FastQCFastQC Report
Fri 27 May 2016
SRR939820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356123
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82710.60990042938583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64550.4759892723595131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44350.3270352320549095No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29010.21391864897210652No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19730.14548827798068464No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16300.12019558697846729No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.10824976790453372No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14320.1055951414436596No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13640.10058084701756403No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG81700.026.3118061
TACATGG83050.025.4885672
GTATCAA120950.024.7736551
ACATGGG84400.024.5253313
GAGTACT63450.022.7249812-13
TACCTGG7850.022.171992
GTACTTT66400.021.65010614-15
CATGGGA51700.021.474654
CATGGGG33400.021.4092644
TATCAAC139500.021.2778472
GGTATCA90450.021.1141681
AGTACTT65200.021.06880412-13
ATCAACG141750.020.8752773
TCAACGC142850.020.6486634
CAACGCA143850.020.505125
AACGCAG146350.020.2520286
TACACCG1908.970261E-619.8080185
ACTTTTT76100.019.69762816-17
CGTACAC1708.5670064E-519.3710773
TACTTTT72450.018.99784914-15
Produced by FastQC (version 0.11.5)