FastQCFastQC Report
Fri 27 May 2016
SRR939822_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939822_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2532959
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60360.23829836961435222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46880.18507997958119338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30750.12139951732341502No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37300.053.223191
GTATCAA67300.040.4026681
TATCAAC85850.031.8370742
ATCAACG85900.031.8191723
TCAACGC87000.031.5809544
CAACGCA87700.031.0100775
AACGCAG92100.029.734126
GTACATG86800.026.5566731
TACATGG86250.026.2896772
ACGCAGA105350.025.5924477
CGCAGAG106950.025.166088
ACATGGG91650.024.3305383
GCAGAGT115700.021.5954119
TAAGGTG9550.020.2001725
CATGGGG57650.019.9123344
CAGAGTA105900.019.8160810-11
AGAGTAC99450.019.25610710-11
GAGTACT61700.019.253812-13
GTACTTT62300.019.10971614-15
CATGGGA35500.018.9513134
Produced by FastQC (version 0.11.5)