FastQCFastQC Report
Fri 27 May 2016
SRR939823_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939823_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381046
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55880.2346867721161204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44950.18878257706906965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26990.11335354293869164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37100.052.804671
GTATCAA64300.039.6887551
TATCAAC82250.031.950612
ATCAACG82900.031.643543
TCAACGC84300.030.949214
CAACGCA86000.030.3374215
AACGCAG91550.028.705556
ACGCAGA103350.025.2444937
TGGTATC17350.025.1532232
CGCAGAG103500.025.0235238
GTACATG80550.023.9676271
TACATGG81400.023.4847492
GTGGTAT21850.022.5776141
GCAGAGT110000.022.1222849
ACATGGG86150.021.6397253
GAGTACT61600.020.32851612-13
CAGAGTA103600.020.19073510-11
AGAGTAC96200.019.99351310-11
GTACTTT63450.019.700714-15
CATGGGG53050.018.7779584
Produced by FastQC (version 0.11.5)