FastQCFastQC Report
Fri 27 May 2016
SRR939823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381046
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62580.26282566569482485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53300.2238511981708879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37030.15551988495812344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34700.058.3091431
GTATCAA64800.041.5594641
TATCAAC84450.031.4904422
TCAACGC84650.031.41344
ATCAACG84950.031.3037783
CAACGCA86250.030.830665
AACGCAG90450.029.4853366
ACGCAGA103600.025.1060037
CGCAGAG106100.024.3817188
TGGTATC15550.022.7420792
GTACATG85450.021.6362921
GCAGAGT116000.021.5290689
TACATGG85750.021.3352092
GAGTACT62700.020.62940612-13
GTGGTAT19850.020.1960871
ACATGGG87950.020.1573313
GTACTTT64100.020.06857314-15
CAGAGTA108900.019.9471310-11
AGAGTAC102950.019.72687310-11
AGTACTT64500.018.77523212-13
Produced by FastQC (version 0.11.5)