FastQCFastQC Report
Fri 27 May 2016
SRR939819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158041
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33402.1133756430293404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24551.5533943723464165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20691.3091539537208698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18741.185768250011073No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6930.4384938085686626No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.40242721825349115No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.3575021671591549No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.314475357660354No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3950.24993514341215256No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.22272701387614607No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.19678437873716315No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA2840.17970020437734513No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.16008504122348No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2310.14616460285622085No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2290.1448991084591973No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1950.12338570370979682No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1880.11895647332021436No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1830.11579273732765548No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1600.10123955176188457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCA701.2987584E-954.2108881
GTACCCA601.4982124E-647.4345251
ATATCAA608.325623E-539.528771
CGATAGT250.00161995537.971716-17
TACCCAT756.949873E-637.947622
TAAAGTT500.001622548137.947624
GTCCCAG500.001622548137.947621
CAGGATT550.002590371834.497849
TACCTGG600.00396741131.6230162
CATGGGG3200.031.1289084
ACCCATG804.512576E-429.646583
GGGAATT650.00586841429.1904777
GTCATGG700.00842651927.1054441
GTAATGG700.00842651927.1054441
GAGTAAT651.0098509E-525.54166812-13
AGTACTC3000.025.2984145
ATGGGGC1151.2869078E-424.748455
ATGGGAG1351.3475223E-524.595687
TGGGCTT3100.024.4823366
GTATCAA35800.023.849761
Produced by FastQC (version 0.11.5)