FastQCFastQC Report
Fri 27 May 2016
SRR939821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255691
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74570.5938562910779802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58950.4694626305356971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39690.31608094666601894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27610.21987893518389479No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18130.14438265464990988No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15010.11953577751214271No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13450.10711233894325912No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG72750.027.0626931
TACATGG75450.026.1571242
ACATGGG77300.024.6718923
GTATCAA106450.024.4225391
CATGGGA48100.023.6712994
GAGTACT55500.022.054212-13
GGTATCA80500.021.6874161
TATCAAC125300.021.0513342
GTACTTT59000.020.82838814-15
TCAACGC128400.020.7647724
ATCAACG128350.020.6989363
AGTACTT58250.020.64650712-13
ATACCGT1150.00375026820.626626
CAACGCA130050.020.5378025
ATGGGAT17850.020.4648445
AACGCAG131350.020.3345346
CATGGGG30800.019.7158364
GTATAGG4350.019.6308541
ACTTTTT69550.019.00150116-17
ATAGGAC3003.774403E-918.9764923
Produced by FastQC (version 0.11.5)