##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939822_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2532959 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.360771335027533 31.0 31.0 34.0 30.0 34.0 2 31.58754800215874 31.0 31.0 34.0 30.0 34.0 3 31.627451530008972 31.0 31.0 34.0 30.0 34.0 4 35.445178938940586 37.0 35.0 37.0 33.0 37.0 5 35.16306620043988 37.0 35.0 37.0 32.0 37.0 6 35.189842788612054 37.0 35.0 37.0 32.0 37.0 7 35.089639429615715 37.0 35.0 37.0 32.0 37.0 8 35.05301664969706 37.0 35.0 37.0 32.0 37.0 9 36.63603793034155 39.0 35.0 39.0 32.0 39.0 10-11 36.49219726809633 38.0 35.0 39.0 32.0 39.0 12-13 36.408386791890436 38.0 35.0 39.0 32.0 39.0 14-15 37.44104464383355 39.0 36.0 41.0 32.0 41.0 16-17 37.3489239265223 39.0 36.0 41.0 32.0 41.0 18-19 37.305046390407426 39.0 36.0 40.5 31.5 41.0 20-21 37.210583945496154 39.0 36.0 40.0 31.0 41.0 22-23 37.075460952980286 39.0 36.0 40.0 31.0 41.0 24-25 36.925627102531074 39.0 36.0 40.0 31.0 41.0 26-27 36.62158230749096 38.5 35.0 40.0 30.0 41.0 28-29 36.39409954918339 38.0 35.0 40.0 30.0 41.0 30-31 36.130573767676466 38.0 35.0 40.0 29.5 41.0 32-33 36.13492677931226 38.0 35.0 40.0 30.0 41.0 34-35 35.99853136193677 38.0 35.0 40.0 29.0 41.0 36-37 36.08004018225324 38.0 35.0 40.0 30.0 41.0 38-39 35.997778092736596 38.0 35.0 40.0 29.0 41.0 40-41 35.91625466499852 38.0 35.0 40.0 29.0 41.0 42-43 35.6992114755904 38.0 34.0 40.0 28.0 41.0 44-45 35.46041013691892 38.0 34.0 40.0 27.0 41.0 46-47 35.1914257198794 38.0 34.0 40.0 26.5 41.0 48-49 34.89326218861024 38.0 33.0 40.0 26.0 41.0 50-51 34.65944415207668 38.0 33.0 40.0 26.0 41.0 52-53 34.390307738893526 37.5 33.0 40.0 24.5 41.0 54-55 34.10471724966729 37.0 33.0 40.0 24.0 41.0 56-57 33.71391522721055 37.0 32.0 39.5 23.5 41.0 58-59 33.323937142290895 36.0 31.5 39.0 23.0 41.0 60-61 32.99231629884258 36.0 31.0 39.0 22.0 40.0 62-63 32.67429437270796 35.5 31.0 39.0 22.0 40.0 64-65 32.21095149980714 35.0 31.0 38.5 20.5 40.0 66-67 31.27712469882063 34.5 29.5 38.0 15.5 40.0 68-69 29.385920774872393 34.0 26.0 37.0 8.5 39.0 70-71 28.72581237990824 33.0 25.5 36.0 8.5 39.0 72-73 29.278653937943727 33.0 26.5 36.0 9.0 39.0 74-75 29.37403230766862 33.0 27.5 35.5 9.0 38.0 76-77 28.95870521394148 32.5 27.0 35.0 9.0 37.0 78-79 29.512906643968577 33.5 29.0 35.0 9.0 37.0 80-81 29.345137051172166 34.0 29.0 35.0 7.5 37.0 82-83 28.916738683887104 33.0 28.5 35.0 6.5 36.0 84-85 28.568790296250356 33.0 27.5 35.0 2.0 36.0 86-87 28.183371503447155 33.0 27.0 35.0 2.0 35.5 88-89 27.740093108494847 33.0 26.5 35.0 2.0 35.0 90-91 27.248956062849814 32.0 26.0 34.5 2.0 35.0 92-93 26.74969018448384 32.0 25.0 34.0 2.0 35.0 94-95 25.93321921120713 32.0 24.0 34.0 2.0 35.0 96-97 25.385134540274834 32.0 22.0 34.0 2.0 35.0 98-99 24.693465034372842 31.0 19.0 34.0 2.0 35.0 100-101 23.18794698216592 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.27780728725720394 1101 2 0.2952336075412809 1101 3 0.292172687144177 1101 4 0.24395564748883203 1101 5 0.28425308801784865 1101 6 0.209883512441003 1101 7 0.24787583482002162 1101 8 0.20587375192184254 1101 9 0.33969460036415455 1101 10-11 0.3094775164571857 1101 12-13 0.296352800876015 1101 14-15 0.33466420728048263 1101 16-17 0.32548625360622907 1101 18-19 0.2234729140585472 1101 20-21 0.41011930801374774 1101 22-23 0.3343098194629448 1101 24-25 0.3119769852746188 1101 26-27 0.3408405272678223 1101 28-29 0.2838606309240106 1101 30-31 0.1630901203869186 1101 32-33 0.14479931757741582 1101 34-35 0.2022884438715451 1101 36-37 0.21579508786351909 1101 38-39 0.2364997381401608 1101 40-41 0.28033297538596713 1101 42-43 0.1852360257154544 1101 44-45 0.10203055150788742 1101 46-47 0.005293101859166427 1101 48-49 0.0348679047657825 1101 50-51 -0.029563820994155776 1101 52-53 -0.22964033846317733 1101 54-55 -0.4428288822593842 1101 56-57 -0.3795492974695236 1101 58-59 -0.18918492044073787 1101 60-61 -0.24930674428674848 1101 62-63 -0.242331082422524 1101 64-65 -0.5177951450272822 1101 66-67 -0.5338930813084026 1101 68-69 -8.67639201911701 1101 70-71 -6.8324413830131085 1101 72-73 -1.929466732117703 1101 74-75 -0.6614952562475835 1101 76-77 -0.5207534563681122 1101 78-79 -0.5796927332022328 1101 80-81 -0.6103537218627828 1101 82-83 -0.4196241919884045 1101 84-85 -0.3691875329146974 1101 86-87 -0.5029938817819399 1101 88-89 -0.3572028648581451 1101 90-91 -0.607843649177866 1101 92-93 -0.8294532948158917 1101 94-95 -0.4618503655239756 1101 96-97 -1.0193785374647142 1101 98-99 -0.7198422376497113 1101 100-101 -0.748507647182219 1102 1 0.18700294743605284 1102 2 0.1658934920344386 1102 3 0.2348736220989558 1102 4 0.1826922141772016 1102 5 0.2627568861758718 1102 6 0.24516927711859182 1102 7 0.14296789884152616 1102 8 0.24723147264960943 1102 9 0.2742911530298926 1102 10-11 0.3183760949846217 1102 12-13 0.26569785833508064 1102 14-15 0.3264270019609228 1102 16-17 0.34726947254939233 1102 18-19 0.24775968986859453 1102 20-21 0.27686329214660077 1102 22-23 0.3109374015886246 1102 24-25 0.23901060909818028 1102 26-27 0.08626361660409287 1102 28-29 0.11116601373255719 1102 30-31 0.23323342248519907 1102 32-33 0.0540619941122813 1102 34-35 -0.08464978608052576 1102 36-37 0.01736949642199903 1102 38-39 0.2017003460777005 1102 40-41 0.07045742580748993 1102 42-43 -0.0047596984636015804 1102 44-45 0.015420338795870236 1102 46-47 -0.050845717320704864 1102 48-49 -5.02618263404031E-4 1102 50-51 0.11909523091553353 1102 52-53 0.15216748701494254 1102 54-55 -0.14169350744601417 1102 56-57 -0.23218299187397662 1102 58-59 -0.34947506013315177 1102 60-61 -0.05791674119156909 1102 62-63 -0.027567935644121633 1102 64-65 -0.13055334953087794 1102 66-67 0.10050551907395189 1102 68-69 -4.626044509875449 1102 70-71 -0.16513630020556747 1102 72-73 0.33183326583390027 1102 74-75 0.18306478177834862 1102 76-77 -0.0027483147377971306 1102 78-79 -0.18143614233313698 1102 80-81 -0.13210153746022613 1102 82-83 -0.19111027282835735 1102 84-85 -0.17773962639759588 1102 86-87 -0.41100147342055493 1102 88-89 -0.49461252382634413 1102 90-91 -0.4850842817671044 1102 92-93 -0.4205198935898551 1102 94-95 -0.39271472812907504 1102 96-97 -1.0421589372047784 1102 98-99 -0.6270450684980062 1102 100-101 -0.6882986529259476 1103 1 0.2447753056895685 1103 2 0.18593907221190875 1103 3 0.2034147146082752 1103 4 0.1970748507845883 1103 5 0.20633094760498238 1103 6 0.09723195105498661 1103 7 0.30908149316523037 1103 8 0.23446011995807936 1103 9 0.33957414520082096 1103 10-11 0.28063588291835373 1103 12-13 0.2555422386744013 1103 14-15 0.18536644805126912 1103 16-17 0.24732384443880306 1103 18-19 0.2828201785955713 1103 20-21 0.24708228208541527 1103 22-23 0.13245984628867546 1103 24-25 0.174012035962825 1103 26-27 0.20001119908203435 1103 28-29 0.10060141253526922 1103 30-31 -0.020310885437751836 1103 32-33 0.10840630012744867 1103 34-35 0.012594417629635757 1103 36-37 0.045590017358762225 1103 38-39 0.15039458607554224 1103 40-41 0.17953133103664953 1103 42-43 -0.002916062160672084 1103 44-45 0.09429447502607502 1103 46-47 0.10389214896419219 1103 48-49 0.041706502064606354 1103 50-51 -0.08115127088136376 1103 52-53 -0.011902233009571717 1103 54-55 -0.10856131300769079 1103 56-57 0.11779884441735078 1103 58-59 -0.13611936861253326 1103 60-61 -0.3169917195501313 1103 62-63 -0.29945353528100327 1103 64-65 -0.1348437208574964 1103 66-67 -0.13798286290923656 1103 68-69 -4.643533021429075 1103 70-71 0.07674857606260943 1103 72-73 0.4530636603560154 1103 74-75 0.11148156615803728 1103 76-77 -0.17204369797615016 1103 78-79 -0.19717998396149383 1103 80-81 -0.0025295094125965534 1103 82-83 -0.13889982499953746 1103 84-85 -0.19636621154882405 1103 86-87 -0.36081733236524727 1103 88-89 -0.46900145612618616 1103 90-91 -0.6647790837667813 1103 92-93 -0.5952453115737555 1103 94-95 -0.5212269017884381 1103 96-97 -0.8861968290063089 1103 98-99 -0.5007732710539621 1103 100-101 -0.5619185091584065 1104 1 0.18294705041501658 1104 2 0.226850194624717 1104 3 0.2382121316563719 1104 4 0.23547776662288555 1104 5 0.22385911195222974 1104 6 0.18113473589632179 1104 7 0.19560575842637462 1104 8 0.2824065499055308 1104 9 0.2873518202358838 1104 10-11 0.27538290466385007 1104 12-13 0.1818448878953589 1104 14-15 0.27865246836350366 1104 16-17 0.27201484571795476 1104 18-19 0.20661885937908409 1104 20-21 0.14471932802681664 1104 22-23 0.27341489121292284 1104 24-25 0.18980680224883173 1104 26-27 0.3042851676735836 1104 28-29 0.10229204394063629 1104 30-31 0.007404219282754809 1104 32-33 0.05873803067439809 1104 34-35 0.14404524753393133 1104 36-37 0.1146146293765753 1104 38-39 0.14157617869039285 1104 40-41 0.060181923605270526 1104 42-43 0.10680738013131474 1104 44-45 0.15524890349401943 1104 46-47 0.15916516204847397 1104 48-49 0.18923489758297052 1104 50-51 0.15039433917920064 1104 52-53 0.07170812037399799 1104 54-55 0.0057281440484260315 1104 56-57 0.19287628674015878 1104 58-59 0.050527032053231835 1104 60-61 -0.0485261563402446 1104 62-63 0.10927895350719297 1104 64-65 0.23639204285480275 1104 66-67 0.34841348766945757 1104 68-69 -4.3608646903303985 1104 70-71 0.652344226963411 1104 72-73 0.8489888842067117 1104 74-75 0.6605838516697737 1104 76-77 0.5220868674683885 1104 78-79 0.3245974907252709 1104 80-81 0.15951026743022467 1104 82-83 0.24357778993103452 1104 84-85 0.29913404273940003 1104 86-87 0.27684005663409295 1104 88-89 0.19269464632884947 1104 90-91 0.3718187709652909 1104 92-93 0.16074202024926265 1104 94-95 0.6028295347859114 1104 96-97 0.002673052479970295 1104 98-99 0.42441705982895783 1104 100-101 0.49702357477211834 1105 1 0.13004795165658578 1105 2 0.23635419664148216 1105 3 0.24223575661023133 1105 4 0.0596937944974556 1105 5 0.19824935585467074 1105 6 0.24652111733488624 1105 7 0.09926474518235295 1105 8 0.2584079544378426 1105 9 0.18522836544651966 1105 10-11 0.1678267908967328 1105 12-13 0.21483249019662765 1105 14-15 0.2311635200520925 1105 16-17 0.33632464413154395 1105 18-19 0.19788757522238853 1105 20-21 0.36536122630626267 1105 22-23 0.2370082430932854 1105 24-25 0.23418079499207778 1105 26-27 0.3541183794608571 1105 28-29 0.35297405242184965 1105 30-31 0.37096245785517823 1105 32-33 0.31653160944075154 1105 34-35 0.2745303513883002 1105 36-37 0.17341501472262166 1105 38-39 0.34406562218948267 1105 40-41 0.27601329869643365 1105 42-43 0.21832567056338803 1105 44-45 0.3445839114620455 1105 46-47 0.36430250910301254 1105 48-49 0.3894588574637652 1105 50-51 0.35230036156168865 1105 52-53 0.3028754577948831 1105 54-55 0.2696181583638477 1105 56-57 0.16026637758459117 1105 58-59 0.32049187372822985 1105 60-61 0.21344455555956898 1105 62-63 0.285275181077445 1105 64-65 0.2807678362805106 1105 66-67 0.9742829922116805 1105 68-69 2.177875192085036 1105 70-71 2.5363989587067373 1105 72-73 1.4576654726337246 1105 74-75 0.8221996040399233 1105 76-77 0.5565739880871057 1105 78-79 0.5859204071105815 1105 80-81 0.6010337798414263 1105 82-83 0.45728751091528963 1105 84-85 0.34943363128345695 1105 86-87 0.3612864368121791 1105 88-89 0.5680988995408178 1105 90-91 0.48102768614414515 1105 92-93 0.39504277023350554 1105 94-95 0.7669261774134668 1105 96-97 0.3508521123436559 1105 98-99 0.6776679669227477 1105 100-101 0.7203727991281497 1106 1 0.4202675074417854 1106 2 0.3301568569121045 1106 3 0.3190041127038121 1106 4 0.16336451959791276 1106 5 0.3819145585325856 1106 6 0.36059286052947925 1106 7 0.16766198354581974 1106 8 0.19461258297518924 1106 9 0.37828047138651044 1106 10-11 0.37481283766691575 1106 12-13 0.39841134806669487 1106 14-15 0.5683193387884486 1106 16-17 0.503677133102272 1106 18-19 0.37513463239844924 1106 20-21 0.3870419746947604 1106 22-23 0.4802528060663036 1106 24-25 0.5730736100257019 1106 26-27 0.582721839905588 1106 28-29 0.6163680913950316 1106 30-31 0.5330745688042242 1106 32-33 0.4763128662232319 1106 34-35 0.6678671749655791 1106 36-37 0.7456041343047914 1106 38-39 0.7831431710661789 1106 40-41 0.5092121710651369 1106 42-43 0.5345432151296876 1106 44-45 0.6784446543736848 1106 46-47 0.572142435397808 1106 48-49 0.6846536056338621 1106 50-51 0.5607878359449217 1106 52-53 0.668378421236774 1106 54-55 0.6998611221733029 1106 56-57 0.8098729201668533 1106 58-59 0.9599824044290912 1106 60-61 0.9392101044216972 1106 62-63 0.754558680166987 1106 64-65 0.7109830057190933 1106 66-67 1.209306800444498 1106 68-69 2.754799151090925 1106 70-71 3.154382341925654 1106 72-73 2.0673018873299895 1106 74-75 1.5609293283881094 1106 76-77 1.0278424305254958 1106 78-79 0.9332139568268865 1106 80-81 0.8028851583283121 1106 82-83 0.9797647275049712 1106 84-85 0.9566940069752619 1106 86-87 0.8957919331869881 1106 88-89 0.9903610818667268 1106 90-91 0.666942941866786 1106 92-93 0.7852792304639351 1106 94-95 1.2881688227267674 1106 96-97 1.1593695530076111 1106 98-99 1.0137253004826867 1106 100-101 1.1867394840358223 1107 1 0.027927313885790284 1107 2 -0.03000468243810417 1107 3 0.06477203083362326 1107 4 -0.004987468550936569 1107 5 -0.02297115389644233 1107 6 0.02073144488952039 1107 7 -0.06241666512429589 1107 8 -0.03286369787114296 1107 9 -9.887656774694165E-4 1107 10-11 0.053899217024145685 1107 12-13 -0.08837554297923589 1107 14-15 0.018991080623933954 1107 16-17 -0.021167431829958616 1107 18-19 -0.022481523462026587 1107 20-21 -0.042051549892050843 1107 22-23 -0.0019652747641458745 1107 24-25 -0.025133809821362263 1107 26-27 0.0721324592510797 1107 28-29 -0.050273704858526 1107 30-31 0.0465128653092961 1107 32-33 0.009359553002838084 1107 34-35 0.12055402196680376 1107 36-37 0.042232097393885226 1107 38-39 0.14104531361216033 1107 40-41 0.021044445600800543 1107 42-43 -0.04497574394724779 1107 44-45 0.030947410175912182 1107 46-47 0.009989994406872427 1107 48-49 -0.05271325241464808 1107 50-51 0.024007729106443776 1107 52-53 0.03776723484586597 1107 54-55 -0.15971390664829954 1107 56-57 0.0026949127923785454 1107 58-59 0.18050366923399963 1107 60-61 0.18808807773975644 1107 62-63 0.24811473607246626 1107 64-65 -0.019112189798448753 1107 66-67 0.5681075120191075 1107 68-69 1.7402119016703637 1107 70-71 2.2433302211613473 1107 72-73 1.3224584114811648 1107 74-75 0.7487993008541558 1107 76-77 0.40120835412839995 1107 78-79 0.3842752264935676 1107 80-81 0.31157398312908313 1107 82-83 0.38006064530745576 1107 84-85 0.2366171002369022 1107 86-87 0.1904392386381808 1107 88-89 0.20058539393906472 1107 90-91 0.1943424904687845 1107 92-93 0.04450575259339118 1107 94-95 0.4654478350665059 1107 96-97 0.1062904253814736 1107 98-99 0.33304673833753995 1107 100-101 0.6247088127994154 1108 1 -0.10522374180392902 1108 2 -0.12105880255558432 1108 3 -0.11870652499130685 1108 4 -0.10519554371312978 1108 5 0.037919416027868635 1108 6 0.003020218765698246 1108 7 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-0.6484507590391217 2316 56-57 -0.5829661522541159 2316 58-59 -0.7325353362123295 2316 60-61 -0.7420276247107509 2316 62-63 -0.7085568976626533 2316 64-65 -0.4894969165864218 2316 66-67 -0.02993400022640813 2316 68-69 1.4498054686769315 2316 70-71 1.6137602564542881 2316 72-73 0.5750112316593317 2316 74-75 0.005527453895702195 2316 76-77 -0.2727464967315889 2316 78-79 -0.42798318146211756 2316 80-81 -0.5069101274864813 2316 82-83 -0.5944078085732549 2316 84-85 -0.6517370581416237 2316 86-87 -0.5629873988076994 2316 88-89 -0.5656616165138786 2316 90-91 -0.44553203289039445 2316 92-93 -0.38067705181113354 2316 94-95 -0.23472415113272405 2316 96-97 -0.3822022903602935 2316 98-99 -0.6293202297266518 2316 100-101 -0.4738486725843707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 24.0 4 48.0 5 93.0 6 228.0 7 658.0 8 1469.0 9 2468.0 10 3907.0 11 5712.0 12 7906.0 13 10145.0 14 12782.0 15 15683.0 16 19626.0 17 22815.0 18 22084.0 19 19747.0 20 20164.0 21 20852.0 22 22945.0 23 25962.0 24 31604.0 25 38807.0 26 48171.0 27 59300.0 28 73333.0 29 88297.0 30 105027.0 31 124718.0 32 147923.0 33 179147.0 34 221776.0 35 279142.0 36 344123.0 37 360510.0 38 183544.0 39 12214.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.626865318017224 18.908904956148493 14.385427320220659 24.078802405613626 2 16.52178926389538 20.283125971668447 38.214305881721124 24.98077888271505 3 19.812930105011652 23.60351952283438 29.457231212373525 27.126319159780447 4 14.036034535103015 16.65194738643618 35.312770558070625 33.999247520390185 5 14.473270195056454 36.75353608171313 33.63264071783239 15.140553005398036 6 33.49009340068015 35.35281674627056 16.585983660210708 14.57110619283857 7 29.51966455043291 31.78764441114128 19.928549968633526 18.764141069792288 8 27.0418115729469 34.96061325903814 18.832203758529058 19.1653714094859 9 26.399203461248288 16.241636757641952 19.139709722897212 38.21945005821255 10-11 25.731427156933844 25.858531464583518 26.67869870771694 21.731342670765695 12-13 26.37269296502628 22.976980677539586 27.15584816019525 23.494478197238884 14-15 23.13624105246078 24.52082722223297 24.981178139875144 27.36175358543111 16-17 23.130691366208193 27.78911612296894 25.687336319708038 23.392856191114824 18-19 23.13535276330963 26.406507172046606 27.698849448411917 22.759290616231844 20-21 23.739270947536063 25.56740950011429 27.862176213669464 22.831143338680178 22-23 23.512303199538565 25.386770966288836 27.767366151603717 23.333559682568886 24-25 23.312181523664616 25.672701374163577 27.83418523552888 23.180931866642926 26-27 23.08828104396602 25.96189985139892 27.72730754107059 23.222511563564467 28-29 23.04746526540615 26.048068315398105 27.72871238463338 23.175754034562367 30-31 23.18117215104917 25.90603841370734 27.67182534199254 23.240964093250952 32-33 22.989217527528645 25.933028729440128 27.678690927065226 23.399062815966 34-35 23.31207346941515 25.99713723222748 27.609600754245143 23.081188544112226 36-37 23.420671689007428 25.77936451041662 27.602357230617596 23.197606569958353 38-39 23.090556070030907 25.942420164500835 27.675489715882495 23.291534049585763 40-41 23.326753332977656 25.786764517002286 27.73815945731793 23.148322692702127 42-43 23.138119455251456 25.79865028097575 27.651218977714205 23.412011286058586 44-45 23.045979532423726 25.948898633848362 27.637787838875433 23.36733399485248 46-47 23.225088168176473 26.105367145129264 27.319430586701177 23.350114099993085 48-49 23.178175307474564 26.26166372243724 27.193141384458936 23.36701958562926 50-51 23.126458228741942 26.385679114499393 27.166358152932325 23.32150450382634 52-53 23.283697723541486 26.260174983945024 27.058291568662657 23.397835723850832 54-55 23.152780290634606 26.361999126184216 27.02996255865271 23.455258024528465 56-57 23.012401602206836 26.378121997960218 27.09978644482467 23.509689955008277 58-59 23.02226079457825 26.13596071094924 27.073095228882092 23.76868326559041 60-61 22.895918686838076 26.26339638330471 27.22814721605353 23.61253771380368 62-63 22.868735959824864 26.283879016625143 27.24123636449775 23.606148659052238 64-65 22.737895927311506 26.22839106606015 27.192251529955264 23.841461476673082 66-67 22.552482908989692 26.250321003186606 27.307283920621717 23.889912167201985 68-69 22.421113137239356 26.06231665741695 27.764270990248157 23.752299215095537 70-71 22.379662289272638 25.989976919051006 27.549343034188617 24.081017757487732 72-73 22.453526143947883 26.13837143609144 27.193368061549744 24.214734358410933 74-75 22.57647888130446 26.376461900258075 26.798401412191208 24.24865780624626 76-77 22.536512006710513 26.200972179137345 26.9063026171669 24.356213196985248 78-79 22.562255029211425 26.269969036580658 26.89792336825149 24.269852565956427 80-81 22.52556479629393 26.29010356202598 26.780721825129945 24.403609816550144 82-83 22.409885991003403 26.30402872846688 26.720577285529405 24.565507995000313 84-85 22.447990989164612 26.18254877069252 26.679084332678432 24.69037590746444 86-87 22.36282996826209 26.171458147530945 26.835490713729893 24.63022117047707 88-89 22.316929311050014 26.14022904464191 26.677644617593042 24.865197026715034 90-91 22.22136902193374 26.150132632068495 26.709106033406936 24.919392312590826 92-93 22.129866202322525 25.902116217769294 26.80256931653014 25.165448263378043 94-95 21.880539500559173 25.76213353731306 27.05914588889914 25.298181073228633 96-97 22.003514279227254 25.610092694050408 27.05996979299316 25.326423233729184 98-99 21.953105734704398 25.700019091923366 26.832720990000137 25.5141541833721 100-101 21.797870017613636 25.640177249785506 26.96033122003935 25.60162151256151 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 356.0 1 246.5 2 183.0 3 245.5 4 232.0 5 231.5 6 456.0 7 1008.5 8 1660.0 9 2069.0 10 1919.5 11 1652.0 12 1710.5 13 1812.0 14 1923.0 15 2101.5 16 2297.0 17 2562.5 18 2814.0 19 3072.0 20 3461.5 21 4048.5 22 4830.5 23 5751.0 24 6942.0 25 8606.5 26 10819.0 27 13634.0 28 16238.5 29 18675.0 30 21709.5 31 25451.5 32 29694.0 33 32680.5 34 35271.0 35 39396.0 36 44261.0 37 48626.0 38 53175.5 39 57581.0 40 62223.0 41 68174.5 42 73520.5 43 78225.0 44 83414.5 45 88207.5 46 92667.5 47 97919.0 48 102066.0 49 103944.5 50 104209.0 51 103816.5 52 104458.0 53 104416.0 54 102776.0 55 98161.5 56 90142.0 57 81553.5 58 74105.5 59 66400.0 60 56558.5 61 47009.5 62 37667.5 63 28080.0 64 20760.0 65 15321.0 66 10916.0 67 8110.5 68 5851.0 69 4006.5 70 2878.5 71 1963.5 72 1325.0 73 961.0 74 691.0 75 470.5 76 302.5 77 186.5 78 108.5 79 63.5 80 37.0 81 21.0 82 13.0 83 7.0 84 6.5 85 6.5 86 5.5 87 2.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021674255287985316 2 0.02321395648330668 3 7.895903565750572E-5 4 0.0 5 0.0 6 3.947951782875286E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.14534937201905E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.1843855348625857E-4 28-29 5.9219276743129284E-5 30-31 3.553156604587757E-4 32-33 0.0020529349270951487 34-35 0.0037702939526458976 36-37 0.09996213914240223 38-39 0.09212545485339478 40-41 0.10122548371292231 42-43 0.10187689575709674 44-45 0.0955404331455819 46-47 0.09017121872087151 48-49 0.09090158980070345 50-51 0.08991460185498462 52-53 0.07155662606461455 54-55 0.060522100831478125 56-57 0.032629821485464235 58-59 0.013620433650919734 60-61 0.009178987895185039 62-63 0.0019344963736088899 64-65 0.004875720451850978 66-67 0.14917335811594268 68-69 0.4089683251880508 70-71 0.39015633494265006 72-73 0.17637474589995336 74-75 0.028583170908017066 76-77 0.03207710823586169 78-79 0.005191556594481001 80-81 0.0012830843294344679 82-83 3.947951782875286E-5 84-85 3.3557590154439926E-4 86-87 0.0010264674635475743 88-89 1.1843855348625857E-4 90-91 4.737542139450343E-4 92-93 0.002664867453440818 94-95 0.07850502120247506 96-97 0.01713411073767874 98-99 0.01873303120974323 100-101 0.010343633671133248 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2532959.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.45438819631135 #Duplication Level Percentage of deduplicated Percentage of total 1 72.9528416731289 38.26696677146343 2 14.352247030380447 15.056766744418654 3 5.190790475803969 8.16839215990611 4 2.4447386167489116 5.129490737658426 5 1.3522696239148932 3.5466237899455884 6 0.8053019029275715 2.5344971178834648 7 0.5551441948784516 2.038382437215811 8 0.4065908438536618 1.7061979168452632 9 0.2913489672064856 1.3754278643799045 >10 1.5180394662288628 14.240289899135838 >50 0.09559627839945094 3.4041589213899637 >100 0.03327931067014118 3.2329523072849207 >500 0.0014341958878680034 0.5388042324872103 >1k 2.2645198229389135E-4 0.2574049320537636 >5k 1.5096798819592757E-4 0.5036441679316832 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6730 0.26569715498750673 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5990 0.2364823117942296 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3821 0.15085123762366465 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.947951782875285E-5 0.0 2 0.0 0.0 0.0 3.947951782875285E-5 0.0 3 0.0 0.0 0.0 3.947951782875285E-5 0.0 4 0.0 0.0 0.0 3.947951782875285E-5 0.0 5 0.0 0.0 0.0 3.947951782875285E-5 0.0 6 0.0 0.0 0.0 3.947951782875285E-5 0.0 7 0.0 0.0 0.0 7.89590356575057E-5 0.0 8 0.0 0.0 0.0 7.89590356575057E-5 0.0 9 0.0 0.0 0.0 7.89590356575057E-5 0.0 10-11 0.0 0.0 0.0 1.38178312400635E-4 0.0 12-13 0.0 0.0 0.0 1.9739758914376427E-4 3.947951782875285E-5 14-15 0.0 0.0 0.0 1.9739758914376427E-4 3.947951782875285E-5 16-17 0.0 0.0 0.0 2.7635662480127E-4 3.947951782875285E-5 18-19 0.0 0.0 0.0 2.7635662480127E-4 3.947951782875285E-5 20-21 0.0 0.0 0.0 4.342746961162814E-4 3.947951782875285E-5 22-23 0.0 0.0 0.0 7.106313209175514E-4 3.947951782875285E-5 24-25 0.0 0.0 0.0 0.00110542649920508 3.947951782875285E-5 26-27 0.0 0.0 0.0 0.0021121542038382776 3.947951782875285E-5 28-29 0.0 0.0 0.0 0.006691778271973609 3.947951782875285E-5 30-31 0.0 0.0 0.0 0.02214800950193035 3.947951782875285E-5 32-33 0.0 0.0 0.0 0.05552794182614089 3.947951782875285E-5 34-35 0.0 0.0 0.0 0.10596302585237266 3.947951782875285E-5 36-37 0.0 0.0 0.0 0.19350885663763212 7.89590356575057E-5 38-39 0.0 0.0 0.0 0.3316476895204383 7.89590356575057E-5 40-41 0.0 0.0 0.0 0.4812355825735829 7.89590356575057E-5 42-43 0.0 0.0 3.947951782875285E-5 0.6269150033616809 7.89590356575057E-5 44-45 0.0 0.0 3.947951782875285E-5 0.8041780384127812 7.89590356575057E-5 46-47 0.0 0.0 3.947951782875285E-5 1.0188084370887962 9.869879457188214E-5 48-49 0.0 0.0 3.947951782875285E-5 1.248065997120364 1.1843855348625857E-4 50-51 0.0 0.0 3.947951782875285E-5 1.4888910558757562 1.1843855348625857E-4 52-53 0.0 0.0 3.947951782875285E-5 1.7248996134560408 1.1843855348625857E-4 54-55 0.0 0.0 3.947951782875285E-5 1.970442474591969 1.1843855348625857E-4 56-57 0.0 0.0 3.947951782875285E-5 2.2476084295087286 1.1843855348625857E-4 58-59 0.0 0.0 3.947951782875285E-5 2.5448299794824947 1.1843855348625857E-4 60-61 0.0 0.0 3.947951782875285E-5 2.849592117361552 1.1843855348625857E-4 62-63 0.0 0.0 3.947951782875285E-5 3.142668317963299 1.1843855348625857E-4 64-65 0.0 0.0 3.947951782875285E-5 3.442179679971132 1.1843855348625857E-4 66-67 0.0 0.0 3.947951782875285E-5 3.7603845936708806 1.1843855348625857E-4 68-69 0.0 0.0 3.947951782875285E-5 4.122944745651232 1.1843855348625857E-4 70-71 0.0 0.0 3.947951782875285E-5 4.528162516645551 1.1843855348625857E-4 72-73 0.0 0.0 3.947951782875285E-5 4.941710465901738 1.38178312400635E-4 74-75 0.0 0.0 3.947951782875285E-5 5.358614174173368 1.579180713150114E-4 76-77 0.0 0.0 3.947951782875285E-5 5.800804513614314 1.579180713150114E-4 78-79 0.0 0.0 3.947951782875285E-5 6.299233426202319 1.579180713150114E-4 80-81 0.0 0.0 3.947951782875285E-5 6.823817519351873 1.579180713150114E-4 82-83 0.0 0.0 3.947951782875285E-5 7.357837217262498 1.579180713150114E-4 84-85 0.0 0.0 3.947951782875285E-5 7.868109985199128 1.579180713150114E-4 86-87 0.0 0.0 3.947951782875285E-5 8.381639813356632 1.7765783022938785E-4 88-89 0.0 0.0 3.947951782875285E-5 8.953816465248746 1.9739758914376427E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3945 0.0 56.429256 1 GTATCAA 6970 0.0 40.24697 1 TATCAAC 9090 0.0 30.591496 2 ATCAACG 9060 0.0 30.422499 3 TCAACGC 9105 0.0 30.27214 4 CAACGCA 9295 0.0 29.653345 5 AACGCAG 9775 0.0 28.39135 6 ACGCAGA 11185 0.0 24.600218 7 CGCAGAG 11400 0.0 23.928196 8 GTACATG 8825 0.0 23.66554 1 TACATGG 8910 0.0 22.688139 2 TGGTATC 2000 0.0 22.540348 2 GAGTACT 6960 0.0 21.36856 12-13 GTACTTT 7000 0.0 21.043137 14-15 GCAGAGT 12510 0.0 20.932867 9 ACATGGG 9375 0.0 20.747202 3 GTGGTAT 2290 0.0 20.727312 1 AGAGTAC 10890 0.0 19.973694 10-11 CAGAGTA 11675 0.0 19.768463 10-11 AGTACTT 7220 0.0 19.48204 12-13 >>END_MODULE