FastQCFastQC Report
Fri 27 May 2016
SRR939821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255691
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81170.6464169927155646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74310.5917857179831663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54050.4304402914411269No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32090.2555565023560733No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21180.16867206980061178No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17840.1420731692749251No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15910.12670314591726786No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15520.12359728627504697No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15140.12057106405954968No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCG856.682559E-427.7410325
GGTATCA78100.026.8804341
GTATCAA105050.024.6549191
TACATGG79450.024.0456492
GTACATG79750.023.8397481
ACATGGG78550.023.1773933
GAGTACT61150.022.5812812-13
GGACCGT2103.7480277E-822.440936
ATGGGAG17500.022.0977125
GTACTTT63650.021.65689714-15
CATGGGA49500.021.5315254
AGTACTT63250.020.67663412-13
ATCAACG125900.020.566963
TCAACGC126550.020.4216084
TATCAAC127900.020.2108882
CAACGCA128450.020.0828235
AACGCAG128750.020.0216666
ACTTTTT71150.019.83727616-17
TACACCG1456.451301E-419.514385
TACCTGG8950.019.5008742
Produced by FastQC (version 0.11.5)