FastQCFastQC Report
Fri 27 May 2016
SRR939820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356123
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88020.6490561696837234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79740.5879997610836185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55940.4124994561702736No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34450.25403300438087106No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22750.16775764440246202No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18280.134796032513275No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17660.13022417583065843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17520.12919182109587404No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15490.11422267744150051No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13770.10153946212843525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89700.026.9289991
GTACATG85600.024.9478821
TACCTGG8100.023.4326732
TACATGG89700.023.2230322
GAGTACT65300.022.84347212-13
GTATCAA116350.022.392411
ACATGGG89400.022.3887353
CGTATAG1100.002892998321.5710961
GTACTTT69550.021.31118214-15
AGTACTT68950.020.25842512-13
ATGGGAG19000.020.2202325
CATGGGG35100.019.8639164
CATGGGA54850.019.715734
ACTTTTT77200.019.69089316-17
GTACTAC2201.3569952E-619.4139861
GTATACC1507.7988155E-418.9720673
ATCAACG137400.018.9168363
TATCAAC137900.018.8221782
TCAACGC138400.018.8144234
CAACGCA140700.018.5068665
Produced by FastQC (version 0.11.5)