##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939821_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1255691 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.391006226850397 31.0 31.0 34.0 30.0 34.0 2 31.59179288535157 31.0 31.0 34.0 30.0 34.0 3 31.637523084899073 31.0 31.0 34.0 30.0 34.0 4 35.455647926121955 37.0 35.0 37.0 33.0 37.0 5 35.18795069806186 37.0 35.0 37.0 32.0 37.0 6 35.22723345154182 37.0 35.0 37.0 32.0 37.0 7 35.138590624604305 37.0 35.0 37.0 32.0 37.0 8 35.08818331898532 37.0 35.0 37.0 32.0 37.0 9 36.66939000120252 39.0 35.0 39.0 32.0 39.0 10-11 36.52268273006655 38.0 35.0 39.0 32.0 39.0 12-13 36.44811223461823 38.0 35.0 39.0 32.0 39.0 14-15 37.43290387523682 39.0 36.0 41.0 32.0 41.0 16-17 37.36710464596784 39.0 36.0 41.0 32.0 41.0 18-19 37.36873960233847 39.0 36.0 41.0 32.0 41.0 20-21 37.31415212819077 39.0 36.0 40.5 31.5 41.0 22-23 37.1575132735681 39.0 36.0 40.0 31.0 41.0 24-25 36.97600683607671 39.0 36.0 40.0 31.0 41.0 26-27 36.647724639262364 38.5 35.0 40.0 30.0 41.0 28-29 36.40009803367229 38.0 35.0 40.0 30.0 41.0 30-31 36.12003550236483 38.0 35.0 40.0 30.0 41.0 32-33 36.037266732022445 38.0 35.0 40.0 30.0 41.0 34-35 36.06784551294865 38.0 35.0 40.0 30.0 41.0 36-37 36.0102939337783 38.0 35.0 40.0 30.0 41.0 38-39 35.84510918689391 38.0 35.0 40.0 29.0 41.0 40-41 35.64207356746206 38.0 34.0 40.0 28.5 41.0 42-43 35.396664466019104 38.0 34.0 40.0 27.0 41.0 44-45 35.091177288043 38.0 34.0 40.0 26.5 41.0 46-47 34.707230520884515 38.0 33.0 40.0 25.5 41.0 48-49 34.338896273048064 38.0 33.0 40.0 24.0 41.0 50-51 34.03134927302975 37.5 33.0 40.0 23.5 41.0 52-53 33.708406765677225 37.0 33.0 40.0 23.0 41.0 54-55 33.36508981907173 37.0 32.0 40.0 22.0 41.0 56-57 32.088213581207484 35.5 30.5 39.5 14.0 40.5 58-59 28.784141958491382 34.0 23.0 38.0 6.5 40.0 60-61 28.77880545452663 33.5 24.0 38.0 6.5 40.0 62-63 30.478518202328438 34.5 28.5 38.0 3.5 40.0 64-65 29.930189831734083 34.0 28.0 38.0 2.0 40.0 66-67 24.513292681081573 28.0 16.5 35.5 2.0 38.0 68-69 16.95478943466187 13.0 7.0 29.0 2.0 34.5 70-71 15.852727701321424 12.5 7.0 25.5 2.0 33.0 72-73 17.902140335480624 16.5 7.0 29.0 2.0 34.0 74-75 18.640012152671318 17.5 7.5 30.5 2.0 34.5 76-77 19.680204365564457 22.5 7.5 30.5 2.0 34.5 78-79 22.775031835061334 27.5 10.5 33.5 2.0 35.5 80-81 25.341366227837902 31.5 20.0 34.5 2.0 36.0 82-83 26.234431082169102 32.5 24.0 35.0 2.0 36.0 84-85 26.214364441570417 33.0 24.0 35.0 2.0 35.5 86-87 25.92840117512987 33.0 23.5 35.0 2.0 35.0 88-89 25.495656176559358 32.5 21.0 35.0 2.0 35.0 90-91 23.96888725012762 31.5 9.5 34.0 2.0 35.0 92-93 21.952698554023243 30.5 2.0 34.0 2.0 35.0 94-95 17.5565891608684 20.0 2.0 33.0 2.0 35.0 96-97 16.232927925739695 12.5 2.0 32.5 2.0 34.5 98-99 15.12723273480498 2.0 2.0 31.5 2.0 34.0 100-101 14.532357084664937 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5852349515658908 1101 2 0.5766888943911326 1101 3 0.5915053608978376 1101 4 0.46457855374129764 1101 5 0.5679387337518662 1101 6 0.6458114794890761 1101 7 0.574210150660349 1101 8 0.6023406418206392 1101 9 0.7235600918062559 1101 10-11 0.8300041431056755 1101 12-13 0.8777260112806786 1101 14-15 0.8410244108095952 1101 16-17 0.6855020084453827 1101 18-19 0.7935740110563998 1101 20-21 0.8557539524805833 1101 22-23 0.8594081979987749 1101 24-25 1.069426068412838 1101 26-27 0.9536512196956792 1101 28-29 0.8476070534767359 1101 30-31 0.932441155075459 1101 32-33 0.9318904303442892 1101 34-35 1.0499875085603634 1101 36-37 1.082479173459241 1101 38-39 1.0839029973276268 1101 40-41 0.8324593573491939 1101 42-43 1.1134744791413311 1101 44-45 0.9460811138937046 1101 46-47 0.6862888788260548 1101 48-49 0.7038446710002546 1101 50-51 0.7442721434423518 1101 52-53 0.663006456489704 1101 54-55 0.8715495346504198 1101 56-57 1.187605691116648 1101 58-59 -8.229774248538472 1101 60-61 -9.510050648387061 1101 62-63 -2.227843714519903 1101 64-65 0.48408126740323 1101 66-67 -1.0485273474504098 1101 68-69 -3.575853244899559 1101 70-71 -4.181924251715044 1101 72-73 -6.485823391843919 1101 74-75 -7.241988845003153 1101 76-77 -8.000512047675098 1101 78-79 -11.299535213978892 1101 80-81 -7.311987377219449 1101 82-83 -3.4395649220683033 1101 84-85 -2.428424349405578 1101 86-87 -2.260120052610496 1101 88-89 -2.3331175585170456 1101 90-91 -8.12021811418425 1101 92-93 -13.340106071131489 1101 94-95 -11.447579780979094 1101 96-97 -11.956004771246977 1101 98-99 -11.495228276364406 1101 100-101 -11.256332753176958 1102 1 0.6370992872628101 1102 2 0.7848801664575547 1102 3 0.5773859595356008 1102 4 0.5558226609493389 1102 5 0.5210371579396877 1102 6 0.6809235881572135 1102 7 0.5652981522757727 1102 8 0.739865009049339 1102 9 0.9202026565978443 1102 10-11 0.936697655792166 1102 12-13 0.7717800191558553 1102 14-15 1.0663251664849298 1102 16-17 1.1510248068517086 1102 18-19 1.0767328582049416 1102 20-21 0.8971355258074993 1102 22-23 1.1055269159136358 1102 24-25 1.1237504263217915 1102 26-27 1.1841690864079126 1102 28-29 1.117411494331364 1102 30-31 1.103871890014382 1102 32-33 1.0926520081757403 1102 34-35 1.1345000474896736 1102 36-37 1.0325855737885306 1102 38-39 0.958766113692306 1102 40-41 0.9636773692939897 1102 42-43 1.1571840450745796 1102 44-45 0.9738112143138764 1102 46-47 0.9111100563748096 1102 48-49 1.1357368105788481 1102 50-51 1.0510739980699881 1102 52-53 1.04405554820368 1102 54-55 0.9404282149756753 1102 56-57 1.8701579787237286 1102 58-59 -9.028048543927532 1102 60-61 -9.560392760394766 1102 62-63 -2.5189243380102972 1102 64-65 0.2504374056149281 1102 66-67 -0.7180591860015042 1102 68-69 -3.2961291091109928 1102 70-71 -4.132269470301706 1102 72-73 -6.235883581811223 1102 74-75 -7.480298400063383 1102 76-77 -8.462281710378289 1102 78-79 -11.502324921882806 1102 80-81 -7.241994211693157 1102 82-83 -2.8045335844530683 1102 84-85 -1.8522316172470497 1102 86-87 -1.6751046362124207 1102 88-89 -1.865194927865982 1102 90-91 -7.914562587191963 1102 92-93 -13.90511706065455 1102 94-95 -11.968741408515811 1102 96-97 -11.77943653135695 1102 98-99 -11.377238465943385 1102 100-101 -11.115653266694459 1103 1 0.22848681337287147 1103 2 0.3052974455252091 1103 3 0.3927630471819974 1103 4 0.2139833636180768 1103 5 0.20308010910391516 1103 6 0.20834401618611764 1103 7 0.39714844038996944 1103 8 0.3532146401260121 1103 9 0.30101104443718185 1103 10-11 0.2751535341929596 1103 12-13 0.2797358261999676 1103 14-15 0.3113019338602854 1103 16-17 0.3026466423680674 1103 18-19 0.45470226935474756 1103 20-21 0.30733499091036975 1103 22-23 0.34751365283729285 1103 24-25 0.25617963029627333 1103 26-27 0.059468182124099656 1103 28-29 0.20146364587442633 1103 30-31 0.41546707334546085 1103 32-33 0.4228444117469152 1103 34-35 0.41755580022856975 1103 36-37 0.16414274346731617 1103 38-39 0.24722088004086373 1103 40-41 0.4226162305154304 1103 42-43 0.5668182169570812 1103 44-45 0.4978746346529377 1103 46-47 0.34783752758655595 1103 48-49 0.13035911130371858 1103 50-51 0.4186225530507741 1103 52-53 0.6286526053427721 1103 54-55 0.4885629326904848 1103 56-57 1.0079268868552411 1103 58-59 -6.010674581147921 1103 60-61 -8.553517497440751 1103 62-63 -2.3561815167708424 1103 64-65 0.04142209080221093 1103 66-67 -1.5416589732288415 1103 68-69 -4.278875155301934 1103 70-71 -4.626447413486957 1103 72-73 -7.502887323866998 1103 74-75 -8.113315601658064 1103 76-77 -8.835302952429434 1103 78-79 -12.103475339921776 1103 80-81 -8.588507371904672 1103 82-83 -4.912823841207398 1103 84-85 -3.9560111121158528 1103 86-87 -3.6237556241781625 1103 88-89 -3.6064421500521675 1103 90-91 -8.760268582821597 1103 92-93 -14.07730213209464 1103 94-95 -12.149290966197032 1103 96-97 -12.144766078770225 1103 98-99 -11.74191143918185 1103 100-101 -11.414278677458753 1104 1 0.06504057638662175 1104 2 0.0017956185915153355 1104 3 0.09207169530102988 1104 4 0.20625992835319096 1104 5 -0.001553952055019181 1104 6 -0.01415966990805373 1104 7 0.05577340110577467 1104 8 0.0485451544904123 1104 9 0.040778541955219794 1104 10-11 0.11233859235854737 1104 12-13 -0.12500830565304 1104 14-15 -0.06960362368550221 1104 16-17 0.039945168779780715 1104 18-19 0.1347030787715653 1104 20-21 -0.04993409671200055 1104 22-23 0.09422289882555646 1104 24-25 0.08297517410695576 1104 26-27 0.07177381591495902 1104 28-29 0.043078262543119195 1104 30-31 0.024322892727589362 1104 32-33 -0.027553326682550505 1104 34-35 -0.09327939301469712 1104 36-37 -0.09885251844647058 1104 38-39 -0.027487442612660118 1104 40-41 0.039035850179466536 1104 42-43 -0.08065103144944175 1104 44-45 -0.09944599583279512 1104 46-47 0.07757675047646018 1104 48-49 0.011366345591412141 1104 50-51 -0.30577726687896245 1104 52-53 -0.18257215721580167 1104 54-55 -0.2543410549863978 1104 56-57 0.46310068413430017 1104 58-59 -6.703236090580372 1104 60-61 -8.480937291479144 1104 62-63 -2.785830704531058 1104 64-65 -0.6824283749372277 1104 66-67 -1.8902190856678303 1104 68-69 -4.162905961106352 1104 70-71 -4.777898043308172 1104 72-73 -7.687475078389866 1104 74-75 -8.767284417126518 1104 76-77 -9.035434257823894 1104 78-79 -12.027309467432904 1104 80-81 -8.828239394982042 1104 82-83 -5.482952313643004 1104 84-85 -4.654802133185175 1104 86-87 -4.4070487614727405 1104 88-89 -4.558744766017366 1104 90-91 -9.523717767683905 1104 92-93 -14.329468196445276 1104 94-95 -11.86354559573645 1104 96-97 -11.864860001099187 1104 98-99 -11.362367900301614 1104 100-101 -10.88667131856084 1105 1 0.2373433206974802 1105 2 0.19258025562361425 1105 3 0.20471874709615534 1105 4 0.198754295086907 1105 5 0.2371475361393678 1105 6 0.2991546586192584 1105 7 0.1975011797503896 1105 8 0.25441837050561134 1105 9 0.3695005390342203 1105 10-11 0.2618643859005729 1105 12-13 0.3877454373238294 1105 14-15 0.2613224810742736 1105 16-17 0.30239071621519287 1105 18-19 0.47032802342287994 1105 20-21 0.5909530847431412 1105 22-23 0.47866081622493795 1105 24-25 0.4071511990943435 1105 26-27 0.36254031966320355 1105 28-29 0.6148147282958121 1105 30-31 0.6483858306850507 1105 32-33 0.5709536293294732 1105 34-35 0.5785183604260524 1105 36-37 0.23998792130674218 1105 38-39 0.3480711398424674 1105 40-41 0.3220584577456833 1105 42-43 0.5288409695232943 1105 44-45 0.4520437448050174 1105 46-47 0.6467274661789091 1105 48-49 0.711555339390955 1105 50-51 0.4627109514064145 1105 52-53 0.4940182653178269 1105 54-55 0.4929944768318961 1105 56-57 0.8295613482623061 1105 58-59 -6.247003371670171 1105 60-61 -8.281259965534069 1105 62-63 -2.3050913444813546 1105 64-65 0.22106214163645532 1105 66-67 -1.42769981026823 1105 68-69 -4.133849482252714 1105 70-71 -4.278407954061489 1105 72-73 -5.770456288653023 1105 74-75 -6.354505161040976 1105 76-77 -8.171589917306129 1105 78-79 -11.140355029768527 1105 80-81 -7.565927738654157 1105 82-83 -3.788487603965315 1105 84-85 -2.9376915524843277 1105 86-87 -2.781715439384854 1105 88-89 -3.1955695296169786 1105 90-91 -8.386952394103115 1105 92-93 -11.459951581999654 1105 94-95 -11.445745950249178 1105 96-97 -11.523047600463256 1105 98-99 -11.053527854139052 1105 100-101 -10.794300862600121 1106 1 0.1160630251005692 1106 2 0.22647982333867844 1106 3 0.14796405549559566 1106 4 0.1372500030998225 1106 5 0.07582288397868808 1106 6 0.1635531951080651 1106 7 0.10667877415176008 1106 8 0.07377605170452028 1106 9 0.23096396508923078 1106 10-11 0.17302887340559892 1106 12-13 0.08454150568061891 1106 14-15 0.2396986562358734 1106 16-17 0.2984297006116421 1106 18-19 0.20785646462169893 1106 20-21 0.15379829012636748 1106 22-23 0.4183308876764684 1106 24-25 0.4118603592355825 1106 26-27 0.3565647413144859 1106 28-29 0.5068291633349418 1106 30-31 0.41679252758967067 1106 32-33 0.4976455746968327 1106 34-35 0.46390698054103296 1106 36-37 0.18960629309733434 1106 38-39 0.4329573431206839 1106 40-41 0.3877791774117725 1106 42-43 0.18217548059008237 1106 44-45 0.4102418338970111 1106 46-47 0.4875477433435762 1106 48-49 0.6392529698054261 1106 50-51 0.5721055396502237 1106 52-53 0.6459708906346933 1106 54-55 0.2728819465283152 1106 56-57 1.272934894807964 1106 58-59 -6.584296301651079 1106 60-61 -8.691432070704249 1106 62-63 -2.3378192093002568 1106 64-65 0.16576702362543472 1106 66-67 -0.9866643484299829 1106 68-69 -3.931590256023595 1106 70-71 -4.238897302313726 1106 72-73 -5.916406730196648 1106 74-75 -6.634830023916617 1106 76-77 -8.497560642825412 1106 78-79 -11.675780684634447 1106 80-81 -7.891646776857417 1106 82-83 -4.081486758977032 1106 84-85 -3.094516163140547 1106 86-87 -3.0014964660988284 1106 88-89 -2.976365308506743 1106 90-91 -7.888056519220978 1106 92-93 -11.236629373281165 1106 94-95 -11.685426238183723 1106 96-97 -11.62628196871213 1106 98-99 -11.123894945144503 1106 100-101 -11.027725683119927 1107 1 0.01207042856279017 1107 2 0.19057641988493046 1107 3 0.0743159126612909 1107 4 0.1329214342276117 1107 5 0.11791419264017122 1107 6 0.18442943382736843 1107 7 -0.012119687978028537 1107 8 0.12142631011627003 1107 9 0.11188279065785878 1107 10-11 0.20788772373008868 1107 12-13 0.290117291107272 1107 14-15 0.2315862955669843 1107 16-17 0.10971341564187753 1107 18-19 0.13450797565013062 1107 20-21 0.09632086740371193 1107 22-23 0.20587153418761517 1107 24-25 0.2592767182187927 1107 26-27 0.44609453887341743 1107 28-29 0.2810701549245138 1107 30-31 0.33096975537849715 1107 32-33 0.22657583158384398 1107 34-35 0.13154478690534432 1107 36-37 0.1452739335508184 1107 38-39 0.23341857712282632 1107 40-41 0.3352328078294988 1107 42-43 0.20715369480129908 1107 44-45 0.426837681308605 1107 46-47 0.2002696910301509 1107 48-49 0.1354008823403774 1107 50-51 0.28752013671107335 1107 52-53 0.575721648504846 1107 54-55 0.49112419602880664 1107 56-57 1.2476369086301595 1107 58-59 -6.090589562192168 1107 60-61 -8.53438750486881 1107 62-63 -2.3758662496624687 1107 64-65 -0.19217269194693287 1107 66-67 -1.6788398884157765 1107 68-69 -3.99017046849195 1107 70-71 -4.764166699607101 1107 72-73 -6.4808206633600705 1107 74-75 -6.914813145886328 1107 76-77 -8.86268093320377 1107 78-79 -12.03740413090799 1107 80-81 -8.51571671162182 1107 82-83 -4.648419690513009 1107 84-85 -3.695366100086172 1107 86-87 -3.4490469810693476 1107 88-89 -3.491796423589708 1107 90-91 -8.365172705506147 1107 92-93 -11.710559487223751 1107 94-95 -11.527280470873949 1107 96-97 -11.949800138007033 1107 98-99 -11.20792350014581 1107 100-101 -10.550461787598534 1108 1 -0.0786524635328334 1108 2 -0.008788986332714188 1108 3 0.0032891536882999617 1108 4 -0.03059594057815218 1108 5 -0.10128721216563719 1108 6 -0.0057129067443071335 1108 7 0.024810653161893015 1108 8 0.018033510771218175 1108 9 0.001970503147184388 1108 10-11 0.22094683775925006 1108 12-13 0.05417486881315625 1108 14-15 0.10462067318030677 1108 16-17 0.052066143164914536 1108 18-19 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4.514384363882183 2311 60-61 4.101137698490582 2311 62-63 1.5828274272849683 2311 64-65 1.3545468594064296 2311 66-67 -0.4340331426397839 2311 68-69 -3.0823446305450215 2311 70-71 -0.7339563283138251 2311 72-73 -1.1204363797722188 2311 74-75 1.3956528116001827 2311 76-77 4.397627346290339 2311 78-79 5.139548686267357 2311 80-81 3.030634661260436 2311 82-83 1.743029741286417 2311 84-85 1.3757081341672013 2311 86-87 1.3745101253493068 2311 88-89 1.4921178374768154 2311 90-91 2.9594404917901436 2311 92-93 4.8977266096969 2311 94-95 8.724766400470283 2311 96-97 9.589265176710201 2311 98-99 10.130674737477866 2311 100-101 9.734959421490954 2312 1 -0.28186455334320826 2312 2 -0.3299171063078674 2312 3 -0.1954168633017943 2312 4 -0.1242654979848723 2312 5 -0.2623248501573556 2312 6 -0.2166737524789113 2312 7 -0.15503258968602296 2312 8 -0.1528691768032715 2312 9 -0.21127402379499927 2312 10-11 -0.28506804067618674 2312 12-13 -0.32340225564386316 2312 14-15 -0.49874860641458696 2312 16-17 -0.3493446506022906 2312 18-19 -0.3977221236323416 2312 20-21 -0.3632503337329851 2312 22-23 -0.4791613988312733 2312 24-25 -0.5058292496868262 2312 26-27 -0.6234293306721526 2312 28-29 -0.746702510204301 2312 30-31 -0.5052120197029879 2312 32-33 -0.3586790421233772 2312 34-35 -0.26458926269221195 2312 36-37 -0.30414844056283385 2312 38-39 -0.3533556485376437 2312 40-41 -0.41767250965167335 2312 42-43 -0.3979941976215642 2312 44-45 -0.5465659606958368 2312 46-47 -0.5421262084741798 2312 48-49 -0.4486171679762023 2312 50-51 -0.28544563973399306 2312 52-53 -0.5054868718977232 2312 54-55 -0.23007275108714964 2312 56-57 0.7950472783236187 2312 58-59 4.213191665449937 2312 60-61 4.048152015111938 2312 62-63 1.357741185314783 2312 64-65 1.1710054213638514 2312 66-67 -0.6505050308366691 2312 68-69 -1.4497846397522416 2312 70-71 -0.05792049082529438 2312 72-73 6.615523366908082 2312 74-75 5.376128608827315 2312 76-77 5.3175490142509325 2312 78-79 5.427181683651575 2312 80-81 3.0448758177357256 2312 82-83 1.4799281297200508 2312 84-85 1.4875935244695349 2312 86-87 1.2190353124466782 2312 88-89 1.4253331864779035 2312 90-91 2.850150941883882 2312 92-93 4.790711354181401 2312 94-95 8.707170968947466 2312 96-97 9.349744951617902 2312 98-99 9.547313304624447 2312 100-101 8.879742198739832 2313 1 -0.28764577629972976 2313 2 -0.27196815517226014 2313 3 -0.29191668868735476 2313 4 -0.3208915987983403 2313 5 -0.3088982593993279 2313 6 -0.36636902211740363 2313 7 -0.5014471561147786 2313 8 -0.5302934243481658 2313 9 -0.2343487331720553 2313 10-11 -0.47642549640553966 2313 12-13 -0.5198732005179352 2313 14-15 -0.4125482166300998 2313 16-17 -0.5965677177331017 2313 18-19 -0.5630871190186326 2313 20-21 -0.5738681206460186 2313 22-23 -0.6145818099791498 2313 24-25 -0.5340384429066631 2313 26-27 -0.4475762034350623 2313 28-29 -0.6140956446307939 2313 30-31 -0.5206231522184552 2313 32-33 -0.48515878428801074 2313 34-35 -0.7783105780458897 2313 36-37 -0.7935077131016683 2313 38-39 -1.0081014232266412 2313 40-41 -1.2154866543430458 2313 42-43 -1.1323185831169909 2313 44-45 -1.2585166549034525 2313 46-47 -1.0492843763846622 2313 48-49 -1.259242175017107 2313 50-51 -1.0756207867224887 2313 52-53 -0.8527030773467246 2313 54-55 -0.9945782952236328 2313 56-57 -0.014211793178180443 2313 58-59 3.4622223248780415 2313 60-61 3.2674764569346024 2313 62-63 1.017755349054994 2313 64-65 1.070082627573779 2313 66-67 1.473898028449284 2313 68-69 -0.13790993611032576 2313 70-71 2.2862000207898934 2313 72-73 7.602784961870174 2313 74-75 8.886680236838135 2313 76-77 7.908716186326547 2313 78-79 5.793589603466167 2313 80-81 2.954801138058489 2313 82-83 1.4193653386746448 2313 84-85 1.1175478891648503 2313 86-87 0.9844861300621517 2313 88-89 0.958926751654154 2313 90-91 2.4649189709528336 2313 92-93 4.455853088876008 2313 94-95 8.125641643450788 2313 96-97 8.75369036745118 2313 98-99 9.307232019298306 2313 100-101 8.74181880992929 2314 1 -0.3890830816844044 2314 2 -0.3151063035735646 2314 3 -0.3319125115779116 2314 4 -0.22197954593891467 2314 5 -0.49241576528525144 2314 6 -0.40166559688766057 2314 7 -0.45491035957798687 2314 8 -0.4028536126978324 2314 9 -0.6537874815581688 2314 10-11 -0.48294748319068503 2314 12-13 -0.4701940784703851 2314 14-15 -0.5851672642491437 2314 16-17 -0.6110309591700229 2314 18-19 -0.6936645094644476 2314 20-21 -0.7779009299419855 2314 22-23 -0.7437304391277806 2314 24-25 -0.675182021550242 2314 26-27 -0.6675105088956812 2314 28-29 -0.8233450940118132 2314 30-31 -0.8919159675902151 2314 32-33 -0.6779276382410728 2314 34-35 -0.9727550224903325 2314 36-37 -0.9884648027955976 2314 38-39 -0.8409790718937629 2314 40-41 -0.9087444199692314 2314 42-43 -0.8970476406092018 2314 44-45 -0.6825407302433177 2314 46-47 -0.6469053226898183 2314 48-49 -0.6538223755183594 2314 50-51 -0.6302839598067109 2314 52-53 -0.7745056899388061 2314 54-55 -0.7377097982235554 2314 56-57 0.5729853663874032 2314 58-59 3.76839495341909 2314 60-61 3.4037593991386004 2314 62-63 1.2383019807858382 2314 64-65 1.0821725113741856 2314 66-67 1.3623454728177826 2314 68-69 -0.6259672004833803 2314 70-71 2.465042012131887 2314 72-73 8.152283708737343 2314 74-75 9.647569201674465 2314 76-77 8.481583012482847 2314 78-79 6.33753944017916 2314 80-81 3.402345187576227 2314 82-83 1.8491424329254258 2314 84-85 1.431856387683876 2314 86-87 1.3363601332519472 2314 88-89 1.510827336449477 2314 90-91 2.7061707747046384 2314 92-93 4.693245813110838 2314 94-95 8.57115884554694 2314 96-97 9.214117002219924 2314 98-99 9.479319434806774 2314 100-101 8.567571638971591 2315 1 -0.7110478447134021 2315 2 -0.461501874868393 2315 3 -0.4616953503453658 2315 4 -0.23818183627419387 2315 5 -0.7811347107541096 2315 6 -0.6005604558447999 2315 7 -0.6474213160100533 2315 8 -0.6552653845869472 2315 9 -0.9726264389671613 2315 10-11 -0.7884368931037145 2315 12-13 -0.5777757259378546 2315 14-15 -0.6330855421558255 2315 16-17 -0.7149432195192702 2315 18-19 -0.5844877343867623 2315 20-21 -0.778253706702138 2315 22-23 -0.6382908928529645 2315 24-25 -0.7192816606324755 2315 26-27 -0.7079492410702812 2315 28-29 -0.7369173318376312 2315 30-31 -1.0254292509920688 2315 32-33 -0.8739756905063061 2315 34-35 -0.7949385749523046 2315 36-37 -0.7655031062107511 2315 38-39 -0.8207690340381006 2315 40-41 -0.7274968792302943 2315 42-43 -0.6430339569414087 2315 44-45 -0.4686240262637327 2315 46-47 -0.4372592527586079 2315 48-49 -0.5883678701520836 2315 50-51 -0.37797395044455584 2315 52-53 -0.4849929967596083 2315 54-55 -0.49177097107060774 2315 56-57 0.9280229506807025 2315 58-59 4.510130140187247 2315 60-61 3.9583744993407706 2315 62-63 1.5714987827288205 2315 64-65 1.5391091540248958 2315 66-67 2.506932215195768 2315 68-69 6.12523359733483 2315 70-71 4.7129635125185825 2315 72-73 9.078634775887043 2315 74-75 10.490286736036282 2315 76-77 9.227565936730823 2315 78-79 6.849895955016137 2315 80-81 3.908712977701189 2315 82-83 2.343893182576039 2315 84-85 1.7919091685145254 2315 86-87 1.7680995262045478 2315 88-89 1.9082693657299465 2315 90-91 3.127848397803376 2315 92-93 5.178765005523427 2315 94-95 8.684676640420822 2315 96-97 9.642163263464683 2315 98-99 10.074876143670968 2315 100-101 9.185493207199974 2316 1 -0.4870127176021555 2316 2 -0.4724755330989936 2316 3 -0.5865915478783421 2316 4 -0.40553992538215056 2316 5 -0.5627966907171142 2316 6 -0.5842389657938014 2316 7 -0.532611332440247 2316 8 -0.5285711323032984 2316 9 -0.7003218281774082 2316 10-11 -0.5416864003761148 2316 12-13 -0.6265156619991075 2316 14-15 -0.6954463221088503 2316 16-17 -0.9634391975078032 2316 18-19 -1.1806800995148379 2316 20-21 -0.8754903335585524 2316 22-23 -0.8294959281190728 2316 24-25 -2.0280686062916615 2316 26-27 -1.528395921351553 2316 28-29 -1.1192276094401734 2316 30-31 -0.7736953488390341 2316 32-33 -0.7187504614280655 2316 34-35 -0.8074964459414318 2316 36-37 -0.9267176930858412 2316 38-39 -1.0872803088571388 2316 40-41 -0.9409789040288459 2316 42-43 -0.9768119034167668 2316 44-45 -1.0750576407992796 2316 46-47 -1.0610960414221253 2316 48-49 -1.0347158053294478 2316 50-51 -1.1319211172163648 2316 52-53 -1.1025401578289618 2316 54-55 -1.1096460231945855 2316 56-57 0.32885016278700974 2316 58-59 4.020803528620537 2316 60-61 3.6970952252630482 2316 62-63 1.1699338157495909 2316 64-65 0.6362745002172652 2316 66-67 6.006935779228968 2316 68-69 8.799936267864908 2316 70-71 5.467634178030503 2316 72-73 9.077567347942885 2316 74-75 10.090608924637792 2316 76-77 8.69687426480721 2316 78-79 6.191241234988766 2316 80-81 3.235360184746572 2316 82-83 1.8857224582575718 2316 84-85 1.5128720990051896 2316 86-87 1.3405001876891127 2316 88-89 1.5403399620996225 2316 90-91 2.909351778288869 2316 92-93 4.6039702047350595 2316 94-95 8.709626654855153 2316 96-97 9.479392085088383 2316 98-99 9.797712864617862 2316 100-101 8.789519317307215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 40.0 5 81.0 6 208.0 7 451.0 8 1008.0 9 1654.0 10 2614.0 11 4038.0 12 5521.0 13 7372.0 14 9489.0 15 13403.0 16 17446.0 17 22489.0 18 23801.0 19 23063.0 20 24284.0 21 23969.0 22 22750.0 23 22394.0 24 22972.0 25 23780.0 26 31578.0 27 45614.0 28 62946.0 29 80710.0 30 94923.0 31 101341.0 32 105489.0 33 108434.0 34 108667.0 35 101873.0 36 81267.0 37 46531.0 38 12984.0 39 496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13483089682551 19.53502302422223 14.881589145280014 22.448556933672236 2 16.38393916659897 21.34680231215346 38.04508667389812 24.22417184734945 3 19.403940757235112 25.638495532582006 29.825022478451345 25.13254123173153 4 13.589808320677617 17.389230312234456 35.72495144107906 33.29600992600887 5 13.758832355966955 38.032979441438904 33.06255543257129 15.145632770022862 6 31.641006078744145 36.04767748650738 16.963622303965877 15.34769413078259 7 28.675207515224688 33.281277002064996 20.09315986178128 17.950355620929034 8 27.266406941851795 34.087368836844824 18.929383732264704 19.716840489038677 9 27.228036196803195 16.558213764373562 19.546369289896955 36.667380748926284 10-11 25.81144565024357 26.598502338553036 26.0048053223285 21.58524668887489 12-13 26.922069203331073 24.013232554824395 26.81081571819819 22.253882523646343 14-15 23.58969744076554 25.176476959244653 25.722591917737525 25.511233682252275 16-17 22.466275540718218 28.593499515406258 26.178016725452363 22.76220821842316 18-19 22.321376308805405 26.692164190247897 29.038387643324022 21.94807185762268 20-21 23.390985521119447 25.62887684947969 28.866655889068248 22.113481740332613 22-23 22.968270060070513 25.68669362128103 28.960349321608582 22.384686997039875 24-25 22.912245130370447 26.06142753272899 28.951191017535365 22.075136319365193 26-27 22.6482342967748 26.06520162826877 29.232226597419185 22.054337477537242 28-29 22.209763165994847 26.115212733297042 29.5399223600815 22.135101740626606 30-31 22.304211254010074 26.106891751115814 29.28468574086404 22.304211254010074 32-33 22.255028522235236 26.31941717959969 28.968922987973915 22.456631310191156 34-35 22.817079192150867 26.081366271661572 28.817732224770644 22.28382231141692 36-37 22.843895909098695 25.878864904628745 28.918334516902604 22.35890466936996 38-39 22.53937807572762 25.98367524458457 29.16040701166327 22.31653966802454 40-41 22.260422786247 26.01658883390256 29.397524619382846 22.325463760467596 42-43 22.428294387619154 26.140102608139426 28.717334530335357 22.71426847390606 44-45 22.39830899344962 26.257958954775056 28.477823263209295 22.86590878856603 46-47 22.532376026326208 26.5096664056457 28.123986817777357 22.83397075025073 48-49 22.647322406548103 26.417461613526378 27.99478222770996 22.94043375221556 50-51 22.43955809361157 26.61172442744382 27.94442953084288 23.00428794810173 52-53 22.64938083485438 26.711699996933348 27.68534231610339 22.95357685210888 54-55 22.32157186965974 26.44216619932242 27.82687514162701 23.409386789390826 56-57 22.46752040056611 26.74634548427991 27.669280932633768 23.116853182520213 58-59 21.17612266582775 26.037797780509962 29.498367644367974 23.287711909294316 60-61 22.628894061710664 26.242888479536553 28.248772608683844 22.87944485006894 62-63 22.480756588312637 26.3839508094955 27.90888989674597 23.226402705445892 64-65 21.776068489228372 26.420418518698714 28.402348694332364 23.401164297740554 66-67 21.779669405127187 26.168141610796553 28.956806121438976 23.095382862637283 68-69 22.266061326703422 25.540523055656557 29.388346223012746 22.80506939462728 70-71 21.552867440797023 25.97947497201558 29.438996413026793 23.0286611741606 72-73 21.41217152725494 25.88559032252173 29.391098944138577 23.31113920608475 74-75 21.76739274675125 26.268630751055944 28.632652565824607 23.331323936368197 76-77 20.36330115471711 26.55937955450383 29.307858382944996 23.76946090783406 78-79 21.318161915101637 26.544063692764787 28.276620460620894 23.861153931512682 80-81 21.816437755064253 27.097484766325408 27.37705250944004 23.7090249691703 82-83 22.107911317177788 26.83432236577782 27.006379368768606 24.051386948275784 84-85 21.92848399805366 26.502897607771338 27.020620518901545 24.547997875273456 86-87 21.996534850003442 26.506048084431473 27.208276539835484 24.289140525729593 88-89 22.001232787365684 26.377428841968285 27.089108705883852 24.53222966478218 90-91 21.733624874140332 26.555781124102918 27.369344396346506 24.34124960541024 92-93 21.615426928675227 26.414063992730586 27.47256517006184 24.497943908532353 94-95 20.25490915986492 26.2662545944572 28.72908538528886 24.74975086038902 96-97 19.929062909170998 26.243093254078705 28.92632723676111 24.90151659998919 98-99 20.456600943681646 26.19790761446807 28.207214079266684 25.1382773625836 100-101 20.340221869920814 26.248223221372125 28.16560476446563 25.245950144241437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1424.0 1 988.5 2 577.5 3 645.0 4 705.5 5 808.0 6 1116.0 7 1743.5 8 2284.5 9 2553.5 10 2696.5 11 2712.0 12 2817.0 13 2911.5 14 2927.0 15 2906.0 16 2796.5 17 2695.5 18 2734.5 19 2803.0 20 2833.5 21 3064.5 22 3364.5 23 3728.0 24 4206.0 25 4774.0 26 5513.0 27 6395.0 28 7516.5 29 8843.0 30 10364.0 31 12343.0 32 14744.5 33 17203.5 34 19825.5 35 22541.5 36 25278.0 37 27816.5 38 29754.0 39 31278.0 40 32891.0 41 34883.5 42 37292.5 43 39737.0 44 42180.0 45 44374.0 46 47106.5 47 50089.5 48 52543.5 49 53063.0 50 51397.5 51 49416.5 52 47659.0 53 46570.5 54 44752.5 55 42083.0 56 39092.5 57 35444.5 58 31330.5 59 26864.0 60 22668.5 61 18635.5 62 14917.5 63 11923.0 64 9388.5 65 7336.0 66 5611.0 67 4239.5 68 3075.0 69 2164.0 70 1537.0 71 1133.5 72 869.0 73 650.0 74 485.5 75 323.0 76 195.0 77 112.0 78 56.5 79 34.0 80 19.5 81 9.0 82 6.0 83 5.5 84 3.0 85 0.0 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02182065492226989 2 0.005176432737034828 3 0.00350404677583896 4 0.0 5 0.06275429225820683 6 3.185497068944509E-4 7 0.0 8 2.3891228017083822E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.3891228017083822E-4 16-17 0.0 18-19 0.001075105260768772 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.1945614008541911E-4 28-29 0.0017918421012812866 30-31 0.00820265495253211 32-33 3.185497068944509E-4 34-35 8.7601169395974E-4 36-37 0.024488508717510918 38-39 0.023652315736912982 40-41 0.026160894678706782 42-43 0.026479444385601233 44-45 0.027395274792922784 46-47 0.028988023327395036 48-49 0.029147298180842263 50-51 0.024846877137767176 52-53 0.019949175394264988 54-55 0.01533020464429545 56-57 0.14856361955289957 58-59 0.7676251561889031 60-61 0.30652445545918544 62-63 1.1945614008541911E-4 64-65 0.15035546165418084 66-67 1.4393270318892148 68-69 2.7521898301413326 70-71 2.2119693459617054 72-73 1.8920658028129533 74-75 1.5792898093559642 76-77 1.1253962957447334 78-79 0.42315346689591626 80-81 1.9909356680903182E-4 82-83 1.9909356680903182E-4 84-85 0.0 86-87 0.0036235029159243795 88-89 0.0 90-91 0.22358207552654275 92-93 0.17683490603978208 94-95 1.3081243713620627 96-97 1.2855073421725567 98-99 1.0272431673078806 100-101 0.4128006014218466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1255691.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.25052496505877 #Duplication Level Percentage of deduplicated Percentage of total 1 74.71110149272693 37.54272070727314 2 13.696285822415444 13.764911052957354 3 4.839712073112645 7.2959421706093 4 2.184110703944102 4.390108378199808 5 1.203369196685143 3.023496693010475 6 0.7031933759702269 2.120150177667351 7 0.4699305659362177 1.6529980344795474 8 0.33030587674603173 1.3278434964425676 9 0.24136592443523347 1.091588797039247 >10 1.4138710369912972 13.343320942539924 >50 0.13360173981051607 4.633610451965497 >100 0.06792704176318896 5.765408834882894 >500 0.003641770837917207 1.3485574753110827 >1k 0.0011083650376269758 1.032204621347022 >5k 4.7501358755441825E-4 1.6671381662748903 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8117 0.6464169927155646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7431 0.5917857179831663 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5405 0.4304402914411269 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3209 0.2555565023560733 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2118 0.16867206980061178 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1784 0.1420731692749251 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1591 0.12670314591726786 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1552 0.12359728627504697 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1514 0.12057106405954968 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 7.963742672361274E-5 0.0 0.0 0.0 20-21 0.0 7.963742672361274E-5 0.0 3.5836842025625733E-4 0.0 22-23 0.0 7.963742672361274E-5 0.0 0.0011547426874923847 0.0 24-25 0.0 7.963742672361274E-5 0.0 0.002707672508602833 0.0 26-27 0.0 7.963742672361274E-5 0.0 0.0065302689913362445 0.0 28-29 0.0 7.963742672361274E-5 0.0 0.014056005816717648 0.0 30-31 0.0 7.963742672361274E-5 0.0 0.03492101161830419 0.0 32-33 0.0 7.963742672361274E-5 0.0 0.07788540333569326 0.0 34-35 0.0 7.963742672361274E-5 0.0 0.1431084558223321 0.0 36-37 0.0 7.963742672361274E-5 0.0 0.24647783570958143 0.0 38-39 0.0 7.963742672361274E-5 0.0 0.3945636307021393 0.0 40-41 0.0 7.963742672361274E-5 0.0 0.5567850689381384 0.0 42-43 0.0 7.963742672361274E-5 0.0 0.7125956943228867 0.0 44-45 0.0 7.963742672361274E-5 0.0 0.9032476938992156 0.0 46-47 0.0 7.963742672361274E-5 0.0 1.1255555705981806 0.0 48-49 0.0 7.963742672361274E-5 0.0 1.3417313654394274 0.0 50-51 7.963742672361274E-5 7.963742672361274E-5 0.0 1.5567524175931817 0.0 52-53 7.963742672361274E-5 7.963742672361274E-5 0.0 1.762137341113379 0.0 54-55 7.963742672361274E-5 7.963742672361274E-5 0.0 1.9828922879912334 0.0 56-57 7.963742672361274E-5 7.963742672361274E-5 0.0 2.179118907438215 0.0 58-59 7.963742672361274E-5 7.963742672361274E-5 0.0 2.315936006549382 0.0 60-61 7.963742672361274E-5 1.5927485344722548E-4 0.0 2.4196637548568876 0.0 62-63 7.963742672361274E-5 1.5927485344722548E-4 0.0 2.5068667371192435 0.0 64-65 7.963742672361274E-5 1.5927485344722548E-4 0.0 2.593074251547554 0.0 66-67 7.963742672361274E-5 1.5927485344722548E-4 0.0 2.6657832221462128 0.0 68-69 7.963742672361274E-5 1.5927485344722548E-4 0.0 2.7538223973891665 0.0 70-71 7.963742672361274E-5 1.5927485344722548E-4 0.0 2.9164818414721454 0.0 72-73 7.963742672361274E-5 1.5927485344722548E-4 0.0 3.1194378234772726 0.0 74-75 7.963742672361274E-5 1.5927485344722548E-4 0.0 3.356438805406744 0.0 76-77 7.963742672361274E-5 1.5927485344722548E-4 0.0 3.6832708046804505 0.0 78-79 7.963742672361274E-5 1.5927485344722548E-4 0.0 4.151140686681676 0.0 80-81 7.963742672361274E-5 1.5927485344722548E-4 0.0 4.680251749833358 0.0 82-83 7.963742672361274E-5 1.5927485344722548E-4 0.0 5.1734861522460545 0.0 84-85 7.963742672361274E-5 1.5927485344722548E-4 0.0 5.576889537314514 0.0 86-87 7.963742672361274E-5 1.5927485344722548E-4 0.0 5.937447986805671 0.0 88-89 7.963742672361274E-5 1.9909356680903185E-4 0.0 6.29983809711147 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACCG 85 6.682559E-4 27.741032 5 GGTATCA 7810 0.0 26.880434 1 GTATCAA 10505 0.0 24.654919 1 TACATGG 7945 0.0 24.045649 2 GTACATG 7975 0.0 23.839748 1 ACATGGG 7855 0.0 23.177393 3 GAGTACT 6115 0.0 22.58128 12-13 GGACCGT 210 3.7480277E-8 22.44093 6 ATGGGAG 1750 0.0 22.097712 5 GTACTTT 6365 0.0 21.656897 14-15 CATGGGA 4950 0.0 21.531525 4 AGTACTT 6325 0.0 20.676634 12-13 ATCAACG 12590 0.0 20.56696 3 TCAACGC 12655 0.0 20.421608 4 TATCAAC 12790 0.0 20.210888 2 CAACGCA 12845 0.0 20.082823 5 AACGCAG 12875 0.0 20.021666 6 ACTTTTT 7115 0.0 19.837276 16-17 TACACCG 145 6.451301E-4 19.51438 5 TACCTGG 895 0.0 19.500874 2 >>END_MODULE