447627929667094 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.264357772794165E-5 2 0.0 0.0 0.0 4.264357772794165E-5 4.264357772794165E-5 3 0.0 0.0 0.0 4.264357772794165E-5 4.264357772794165E-5 4 0.0 0.0 0.0 8.52871554558833E-5 4.264357772794165E-5 5 0.0 0.0 0.0 8.52871554558833E-5 4.264357772794165E-5 6 1.2793073318382496E-4 0.0 0.0 8.52871554558833E-5 4.264357772794165E-5 7 2.1321788863970826E-4 0.0 0.0 1.2793073318382496E-4 4.264357772794165E-5 8 2.1321788863970826E-4 0.0 0.0 1.2793073318382496E-4 4.264357772794165E-5 9 2.1321788863970826E-4 0.0 0.0 1.705743109117666E-4 4.264357772794165E-5 10-11 2.1321788863970826E-4 0.0 0.0 1.705743109117666E-4 4.264357772794165E-5 12-13 2.1321788863970826E-4 0.0 0.0 1.705743109117666E-4 3.62470410687504E-4 14-15 2.558614663676499E-4 0.0 0.0 1.705743109117666E-4 4.2643577727941647E-4 16-17 4.264357772794165E-4 0.0 0.0 1.705743109117666E-4 4.6907935500735816E-4 18-19 4.90401143871329E-4 0.0 0.0 1.9189609977573742E-4 5.117229327352998E-4 20-21 5.970100881911831E-4 0.0 0.0 3.62470410687504E-4 6.609754547830956E-4 22-23 5.970100881911831E-4 0.0 0.0 5.543665104632415E-4 7.675843991029497E-4 24-25 7.24940821375008E-4 0.0 0.0 8.102279768308913E-4 7.675843991029497E-4 26-27 7.24940821375008E-4 0.0 0.0 0.0018976392088934035 7.889061879669205E-4 28-29 7.24940821375008E-4 0.0 0.0 0.006929581380790518 8.102279768308913E-4 30-31 7.24940821375008E-4 0.0 0.0 0.024178908571742916 8.528715545588329E-4 32-33 7.462626102389788E-4 0.0 0.0 0.061193534039596265 8.955151322867747E-4 34-35 7.889061879669205E-4 0.0 0.0 0.11899690364982117 8.955151322867747E-4 36-37 8.52871554558833E-4 0.0 0.0 0.2145611613381384 8.955151322867747E-4 38-39 8.955151322867747E-4 0.0 0.0 0.3659885058500592 9.381587100147163E-4 40-41 8.955151322867747E-4 0.0 0.0 0.5321065628892558 9.381587100147163E-4 42-43 8.955151322867747E-4 0.0 0.0 0.7013376011025922 9.381587100147163E-4 44-45 9.381587100147163E-4 0.0 0.0 0.9067730368069513 9.80802287742658E-4 46-47 0.0010021240766066287 0.0 0.0 1.1448947748397775 0.0010447676543345705 48-49 0.0010234458654705997 0.0 0.0 1.3970036063673685 0.0011726983875183954 50-51 0.0010234458654705997 0.0 0.0 1.6704342267589303 0.0012366637541103079 52-53 0.0010234458654705997 0.0 0.0 1.927894827291378 0.0013219509095661911 54-55 0.0010447676543345705 0.0 0.0 2.204864864634359 0.0013219509095661911 56-57 0.0010660894431985413 0.0 0.0 2.519275963222473 0.0013645944872941328 58-59 0.0010660894431985413 0.0 0.0 2.8401902074140977 0.0014072380650220744 60-61 0.001108733020926483 0.0 0.0 3.1749636143673037 0.0014072380650220744 62-63 0.0011940201763823662 0.0 0.0 3.4944706204939067 0.001449881642750016 64-65 0.001215341965246337 0.0 0.0 3.8320158599994283 0.001449881642750016 66-67 0.0013219509095661911 0.0 0.0 4.187492723939551 0.0014712034316139869 68-69 0.0013219509095661911 0.0 0.0 4.58318248167712 0.0014925252204779577 70-71 0.0013859162761581036 0.0 0.0 5.032006137263707 0.0015351687982058993 72-73 0.0014925252204779577 0.0 0.0 5.489315864818153 0.0016417777425257536 74-75 0.0014925252204779577 0.0 0.0 5.942638417855036 0.001727064897981637 76-77 0.0014925252204779577 0.0 0.0 6.423828548937131 0.0017697084757095785 78-79 0.0014925252204779577 0.0 0.0 6.963440381506504 0.001833673842301491 80-81 0.0016204559536617826 0.0 0.0 7.536335526492536 0.0018763174200294326 82-83 0.0016204559536617826 0.0 0.0 8.12658660761384 0.0021321788863970827 84-85 0.0016204559536617826 0.0 0.0 8.685899773093523 0.0021748224641250243 86-87 0.0016630995313897242 0.0 0.0 9.256684060982021 0.002238787830716937 88-89 0.001705743109117666 0.0 0.0 9.86499469727111 0.002345396775036791 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4235 0.0 48.522816 1 GTATCAA 7530 0.0 39.453983 1 TATCAAC 9320 0.0 31.460356 2 ATCAACG 9435 0.0 30.765337 3 TCAACGC 9540 0.0 30.377007 4 CAACGCA 9830 0.0 29.480839 5 AACGCAG 10225 0.0 28.34197 6 ACGCAGA 11685 0.0 24.638378 7 CGCAGAG 11745 0.0 24.310596 8 GTACATG 8305 0.0 23.714827 1 TACATGG 8205 0.0 23.072006 2 GCAGAGT 12840 0.0 21.609413 9 ACATGGG 8495 0.0 21.272264 3 GAGTACT 7250 0.0 20.640621 12-13 TAAGGTG 1145 0.0 19.883274 5 AGAGTAC 11005 0.0 19.588474 10-11 GTACTTT 7700 0.0 19.249554 14-15 CAGAGTA 12130 0.0 19.15985 10-11 GTATAGG 540 0.0 18.455976 1 TGGTATC 2260 0.0 18.264252 2 >>END_MODULE