##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939824_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 911479 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3369534569639 31.0 31.0 34.0 30.0 34.0 2 31.489057893818728 31.0 31.0 34.0 30.0 34.0 3 31.505102147169602 31.0 31.0 34.0 30.0 34.0 4 35.28557432480617 37.0 35.0 37.0 33.0 37.0 5 35.07145529408796 35.0 35.0 37.0 32.0 37.0 6 35.16347057913567 37.0 35.0 37.0 32.0 37.0 7 35.07122819066594 37.0 35.0 37.0 32.0 37.0 8 35.09009971705327 37.0 35.0 37.0 32.0 37.0 9 36.556113744803774 39.0 35.0 39.0 32.0 39.0 10-11 36.435224508737996 38.0 35.0 39.0 32.0 39.0 12-13 36.32904488199948 38.0 35.0 39.0 32.0 39.0 14-15 37.01884300131983 39.0 36.0 40.0 31.0 41.0 16-17 36.99777614185297 38.5 36.0 40.0 31.5 41.0 18-19 37.23420726094622 39.0 36.0 40.0 32.0 41.0 20-21 37.251893899914315 39.0 36.0 40.0 31.5 41.0 22-23 37.11834008243744 39.0 36.0 40.0 31.0 41.0 24-25 36.90978837691269 39.0 35.0 40.0 31.0 41.0 26-27 36.39507931614442 38.0 35.0 40.0 30.0 41.0 28-29 36.06557035323908 38.0 34.5 40.0 29.5 41.0 30-31 35.72844848866512 38.0 34.0 40.0 29.5 41.0 32-33 35.52612896183017 38.0 34.0 40.0 29.0 41.0 34-35 35.2840142230375 38.0 34.0 40.0 28.5 41.0 36-37 35.04243048934754 38.0 34.0 40.0 27.0 41.0 38-39 34.72816817502104 37.0 33.0 40.0 25.5 41.0 40-41 34.45256500698316 37.0 33.0 40.0 25.0 41.0 42-43 34.16441739195308 37.0 33.0 40.0 24.0 41.0 44-45 33.76803305397053 37.0 33.0 40.0 23.0 41.0 46-47 33.196270018288956 37.0 33.0 40.0 19.5 41.0 48-49 32.71135429340665 37.0 32.0 40.0 14.0 41.0 50-51 32.43813406562302 36.5 32.0 40.0 10.5 41.0 52-53 31.94053181697 36.0 31.0 40.0 7.5 41.0 54-55 31.687543541869864 35.0 31.0 39.0 4.0 41.0 56-57 31.44124823501145 35.0 31.0 39.0 2.0 41.0 58-59 31.131576810875508 35.0 30.0 39.0 2.0 40.0 60-61 30.783891345823655 35.0 29.5 39.0 2.0 40.0 62-63 30.3684796907005 35.0 29.0 38.5 2.0 40.0 64-65 29.75007542686118 35.0 28.0 38.0 2.0 40.0 66-67 28.930940263023064 34.0 26.5 37.5 2.0 39.5 68-69 27.3585151166401 33.0 22.5 36.0 2.0 39.0 70-71 26.70195418654736 33.0 20.5 36.0 2.0 39.0 72-73 27.04626711092631 33.0 23.5 35.0 2.0 38.0 74-75 27.03736509563029 33.0 24.5 35.0 2.0 37.0 76-77 26.37153187292302 31.5 24.0 34.5 2.0 36.0 78-79 26.757552285900168 33.0 25.0 35.0 2.0 36.0 80-81 26.632750178555952 33.0 24.5 35.0 2.0 36.0 82-83 26.234085480850354 33.0 23.5 35.0 2.0 36.0 84-85 25.925148028643555 33.0 23.0 35.0 2.0 35.0 86-87 25.477253452904563 32.0 20.0 35.0 2.0 35.0 88-89 24.911368775363997 32.0 19.0 34.5 2.0 35.0 90-91 24.348794651330422 31.0 13.0 34.0 2.0 35.0 92-93 23.966226320079784 31.0 6.0 34.0 2.0 35.0 94-95 23.309908401619786 31.0 2.0 34.0 2.0 35.0 96-97 22.739115218233223 31.0 2.0 34.0 2.0 35.0 98-99 22.09983773625064 30.5 2.0 34.0 2.0 35.0 100-101 20.80006451053727 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.18719379560117488 1101 2 0.20212729158479092 1101 3 0.3130143685572371 1101 4 0.3128311516644544 1101 5 0.2652369553375422 1101 6 0.12769742474216628 1101 7 0.05650166771644649 1101 8 0.31517627992995756 1101 9 0.39730906458893145 1101 10-11 0.3697198831900721 1101 12-13 0.4165606409946818 1101 14-15 0.29303368102002736 1101 16-17 0.45457175475964817 1101 18-19 0.25849162900725986 1101 20-21 0.12561683981081728 1101 22-23 0.28672823497827693 1101 24-25 0.31958259376202847 1101 26-27 0.3983621746050119 1101 28-29 0.3345571214009766 1101 30-31 0.28080046915612655 1101 32-33 0.3372627303532383 1101 34-35 0.3446611962658821 1101 36-37 0.5234217827716989 1101 38-39 0.6306881162344098 1101 40-41 0.6381384942482455 1101 42-43 0.4989645754098859 1101 44-45 0.6812571786398536 1101 46-47 0.6021256873252341 1101 48-49 0.4932727139098745 1101 50-51 0.6002856831334356 1101 52-53 0.3254123304303711 1101 54-55 0.6551856297004051 1101 56-57 0.8327283920281587 1101 58-59 0.5537474771162074 1101 60-61 0.452922466668074 1101 62-63 0.18696550565356418 1101 64-65 0.05127456939529651 1101 66-67 0.09910440519129438 1101 68-69 -8.093812644454307 1101 70-71 -5.102331763393767 1101 72-73 -1.110304478547647 1101 74-75 -0.21525181397393567 1101 76-77 -0.17610052066743265 1101 78-79 -0.5140106447251327 1101 80-81 -0.39916737846659345 1101 82-83 -0.055811961406032395 1101 84-85 -0.3072296966693031 1101 86-87 -0.1360336852316273 1101 88-89 -0.0139509544130334 1101 90-91 -0.43226593454112106 1101 92-93 -0.8556299131702012 1101 94-95 -0.25334622211105184 1101 96-97 -0.8362077896349476 1101 98-99 -0.8291800728567935 1101 100-101 -0.5764462867845452 1102 1 -0.025265654116473968 1102 2 0.025940820360471406 1102 3 0.20777587282252696 1102 4 0.23163046281027277 1102 5 0.22940051815543683 1102 6 0.22899102881121536 1102 7 0.08601025836885867 1102 8 0.15795332346393565 1102 9 0.33208018077299783 1102 10-11 0.30826167501193424 1102 12-13 0.2394129778924352 1102 14-15 0.3400760135123093 1102 16-17 0.38409836775976913 1102 18-19 0.26632734529545843 1102 20-21 0.15821125688798787 1102 22-23 0.28475578914544286 1102 24-25 0.11069557385734896 1102 26-27 0.22259622482729213 1102 28-29 0.09318582098845951 1102 30-31 0.10324830447192568 1102 32-33 0.007237499066441444 1102 34-35 0.1797066526317721 1102 36-37 -0.23059920325096783 1102 38-39 0.12654297626085054 1102 40-41 -0.022414591578183263 1102 42-43 -0.06697318741601066 1102 44-45 0.07831753347985426 1102 46-47 -0.18089125368161518 1102 48-49 -0.04162656119125785 1102 50-51 0.09045349121545598 1102 52-53 0.09079941040954864 1102 54-55 -0.25665104777966974 1102 56-57 -0.05699686729339959 1102 58-59 -0.11199804733411156 1102 60-61 0.05215952061496765 1102 62-63 -0.161318366347718 1102 64-65 -0.046905173485875196 1102 66-67 0.02248139265575233 1102 68-69 -4.078771994047802 1102 70-71 0.07071881932880686 1102 72-73 0.17926658957683017 1102 74-75 -0.17254685983080265 1102 76-77 -0.14643173136870047 1102 78-79 -0.251669341509146 1102 80-81 -0.1719436210073475 1102 82-83 -0.27559849670980796 1102 84-85 -0.6475597058807274 1102 86-87 -0.7038918293303063 1102 88-89 -0.7638688526067909 1102 90-91 -0.6657094044438026 1102 92-93 -1.060596071206163 1102 94-95 -0.9990729232204281 1102 96-97 -1.290048552180508 1102 98-99 -1.0683065897377269 1102 100-101 -1.0131397867244694 1103 1 0.13467426228895718 1103 2 0.13478355808251763 1103 3 0.1505658414745632 1103 4 0.1549716331342026 1103 5 0.25875004793599743 1103 6 0.16511955545698243 1103 7 0.05218058229780098 1103 8 0.13109856902297423 1103 9 0.23995635632158496 1103 10-11 0.336461779505143 1103 12-13 0.20686987444415905 1103 14-15 0.33111572093132935 1103 16-17 0.3203640417409588 1103 18-19 0.0768027893915999 1103 20-21 0.09528675323073088 1103 22-23 0.26133624891556195 1103 24-25 0.06479693226905425 1103 26-27 0.09363854457649978 1103 28-29 -0.08013575685897933 1103 30-31 -0.015214339206238492 1103 32-33 -0.07653993040064933 1103 34-35 -0.21066691058661746 1103 36-37 0.005686063206717051 1103 38-39 0.33510488848655484 1103 40-41 0.14041848052212913 1103 42-43 0.03767022951188892 1103 44-45 -0.030812560564029923 1103 46-47 -0.02436042818526829 1103 48-49 -0.2737582226332549 1103 50-51 -0.26979337398830694 1103 52-53 -0.2854022614817815 1103 54-55 -0.18056693074727548 1103 56-57 -0.39102899895483745 1103 58-59 -0.4389638258085107 1103 60-61 -0.5164729245260951 1103 62-63 -0.4693643433592136 1103 64-65 -0.5829945151786688 1103 66-67 -0.17117064496180134 1103 68-69 -3.605097751623557 1103 70-71 -0.08793452445384631 1103 72-73 0.19595937954548148 1103 74-75 0.06377759157985707 1103 76-77 -0.10060492886086436 1103 78-79 -0.33591699041196676 1103 80-81 -0.030858197809852328 1103 82-83 -0.2349245155185855 1103 84-85 -0.2689285669047621 1103 86-87 -0.4228638773971838 1103 88-89 -0.621039699001777 1103 90-91 -0.5443013584667895 1103 92-93 -0.7121438768487991 1103 94-95 -0.7699323798557458 1103 96-97 -1.3846279670185453 1103 98-99 -1.09663731074086 1103 100-101 -1.3276512809773457 1104 1 0.11618756605835756 1104 2 0.1271616068630088 1104 3 0.2471290343792205 1104 4 0.15017673291247036 1104 5 0.3090549259847819 1104 6 0.12537454437050854 1104 7 0.27928701245301113 1104 8 0.20959080849082312 1104 9 0.3486869549912086 1104 10-11 0.21004825400625293 1104 12-13 0.06741588331333759 1104 14-15 0.17496272758321396 1104 16-17 0.25762845415781044 1104 18-19 0.08739037254014903 1104 20-21 0.3278394139846128 1104 22-23 0.16541052829472136 1104 24-25 0.32549538016261437 1104 26-27 0.3952737995654161 1104 28-29 0.28390249147804525 1104 30-31 0.530087767223911 1104 32-33 0.3256219321270706 1104 34-35 -0.05376558020967792 1104 36-37 0.1613955975747885 1104 38-39 0.4356453541184777 1104 40-41 0.11997779316070734 1104 42-43 0.31770071731676097 1104 44-45 0.13274065451356876 1104 46-47 0.1185442281340201 1104 48-49 0.3194790279210693 1104 50-51 0.4101262490715669 1104 52-53 0.19802345913905484 1104 54-55 0.3081525814478425 1104 56-57 0.397399493284631 1104 58-59 0.577873757340047 1104 60-61 0.3483136608397537 1104 62-63 0.20325206462304735 1104 64-65 0.06758752473264096 1104 66-67 0.11325285836413812 1104 68-69 -2.9653943148164643 1104 70-71 0.7873842116880603 1104 72-73 0.9240635924057905 1104 74-75 1.0700137334867321 1104 76-77 0.8913158028464565 1104 78-79 0.6835397723374754 1104 80-81 0.6190725117245144 1104 82-83 0.4881907838162256 1104 84-85 0.3890536947360381 1104 86-87 0.5376544152857434 1104 88-89 0.5175135160758302 1104 90-91 0.47806560383919816 1104 92-93 0.4572990278075224 1104 94-95 0.7141862454214163 1104 96-97 -0.10838073863716602 1104 98-99 0.06431058282898405 1104 100-101 0.12337421791400871 1105 1 0.23278032289501738 1105 2 0.27682901262797444 1105 3 0.3033796942884166 1105 4 0.22894349460606378 1105 5 0.3472240739100272 1105 6 0.3256173909548181 1105 7 0.2888688939861126 1105 8 0.2886743265987306 1105 9 0.24985895372418554 1105 10-11 0.3813210868644461 1105 12-13 0.24250299132727804 1105 14-15 0.42938411919973163 1105 16-17 0.5983131759400919 1105 18-19 0.28362097120977836 1105 20-21 0.45191554110952126 1105 22-23 0.5101998852791993 1105 24-25 0.27994844742055847 1105 26-27 0.17064070907866835 1105 28-29 0.43580039032716655 1105 30-31 0.5905486478067488 1105 32-33 0.5089579050971906 1105 34-35 0.3343006218809208 1105 36-37 0.1289544614751108 1105 38-39 0.3712250183566823 1105 40-41 0.43763168398533736 1105 42-43 0.3906734762651354 1105 44-45 0.48980432255284967 1105 46-47 0.8022629484381056 1105 48-49 0.7324106085355666 1105 50-51 0.5998765394316123 1105 52-53 0.41005228397276383 1105 54-55 0.6302014675211183 1105 56-57 0.736487234811392 1105 58-59 0.6550567369620559 1105 60-61 0.7747337854306124 1105 62-63 1.0711118471169776 1105 64-65 1.026475181791028 1105 66-67 1.6714700476788913 1105 68-69 2.6822669669911576 1105 70-71 2.7222115794422557 1105 72-73 1.7159810245671245 1105 74-75 1.501277591579857 1105 76-77 0.9290758070698715 1105 78-79 0.9953600658650892 1105 80-81 0.9967121485104933 1105 82-83 1.0064174758234081 1105 84-85 0.8820454590692677 1105 86-87 0.8247281139448077 1105 88-89 1.0461139806519029 1105 90-91 1.060514658849229 1105 92-93 1.1002749543200352 1105 94-95 1.2667018192784525 1105 96-97 0.8652096953191233 1105 98-99 0.8593691827656329 1105 100-101 0.9739179830919191 1106 1 0.33952502746581104 1106 2 0.3391073140367098 1106 3 0.307183861136064 1106 4 0.12601019602059438 1106 5 0.34471209313717566 1106 6 0.27875845279152855 1106 7 0.42293410413827814 1106 8 0.3573882213995816 1106 9 0.40957604822389015 1106 10-11 0.21043943228714568 1106 12-13 0.3820301308544174 1106 14-15 0.5513473009455225 1106 16-17 0.49331491333313693 1106 18-19 0.43827790847541337 1106 20-21 0.5101525939117906 1106 22-23 0.4256757137985048 1106 24-25 0.6568993950236859 1106 26-27 0.4020795600827327 1106 28-29 0.5380441610491999 1106 30-31 0.4487140331460395 1106 32-33 0.6148682990510608 1106 34-35 0.5246591763699087 1106 36-37 0.5329055828175413 1106 38-39 0.4696791005064185 1106 40-41 0.3731164890353611 1106 42-43 0.06868206188241288 1106 44-45 0.10742221190987067 1106 46-47 0.2672327612340055 1106 48-49 0.3562052921128682 1106 50-51 0.34363459297227195 1106 52-53 0.39068216134177547 1106 54-55 0.5044703146160572 1106 56-57 0.3848533614383918 1106 58-59 0.4803980623033759 1106 60-61 0.49065373504621235 1106 62-63 0.4327538283241772 1106 64-65 0.5154155892096988 1106 66-67 1.0889058727234087 1106 68-69 2.251607047200803 1106 70-71 2.5907741762657217 1106 72-73 1.8174628760489746 1106 74-75 1.3467473392235192 1106 76-77 0.8687683608928616 1106 78-79 0.5923970868151542 1106 80-81 0.5498166527019528 1106 82-83 0.6762313575938101 1106 84-85 0.6444442921202764 1106 86-87 0.45354675906780173 1106 88-89 0.7907153937315776 1106 90-91 0.8549321978319853 1106 92-93 0.7020264636802374 1106 94-95 0.6578812919796633 1106 96-97 0.3451011806453863 1106 98-99 0.7414327713509614 1106 100-101 0.9439624313972388 1107 1 0.14212481382946862 1107 2 0.1714657566371045 1107 3 0.2138788882604139 1107 4 0.08732709148338813 1107 5 0.14646876227605077 1107 6 0.22148128995679173 1107 7 0.03138370135447843 1107 8 0.0631449745003465 1107 9 0.381250574655553 1107 10-11 0.26947338087448713 1107 12-13 0.31495041837721516 1107 14-15 0.31035687726453887 1107 16-17 -0.10402022984519732 1107 18-19 0.18220408265899835 1107 20-21 0.06918129488154534 1107 22-23 0.049568466488778995 1107 24-25 0.30958582538429624 1107 26-27 0.36197726652401485 1107 28-29 0.2690250457275596 1107 30-31 0.20552310849250688 1107 32-33 0.4803257980132045 1107 34-35 0.5063309973631718 1107 36-37 0.3450356257780314 1107 38-39 0.35028413915220824 1107 40-41 0.4096154185213692 1107 42-43 0.2424843223228379 1107 44-45 0.5211035672412123 1107 46-47 0.3379636494107743 1107 48-49 0.4295320017866757 1107 50-51 0.42262056667354386 1107 52-53 0.4686296898305109 1107 54-55 0.1590441381001959 1107 56-57 0.04346843352899654 1107 58-59 0.23059922097734287 1107 60-61 0.4473369841844388 1107 62-63 0.2561586691930877 1107 64-65 0.15976413830174607 1107 66-67 0.3917715383778706 1107 68-69 1.7417998330093774 1107 70-71 2.1595383739866243 1107 72-73 1.2040898474039814 1107 74-75 0.7473312241397672 1107 76-77 0.4621637094046349 1107 78-79 0.32815680190450536 1107 80-81 0.1997451684891196 1107 82-83 0.3692976221763544 1107 84-85 0.16350772065705144 1107 86-87 0.18137965626580765 1107 88-89 0.15792093241701366 1107 90-91 0.3366086833742159 1107 92-93 0.4208748565098901 1107 94-95 0.8287886167208995 1107 96-97 0.42263239813931364 1107 98-99 0.4513514682625619 1107 100-101 0.5335934888971323 1108 1 -0.06558432567839745 1108 2 -0.23702560874230372 1108 3 0.05471273841895652 1108 4 0.13241754317405707 1108 5 0.04055162318746852 1108 6 0.09535774107094852 1108 7 0.016514421736026463 1108 8 -0.14284335544086701 1108 9 -0.11448234172357985 1108 10-11 -0.10887651998242376 1108 12-13 -0.037219373990076576 1108 14-15 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-0.5775260629073671 2316 84-85 -0.881114253558529 2316 86-87 -0.6716912468362572 2316 88-89 -0.6134720007425898 2316 90-91 -0.8037549368033474 2316 92-93 -0.6242691186282947 2316 94-95 -0.6488250297590348 2316 96-97 -0.8587817387786956 2316 98-99 -0.9407137130619461 2316 100-101 -1.2403447045750227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 45.0 5 114.0 6 301.0 7 601.0 8 1219.0 9 2005.0 10 3303.0 11 4879.0 12 6762.0 13 8400.0 14 10180.0 15 12887.0 16 15452.0 17 18246.0 18 15233.0 19 11066.0 20 10245.0 21 10729.0 22 11235.0 23 12244.0 24 13932.0 25 15653.0 26 18157.0 27 21507.0 28 25969.0 29 30543.0 30 35904.0 31 41955.0 32 49679.0 33 59787.0 34 74401.0 35 98016.0 36 105967.0 37 104136.0 38 56654.0 39 4061.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.87264331591116 18.274074911307146 14.376417631689547 18.476864141092143 2 14.813095547720087 24.013515634173615 39.03786155363949 22.13552726446681 3 21.595121774610277 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19.61411069262155 27.441773205965248 30.950740499781126 21.993375601632074 24-25 20.054954699948873 27.913235426417316 32.968815484301324 19.06299438933249 26-27 21.54228795679979 26.19254243387381 33.66837561452456 18.596793994801843 28-29 19.183985588258203 26.231706929068032 35.925512271813176 18.65879521086059 30-31 18.244334062003727 30.29213557109199 34.11664029007847 17.34689007682581 32-33 17.787976266753265 29.99948434606304 32.289481537394785 19.92305784978891 34-35 18.899792801881404 27.556581763164377 32.58106756677435 20.962557868179864 36-37 20.61392983737449 27.673079615936913 33.273239962549184 18.439750584139414 38-39 22.076366811076152 25.474931501584113 34.30087688954047 18.147824797799263 40-41 18.658830170692546 27.11136200394058 35.90627834899827 18.323529476368606 42-43 17.88531585886803 29.538242050192775 33.740065315079086 18.83637677586011 44-45 17.99126167585397 30.621527993221953 31.37148420276382 20.015726128160257 46-47 17.771052253008094 29.056353479525644 29.536352425306962 23.636241842159304 48-49 19.188352958294782 28.429884117096364 30.4156245624535 21.966138362155352 50-51 21.294628738096076 29.219634009206874 29.94693748462593 19.538799768071126 52-53 18.62767702452553 29.02193222266871 32.11490701642282 20.23548373638294 54-55 16.7410802503019 28.70227247776924 32.664727192886154 21.891920079042706 56-57 18.778990805802415 31.088515072612488 28.944464412611225 21.188029708973875 58-59 17.812705907138042 28.862961924632707 30.055570581880886 23.268761586348365 60-61 18.549354235951025 27.54566870126322 30.658403929774053 23.2465731330117 62-63 22.031813740898546 26.980898260650616 29.53454424569771 21.452743752753133 64-65 20.532229281333024 27.181831198319568 31.49636752336026 20.789571996987156 66-67 18.16672378868369 28.23414973877662 31.963338650074917 21.63578782246477 68-69 18.15780287689138 30.12829618569086 30.713397707164035 21.000503230253717 70-71 18.06021134805264 29.891738487752107 28.655030320380305 23.393019843814944 72-73 17.916864902995137 28.7614450576799 27.74673143546542 25.57495860385954 74-75 18.706247084968037 28.506790309747863 29.07426815550495 23.712694449779143 76-77 19.525781748984336 26.99747373319023 30.30713566118614 23.169608856639297 78-79 18.08618430039372 26.888336521544975 31.58754287902369 23.437936299037613 80-81 17.352833076420925 29.939493013993957 29.914588053298814 22.793085856286307 82-83 17.422352803713967 29.285276613765436 28.267753984323274 25.024616598197326 84-85 17.846777011559826 26.905714964362726 28.472734593524457 26.77477343055299 86-87 18.910571835136466 26.901408605229584 29.398037673250542 24.789981886383412 88-89 20.00743848439235 25.325461120205027 30.128165744353115 24.53893465104951 90-91 17.881359976697023 26.570551026554313 31.04946232548138 24.498626671267285 92-93 16.98002043981254 27.797267340789166 30.084869656423535 25.137842562974754 94-95 17.03834959823551 27.98320702371503 28.894052524161314 26.084390853888152 96-97 17.24340552444721 26.624190332601437 27.945060576234106 28.187343566717253 98-99 18.060739600347407 26.135157214864428 28.380425461406656 27.423677723381502 100-101 19.064814113810648 26.274727312426826 28.978232174774604 25.682226398987922 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5234.0 1 3447.0 2 2237.0 3 2859.5 4 2341.0 5 2249.0 6 4412.0 7 8860.5 8 12003.5 9 11736.5 10 9006.5 11 6247.5 12 5307.5 13 4761.0 14 4230.5 15 3819.5 16 3502.0 17 3277.0 18 3172.0 19 3171.5 20 3227.0 21 3347.0 22 3518.5 23 3857.5 24 4189.0 25 4564.5 26 5140.0 27 5767.0 28 6454.0 29 7331.0 30 8287.5 31 8942.0 32 9871.0 33 11287.5 34 12863.0 35 14000.0 36 15696.5 37 17757.5 38 18482.5 39 18533.0 40 18751.5 41 20190.0 42 22082.5 43 23187.0 44 24226.0 45 25885.5 46 30108.0 47 39687.5 48 73958.5 49 74439.0 50 44120.0 51 38527.0 52 30398.0 53 22998.5 54 19672.5 55 17163.5 56 15591.5 57 14038.5 58 12327.5 59 11298.5 60 10437.0 61 9376.0 62 7824.5 63 5790.5 64 4114.0 65 2994.5 66 2216.0 67 1853.0 68 1437.5 69 1016.5 70 771.0 71 574.5 72 488.5 73 470.0 74 370.5 75 267.5 76 188.0 77 97.0 78 47.5 79 38.5 80 35.0 81 21.5 82 8.5 83 5.5 84 4.0 85 1.5 86 1.5 87 0.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020625818038594416 2 0.02611140794247591 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.742794951940747E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.0971179807762988E-4 26-27 2.1942359615525976E-4 28-29 0.0 30-31 1.645676971164448E-4 32-33 0.0016456769711644481 34-35 0.0035656334375229705 36-37 0.10439077587086482 38-39 0.09588811151984851 40-41 0.10455534356798125 42-43 0.10636558823626217 44-45 0.09791777978428466 46-47 0.09347445196214065 48-49 0.09451671404387814 50-51 0.09380358735637354 52-53 0.07383604010624491 54-55 0.0635231310869477 56-57 0.03362666611079356 58-59 0.012671712677966249 60-61 0.00855752025005513 62-63 0.0019199564663585228 64-65 0.0053210222067650486 66-67 0.12583943239504147 68-69 0.3670956763677496 70-71 0.35782502943018984 72-73 0.1503051633663529 74-75 0.027866796711717986 76-77 0.02775708491364036 78-79 0.0053210222067650486 80-81 0.0015359651730868183 82-83 5.485589903881494E-5 84-85 3.839912932717046E-4 86-87 0.0010971179807762988 88-89 3.291353942328896E-4 90-91 8.228384855822241E-4 92-93 0.002468515456746672 94-95 0.06824073840428578 96-97 0.016237346115489222 98-99 0.017828167187614853 100-101 0.009654638230831429 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 911479.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.168748280760994 #Duplication Level Percentage of deduplicated Percentage of total 1 70.37112038013802 21.23008616983514 2 12.148978772428123 7.3303896490738545 3 5.381251635681967 4.870368780969679 4 3.1461089449366257 3.796566752945744 5 1.9324865714386044 2.915035046484105 6 1.3501685318019194 2.443973674352205 7 0.9800048999916486 2.069586479923228 8 0.7010561345485173 1.6919988843102025 9 0.5654505242640913 1.5353041078572933 >10 3.0108305171304033 16.255008747912818 >50 0.24759868124638285 5.142478104914161 >100 0.1394563082755151 7.751944326454701 >500 0.012015817684313219 2.414594060278593 >1k 0.009467007872489204 6.920166599125177 >5k 0.0018205784370171544 4.054092552142385 >10k+ 0.0021846941244205854 9.57840606342078 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19217 2.108331623657813 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16494 1.809586397492427 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16293 1.7875343260788237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12960 1.421864903086083 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11343 1.2444609255945558 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10889 1.1946517692673118 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8394 0.9209208330636252 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7656 0.8399535260823343 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7510 0.8239356035630004 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7054 0.7739070236396011 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6292 0.6903066335044472 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4563 0.500614934628225 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4014 0.4403831574836063 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3853 0.42271955799310795 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3588 0.39364593150253596 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3430 0.3763114674062705 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3366 0.36928991232930214 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3094 0.33944830325218683 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3048 0.33440156054061587 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2898 0.31794479082897137 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2823 0.30971640597314914 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2695 0.29567329581921253 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2415 0.2649539923574762 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2275 0.24959434062660799 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2234 0.24509615690542513 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1964 0.21547397142446506 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1921 0.210756364107127 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1879 0.2061484685878665 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1823 0.20000460789551927 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1813 0.19890748991474297 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1719 0.18859458089544576 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1618 0.17751368928960515 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1428 0.15666844765485546 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 1310 0.14372245548169513 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1093 0.11991499529884946 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1085 0.11903730091422841 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1048 0.11497796438535611 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 951 0.10433591997182602 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 929 0.10192226041411814 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.937030913493344E-4 0.0 18-19 0.0 0.0 0.0 6.034148894269644E-4 0.0 20-21 0.0 0.0 0.0 0.0013165415769315586 0.0 22-23 0.0 0.0 0.0 0.00499188681253216 0.0 24-25 0.0 0.0 0.0 0.013384839365470845 0.0 26-27 0.0 0.0 0.0 0.02764737311556273 0.0 28-29 0.0 0.0 0.0 0.06291971619752074 0.0 30-31 0.0 0.0 0.0 0.15595532096735087 0.0 32-33 0.0 0.0 0.0 0.3058764930404321 0.0 34-35 0.0 0.0 0.0 0.5679779786478898 0.0 36-37 0.0 0.0 0.0 0.9614593424533093 0.0 38-39 0.0 0.0 0.0 1.4321229562063416 0.0 40-41 0.0 0.0 0.0 1.9619212291232162 0.0 42-43 0.0 0.0 0.0 2.430719742308929 0.0 44-45 0.0 0.0 0.0 2.909337461422589 0.0 46-47 0.0 0.0 0.0 3.5053468044793132 0.0 48-49 0.0 0.0 0.0 4.213207325676182 0.0 50-51 0.0 0.0 0.0 4.893585041454603 0.0 52-53 0.0 0.0 0.0 5.453499202943787 0.0 54-55 0.0 0.0 0.0 5.971997160658665 0.0 56-57 0.0 0.0 0.0 6.518087635590069 0.0 58-59 0.0 0.0 0.0 7.237193616089893 0.0 60-61 0.0 0.0 0.0 8.178136852302686 0.0 62-63 0.0 0.0 0.0 8.923518808442104 0.0 64-65 0.0 0.0 0.0 9.481842148859162 0.0 66-67 0.0 0.0 0.0 10.050752677790712 0.0 68-69 0.0 0.0 0.0 10.681046957746695 0.0 70-71 0.0 0.0 0.0 11.485179581756682 0.0 72-73 0.0 0.0 0.0 12.353548463541124 0.0 74-75 0.0 0.0 0.0 13.158119934743421 0.0 76-77 0.0 0.0 0.0 13.879529863002878 0.0 78-79 0.0 0.0 0.0 14.64224628323856 0.0 80-81 0.0 0.0 0.0 15.463877939041932 0.0 82-83 0.0 0.0 0.0 16.30311833843676 0.0 84-85 0.0 0.0 0.0 17.33830400919824 0.0 86-87 0.0 0.0 0.0 18.384022012575166 0.0 88-89 0.0 0.0 0.0 19.19501162396501 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 100 1.2732926E-11 47.468002 1 GTATTAC 65 1.333244E-4 36.513847 1 GATAGTC 80 1.0868371E-5 35.579514 7 ATGGGCG 95 8.7846456E-7 34.955315 5 CGATCGT 70 1.7284037E-8 30.496729 24-25 TACATGG 12695 0.0 26.017063 2 GTACATG 12810 0.0 26.012907 1 ATGGGAG 1865 0.0 25.69102 5 GTGTAAG 130 1.00706075E-5 25.559692 1 ATGGGAT 2420 0.0 25.48395 5 GGTAGCA 200 0.0 25.11508 64-65 GAGTGCA 170 1.1362499E-7 25.114954 9 ACTCGAG 95 1.19362085E-8 24.996878 38-39 ACATGGG 12795 0.0 24.507553 3 CATGGGG 2440 0.0 24.302948 4 TGGGATG 1135 0.0 24.242138 6 CAACCGT 120 1.7253168E-4 23.719677 6 CATTCCG 100 0.0016657234 23.719677 9 ATGGGAC 1310 0.0 23.538612 5 GCTTATA 1940 0.0 23.489319 1 >>END_MODULE