##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939823_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2381046 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.379485738620758 31.0 31.0 34.0 30.0 34.0 2 31.58766021320042 31.0 31.0 34.0 30.0 34.0 3 31.6517333138461 31.0 31.0 34.0 30.0 34.0 4 35.46611405239546 37.0 35.0 37.0 33.0 37.0 5 35.18111661849456 37.0 35.0 37.0 32.0 37.0 6 35.205288348062155 37.0 35.0 37.0 32.0 37.0 7 35.11974401166546 37.0 35.0 37.0 32.0 37.0 8 35.05422406790965 37.0 35.0 37.0 32.0 37.0 9 36.66493549473635 39.0 35.0 39.0 32.0 39.0 10-11 36.50860399169105 38.0 35.0 39.0 32.0 39.0 12-13 36.45099884672535 38.0 35.0 39.0 32.0 39.0 14-15 37.49004135157406 39.0 36.0 41.0 32.0 41.0 16-17 37.406626961427875 39.0 36.0 41.0 32.0 41.0 18-19 37.361848322123976 39.0 36.0 41.0 32.0 41.0 20-21 37.26847423359314 39.0 36.0 40.0 31.0 41.0 22-23 37.12699418658858 39.0 36.0 40.0 31.0 41.0 24-25 36.96846742986065 39.0 36.0 40.0 31.0 41.0 26-27 36.6652026042336 38.5 35.5 40.0 30.0 41.0 28-29 36.4370652645938 38.0 35.0 40.0 30.0 41.0 30-31 36.19383287849122 38.0 35.0 40.0 30.0 41.0 32-33 36.17294940962921 38.0 35.0 40.0 30.0 41.0 34-35 36.28484644983759 38.0 35.0 40.0 30.0 41.0 36-37 36.274411750129985 38.0 35.0 40.0 30.0 41.0 38-39 36.14820356263591 38.0 35.0 40.0 30.0 41.0 40-41 35.97182414787451 38.0 35.0 40.0 29.0 41.0 42-43 35.77856748672642 38.0 34.5 40.0 28.5 41.0 44-45 35.538892570744125 38.0 34.0 40.0 27.5 41.0 46-47 35.250874825601855 38.0 34.0 40.0 27.0 41.0 48-49 34.94557098014906 38.0 33.5 40.0 26.0 41.0 50-51 34.665125537263876 38.0 33.0 40.0 26.0 41.0 52-53 34.360503115017515 37.5 33.0 40.0 24.5 41.0 54-55 34.031836428191646 37.0 33.0 40.0 24.0 41.0 56-57 32.77312030090977 36.5 31.0 39.5 17.0 40.5 58-59 29.400430315080012 34.0 24.5 38.5 8.0 40.0 60-61 29.364046725682748 34.0 24.5 38.0 9.0 40.0 62-63 31.143451869472493 34.5 29.0 38.0 12.5 40.0 64-65 30.64711895528268 35.0 29.0 38.0 4.5 40.0 66-67 25.183237115116633 28.5 18.0 35.5 2.0 39.0 68-69 17.441860216056305 13.0 7.0 29.0 2.0 35.5 70-71 16.270443956143644 13.5 7.5 25.5 2.0 33.0 72-73 18.396566467006515 18.5 7.0 30.0 2.0 34.0 74-75 19.129744448448285 18.0 7.5 30.5 2.0 35.0 76-77 20.174358034242093 23.0 8.5 31.0 2.0 34.5 78-79 23.30567994066473 28.0 12.0 33.5 2.0 35.5 80-81 25.9961317420999 31.5 22.0 34.5 2.0 36.0 82-83 26.915470973681316 32.5 26.0 35.0 2.0 36.0 84-85 26.898509730597393 33.0 26.0 35.0 2.0 36.0 86-87 26.60560757751005 33.0 25.0 35.0 2.0 35.0 88-89 26.177913404444936 33.0 24.5 35.0 2.0 35.0 90-91 24.60650067239356 31.5 11.5 34.0 2.0 35.0 92-93 22.541850514437773 30.5 2.0 34.0 2.0 35.0 94-95 18.058670643070315 23.0 2.0 33.0 2.0 35.0 96-97 16.711440476160476 14.5 2.0 32.5 2.0 34.5 98-99 15.596564073100646 2.0 2.0 32.0 2.0 34.0 100-101 14.956526879363103 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3208056061189417 1101 2 0.3150193071413767 1101 3 0.3394282841007872 1101 4 0.21667793293760695 1101 5 0.3751956871830373 1101 6 0.3760496973138103 1101 7 0.3947617687498095 1101 8 0.37215420516964315 1101 9 0.403113890611003 1101 10-11 0.4020590373025499 1101 12-13 0.5027790407091501 1101 14-15 0.4191692727213834 1101 16-17 0.48892164524579584 1101 18-19 0.4631527265497084 1101 20-21 0.4875402340914121 1101 22-23 0.43299640293590613 1101 24-25 0.6181388367158718 1101 26-27 0.6514169554385631 1101 28-29 0.4269077447931764 1101 30-31 0.4922026819228975 1101 32-33 0.3998334135226287 1101 34-35 0.6858486694416612 1101 36-37 0.5730176980405375 1101 38-39 0.45490121168825937 1101 40-41 0.3850112901733169 1101 42-43 0.47280116425589114 1101 44-45 0.3426462666036727 1101 46-47 0.16880953387788367 1101 48-49 0.25161028490848025 1101 50-51 0.2831346814203499 1101 52-53 0.3180045980747721 1101 54-55 0.11849587172371656 1101 56-57 1.018203138185335 1101 58-59 -8.24510611032538 1101 60-61 -9.659414695491382 1101 62-63 -2.7676234706786893 1101 64-65 -0.005945690933941705 1101 66-67 -1.3128507989288636 1101 68-69 -3.716991293392981 1101 70-71 -4.440314883927744 1101 72-73 -7.128894364850726 1101 74-75 -7.757914616039297 1101 76-77 -8.50910852337541 1101 78-79 -11.630922930076336 1101 80-81 -7.7571667942114075 1101 82-83 -4.127267633537063 1101 84-85 -3.290659846081766 1101 86-87 -3.1015442836978266 1101 88-89 -3.2836538712608103 1101 90-91 -9.125879704127277 1101 92-93 -14.722807745485108 1101 94-95 -12.683566960635005 1101 96-97 -12.275077998539068 1101 98-99 -11.776287402913209 1101 100-101 -11.561665143368618 1102 1 0.3412958344896069 1102 2 0.3838461626530112 1102 3 0.35818926345958957 1102 4 0.31151214461964116 1102 5 0.4136097188035919 1102 6 0.4142304175597502 1102 7 0.2814822342746197 1102 8 0.33400093332072345 1102 9 0.5178481901279142 1102 10-11 0.5169099925067542 1102 12-13 0.45761956552249927 1102 14-15 0.5120958208109769 1102 16-17 0.5823285402831289 1102 18-19 0.6266422412620472 1102 20-21 0.7157118963663436 1102 22-23 0.6018795276614242 1102 24-25 0.6298044054466558 1102 26-27 0.7543347200411077 1102 28-29 0.6106404523030591 1102 30-31 0.7198253762575462 1102 32-33 0.5609966765880614 1102 34-35 0.521528840719661 1102 36-37 0.46631891674936554 1102 38-39 0.5211959525753898 1102 40-41 0.6228859607925514 1102 42-43 0.6262514277605007 1102 44-45 0.33885838781579025 1102 46-47 0.4735141891260213 1102 48-49 0.4541039717771582 1102 50-51 0.440729652874019 1102 52-53 0.2894170925850972 1102 54-55 0.34455400742858444 1102 56-57 0.8743117789407151 1102 58-59 -9.15450581388269 1102 60-61 -9.878912664310004 1102 62-63 -3.1831990635640643 1102 64-65 -0.2768888178553297 1102 66-67 -1.3979408296710716 1102 68-69 -3.681136824793949 1102 70-71 -4.095912984498671 1102 72-73 -7.0234993824177 1102 74-75 -7.735496137778426 1102 76-77 -8.71604198846105 1102 78-79 -11.759511807985865 1102 80-81 -8.057589499525417 1102 82-83 -4.3067458395098335 1102 84-85 -3.3724217586860696 1102 86-87 -3.2078862358278215 1102 88-89 -3.506974029363576 1102 90-91 -8.976155560517263 1102 92-93 -14.844390420056913 1102 94-95 -12.598456343595041 1102 96-97 -12.3832109585742 1102 98-99 -11.80121054740157 1102 100-101 -11.394586750746747 1103 1 0.2081295639736247 1103 2 0.3260070779056825 1103 3 0.2849443989604765 1103 4 0.25478596860151015 1103 5 0.26482874965129355 1103 6 0.22124647756646 1103 7 0.23794469538245977 1103 8 0.32606129280578244 1103 9 0.32464665641751367 1103 10-11 0.33597521423547505 1103 12-13 0.3360448593101708 1103 14-15 0.4579413712001852 1103 16-17 0.3977606336937285 1103 18-19 0.5408898947198395 1103 20-21 0.4902506482892619 1103 22-23 0.39390638060566374 1103 24-25 0.4246496753496132 1103 26-27 0.42746630568483823 1103 28-29 0.5167240039530085 1103 30-31 0.4269917638837697 1103 32-33 0.4147183966338588 1103 34-35 0.4390561230681129 1103 36-37 0.2941959611182412 1103 38-39 0.39491840275293555 1103 40-41 0.4884902134846456 1103 42-43 0.5236140617866667 1103 44-45 0.5508828624100985 1103 46-47 0.4950588587305589 1103 48-49 0.5509008259937076 1103 50-51 0.5769935615222508 1103 52-53 0.6120803818502765 1103 54-55 0.44440837867671945 1103 56-57 1.1961666822452557 1103 58-59 -6.043461270063222 1103 60-61 -8.140065426665856 1103 62-63 -2.174196936784277 1103 64-65 0.1058823863270959 1103 66-67 -1.4622820628852722 1103 68-69 -3.7986065279411765 1103 70-71 -4.575611888288995 1103 72-73 -7.505605913699146 1103 74-75 -7.897014110585719 1103 76-77 -8.845102894460563 1103 78-79 -11.892131383323894 1103 80-81 -8.342232081965243 1103 82-83 -4.462462156580745 1103 84-85 -3.4796837140511627 1103 86-87 -3.1179385233811736 1103 88-89 -3.2426764594733406 1103 90-91 -8.79981302174076 1103 92-93 -14.829830436481263 1103 94-95 -12.621513867855656 1103 96-97 -12.445656740870604 1103 98-99 -12.044546107275266 1103 100-101 -11.765608255656701 1104 1 0.039210379851585486 1104 2 0.03641357771597242 1104 3 0.11622582626717914 1104 4 0.03512735691981561 1104 5 -0.1136225301187892 1104 6 -0.04056670085996217 1104 7 0.017799819041961484 1104 8 0.19848986859547324 1104 9 0.18176988852929554 1104 10-11 0.11375444571926607 1104 12-13 0.03331567165562177 1104 14-15 0.19087195528472733 1104 16-17 0.24079636433362595 1104 18-19 0.16494080641155762 1104 20-21 0.05777492881381363 1104 22-23 0.13664293854966303 1104 24-25 0.17578792715184477 1104 26-27 0.06403035524458289 1104 28-29 0.12337623531904285 1104 30-31 0.19528665396178724 1104 32-33 0.18620562509975258 1104 34-35 0.22409116465137657 1104 36-37 0.12947217252779808 1104 38-39 0.18234987868610375 1104 40-41 0.23821546304962027 1104 42-43 0.17514219753954308 1104 44-45 -0.0036474669851500607 1104 46-47 0.06634460354410265 1104 48-49 0.12790617708551366 1104 50-51 0.15045986534389755 1104 52-53 0.16476922916316994 1104 54-55 0.060575479274625366 1104 56-57 0.7513402765478858 1104 58-59 -6.7432299127524615 1104 60-61 -8.987040337153907 1104 62-63 -2.829070050055595 1104 64-65 -0.5282634232926604 1104 66-67 -1.9296925182296008 1104 68-69 -4.3088791414283705 1104 70-71 -5.020408856439749 1104 72-73 -7.907040285414681 1104 74-75 -8.607738972011193 1104 76-77 -9.174779430337301 1104 78-79 -12.006129917988241 1104 80-81 -8.719687936548127 1104 82-83 -5.303393647777828 1104 84-85 -4.5032282999412026 1104 86-87 -4.280922225883145 1104 88-89 -4.521012823783696 1104 90-91 -10.023351938177377 1104 92-93 -15.337612851581197 1104 94-95 -12.579729996142305 1104 96-97 -12.29230456750744 1104 98-99 -11.640031695960781 1104 100-101 -11.081884166149116 1105 1 0.1301114108435968 1105 2 0.10439717767107837 1105 3 0.01965407198364133 1105 4 0.04139637185761558 1105 5 0.13511541405302552 1105 6 0.05877454954128325 1105 7 0.10626539746547792 1105 8 0.10994364812924573 1105 9 0.17620983190066397 1105 10-11 0.18626130972471344 1105 12-13 0.20742467140190257 1105 14-15 0.02857764358263637 1105 16-17 0.306164756991528 1105 18-19 0.21976104696693 1105 20-21 0.15971417344467653 1105 22-23 0.2160901271291138 1105 24-25 0.10701063972379643 1105 26-27 0.07178213054849891 1105 28-29 0.2570001000818678 1105 30-31 0.24102688346307133 1105 32-33 0.18912610890170356 1105 34-35 0.31068696378257954 1105 36-37 0.1038912747256262 1105 38-39 0.3011544712268588 1105 40-41 0.3462385445942928 1105 42-43 0.32040044542846857 1105 44-45 0.46387516859173417 1105 46-47 0.1942196967225982 1105 48-49 0.27780723731616064 1105 50-51 0.2492392937946022 1105 52-53 0.17209028478480093 1105 54-55 0.1822883728526037 1105 56-57 0.8171273633202958 1105 58-59 -6.417317297916146 1105 60-61 -8.804614046024504 1105 62-63 -2.7442047537063097 1105 64-65 -0.2287382052840492 1105 66-67 -1.5814845793427494 1105 68-69 -3.773447417257483 1105 70-71 -4.600660028893309 1105 72-73 -6.072187800617984 1105 74-75 -6.551024429437067 1105 76-77 -8.399749350698176 1105 78-79 -11.720104618080013 1105 80-81 -7.962439034333123 1105 82-83 -4.006970318868021 1105 84-85 -3.112156757401973 1105 86-87 -2.8465216194801357 1105 88-89 -2.9566835030600735 1105 90-91 -8.31185379766027 1105 92-93 -11.675052326820005 1105 94-95 -11.576580869355722 1105 96-97 -12.131343914850795 1105 98-99 -11.59918131383068 1105 100-101 -11.112039888605928 1106 1 0.12515078201584373 1106 2 0.1376738444003749 1106 3 0.11647537775538197 1106 4 0.11262702835424676 1106 5 0.16400492561194824 1106 6 -0.010046047149074866 1106 7 0.1304864086580224 1106 8 0.20018732541878848 1106 9 0.09250424445369276 1106 10-11 0.21857818414623864 1106 12-13 0.18659744790483757 1106 14-15 0.17429174490775523 1106 16-17 0.14375701722112666 1106 18-19 0.23706451174444965 1106 20-21 0.16330638956091548 1106 22-23 0.12966388915715044 1106 24-25 0.16637986771551994 1106 26-27 0.2203454646687959 1106 28-29 0.30949810068474903 1106 30-31 0.4000884616266305 1106 32-33 0.3062349030630571 1106 34-35 0.2443940625205201 1106 36-37 0.30288124220428614 1106 38-39 0.3845744655247998 1106 40-41 0.49843559406415494 1106 42-43 0.5039870084995428 1106 44-45 0.48750612277184047 1106 46-47 0.38111002356174595 1106 48-49 0.4335051719123868 1106 50-51 0.4879139651296569 1106 52-53 0.3915968850418068 1106 54-55 0.27778693140776767 1106 56-57 1.2044682999449279 1106 58-59 -6.575512865503942 1106 60-61 -8.828380853009772 1106 62-63 -2.389462935304664 1106 64-65 -0.1884095280884459 1106 66-67 -1.3949158715618921 1106 68-69 -4.016151762178197 1106 70-71 -4.453043334764715 1106 72-73 -5.846663076306051 1106 74-75 -6.548195211035916 1106 76-77 -8.505296442116574 1106 78-79 -11.953127224542293 1106 80-81 -7.9133041054577475 1106 82-83 -3.758415317270952 1106 84-85 -2.819319541412355 1106 86-87 -2.5990734547183756 1106 88-89 -2.592143944406054 1106 90-91 -8.098918535647346 1106 92-93 -11.515034142313432 1106 94-95 -11.611278149603802 1106 96-97 -11.888553140424452 1106 98-99 -11.430568294998361 1106 100-101 -11.155453559989184 1107 1 0.0343118315395472 1107 2 0.04626553354107443 1107 3 0.06445418935690128 1107 4 0.04653649431878648 1107 5 0.03875678279178629 1107 6 -0.04014169969342163 1107 7 0.060558467563474494 1107 8 0.027690790734503423 1107 9 0.028428017338697487 1107 10-11 -0.022495473883630268 1107 12-13 0.09269271705255733 1107 14-15 0.03785104194839306 1107 16-17 0.1481736972684473 1107 18-19 0.08096350803172925 1107 20-21 0.22524288662646086 1107 22-23 0.093394813536257 1107 24-25 0.1883563592758719 1107 26-27 0.1707423784897557 1107 28-29 0.191456503788622 1107 30-31 0.2126905778449668 1107 32-33 0.16844338235472378 1107 34-35 0.13015644836172413 1107 36-37 0.016217874277863586 1107 38-39 0.0357490682832875 1107 40-41 -0.04265797109852798 1107 42-43 0.18913555011773298 1107 44-45 0.14694939481632474 1107 46-47 -0.019884759622549097 1107 48-49 0.04875428141636462 1107 50-51 -0.013991466978012568 1107 52-53 -0.05633400198070859 1107 54-55 -0.24472590582561082 1107 56-57 0.2583086562088681 1107 58-59 -6.911745239908772 1107 60-61 -9.14216781228125 1107 62-63 -3.0839985502302625 1107 64-65 -0.7940682329942881 1107 66-67 -2.1109509441614804 1107 68-69 -4.378721682910125 1107 70-71 -5.006396744537067 1107 72-73 -6.661154567850026 1107 74-75 -7.296417577805585 1107 76-77 -9.424659938169556 1107 78-79 -12.768610386220981 1107 80-81 -8.99127534743345 1107 82-83 -5.111500103633826 1107 84-85 -4.246223662762304 1107 86-87 -3.979964491429424 1107 88-89 -4.168536624479586 1107 90-91 -9.160877807128205 1107 92-93 -12.633169949920415 1107 94-95 -12.05954401714518 1107 96-97 -12.360154837200316 1107 98-99 -11.698884741403116 1107 100-101 -11.120400212150132 1108 1 -0.049904634361173095 1108 2 -0.021378140134732604 1108 3 -0.030449890955214443 1108 4 0.01411094506727295 1108 5 -0.1461289609721561 1108 6 -0.05741148353230585 1108 7 0.010730557051232381 1108 8 0.04224158524656474 1108 9 -0.01514294553285822 1108 10-11 0.14128180895209397 1108 12-13 -0.0861066784066935 1108 14-15 -0.0652275194682872 1108 16-17 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2312 80-81 3.0934864369128903 2312 82-83 1.7017120745227636 2312 84-85 1.2998174416321682 2312 86-87 1.1856779010577405 2312 88-89 1.364787141238068 2312 90-91 2.987826909486607 2312 92-93 4.821357010382169 2312 94-95 8.727916194357054 2312 96-97 9.25465834861197 2312 98-99 9.567249973821495 2312 100-101 8.840515977933423 2313 1 -0.17426500072123474 2313 2 -0.2712903161031974 2313 3 -0.15067047081329576 2313 4 -0.24662864441252452 2313 5 -0.3004395238022326 2313 6 -0.2340716373113807 2313 7 -0.2444173710495079 2313 8 -0.31549753271637826 2313 9 -0.31630605303716663 2313 10-11 -0.43667839211845205 2313 12-13 -0.3250652790039581 2313 14-15 -0.3500227856796059 2313 16-17 -0.31959071095123903 2313 18-19 -0.4408299057267513 2313 20-21 -0.48985186495862365 2313 22-23 -0.43514755037893593 2313 24-25 -0.37718930690334673 2313 26-27 -0.4715363232134493 2313 28-29 -0.519838521619576 2313 30-31 -0.5860721418384145 2313 32-33 -0.49677809851060317 2313 34-35 -0.46925389980092547 2313 36-37 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4.122923639729915 2314 60-61 3.8992057240323277 2314 62-63 1.4916485377265296 2314 64-65 1.561855496119069 2314 66-67 2.0889998240896404 2314 68-69 -0.33333354805640525 2314 70-71 2.3852500722029077 2314 72-73 8.36810943224431 2314 74-75 10.181197306101822 2314 76-77 8.956893190847104 2314 78-79 6.656760473300498 2314 80-81 3.7257762309305207 2314 82-83 2.17043567693997 2314 84-85 1.640948666046821 2314 86-87 1.6941180881962978 2314 88-89 1.8754812758305448 2314 90-91 3.122512521044147 2314 92-93 5.338211663937585 2314 94-95 9.14705863842125 2314 96-97 9.884021665989431 2314 98-99 10.193414174908808 2314 100-101 9.432977911265313 2315 1 -0.6928728450595081 2315 2 -0.5460216580662554 2315 3 -0.5045321536096523 2315 4 -0.40344692677058447 2315 5 -0.6448309682602869 2315 6 -0.7241887987580213 2315 7 -0.6840471538091109 2315 8 -0.8363895658741214 2315 9 -0.8792935167964018 2315 10-11 -0.8388921411486336 2315 12-13 -0.55845833302822 2315 14-15 -0.5986127138106028 2315 16-17 -0.8989852009943817 2315 18-19 -0.8354233116096594 2315 20-21 -0.8532461014865831 2315 22-23 -0.857267345661171 2315 24-25 -0.968298452846426 2315 26-27 -0.8151935945053168 2315 28-29 -0.7477029454425121 2315 30-31 -0.8471281261579122 2315 32-33 -0.7819146223920797 2315 34-35 -0.9291555393125464 2315 36-37 -1.0450203210391535 2315 38-39 -0.8596330195126356 2315 40-41 -0.7873898041027729 2315 42-43 -0.6160912319281735 2315 44-45 -0.5689348220607471 2315 46-47 -0.7051207410940563 2315 48-49 -0.6467833739018545 2315 50-51 -0.5566871479848103 2315 52-53 -0.37326574423723713 2315 54-55 -0.3704386120024381 2315 56-57 0.8381386039541425 2315 58-59 4.305328794276189 2315 60-61 3.925040972297616 2315 62-63 1.3145699917231077 2315 64-65 1.3603562170346315 2315 66-67 2.081158740914006 2315 68-69 5.929034941174919 2315 70-71 5.113259049854523 2315 72-73 9.306764305182945 2315 74-75 10.240349272849592 2315 76-77 8.985472705580818 2315 78-79 6.456671766893365 2315 80-81 3.614168082331801 2315 82-83 1.8658756043978428 2315 84-85 1.2501124603155382 2315 86-87 1.2888176691641284 2315 88-89 1.4256615047212335 2315 90-91 2.735020969273396 2315 92-93 4.610806171462141 2315 94-95 8.647819823876777 2315 96-97 9.56232379719606 2315 98-99 9.836437063977924 2315 100-101 9.029208874588731 2316 1 -0.5074162677925855 2316 2 -0.4817762344817531 2316 3 -0.5640069301102919 2316 4 -0.41899343592341864 2316 5 -0.6165449955059188 2316 6 -0.6193090627001183 2316 7 -0.5861464513825467 2316 8 -0.7323346488881768 2316 9 -0.708667718584401 2316 10-11 -0.5374393952993373 2316 12-13 -0.5928931989285999 2316 14-15 -0.9024042208348604 2316 16-17 -0.9426527190871852 2316 18-19 -0.827438530851893 2316 20-21 -0.7469708247730864 2316 22-23 -0.9747535099864209 2316 24-25 -2.073850017341954 2316 26-27 -1.4976621981664522 2316 28-29 -1.1064473579584728 2316 30-31 -0.6281626089165258 2316 32-33 -0.6750925704550852 2316 34-35 -0.6382631382908315 2316 36-37 -0.6121965130357481 2316 38-39 -0.9530185026969704 2316 40-41 -0.7058803852011195 2316 42-43 -0.8897249590460987 2316 44-45 -0.7197623018393813 2316 46-47 -0.7296404835376507 2316 48-49 -0.8861139401002305 2316 50-51 -0.8889961092449212 2316 52-53 -0.633713009030771 2316 54-55 -0.6418772561063122 2316 56-57 0.5166161475897297 2316 58-59 4.101859232760656 2316 60-61 3.9261717726381846 2316 62-63 1.427406571118592 2316 64-65 1.1076689032841003 2316 66-67 6.096362018904642 2316 68-69 8.71471368744993 2316 70-71 5.75550974163826 2316 72-73 9.299392817315763 2316 74-75 10.18528461767718 2316 76-77 8.913916990382859 2316 78-79 6.536065923981482 2316 80-81 3.4980774586621557 2316 82-83 1.9722360237252197 2316 84-85 1.6252601295364855 2316 86-87 1.5524591123314302 2316 88-89 1.5628368985058216 2316 90-91 2.9843584533091594 2316 92-93 5.053300317907439 2316 94-95 9.048198974579204 2316 96-97 9.745095721419135 2316 98-99 9.94027912442918 2316 100-101 8.608125434827135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 38.0 5 90.0 6 255.0 7 580.0 8 1372.0 9 2462.0 10 3810.0 11 5560.0 12 7749.0 13 10724.0 14 14503.0 15 20212.0 16 27562.0 17 35873.0 18 39893.0 19 41645.0 20 43424.0 21 42850.0 22 41747.0 23 40009.0 24 41367.0 25 45010.0 26 59864.0 27 86607.0 28 119613.0 29 155518.0 30 183519.0 31 197869.0 32 203837.0 33 210915.0 34 211675.0 35 200061.0 36 162433.0 37 94720.0 38 26659.0 39 1005.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.56433414267351 18.930284725314426 14.428028934611476 24.07735219740059 2 16.468703641791645 20.333182691031517 38.27211879797524 24.925994869201602 3 19.869758963783667 23.639441068136097 29.395456473622094 27.09534349445814 4 14.02522252825019 16.700097352172115 35.2391343972355 34.0355457223422 5 14.408415432776096 36.81617359372616 33.69484684599877 15.080564127498967 6 33.517468170488165 35.350192877497065 16.550086201646756 14.58225275036801 7 29.52336914112537 31.744661799898026 20.00200752106427 18.729961537912327 8 27.006148146001564 35.030614734803194 18.8697558170936 19.09348130210164 9 26.479328832790294 16.23147977821512 19.11416243113321 38.17502895786138 10-11 25.74492470956042 25.898155684518485 26.641190468390786 21.715729137530314 12-13 26.3659752898516 23.03813114068355 27.11129898372396 23.48459458574089 14-15 23.129383981498844 24.493955478707733 25.006609505382205 27.37005103441122 16-17 23.125487705823407 27.812650406586016 25.72879734368845 23.333064543902136 18-19 23.18312854680523 26.384388009152392 27.67948640412328 22.752997039919094 20-21 23.746685280334777 25.62938725249323 27.807484609705146 22.816442857466846 22-23 23.4933722406035 25.400265261569917 27.805888672457403 23.300473825369185 24-25 23.3168321821586 25.73589506460606 27.76221038988747 23.18506236334787 26-27 23.063643510997696 25.987319007964572 27.71252862189863 23.2365088591391 28-29 23.037427630045425 26.080441160103234 27.75054126531135 23.131589944539993 30-31 23.144062526841605 25.9292471582288 27.669937676025796 23.256752638903794 32-33 22.94984062639842 25.943804819783622 27.718218148746317 23.38813640507165 34-35 23.299196124699893 25.993074001424176 27.62383986583949 23.08389000803644 36-37 23.408098649384932 25.763514867435898 27.601407452558757 23.226979030620413 38-39 23.08345370372899 25.958396546607133 27.69805244655586 23.260097303108022 40-41 23.353620805469216 25.78162709503367 27.727245803421287 23.137506296075827 42-43 23.139379327858283 25.842603170722334 27.618706745047284 23.399310756372103 44-45 23.051118026316757 25.982089313976825 27.614803674557777 23.351988985148644 46-47 23.26257906056379 26.136916691050082 27.288038079722334 23.312466168663796 48-49 23.218304418567232 26.25757912473896 27.184214163495263 23.33990229319854 50-51 23.126103237395263 26.432475780237212 27.14659226194227 23.294828720425258 52-53 23.291103986772928 26.292231446895958 27.048310238043634 23.368354328287488 54-55 23.13828731527055 26.340316989596445 26.998974031551942 23.52242166358106 56-57 22.99181902632133 26.304294196217604 27.305658498031367 23.398228279429706 58-59 21.48126094259636 25.99704854079671 28.956569874548173 23.56512064205876 60-61 22.903218263981444 26.149013620552775 27.511679769873936 23.436088345591852 62-63 22.856416282660152 26.300190337315353 27.303661095422328 23.539732284602167 64-65 22.210032214845832 26.219843663681132 27.798684446552986 23.771439674920053 66-67 22.178052099728056 25.80120207202676 28.603239336387396 23.417506491857782 68-69 22.638709946183003 25.053082039135983 29.406640257133564 22.90156775754745 70-71 21.769056265434443 25.39162856766207 29.46063231680165 23.378682850101836 72-73 21.625712856471026 25.53340941700141 29.24807255420747 23.592805172320094 74-75 22.13977894117542 25.73031028187624 28.49800396859765 23.631906808350696 76-77 20.703244874845154 26.044054319176453 28.955184322083845 24.297516483894555 78-79 21.72122539289959 26.264528912736683 27.75057128709781 24.26367440726591 80-81 22.327948642620736 26.328195593481084 26.96212013193392 24.38173563196426 82-83 22.438910687230486 26.2745415086039 26.753302387504636 24.53324541666098 84-85 22.45073066657707 26.173318857896426 26.700125365123295 24.675825110403203 86-87 22.355552316918974 26.133249222114724 26.830652743236016 24.680545717730283 88-89 22.28419778534308 26.17454681681916 26.701227107750125 24.840028290087634 90-91 21.982380690401815 26.157695725324317 26.924252290598645 24.935671293675224 92-93 21.677045536251647 26.027609062275108 27.075628417185747 25.219716984287494 94-95 20.375917791730284 25.824621762266492 28.55951506940998 25.23994537659324 96-97 20.125218438878804 25.69192163255305 28.79258388308181 25.39027604548633 98-99 20.45576799888004 25.78450130025496 28.128311619994655 25.631419080870344 100-101 20.450034472071295 25.893438077828872 27.960855750721592 25.695671699378238 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 213.0 1 157.5 2 105.5 3 133.5 4 163.0 5 192.0 6 275.0 7 444.0 8 696.0 9 940.0 10 1065.5 11 1230.5 12 1421.0 13 1606.5 14 1792.0 15 1948.0 16 2152.5 17 2394.5 18 2700.5 19 3087.5 20 3462.0 21 3871.0 22 4583.5 23 5639.0 24 6816.5 25 8218.0 26 10200.5 27 12616.5 28 15034.5 29 17708.0 30 20807.0 31 24422.5 32 28332.5 33 31771.0 34 35229.0 35 39513.0 36 44068.5 37 48486.0 38 52884.5 39 57433.0 40 62678.0 41 68218.5 42 73742.0 43 79160.5 44 83826.5 45 88133.5 46 92713.5 47 96656.5 48 99581.0 49 101582.0 50 102092.0 51 101673.5 52 100176.0 53 97774.5 54 94473.5 55 88362.0 56 80693.0 57 72689.5 58 63887.5 59 54666.0 60 45363.5 61 36580.5 62 28380.0 63 21205.5 64 15590.0 65 11346.0 66 8047.0 67 5637.5 68 3974.5 69 2709.5 70 1841.0 71 1279.0 72 916.0 73 623.5 74 409.0 75 256.5 76 147.0 77 99.5 78 62.5 79 36.0 80 20.5 81 13.0 82 9.5 83 4.5 84 0.5 85 2.0 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02125116440421563 2 0.005459785321241168 3 0.003191874495494837 4 0.0 5 0.06354350146952222 6 4.199834862493207E-5 7 0.0 8 1.259950458747962E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.3099091743712636E-4 16-17 0.0 18-19 0.001259950458747962 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.399669724986414E-5 28-29 0.0021419157798715353 30-31 0.007580701926800239 32-33 2.3099091743712636E-4 34-35 7.349711009363111E-4 36-37 0.02414905045933594 38-39 0.022952097523525377 40-41 0.024989017431834577 42-43 0.025639991835521028 44-45 0.026185970367645146 46-47 0.028201891101641886 48-49 0.027676911743830232 50-51 0.023183088440962504 52-53 0.0199072172482178 54-55 0.015476391468287467 56-57 0.15285298982044027 58-59 0.7621440324966422 60-61 0.31038879551256043 62-63 4.1998348624932067E-4 64-65 0.14344535972845548 66-67 1.4146093775592743 68-69 2.712442346766925 70-71 2.1942037239095757 72-73 1.8540171000476262 74-75 1.5600286596731017 76-77 1.1214609041572485 78-79 0.4235113475338149 80-81 2.3099091743712636E-4 82-83 1.4699422018726223E-4 84-85 4.199834862493207E-5 86-87 0.0030028819266826427 88-89 0.0 90-91 0.2249851535837611 92-93 0.17561609477515344 94-95 1.2923521847120971 96-97 1.2714580062711933 98-99 1.000232670851382 100-101 0.4015462112029755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2381046.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.21652200924951 #Duplication Level Percentage of deduplicated Percentage of total 1 73.24055131327906 38.97607410932683 2 14.330408233149164 15.252289702818258 3 5.149146708399217 8.220590374091447 4 2.339434413219507 4.979862517611664 5 1.3221657870701182 3.5180532353746825 6 0.8374642905645321 2.674016211047277 7 0.5436277358710762 2.0250984159575123 8 0.38218249740477517 1.627073862775293 9 0.2693952006441485 1.2902648061838966 >10 1.4636117663011243 13.921076612180821 >50 0.0892381606066484 3.212815461628486 >100 0.030876413174602838 3.0149033390117554 >500 0.0015812335966662592 0.5805774183649034 >1k 1.581233596663516E-4 0.21975185565011685 >5k 1.581233596663516E-4 0.4875520779769393 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6258 0.26282566569482485 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5330 0.2238511981708879 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3703 0.15551988495812344 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.199834862493207E-5 2 0.0 0.0 0.0 0.0 4.199834862493207E-5 3 0.0 0.0 0.0 0.0 4.199834862493207E-5 4 0.0 0.0 0.0 0.0 4.199834862493207E-5 5 0.0 0.0 0.0 0.0 4.199834862493207E-5 6 0.0 0.0 0.0 0.0 4.199834862493207E-5 7 0.0 0.0 0.0 0.0 4.199834862493207E-5 8 0.0 0.0 0.0 4.199834862493207E-5 4.199834862493207E-5 9 0.0 0.0 0.0 8.399669724986414E-5 4.199834862493207E-5 10-11 0.0 0.0 0.0 8.399669724986414E-5 4.199834862493207E-5 12-13 0.0 0.0 0.0 8.399669724986414E-5 1.4699422018726226E-4 14-15 0.0 0.0 0.0 1.259950458747962E-4 1.6799339449972828E-4 16-17 0.0 0.0 0.0 1.4699422018726226E-4 1.6799339449972828E-4 18-19 0.0 0.0 0.0 1.6799339449972828E-4 2.0999174312466033E-4 20-21 0.0 0.0 0.0 2.519900917495924E-4 2.3099091743712636E-4 22-23 0.0 0.0 0.0 3.989843119368546E-4 2.9398844037452446E-4 24-25 0.0 0.0 0.0 6.50974403686447E-4 2.9398844037452446E-4 26-27 0.0 0.0 0.0 0.0017639306422471469 2.9398844037452446E-4 28-29 0.0 0.0 0.0 0.0063627498166772085 2.9398844037452446E-4 30-31 0.0 0.0 0.0 0.02282610247765058 2.9398844037452446E-4 32-33 0.0 0.0 0.0 0.05818871201984338 2.9398844037452446E-4 34-35 0.0 0.0 0.0 0.11135862137900737 2.9398844037452446E-4 36-37 0.0 0.0 0.0 0.2008571022987376 2.9398844037452446E-4 38-39 0.0 0.0 0.0 0.3368267559719552 2.9398844037452446E-4 40-41 0.0 0.0 0.0 0.48579489854458924 2.9398844037452446E-4 42-43 0.0 0.0 0.0 0.6340910675392244 2.9398844037452446E-4 44-45 0.0 0.0 0.0 0.8102531408465019 2.9398844037452446E-4 46-47 0.0 0.0 0.0 1.0104802679158655 2.9398844037452446E-4 48-49 0.0 0.0 0.0 1.2116523578292901 2.9398844037452446E-4 50-51 0.0 0.0 0.0 1.4206151414126396 2.9398844037452446E-4 52-53 0.0 0.0 0.0 1.620422284995754 2.9398844037452446E-4 54-55 0.0 0.0 0.0 1.83625179857928 2.9398844037452446E-4 56-57 0.0 0.0 0.0 2.023207447483165 2.9398844037452446E-4 58-59 0.0 0.0 0.0 2.1555232448260133 3.3598678899945657E-4 60-61 0.0 0.0 0.0 2.2545763500579157 3.3598678899945657E-4 62-63 4.199834862493207E-5 0.0 0.0 2.340462972995902 3.3598678899945657E-4 64-65 6.29975229373981E-5 8.399669724986414E-5 0.0 2.4301504464844443 3.3598678899945657E-4 66-67 8.399669724986414E-5 8.399669724986414E-5 0.0 2.504592519422136 3.3598678899945657E-4 68-69 1.0499587156233018E-4 8.399669724986414E-5 0.0 2.5916970944702458 3.3598678899945657E-4 70-71 1.259950458747962E-4 8.399669724986414E-5 0.0 2.7495898861256776 3.3598678899945657E-4 72-73 1.259950458747962E-4 8.399669724986414E-5 0.0 2.948221075947294 3.3598678899945657E-4 74-75 1.259950458747962E-4 8.399669724986414E-5 0.0 3.174802166778802 3.3598678899945657E-4 76-77 1.259950458747962E-4 8.399669724986414E-5 0.0 3.4973704833925927 3.3598678899945657E-4 78-79 1.259950458747962E-4 8.399669724986414E-5 0.0 3.957882376064973 3.3598678899945657E-4 80-81 1.259950458747962E-4 8.399669724986414E-5 0.0 4.464319462958716 3.3598678899945657E-4 82-83 1.259950458747962E-4 8.399669724986414E-5 4.199834862493207E-5 4.945746533246313 3.3598678899945657E-4 84-85 1.259950458747962E-4 8.399669724986414E-5 4.199834862493207E-5 5.3397330416968005 3.569859633119226E-4 86-87 1.259950458747962E-4 8.399669724986414E-5 4.199834862493207E-5 5.689747279136984 3.779851376243886E-4 88-89 1.259950458747962E-4 8.399669724986414E-5 4.199834862493207E-5 6.053452978228896 3.779851376243886E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3470 0.0 58.309143 1 GTATCAA 6480 0.0 41.559464 1 TATCAAC 8445 0.0 31.490442 2 TCAACGC 8465 0.0 31.4134 4 ATCAACG 8495 0.0 31.303778 3 CAACGCA 8625 0.0 30.83066 5 AACGCAG 9045 0.0 29.485336 6 ACGCAGA 10360 0.0 25.106003 7 CGCAGAG 10610 0.0 24.381718 8 TGGTATC 1555 0.0 22.742079 2 GTACATG 8545 0.0 21.636292 1 GCAGAGT 11600 0.0 21.529068 9 TACATGG 8575 0.0 21.335209 2 GAGTACT 6270 0.0 20.629406 12-13 GTGGTAT 1985 0.0 20.196087 1 ACATGGG 8795 0.0 20.157331 3 GTACTTT 6410 0.0 20.068573 14-15 CAGAGTA 10890 0.0 19.94713 10-11 AGAGTAC 10295 0.0 19.726873 10-11 AGTACTT 6450 0.0 18.775232 12-13 >>END_MODULE