FastQCFastQC Report
Fri 27 May 2016
SRR939819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158041
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT38512.4367094614688596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34762.1994292620269427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29861.889383134756171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25121.5894609626615879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23991.5179605292297569No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT19551.2370207730905272RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT9930.6283179681221962RNA PCR Primer, Index 27 (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.5922513778070247No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.4884808372510931No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG5740.36319689194576094RNA PCR Primer, Index 27 (96% over 30bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.3556039255636196No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.32776304882910134No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA4220.2670193177719706No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.26385558177941165No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.23221822185382274No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT3630.22968723305977565RNA PCR Primer, Index 27 (96% over 28bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3470.21956327788358715No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC3470.21956327788358715No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2960.18729317075948645No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.18666042356097468No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG2680.16957624920115666RNA PCR Primer, Index 27 (100% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2670.16894350200264488No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2580.16324877721603887No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2460.15565581083389754No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT2220.14046987806961483RNA PCR Primer, Index 27 (95% over 22bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2000.12654943970235571No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1970.12465119810682038No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1840.11642548452616727No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT1780.11262900133509658No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATAC500.001679631637.6814733
GTCCCGC550.002668862534.2884641
CTTTACA550.002668862534.2884641
ACAGTTC702.161047E-433.612238
GTATATG600.004087408531.4310931
TATATGG650.006073752428.9857462
ATATGGG650.006073752428.9857463
CGCAGAA2151.8189894E-1228.4531449
ATGGGAT1354.5060733E-727.91225
TTGACAA350.00823275127.24325658-59
GCTACCG350.00877438126.88978212-13
GGTATCA26600.025.3457491
ATGGGGA950.00128331824.790445
AGTACTC2850.024.790445
AAACTGA500.001383551224.48147866-67
CTTGACA601.6719804E-423.82257356-57
GTATCAA34850.023.53951
GAGTAAA2253.0158844E-923.0494671
AGACTTA852.41001E-623.04139164-65
CAGTTCA1050.002310301622.4081529
Produced by FastQC (version 0.11.5)