##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939820_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1356123 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36690034753485 31.0 31.0 34.0 30.0 34.0 2 31.585238212168072 31.0 31.0 34.0 30.0 34.0 3 31.6109608051777 31.0 31.0 34.0 30.0 34.0 4 35.4348639467069 37.0 35.0 37.0 33.0 37.0 5 35.167227456506524 37.0 35.0 37.0 32.0 37.0 6 35.20407293438722 37.0 35.0 37.0 32.0 37.0 7 35.108128097525075 37.0 35.0 37.0 32.0 37.0 8 35.07787051764478 37.0 35.0 37.0 32.0 37.0 9 36.629530654667754 39.0 35.0 39.0 32.0 39.0 10-11 36.49565747354775 38.0 35.0 39.0 32.0 39.0 12-13 36.40382730769997 38.0 35.0 39.0 32.0 39.0 14-15 37.37452502464747 39.0 36.0 41.0 32.0 41.0 16-17 37.30174880892072 39.0 36.0 41.0 32.0 41.0 18-19 37.30795252348054 39.0 36.0 40.5 32.0 41.0 20-21 37.23271561650381 39.0 36.0 40.0 31.0 41.0 22-23 37.10101406730806 39.0 36.0 40.0 31.0 41.0 24-25 36.92942269985835 39.0 36.0 40.0 31.0 41.0 26-27 36.6004344738641 38.0 35.0 40.0 30.0 41.0 28-29 36.35362869002296 38.0 35.0 40.0 30.0 41.0 30-31 36.055290707406336 38.0 34.5 40.0 29.5 41.0 32-33 35.99127918337791 38.0 34.5 40.0 30.0 41.0 34-35 35.80162087067323 38.0 34.0 40.0 29.0 41.0 36-37 35.80233024585528 38.0 34.5 40.0 29.0 41.0 38-39 35.683370903671715 38.0 34.0 40.0 28.5 41.0 40-41 35.569177722079786 38.0 34.0 40.0 28.0 41.0 42-43 35.30128129970512 38.0 34.0 40.0 27.0 41.0 44-45 34.99042933421231 38.0 33.5 40.0 26.0 41.0 46-47 34.624209972104296 38.0 33.0 40.0 24.5 41.0 48-49 34.260858712668394 38.0 33.0 40.0 24.0 41.0 50-51 34.000530925292175 37.0 33.0 40.0 23.5 41.0 52-53 33.71496943861287 37.0 33.0 40.0 23.0 41.0 54-55 33.4184413950652 37.0 32.0 40.0 22.5 41.0 56-57 33.046620402426626 36.0 31.5 39.5 21.5 41.0 58-59 32.654094429487586 36.0 31.0 39.0 20.0 40.5 60-61 32.30847054433853 35.0 31.0 39.0 19.0 40.0 62-63 31.987943571490195 35.0 31.0 39.0 18.0 40.0 64-65 31.519895319229892 35.0 30.0 38.0 15.5 40.0 66-67 30.580574180955566 34.5 28.5 37.5 10.0 40.0 68-69 28.671448681277436 33.0 25.5 36.5 7.0 39.0 70-71 28.00080855497621 33.0 24.0 36.0 6.5 39.0 72-73 28.560788365067182 33.0 26.0 35.5 6.0 38.5 74-75 28.674991870206462 33.0 26.0 35.0 4.0 38.0 76-77 28.24894497033086 32.5 26.5 35.0 2.0 37.0 78-79 28.781736243688812 33.0 28.0 35.0 2.0 37.0 80-81 28.61249753894005 33.0 28.0 35.0 2.0 36.5 82-83 28.182493402147152 33.0 26.5 35.0 2.0 36.0 84-85 27.83257197171643 33.0 26.0 35.0 2.0 36.0 86-87 27.450882405209555 33.0 26.0 35.0 2.0 35.0 88-89 26.99087177195579 32.5 25.5 35.0 2.0 35.0 90-91 26.502766710689222 32.0 25.0 34.0 2.0 35.0 92-93 26.01331553258812 32.0 24.0 34.0 2.0 35.0 94-95 25.19533368285915 31.5 20.0 34.0 2.0 35.0 96-97 24.65048450619892 31.0 18.5 34.0 2.0 35.0 98-99 23.978295110399277 31.0 7.0 34.0 2.0 35.0 100-101 22.539246808733424 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2037809482852957 1101 2 0.2945174364904979 1101 3 0.3167011937619364 1101 4 0.2702407859412972 1101 5 0.28948080604595106 1101 6 0.1892483317521183 1101 7 0.2465655692612927 1101 8 0.22970183378202336 1101 9 0.33769043141964517 1101 10-11 0.36377648423707143 1101 12-13 0.3655028661502726 1101 14-15 0.44679393552559077 1101 16-17 0.2834531013978179 1101 18-19 0.2787586226492422 1101 20-21 0.32544083831088244 1101 22-23 0.2702179655688397 1101 24-25 0.17316664556658878 1101 26-27 0.37529755495941686 1101 28-29 0.23269822628959957 1101 30-31 -0.09921981049397743 1101 32-33 0.11774829870407899 1101 34-35 0.19468491226951556 1101 36-37 0.34534803349465193 1101 38-39 0.2891030778615402 1101 40-41 0.44674773826778136 1101 42-43 0.18869451489850775 1101 44-45 0.06070078370094478 1101 46-47 0.2858802364540196 1101 48-49 0.338907583431741 1101 50-51 0.32780733893073943 1101 52-53 -0.05227900180656064 1101 54-55 -0.31735987538707633 1101 56-57 0.07564227979151639 1101 58-59 0.09987176039533807 1101 60-61 -0.12120091133508026 1101 62-63 -0.0678733145110968 1101 64-65 -0.03529824994828701 1101 66-67 -0.05280197103587625 1101 68-69 -8.311444152747093 1101 70-71 -6.405045483265962 1101 72-73 -1.744925453599091 1101 74-75 -0.28972760984274615 1101 76-77 -0.15501254463259428 1101 78-79 -0.5173960046975843 1101 80-81 -0.6866903885809137 1101 82-83 -0.28953672101735606 1101 84-85 -0.2291498473689444 1101 86-87 -0.43240953251762804 1101 88-89 -0.10887977635717405 1101 90-91 -0.45046408166598084 1101 92-93 -0.8731731286348676 1101 94-95 -0.6966279347290545 1101 96-97 -1.053184126406233 1101 98-99 -1.0797089871639827 1101 100-101 -1.1274232879603936 1102 1 0.23195456291791317 1102 2 0.1717642131995305 1102 3 0.2070079823065889 1102 4 0.26970747734111455 1102 5 0.41686645470143446 1102 6 0.3270672709619973 1102 7 0.39096013294125953 1102 8 0.3511963882878746 1102 9 0.45249745738239966 1102 10-11 0.4717190522439907 1102 12-13 0.3970328468644624 1102 14-15 0.5446205265545103 1102 16-17 0.5498162996767135 1102 18-19 0.4786659237347095 1102 20-21 0.3915862269053392 1102 22-23 0.5176189682696517 1102 24-25 0.3852505716050558 1102 26-27 0.32566078953152555 1102 28-29 0.294980404003347 1102 30-31 0.07453545832279218 1102 32-33 0.12479019014502768 1102 34-35 0.2910212132450951 1102 36-37 0.12929882191429698 1102 38-39 0.49433766811620217 1102 40-41 0.43088810831741853 1102 42-43 0.26067630578709355 1102 44-45 0.2937707560072127 1102 46-47 0.32731851631703535 1102 48-49 0.338233890667901 1102 50-51 0.3475949530653395 1102 52-53 0.2503922794749158 1102 54-55 0.20943409308420513 1102 56-57 -0.05157677293546925 1102 58-59 -0.054815961711561556 1102 60-61 0.012175889841628873 1102 62-63 0.11990827293386985 1102 64-65 0.0797126733006408 1102 66-67 0.5489163109644046 1102 68-69 -4.4883808745223135 1102 70-71 0.24247004468434952 1102 72-73 0.47315945437240714 1102 74-75 0.32845443288041665 1102 76-77 0.007975485664172055 1102 78-79 -0.12900746367466098 1102 80-81 -0.1907327972001518 1102 82-83 0.034725997485427484 1102 84-85 0.10108864944682949 1102 86-87 -0.03330945297500065 1102 88-89 -0.33855809751379695 1102 90-91 -0.2296163528649977 1102 92-93 -0.36735966163497125 1102 94-95 -0.0768494947121603 1102 96-97 -0.4893862029828959 1102 98-99 -0.27270091898113336 1102 100-101 -0.08605431805250419 1103 1 0.17115594037821324 1103 2 0.2670067563217664 1103 3 0.21137283927745898 1103 4 0.2068321871166745 1103 5 0.3430004427633122 1103 6 0.07289787378867629 1103 7 0.281688469027344 1103 8 0.37424235865310607 1103 9 0.34877230499294853 1103 10-11 0.2601084931647293 1103 12-13 0.39525542294057914 1103 14-15 0.23061646217682608 1103 16-17 0.3360769338263836 1103 18-19 0.35764369123292994 1103 20-21 0.2634580052781459 1103 22-23 0.23134499046756218 1103 24-25 0.17607794724468562 1103 26-27 0.08340257898451142 1103 28-29 0.2554970306884812 1103 30-31 0.5183596700341724 1103 32-33 0.5425181713887923 1103 34-35 0.3383318742303345 1103 36-37 0.3821741319071137 1103 38-39 0.46994596569349767 1103 40-41 0.4692422132696876 1103 42-43 0.29471719864546486 1103 44-45 0.4404229324557676 1103 46-47 0.5107467872663278 1103 48-49 0.546157895107882 1103 50-51 0.5179116028794652 1103 52-53 0.533570777019186 1103 54-55 0.34243472468374847 1103 56-57 0.49420801668455994 1103 58-59 0.4559548368417907 1103 60-61 0.31985456876073215 1103 62-63 0.3584749867481136 1103 64-65 0.1840024598713903 1103 66-67 0.5323088999986894 1103 68-69 -3.7464088111883953 1103 70-71 0.7229282657086102 1103 72-73 0.8885424122548997 1103 74-75 0.6601843039807314 1103 76-77 0.6418886748267525 1103 78-79 0.4503366117930838 1103 80-81 0.5690718220757951 1103 82-83 0.41875433742633916 1103 84-85 0.40351660113687515 1103 86-87 0.49390838561029327 1103 88-89 0.3437011212202954 1103 90-91 0.0996155370805134 1103 92-93 0.3472808949633617 1103 94-95 0.1591797011993492 1103 96-97 -0.38601638711541497 1103 98-99 0.22729586865809637 1103 100-101 0.09342546730249168 1104 1 0.2579685409027377 1104 2 0.3094738903953811 1104 3 0.306982940314235 1104 4 0.2707111366393633 1104 5 0.2135348799164447 1104 6 0.20552007175541576 1104 7 0.1226723024587244 1104 8 0.23769380539937401 1104 9 0.06952868464788509 1104 10-11 0.37207221362231024 1104 12-13 0.17733365761134223 1104 14-15 0.2654208706097094 1104 16-17 0.29777186350261786 1104 18-19 0.3302181313397554 1104 20-21 0.5006599404348435 1104 22-23 0.34728714702395536 1104 24-25 0.3784630039149661 1104 26-27 0.28484357924907044 1104 28-29 0.2524985940908735 1104 30-31 0.3410396654764867 1104 32-33 0.34940349922161573 1104 34-35 0.5078541831561054 1104 36-37 0.4100574161182138 1104 38-39 0.30390964800739084 1104 40-41 0.3188093557055751 1104 42-43 0.27768481709508563 1104 44-45 0.3307933126065663 1104 46-47 0.39580688069881376 1104 48-49 0.36077490064214146 1104 50-51 0.2705499972451548 1104 52-53 0.11722331927187923 1104 54-55 0.0034018433531812775 1104 56-57 0.057251717131372004 1104 58-59 0.07541477104880556 1104 60-61 0.276977389985646 1104 62-63 0.5475349354301784 1104 64-65 0.3190030149110292 1104 66-67 0.18821660008558538 1104 68-69 -3.8646855573748837 1104 70-71 0.8174725439345742 1104 72-73 1.0239640149844256 1104 74-75 0.8121773853016201 1104 76-77 0.751953034973635 1104 78-79 0.5062833847143864 1104 80-81 0.356243951359442 1104 82-83 0.066223478934063 1104 84-85 0.2348356540583154 1104 86-87 0.2489006859635161 1104 88-89 0.4431977222749204 1104 90-91 0.2883708252389958 1104 92-93 0.13577949562774805 1104 94-95 0.6054609111759781 1104 96-97 0.09898629516864688 1104 98-99 0.5214962179174023 1104 100-101 0.6572887681305524 1105 1 0.18399916964995455 1105 2 0.19411393771173024 1105 3 0.13993612269982592 1105 4 0.0911247057346074 1105 5 0.11741427397828375 1105 6 0.10792180590741651 1105 7 0.1783768602685072 1105 8 0.25617529024262353 1105 9 0.3271953874264497 1105 10-11 0.29027290404280137 1105 12-13 0.1850847841562171 1105 14-15 0.21990987258415373 1105 16-17 0.18866195351885295 1105 18-19 0.16868740645680447 1105 20-21 0.35729179327787364 1105 22-23 0.39067413092877956 1105 24-25 0.22606932174067396 1105 26-27 0.17228766680383956 1105 28-29 0.2685215417393607 1105 30-31 0.38609706875759287 1105 32-33 0.23529453107523324 1105 34-35 0.32552080785488613 1105 36-37 0.009428093060179776 1105 38-39 0.30756719788328724 1105 40-41 0.31893037937057755 1105 42-43 0.3391794788778597 1105 44-45 0.2756677651940791 1105 46-47 0.24374796559789047 1105 48-49 0.2191833125676581 1105 50-51 0.26475232929309556 1105 52-53 0.34158387024188386 1105 54-55 0.2690156041342959 1105 56-57 0.19181319666520125 1105 58-59 0.22351943309511313 1105 60-61 0.25092655809434916 1105 62-63 0.17088781798939578 1105 64-65 0.38126627456632534 1105 66-67 0.9019475644782808 1105 68-69 2.0869427965178318 1105 70-71 2.3017946113310934 1105 72-73 1.3512159091511826 1105 74-75 0.5492727490216502 1105 76-77 0.2910009788889276 1105 78-79 0.2675110136363088 1105 80-81 0.3608755465391873 1105 82-83 0.21941674689509583 1105 84-85 0.08888453005102903 1105 86-87 0.06202135924548813 1105 88-89 0.3980798071613094 1105 90-91 0.44072644351864554 1105 92-93 0.11879641063692503 1105 94-95 0.20234303900624795 1105 96-97 0.11687534965174606 1105 98-99 0.4587334916446295 1105 100-101 0.5767650870200995 1106 1 0.37638868650084945 1106 2 0.31080662490338895 1106 3 0.23153773805401556 1106 4 0.25562126061709733 1106 5 0.33196599237375324 1106 6 0.4232926504295804 1106 7 0.46963500469282593 1106 8 0.33370604650316693 1106 9 0.3743091907945484 1106 10-11 0.3825526142782323 1106 12-13 0.4947597357580449 1106 14-15 0.5852546965813232 1106 16-17 0.49049744135165696 1106 18-19 0.4953704140131592 1106 20-21 0.5479738432789176 1106 22-23 0.5414203995854976 1106 24-25 0.481656448866552 1106 26-27 0.5377631138364549 1106 28-29 0.44153669609311663 1106 30-31 0.6127566578414587 1106 32-33 0.7486977034976476 1106 34-35 0.6959854303491113 1106 36-37 0.7733735332233778 1106 38-39 0.9577456986847181 1106 40-41 0.7979219279040635 1106 42-43 0.5412194880370933 1106 44-45 0.6017564639596671 1106 46-47 0.6487428655749881 1106 48-49 0.6500686349099212 1106 50-51 0.7074056942673934 1106 52-53 0.6806203822425445 1106 54-55 0.7609060473158777 1106 56-57 0.6726183757904991 1106 58-59 0.8137721444338126 1106 60-61 0.9664196990839571 1106 62-63 0.6844700116237306 1106 64-65 0.7622999139010354 1106 66-67 1.2851377849241636 1106 68-69 2.6906845480114114 1106 70-71 2.94828027922593 1106 72-73 1.8003296500700188 1106 74-75 1.3682172524459553 1106 76-77 0.7975451409239405 1106 78-79 0.7565912401189578 1106 80-81 0.7954063444325641 1106 82-83 0.8091422823974987 1106 84-85 0.8954562738425302 1106 86-87 0.8343724698014974 1106 88-89 1.0212901154524907 1106 90-91 0.6597084164989333 1106 92-93 0.5986128098126002 1106 94-95 1.03901636692731 1106 96-97 1.0396417477865967 1106 98-99 0.9416687109456134 1106 100-101 1.0269029958025477 1107 1 0.09456151113846545 1107 2 0.015017558706993839 1107 3 0.08311414994270194 1107 4 0.026417854018966125 1107 5 0.0860586415199549 1107 6 -0.024279288148427725 1107 7 0.11703919412033059 1107 8 0.010229767953546798 1107 9 0.08663875335637528 1107 10-11 0.18818296397069645 1107 12-13 -0.12442711098805148 1107 14-15 0.16437960517954053 1107 16-17 0.08473267727577394 1107 18-19 0.1707854856291462 1107 20-21 0.08923153178164256 1107 22-23 0.17633695513627146 1107 24-25 0.22577340013327785 1107 26-27 0.23449953260715972 1107 28-29 0.372303610799122 1107 30-31 0.1558660388901103 1107 32-33 0.16164019564784127 1107 34-35 0.41348940901485776 1107 36-37 0.38391914808853045 1107 38-39 0.36009956336707205 1107 40-41 0.2932163491694979 1107 42-43 0.3645699547238266 1107 44-45 0.4624289660027685 1107 46-47 0.2613907213967508 1107 48-49 0.34920945817113846 1107 50-51 0.3989892032355158 1107 52-53 0.526797637178241 1107 54-55 0.28892123076462184 1107 56-57 0.3133169004582257 1107 58-59 0.4451315577419095 1107 60-61 0.5517803597575366 1107 62-63 0.551438483385958 1107 64-65 0.525457469064694 1107 66-67 0.8941822599553468 1107 68-69 2.103104437038894 1107 70-71 2.579903144725524 1107 72-73 1.6384527951515295 1107 74-75 0.9242400921379819 1107 76-77 0.605730439216341 1107 78-79 0.6118140454009051 1107 80-81 0.7343693105816449 1107 82-83 0.5700642575362167 1107 84-85 0.6086901763759727 1107 86-87 0.5346187169519148 1107 88-89 0.6860165552993642 1107 90-91 0.6060803034618125 1107 92-93 0.4927684724217407 1107 94-95 0.5192811094968839 1107 96-97 0.37891870593533383 1107 98-99 0.7552961787936923 1107 100-101 0.9263535098031497 1108 1 -0.2060874221644582 1108 2 -0.2743216903548529 1108 3 -0.17614507092268283 1108 4 -0.10446141950745869 1108 5 -0.1783153348033082 1108 6 -0.20005106265965367 1108 7 -0.13291519395422569 1108 8 -0.07476598184430827 1108 9 -0.005042253423212628 1108 10-11 -0.11746617665916403 1108 12-13 -0.10216123492809004 1108 14-15 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0.10364849225706863 2316 68-69 1.3302611216648828 2316 70-71 1.4062899204607469 2316 72-73 0.5498383179109396 2316 74-75 0.27618981200839343 2316 76-77 -0.25142584266741963 2316 78-79 -0.19249910010538684 2316 80-81 -0.6181319423941716 2316 82-83 -0.4708509965493768 2316 84-85 -0.5638535435302359 2316 86-87 -0.6115218937842997 2316 88-89 -0.6029867919360363 2316 90-91 -0.6092055540211376 2316 92-93 -0.6529817020998898 2316 94-95 -0.18273551033235336 2316 96-97 -0.3373583449175861 2316 98-99 -0.40315491935011494 2316 100-101 -0.22129738428459333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 27.0 5 109.0 6 230.0 7 466.0 8 1045.0 9 1690.0 10 2772.0 11 4090.0 12 5836.0 13 7441.0 14 9032.0 15 11419.0 16 13905.0 17 16366.0 18 14762.0 19 12093.0 20 11841.0 21 12668.0 22 13279.0 23 14805.0 24 17903.0 25 21494.0 26 26067.0 27 32168.0 28 39052.0 29 47230.0 30 56330.0 31 66323.0 32 78356.0 33 94566.0 34 117419.0 35 148195.0 36 176676.0 37 180689.0 38 93102.0 39 6666.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1652718292002 19.508302276165278 14.874472003557958 22.451953891076556 2 16.4181174080857 21.274508574635952 38.02401679892551 24.28335721835284 3 19.463352513009514 25.685501978802805 29.791840415655514 25.05930509253217 4 13.59780786846031 17.387508360229862 35.74697870325922 33.26770506805061 5 13.805016211656318 37.95112980164779 33.08040642331116 15.163447563384738 6 31.62987428131519 36.07113808998152 16.881654540185515 15.417333088517783 7 28.694226113707977 33.2827479513289 20.11646436200846 17.906561572954665 8 27.196721831279312 34.11018027125858 18.97320523285867 19.719892664603435 9 27.174673683729278 16.637281426537267 19.592765552977127 36.59527933675633 10-11 25.797733686398654 26.56193427882279 26.02415120162404 21.616180833154512 12-13 26.979411159607203 23.9561971886031 26.861317151910264 22.203074499879435 14-15 23.555016764703495 25.235432184248772 25.63580147228533 25.5737495787624 16-17 22.410041902711235 28.656477038309735 26.18312941172133 22.750351647257705 18-19 22.288280635311104 26.705800285077387 29.048360657550976 21.957558422060536 20-21 23.350831746087927 25.587059580878723 28.90372038524529 22.158388287788057 22-23 22.98976567759709 25.632372579773367 28.994899430213927 22.382962312415614 24-25 22.89157825887218 26.098961670824362 28.94760128336631 22.061858786937155 26-27 22.648188452418832 26.102751269338725 29.222277240316945 22.026783037925494 28-29 22.201144956406928 26.108390730476582 29.554873954877937 22.13559035823855 30-31 22.258264684486914 26.142075551768595 29.28609009624173 22.313569667502755 32-33 22.282198708489943 26.24086802690388 29.027193683001162 22.449739581605016 34-35 22.818166365609667 26.08617420157213 28.820133099272116 22.275526333546082 36-37 22.89324494902893 25.888247495737033 28.864389638957988 22.35411791627605 38-39 22.532525982588595 25.97742178466735 29.188113535451503 22.301938697292556 40-41 22.272266582612808 26.030514797883203 29.41162792586037 22.285590693643623 42-43 22.430661977694903 26.189110542214035 28.65901460726909 22.721212872821976 44-45 22.34570129333778 26.2679364207653 28.444204301825316 22.942157984071606 46-47 22.487237314538337 26.4985937334504 28.109166584552952 22.905002367458316 48-49 22.68415542065384 26.391418449845677 27.987173142281208 22.937252987219274 50-51 22.458193831818747 26.58134931179253 27.930786581873363 23.02967027451536 52-53 22.598125420179073 26.731063991197544 27.698573586593234 22.972237002030145 54-55 22.296811705416633 26.44037719421222 27.766459819814504 23.49635128055664 56-57 22.447121931213147 26.811766320022777 27.528978376679802 23.212133372084274 58-59 22.634125439777453 26.25264438030697 27.60602135121611 23.50720882869947 60-61 22.575484040348616 26.359420700274782 27.984811007815736 23.08028425156086 62-63 22.522896541552985 26.37301821399602 27.864759236044538 23.23932600840646 64-65 22.259851967001193 26.446205115006904 27.811421268700464 23.482521649291435 66-67 22.05860196073508 26.56601047872128 27.842504553236306 23.532883007307333 68-69 21.5812703185053 26.42946007390567 28.34315780119523 23.6461118063938 70-71 21.89765295027693 26.55051178707703 27.964242836850445 23.587592425795595 72-73 22.07180218383841 26.37660869693701 27.70250003693908 23.849089082285495 74-75 22.074519482550308 26.85586910629434 27.143685375953545 23.9259260352018 76-77 22.082445565722956 26.673634804891517 27.37014128183009 23.87377834755544 78-79 22.073699075627466 26.533510808927367 27.50570588951038 23.88708422593479 80-81 21.96487057181276 27.140560113767464 27.147049313121936 23.74752000129784 82-83 22.12253027382492 26.846736503057983 27.006788474783246 24.023944748333854 84-85 21.949441659326467 26.49805529244722 26.983190603462166 24.56931244476415 86-87 22.005416976683993 26.509338864853582 27.194977977401486 24.290266181060936 88-89 21.973469152239165 26.412716578443124 27.087693967972587 24.526120301345124 90-91 22.02715108969372 26.5154135158154 27.18342470955634 24.274010684934538 92-93 22.04116245971875 26.376641668325853 27.150152276028876 24.432043595926526 94-95 21.62629981222116 26.158526078160126 27.422094289405237 24.793079820213475 96-97 21.80518392428851 26.091059412162103 27.259300943148528 24.84445572040086 98-99 21.940583777787857 26.097455112951007 26.9903468275664 24.97161428169474 100-101 21.703082451285322 25.99288707634501 27.267534794881204 25.036495677488464 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2375.0 1 1537.5 2 857.0 3 1088.0 4 960.0 5 917.0 6 1633.0 7 3280.5 8 4632.0 9 5005.5 10 4201.5 11 2972.5 12 2630.0 13 2524.0 14 2426.5 15 2398.5 16 2376.0 17 2423.0 18 2518.5 19 2529.5 20 2593.5 21 2846.0 22 3163.0 23 3637.5 24 4243.5 25 4945.0 26 5740.0 27 6553.0 28 7562.5 29 8951.0 30 10725.0 31 12721.5 32 15246.0 33 18139.0 34 20669.5 35 22866.0 36 26350.5 37 29415.5 38 31089.0 39 32141.0 40 32525.5 41 34838.0 42 37668.5 43 39889.5 44 42163.5 45 44438.0 46 46822.5 47 50283.0 48 57799.0 49 58827.0 50 53711.0 51 51170.0 52 49376.0 53 49558.0 54 49439.5 55 47153.0 56 43893.5 57 40619.0 58 37129.5 59 32806.5 60 28275.0 61 23622.5 62 19239.5 63 15477.5 64 12261.0 65 9784.5 66 7630.5 67 6225.5 68 4581.0 69 2927.5 70 2115.5 71 1613.5 72 1272.5 73 1015.5 74 786.0 75 612.5 76 398.0 77 215.0 78 114.0 79 65.5 80 39.0 81 20.5 82 12.5 83 5.5 84 3.5 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021384490934819336 2 0.02374415890004078 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.0556793152243565E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.4747924782634023E-4 26-27 2.580886836960954E-4 28-29 1.1060943586975518E-4 30-31 4.7930755543560576E-4 32-33 0.0026546264608741242 34-35 0.004977424614138983 36-37 0.10566888106757277 38-39 0.09744691300125431 40-41 0.1058900999393123 42-43 0.10729115279366251 44-45 0.10120763382082598 46-47 0.09545594315559872 48-49 0.09656203751429628 50-51 0.09578777146320798 52-53 0.07713164661317595 54-55 0.06289989919793411 56-57 0.03369900812831875 58-59 0.013383741740240377 60-61 0.010397286971756986 62-63 0.0016960113500029128 64-65 0.005456732169574589 66-67 0.1578765347980972 68-69 0.4242240563724677 70-71 0.40648967682135023 72-73 0.1875198636111916 74-75 0.02927463069352854 76-77 0.031597428846793396 78-79 0.005014294426095568 80-81 0.0017328811619594978 82-83 7.373962391317012E-5 84-85 4.7930755543560576E-4 86-87 0.0011798339826107219 88-89 2.580886836960954E-4 90-91 5.899169913053609E-4 92-93 0.0024334075891346138 94-95 0.07901200702296178 96-97 0.01762377011524766 98-99 0.018250556918509603 100-101 0.010360417159800402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1356123.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.28529204971465 #Duplication Level Percentage of deduplicated Percentage of total 1 73.97841669958743 36.46047872414653 2 14.004824646474795 13.804637456131038 3 4.98101384692067 7.364721664474737 4 2.298049364101354 4.530401362175851 5 1.2445890197960048 3.0669966661257093 6 0.7526806366980071 2.2257650999895873 7 0.46285418409123624 1.5968332547558275 8 0.3436441278192846 1.3549280960594328 9 0.26569409949843364 1.178533016069962 >10 1.4542315294642831 13.507512460210403 >50 0.13449750636516225 4.610847967298006 >100 0.07426396022242313 6.184783589039529 >500 0.002844777150215288 0.9685998207352919 >1k 0.001946426471199934 1.4942235083093802 >5k 4.49175339507677E-4 1.650737314478781 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8802 0.6490561696837234 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7974 0.5879997610836185 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5594 0.4124994561702736 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3445 0.25403300438087106 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2275 0.16775764440246202 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1828 0.134796032513275 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1766 0.13022417583065843 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1752 0.12919182109587404 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1549 0.11422267744150051 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1377 0.10153946212843525 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.373962391317012E-5 0.0 12-13 0.0 0.0 0.0 1.1060943586975518E-4 0.0 14-15 0.0 0.0 0.0 1.4747924782634023E-4 0.0 16-17 0.0 0.0 0.0 1.843490597829253E-4 0.0 18-19 0.0 7.373962391317012E-5 0.0 2.580886836960954E-4 0.0 20-21 0.0 7.373962391317012E-5 0.0 5.161773673921908E-4 0.0 22-23 0.0 7.373962391317012E-5 0.0 0.0012904434184804771 0.0 24-25 0.0 7.373962391317012E-5 0.0 0.00306019439239656 0.0 26-27 0.0 7.373962391317012E-5 0.0 0.005641081229357514 0.0 28-29 0.0 7.373962391317012E-5 0.0 0.013383741740240377 0.0 30-31 0.0 7.373962391317012E-5 0.0 0.03439953455549386 0.0 32-33 0.0 7.373962391317012E-5 0.0 0.07414519184469255 0.0 34-35 0.0 7.373962391317012E-5 0.0 0.13660265329914764 0.0 36-37 0.0 7.373962391317012E-5 0.0 0.23567183802649172 0.0 38-39 0.0 7.373962391317012E-5 0.0 0.37902166691369443 0.0 40-41 0.0 7.373962391317012E-5 0.0 0.5455626075215891 0.0 42-43 0.0 7.373962391317012E-5 0.0 0.7076054310707804 0.0 44-45 0.0 7.373962391317012E-5 0.0 0.9008769853471994 0.0 46-47 0.0 7.373962391317012E-5 0.0 1.130686523272594 0.0 48-49 0.0 7.373962391317012E-5 0.0 1.3785253992447588 0.0 50-51 0.0 7.373962391317012E-5 0.0 1.6422920339821683 0.0 52-53 0.0 7.373962391317012E-5 0.0 1.886591408006501 0.0 54-55 0.0 7.373962391317012E-5 0.0 2.1485145521460813 0.0 56-57 0.0 7.373962391317012E-5 0.0 2.447196898806377 0.0 58-59 0.0 7.373962391317012E-5 0.0 2.757603845668866 0.0 60-61 0.0 1.4747924782634023E-4 0.0 3.088178579671608 0.0 62-63 0.0 1.4747924782634023E-4 0.0 3.3932394038003926 0.0 64-65 0.0 1.4747924782634023E-4 0.0 3.7015816411933136 0.0 66-67 0.0 1.4747924782634023E-4 0.0 4.025962246787349 0.0 68-69 0.0 1.4747924782634023E-4 0.0 4.393628011618415 0.0 70-71 0.0 1.4747924782634023E-4 0.0 4.822682013357196 0.0 72-73 0.0 1.4747924782634023E-4 0.0 5.26043729071773 0.0 74-75 0.0 1.4747924782634023E-4 0.0 5.701732070026097 0.0 76-77 0.0 1.4747924782634023E-4 0.0 6.155525715587745 0.0 78-79 0.0 1.4747924782634023E-4 0.0 6.660384050709265 0.0 80-81 0.0 1.4747924782634023E-4 0.0 7.201596020419977 0.0 82-83 0.0 1.4747924782634023E-4 0.0 7.742549901446992 0.0 84-85 0.0 1.4747924782634023E-4 0.0 8.275687382339214 0.0 86-87 0.0 1.4747924782634023E-4 0.0 8.805727799027078 0.0 88-89 0.0 1.4747924782634023E-4 0.0 9.370499578578048 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8970 0.0 26.928999 1 GTACATG 8560 0.0 24.947882 1 TACCTGG 810 0.0 23.432673 2 TACATGG 8970 0.0 23.223032 2 GAGTACT 6530 0.0 22.843472 12-13 GTATCAA 11635 0.0 22.39241 1 ACATGGG 8940 0.0 22.388735 3 CGTATAG 110 0.0028929983 21.571096 1 GTACTTT 6955 0.0 21.311182 14-15 AGTACTT 6895 0.0 20.258425 12-13 ATGGGAG 1900 0.0 20.220232 5 CATGGGG 3510 0.0 19.863916 4 CATGGGA 5485 0.0 19.71573 4 ACTTTTT 7720 0.0 19.690893 16-17 GTACTAC 220 1.3569952E-6 19.413986 1 GTATACC 150 7.7988155E-4 18.972067 3 ATCAACG 13740 0.0 18.916836 3 TATCAAC 13790 0.0 18.822178 2 TCAACGC 13840 0.0 18.814423 4 CAACGCA 14070 0.0 18.506866 5 >>END_MODULE