FastQCFastQC Report
Fri 27 May 2016
SRR939822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2532959
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67300.26569715498750673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59900.2364823117942296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38210.15085123762366465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39450.056.4292561
GTATCAA69700.040.246971
TATCAAC90900.030.5914962
ATCAACG90600.030.4224993
TCAACGC91050.030.272144
CAACGCA92950.029.6533455
AACGCAG97750.028.391356
ACGCAGA111850.024.6002187
CGCAGAG114000.023.9281968
GTACATG88250.023.665541
TACATGG89100.022.6881392
TGGTATC20000.022.5403482
GAGTACT69600.021.3685612-13
GTACTTT70000.021.04313714-15
GCAGAGT125100.020.9328679
ACATGGG93750.020.7472023
GTGGTAT22900.020.7273121
AGAGTAC108900.019.97369410-11
CAGAGTA116750.019.76846310-11
AGTACTT72200.019.4820412-13
Produced by FastQC (version 0.11.5)