##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939819_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 158041 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.133920944565016 31.0 31.0 33.0 30.0 34.0 2 31.266753563948594 31.0 31.0 34.0 28.0 34.0 3 31.268322777000904 31.0 31.0 34.0 28.0 34.0 4 35.120664890756196 35.0 35.0 37.0 32.0 37.0 5 34.86575635436374 35.0 35.0 37.0 32.0 37.0 6 34.95440423687524 35.0 35.0 37.0 32.0 37.0 7 34.80166539062648 35.0 35.0 37.0 31.0 37.0 8 34.81017584044647 35.0 35.0 37.0 31.0 37.0 9 36.26284318626179 38.0 35.0 39.0 32.0 39.0 10-11 36.16044887086262 38.0 35.0 39.0 31.0 39.0 12-13 36.04676950917799 38.0 35.0 39.0 30.0 39.0 14-15 36.724698021399504 38.0 35.5 40.0 30.5 41.0 16-17 36.677457115558624 38.0 35.5 40.0 30.5 41.0 18-19 36.798296011794406 38.5 35.5 40.0 31.0 41.0 20-21 36.757749571313774 38.5 35.0 40.0 30.5 41.0 22-23 36.46892578508108 38.0 35.0 40.0 30.0 41.0 24-25 36.116479268038034 38.0 35.0 40.0 30.0 41.0 26-27 35.51049094855132 38.0 34.0 40.0 28.0 41.0 28-29 35.00921279921033 37.0 33.0 40.0 27.0 41.0 30-31 34.276833226820884 36.5 33.0 39.0 25.5 40.5 32-33 33.603590207604356 35.0 33.0 39.0 24.5 40.0 34-35 33.13624629051955 35.0 32.5 39.0 22.0 41.0 36-37 32.59199195145564 35.0 32.0 39.0 20.0 41.0 38-39 31.925108041584146 35.0 31.0 39.0 16.0 40.5 40-41 31.312045608418067 35.0 31.0 38.0 8.5 40.0 42-43 30.502717649217608 35.0 30.0 38.0 2.0 40.0 44-45 29.61143310912991 35.0 27.5 38.0 2.0 40.0 46-47 28.674274397150107 34.5 25.5 38.0 2.0 40.0 48-49 27.870445011104714 34.0 23.5 37.0 2.0 40.0 50-51 27.053913225049197 33.0 21.0 36.5 2.0 40.0 52-53 26.41043779778665 33.0 19.0 36.0 2.0 40.0 54-55 25.711903873045603 33.0 8.5 35.5 2.0 39.0 56-57 24.116153403230804 32.0 2.0 35.0 2.0 39.0 58-59 21.206019324099444 27.5 2.0 35.0 2.0 38.0 60-61 20.597269063091225 26.5 2.0 34.0 2.0 37.0 62-63 21.369226972747576 29.0 2.0 35.0 2.0 37.0 64-65 20.62974481305484 29.0 2.0 35.0 2.0 37.0 66-67 16.625805961744103 16.5 2.0 31.5 2.0 35.5 68-69 11.467938699451409 6.0 2.0 21.0 2.0 32.5 70-71 10.654213147221292 6.0 2.0 17.0 2.0 30.0 72-73 12.001800165779766 6.5 2.0 23.5 2.0 32.0 74-75 12.421760808904018 6.0 2.0 24.5 2.0 33.0 76-77 12.916040774229472 7.0 2.0 26.0 2.0 32.5 78-79 14.80360159705393 7.0 2.0 30.0 2.0 34.0 80-81 16.366022108187117 12.5 2.0 32.5 2.0 35.0 82-83 16.872694427395423 15.0 2.0 33.0 2.0 35.0 84-85 16.828614093811098 11.0 2.0 33.0 2.0 35.0 86-87 16.621788017033555 4.5 2.0 33.0 2.0 35.0 88-89 16.268778354983834 2.0 2.0 33.0 2.0 35.0 90-91 15.230364272562184 2.0 2.0 32.5 2.0 35.0 92-93 13.917366379610355 2.0 2.0 31.5 2.0 34.5 94-95 11.402430381989483 2.0 2.0 27.0 2.0 34.0 96-97 10.566606133851343 2.0 2.0 25.0 2.0 33.5 98-99 9.86934086724331 2.0 2.0 23.0 2.0 33.0 100-101 9.403556672002836 2.0 2.0 20.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2680471765096506 1101 2 0.356108375125892 1101 3 0.5286532692740131 1101 4 0.43664481503215313 1101 5 0.5084835430277437 1101 6 0.541433495069505 1101 7 0.5042202335024726 1101 8 0.42058117085787217 1101 9 0.08769949977293834 1101 10-11 0.4831723038411866 1101 12-13 0.7229966139725335 1101 14-15 0.04595795343462328 1101 16-17 0.49148393748232166 1101 18-19 0.5320039156347107 1101 20-21 0.609129735906329 1101 22-23 0.6617221015690902 1101 24-25 0.49251503996379853 1101 26-27 0.27558664359690255 1101 28-29 -0.6254682412278569 1101 30-31 -0.26554993442010044 1101 32-33 -0.030424525540851732 1101 34-35 0.3752227031713389 1101 36-37 -0.6324607477688851 1101 38-39 -0.6607939988406315 1101 40-41 0.02569846912506435 1101 42-43 -0.4018016333776053 1101 44-45 -0.7103552708916396 1101 46-47 -1.451718829243788 1101 48-49 -1.32929133881888 1101 50-51 -2.2122284651293036 1101 52-53 -3.647936349801416 1101 54-55 -4.04115995647755 1101 56-57 -3.480005857240002 1101 58-59 -7.704809231860764 1101 60-61 -8.008040913216027 1101 62-63 -4.451237127736256 1101 64-65 -2.6474004830509834 1101 66-67 -3.0743233134455714 1101 68-69 -4.150198505552837 1101 70-71 -4.258183913491282 1101 72-73 -4.98311783308012 1101 74-75 -5.291583445594508 1101 76-77 -5.707491777949734 1101 78-79 -7.817601404225245 1101 80-81 -5.388271633253607 1101 82-83 -3.959224810156819 1101 84-85 -3.664930864630705 1101 86-87 -3.66548003283536 1101 88-89 -3.6374034941582583 1101 90-91 -6.816857579247609 1101 92-93 -9.029927464569198 1101 94-95 -7.176691620295948 1101 96-97 -6.874215118153267 1101 98-99 -6.586808512256244 1101 100-101 -6.319140415838657 1102 1 0.618047176509652 1102 2 0.42807807209558746 1102 3 0.608956299577045 1102 4 0.572755926143266 1102 5 0.43967041171461574 1102 6 0.5606254142614233 1102 7 0.32757881936105804 1102 8 0.444318544595248 1102 9 0.5835328331062755 1102 10-11 0.4170106876795785 1102 12-13 0.6823400483159645 1102 14-15 0.805238256464925 1102 16-17 1.1796152506136437 1102 18-19 0.69374633987713 1102 20-21 0.5987762005527983 1102 22-23 0.6817978591448437 1102 24-25 0.15362615107490996 1102 26-27 0.513212906223167 1102 28-29 0.9576125668529514 1102 30-31 0.6684147120445445 1102 32-33 0.4277194138530902 1102 34-35 1.5484550264036514 1102 36-37 1.715834706776569 1102 38-39 1.5464661021694717 1102 40-41 0.47860250952910377 1102 42-43 1.1403700837941102 1102 44-45 0.5205790725427057 1102 46-47 -0.1183223645973186 1102 48-49 0.6296101763326334 1102 50-51 0.9731250702242313 1102 52-53 0.46506870070363604 1102 54-55 0.7619334778658846 1102 56-57 1.0502971730630293 1102 58-59 -6.140289029840563 1102 60-61 -5.842131822306936 1102 62-63 -1.3079290469281766 1102 64-65 0.42627375937326306 1102 66-67 0.19284840372614553 1102 68-69 -1.8878878994922328 1102 70-71 -1.410519772077139 1102 72-73 -3.414304701766989 1102 74-75 -4.327568294079356 1102 76-77 -5.151368040575997 1102 78-79 -5.762803424427265 1102 80-81 -3.9922489059808814 1102 82-83 -2.2964722849042936 1102 84-85 -1.6414460161458564 1102 86-87 -1.9587881136434415 1102 88-89 -2.176355514360278 1102 90-91 -5.005493942883971 1102 92-93 -7.928222919114653 1102 94-95 -6.553143640497968 1102 96-97 -6.638987845425994 1102 98-99 -6.0984878051855365 1102 100-101 -5.906766678464919 1103 1 0.5753642496803835 1103 2 0.3843788851037182 1103 3 0.5215579255933029 1103 4 0.6049374979589786 1103 5 0.7091487314978124 1103 6 0.6940942489497672 1103 7 0.9727346459193242 1103 8 0.4924215256250548 1103 9 0.901114133919279 1103 10-11 0.7632166497391921 1103 12-13 0.9611340862563509 1103 14-15 1.1804923215055823 1103 16-17 1.018368638147514 1103 18-19 1.0296203236169745 1103 20-21 0.8298060108508949 1103 22-23 1.2087287534537836 1103 24-25 1.255929674110135 1103 26-27 1.1663294373884767 1103 28-29 1.232680317530459 1103 30-31 1.730237205269475 1103 32-33 2.04840030816203 1103 34-35 2.2249455413088057 1103 36-37 2.5763529994594947 1103 38-39 2.7127093271017166 1103 40-41 2.2822949214532287 1103 42-43 2.500803688130155 1103 44-45 2.3405316470684525 1103 46-47 2.6073720798471243 1103 48-49 2.486063428365153 1103 50-51 2.7177050160236895 1103 52-53 2.4601567765843946 1103 54-55 1.8829531255623593 1103 56-57 2.1366792868841635 1103 58-59 -4.6309733116833804 1103 60-61 -5.735424505233766 1103 62-63 -0.8788978816165205 1103 64-65 1.503763596771634 1103 66-67 0.34596493489145885 1103 68-69 -2.36222733038654 1103 70-71 -1.8841816961963804 1103 72-73 -3.253904972769698 1103 74-75 -4.2630357737541535 1103 76-77 -4.773234571741309 1103 78-79 -6.929131337706398 1103 80-81 -4.025023296224784 1103 82-83 -1.2021294664761104 1103 84-85 -0.6709175608613052 1103 86-87 -0.2475753765160711 1103 88-89 0.15350559675083275 1103 90-91 -4.118298820932752 1103 92-93 -8.38935096789514 1103 94-95 -7.139441065972223 1103 96-97 -6.015512235669896 1103 98-99 -6.692407181879304 1103 100-101 -6.477566136459499 1104 1 0.1504001176861216 1104 2 0.2197447387622553 1104 3 0.5746425740868482 1104 4 -0.12806106732078604 1104 5 0.3229220457015458 1104 6 0.20506985870586902 1104 7 0.2560918912564816 1104 8 0.4858218125690996 1104 9 0.3876994997729426 1104 10-11 0.13237016480375985 1104 12-13 0.2684511594270802 1104 14-15 -0.0588548807899798 1104 16-17 0.34709891074435717 1104 18-19 0.20526594772027096 1104 20-21 0.49469123323253683 1104 22-23 0.7908664865958244 1104 24-25 0.8395738634932073 1104 26-27 0.5967096382493153 1104 28-29 0.7764034165261577 1104 30-31 0.8194768035478077 1104 32-33 0.5305380413040623 1104 34-35 -0.19991098666823603 1104 36-37 0.6191435303059833 1104 38-39 0.552307605437445 1104 40-41 1.2144685226009955 1104 42-43 1.9650432864084877 1104 44-45 2.0324254777714614 1104 46-47 2.0250191386706504 1104 48-49 1.696644490058123 1104 50-51 1.3375041551915565 1104 52-53 1.186555628808211 1104 54-55 1.280765177212288 1104 56-57 1.7483363887493049 1104 58-59 -4.3639001409516744 1104 60-61 -5.075955351718701 1104 62-63 0.041008861568556654 1104 64-65 1.99110219074581 1104 66-67 -0.21630192307123863 1104 68-69 -1.7186477034138008 1104 70-71 -2.3739593145608 1104 72-73 -4.244615159283328 1104 74-75 -5.170460450942102 1104 76-77 -5.3139088902492 1104 78-79 -7.470809960374978 1104 80-81 -4.990410670686764 1104 82-83 -2.608155291440241 1104 84-85 -2.097818565165465 1104 86-87 -1.7253730809637027 1104 88-89 -2.340612050307991 1104 90-91 -5.074611589942796 1104 92-93 -8.363884683820535 1104 94-95 -7.144606058798622 1104 96-97 -7.122076080720112 1104 98-99 -6.480391399956779 1104 100-101 -6.025557528138122 1105 1 0.1667484752109516 1105 2 -0.06596954695202939 1105 3 -0.14537270475195996 1105 4 0.4470344254217622 1105 5 0.5149770495212493 1105 6 0.6609140145500234 1105 7 0.33668776597000516 1105 8 0.15824350852020785 1105 9 0.5188683309417712 1105 10-11 0.3253800960489812 1105 12-13 0.12624336721928842 1105 14-15 -0.07287321539654101 1105 16-17 0.13758783358621685 1105 18-19 0.4034324870632773 1105 20-21 -0.14346766669107325 1105 22-23 0.053280543127527835 1105 24-25 -0.00293950549075106 1105 26-27 0.4567554747657354 1105 28-29 0.08297331721370682 1105 30-31 0.2792552603850922 1105 32-33 -0.2986063437226747 1105 34-35 0.6018460797947114 1105 36-37 1.1188379535298125 1105 38-39 0.714530676484042 1105 40-41 1.0834906769172719 1105 42-43 1.1553412237652516 1105 44-45 1.703930443394075 1105 46-47 1.7339954564705025 1105 48-49 1.4005787910512488 1105 50-51 1.619589716688882 1105 52-53 1.793622091757026 1105 54-55 1.9757231604055647 1105 56-57 1.1596045323703876 1105 58-59 -4.070393647445179 1105 60-61 -5.580768185943299 1105 62-63 -0.8051332316323574 1105 64-65 0.13311899746849676 1105 66-67 -1.0827648718871288 1105 68-69 -2.747601102955434 1105 70-71 -3.104287809595178 1105 72-73 -3.944156794119081 1105 74-75 -4.353271757282819 1105 76-77 -5.860738531196487 1105 78-79 -7.06240659903044 1105 80-81 -4.954505399487376 1105 82-83 -2.3144196153516248 1105 84-85 -1.968826968526809 1105 86-87 -1.6369086042639331 1105 88-89 -1.979611286366051 1105 90-91 -4.669454981845009 1105 92-93 -6.607200191841926 1105 94-95 -6.652665646269974 1105 96-97 -6.876812520750669 1105 98-99 -6.567327992775724 1105 100-101 -6.174984571682812 1106 1 0.2894308243083934 1106 2 0.2040214683220043 1106 3 0.22688082787892938 1106 4 0.3649467018246071 1106 5 -0.17104761763548737 1106 6 0.1371453304039818 1106 7 0.3628251505979563 1106 8 -0.012180407186725972 1106 9 0.36065547461570446 1106 10-11 -0.0588802976453735 1106 12-13 0.22033795187991245 1106 14-15 0.24804486023850814 1106 16-17 -0.20219816657714063 1106 18-19 -0.1352630940851256 1106 20-21 0.27199422990290145 1106 22-23 0.14445509184925243 1106 24-25 0.3522634676367531 1106 26-27 0.20791940517494822 1106 28-29 0.5925706381317823 1106 30-31 0.8829063263003079 1106 32-33 0.864629791211577 1106 34-35 0.18891624233656756 1106 36-37 0.3783740149526693 1106 38-39 0.12554104975857072 1106 40-41 0.11526393510562372 1106 42-43 0.5897935638779686 1106 44-45 0.5070832654148241 1106 46-47 0.08064252009869932 1106 48-49 0.9257972832508727 1106 50-51 0.7393724496791592 1106 52-53 0.6530928097183129 1106 54-55 0.6024077965241652 1106 56-57 0.9386619529372418 1106 58-59 -4.900378128373058 1106 60-61 -6.0960626399169975 1106 62-63 -1.4296533655864572 1106 64-65 0.18433193547388882 1106 66-67 0.4087813177722701 1106 68-69 -2.3821882139576402 1106 70-71 -3.1967488077165527 1106 72-73 -4.655787930278518 1106 74-75 -4.618055715463004 1106 76-77 -5.4068056716032515 1106 78-79 -7.509396715831878 1106 80-81 -4.986156138685283 1106 82-83 -3.3492762681328045 1106 84-85 -2.4598422425609527 1106 86-87 -2.2788876943562286 1106 88-89 -2.225425220859229 1106 90-91 -5.040871301374537 1106 92-93 -7.2464226046492435 1106 94-95 -6.603392592279939 1106 96-97 -6.530104197627252 1106 98-99 -5.960450593445495 1106 100-101 -6.057448020183997 1107 1 0.09365693260721386 1107 2 0.10998864120128005 1107 3 0.3142408524225715 1107 4 0.037864327227275396 1107 5 0.26402678027830007 1107 6 0.023362541632693024 1107 7 0.05810049957786134 1107 8 0.13266542806407955 1107 9 0.13891901196806344 1107 10-11 0.3028507960806621 1107 12-13 0.11967067162220246 1107 14-15 -0.1731662150797888 1107 16-17 0.10373449180605121 1107 18-19 0.4442544699584303 1107 20-21 0.2078547913387041 1107 22-23 0.23007021686842677 1107 24-25 0.22544186923209253 1107 26-27 0.629744071534823 1107 28-29 0.9948754394816817 1107 30-31 1.559505497952408 1107 32-33 0.7953515276742209 1107 34-35 0.3377504193575831 1107 36-37 0.5885481214107173 1107 38-39 0.4017337173456248 1107 40-41 0.7883924824288329 1107 42-43 1.7477549076423458 1107 44-45 1.8283365251172299 1107 46-47 1.787250128627612 1107 48-49 1.845819525926128 1107 50-51 1.68721721114564 1107 52-53 1.4449128741453698 1107 54-55 1.3494165401965077 1107 56-57 1.6976548966402625 1107 58-59 -4.908412336073624 1107 60-61 -5.6957903588923 1107 62-63 -1.6898734913726194 1107 64-65 -0.5358705495698288 1107 66-67 -1.0259862846207355 1107 68-69 -2.240276110874346 1107 70-71 -2.6402792571719917 1107 72-73 -4.616709850818479 1107 74-75 -4.662426017656593 1107 76-77 -5.559819937594968 1107 78-79 -7.889497191364935 1107 80-81 -5.467096466956491 1107 82-83 -3.119812393305377 1107 84-85 -2.9788443901295967 1107 86-87 -3.0158680594428997 1107 88-89 -2.828201720322337 1107 90-91 -5.487201259957141 1107 92-93 -6.840570480090262 1107 94-95 -6.8315142367039305 1107 96-97 -7.329536625913799 1107 98-99 -6.887529133098816 1107 100-101 -6.300736868166815 1108 1 -0.010565540253352879 1108 2 0.2688776867391347 1108 3 -0.10539822836129886 1108 4 0.3164136011593186 1108 5 0.15141575532412332 1108 6 -0.11631742453112537 1108 7 0.1967583312428829 1108 8 0.3237340873055885 1108 9 0.3640000778076242 1108 10-11 0.7163042008459186 1108 12-13 -0.03819623941684824 1108 14-15 0.7149805493358343 1108 16-17 0.36707510931626786 1108 18-19 0.08620780423166252 1108 20-21 0.46301833285851757 1108 22-23 0.6809548919001429 1108 24-25 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22-23 -0.44567389226758536 2312 24-25 -0.4730216832000522 2312 26-27 -0.5336326681647776 2312 28-29 -0.10618216007736692 2312 30-31 -0.779443103398016 2312 32-33 -0.03255600987572649 2312 34-35 0.4401687740496101 2312 36-37 0.11784741864097015 2312 38-39 -0.1074298488663139 2312 40-41 0.30969949634643257 2312 42-43 -0.23133424765597965 2312 44-45 -0.4908380649337545 2312 46-47 -0.3827409145031595 2312 48-49 -0.5545353038933563 2312 50-51 -0.6097374533162565 2312 52-53 0.22931069693715855 2312 54-55 0.565311538129535 2312 56-57 1.1053819188257386 2312 58-59 3.263783474867534 2312 60-61 2.9396478387100125 2312 62-63 0.8306067346160795 2312 64-65 0.5814644373393634 2312 66-67 -1.0369067751815741 2312 68-69 -0.9579797074018366 2312 70-71 -1.3360729735837307 2312 72-73 3.513543697479717 2312 74-75 3.1593667341692306 2312 76-77 3.368111714602911 2312 78-79 3.6018387601301747 2312 80-81 2.0107525064484957 2312 82-83 1.5296811990881736 2312 84-85 0.9524805375264584 2312 86-87 0.8052184776936571 2312 88-89 1.54474853460394 2312 90-91 2.0827828932742225 2312 92-93 2.996035555461617 2312 94-95 5.437381312421053 2312 96-97 6.50048955570395 2312 98-99 6.663576695756083 2312 100-101 5.453638217956927 2313 1 -0.1205242520617773 2313 2 -0.22882668980917487 2313 3 -0.29628179566105217 2313 4 -0.45478375639641655 2313 5 -0.009178794634593146 2313 6 0.0964984301344387 2313 7 0.17357088285312017 2313 8 -0.2955227252460233 2313 9 -0.13515764308420586 2313 10-11 -0.42552899486010887 2313 12-13 -0.29012026914435296 2313 14-15 -0.1048212673445974 2313 16-17 0.18096445696284746 2313 18-19 -0.018515564884772573 2313 20-21 -0.5089222121456132 2313 22-23 -0.4473688075218192 2313 24-25 -0.6874849600362012 2313 26-27 -1.047660109649847 2313 28-29 -0.8032604490200654 2313 30-31 -0.8360694149395798 2313 32-33 -0.3676972528135778 2313 34-35 -0.9007513228026909 2313 36-37 -1.747915293223432 2313 38-39 -1.4381965962432268 2313 40-41 -1.2575482841216896 2313 42-43 -1.3890743606503335 2313 44-45 -1.4358098163461825 2313 46-47 -1.125485063010018 2313 48-49 -1.4689017284292696 2313 50-51 -0.1998908027916393 2313 52-53 0.10193378006871612 2313 54-55 -0.45856255388014944 2313 56-57 0.562201934967792 2313 58-59 2.3789570019054693 2313 60-61 1.8357253205502069 2313 62-63 0.22941222291309415 2313 64-65 0.07883328318278515 2313 66-67 0.3941182449959868 2313 68-69 -0.21032837568270857 2313 70-71 1.6640238787165114 2313 72-73 5.000258790296504 2313 74-75 5.520884086873027 2313 76-77 5.6686121181541615 2313 78-79 4.114736258112416 2313 80-81 1.7190010940191183 2313 82-83 1.4657102547782443 2313 84-85 1.1283158886160471 2313 86-87 0.3710134736581452 2313 88-89 0.6635055967508308 2313 90-91 1.9080774856874587 2313 92-93 3.49981279517106 2313 94-95 5.137074613470286 2313 96-97 5.468512154574006 2313 98-99 5.979944734497003 2313 100-101 5.354106337408096 2314 1 -0.5654788350510422 2314 2 -0.2513535271336984 2314 3 -0.4869011185347105 2314 4 -0.4408118323666912 2314 5 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2.6067854621718745 2314 68-69 0.4129911949200995 2314 70-71 2.308631640896527 2314 72-73 5.66296729566397 2314 74-75 6.991916291662454 2314 76-77 5.848892877856887 2314 78-79 4.859145836972864 2314 80-81 2.819347914828368 2314 82-83 2.4176012539524834 2314 84-85 2.3862514790371883 2314 86-87 2.5568268510322163 2314 88-89 2.417956463802856 2314 90-91 3.9923384270582236 2314 92-93 5.21176985545182 2314 94-95 7.633292615121814 2314 96-97 7.534061287521983 2314 98-99 7.650448450434242 2314 100-101 7.373660424939063 2315 1 -0.665286156823683 2315 2 -0.6802552612377433 2315 3 -0.4713467307259869 2315 4 -0.1754763970890636 2315 5 -0.3369710024268002 2315 6 -0.5525058988698888 2315 7 -1.135173705891468 2315 8 -0.7097218594451604 2315 9 -0.8274520153785758 2315 10-11 -0.763797393128506 2315 12-13 -0.560336719360798 2315 14-15 -0.9101026526259801 2315 16-17 -0.6524554564570693 2315 18-19 -0.5179960843652864 2315 20-21 -0.4954157186391228 2315 22-23 -0.9170657772187951 2315 24-25 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1.8134454510554239 2315 92-93 2.914011929370197 2315 94-95 5.059672016067687 2315 96-97 5.266693972755824 2315 98-99 5.716221790774059 2315 100-101 5.068738372040132 2316 1 -0.7042419801168549 2316 2 -0.9609781528040102 2316 3 -1.3016862707040815 2316 4 -0.7296202452088139 2316 5 -1.0391615829306318 2316 6 -0.9467373702097959 2316 7 -0.48274581249971504 2316 8 -1.047983998912116 2316 9 -1.1014571267330808 2316 10-11 -0.6007269294556323 2316 12-13 -1.1297416116572592 2316 14-15 -1.0869555186354773 2316 16-17 -1.4959202246206331 2316 18-19 -1.2999785597212608 2316 20-21 -1.0335865839184208 2316 22-23 -1.0895374822206207 2316 24-25 -2.990617490156687 2316 26-27 -2.492978526173083 2316 28-29 -1.72026561253125 2316 30-31 -2.2975324097760748 2316 32-33 -1.8582996624521364 2316 34-35 -1.1239010646271268 2316 36-37 -2.1339393896089724 2316 38-39 -2.3671466823017475 2316 40-41 -2.6525052548617936 2316 42-43 -2.118833396794912 2316 44-45 -0.9624363223702801 2316 46-47 -0.6396158719601033 2316 48-49 -1.5324129160368436 2316 50-51 -2.405054313979246 2316 52-53 -0.902317510809084 2316 54-55 -0.6348620375290288 2316 56-57 0.8384497835047959 2316 58-59 2.9993528710965194 2316 60-61 2.216603117452099 2316 62-63 0.9682590387478633 2316 64-65 0.6580759682086033 2316 66-67 3.537560585787727 2316 68-69 6.10746852621058 2316 70-71 3.387380160988455 2316 72-73 6.145852594083079 2316 74-75 5.933224878611407 2316 76-77 5.273121584591337 2316 78-79 3.1570426264428804 2316 80-81 1.68056735907936 2316 82-83 1.1241267780140447 2316 84-85 1.2205017750187999 2316 86-87 0.6475539211624515 2316 88-89 0.4426622232568569 2316 90-91 0.6014337679594011 2316 92-93 1.2799849122106473 2316 94-95 3.2985548200107324 2316 96-97 4.656945889513764 2316 98-99 5.354867281829188 2316 100-101 3.939786199714467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 12.0 5 33.0 6 138.0 7 299.0 8 604.0 9 967.0 10 1663.0 11 2446.0 12 3309.0 13 4043.0 14 4414.0 15 5411.0 16 6507.0 17 7005.0 18 6563.0 19 6282.0 20 6373.0 21 6163.0 22 5256.0 23 4958.0 24 4544.0 25 3664.0 26 3991.0 27 5173.0 28 6768.0 29 7827.0 30 8407.0 31 8405.0 32 8464.0 33 8374.0 34 7681.0 35 6360.0 36 3923.0 37 1647.0 38 351.0 39 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.65989253368607 20.79011158015987 13.430757643842206 20.119238242311855 2 17.297434112696557 22.6133451450628 36.624798304173126 23.464422438067515 3 21.719955200931416 30.14825454476427 29.130151418320793 19.001638835983524 4 13.149752279471782 20.763599319164015 29.13737574426889 36.949272657095314 5 12.302227962544396 39.20175755158376 29.752385293801087 18.743629192070756 6 28.066134737188452 35.82677912693541 14.043824070968927 22.063262064907207 7 21.806999449509938 36.7714706943135 17.62390772014857 23.797622136027993 8 27.432928372563907 31.740698557327256 18.432675272083017 22.393697798025816 9 25.11247081453547 15.862339519491778 17.92699362823571 41.09819603773705 10-11 24.88974380065932 27.427060066691556 22.217652381344084 25.46554375130504 12-13 28.385988446036155 25.38803221948735 23.93113179491398 22.294847539562518 14-15 25.353547497168456 25.32254288444138 24.446188014502564 24.877721603887597 16-17 20.64622471384008 30.62781177036339 24.883732702273463 23.842230813523074 18-19 20.620924648107895 26.780816066971024 30.34292276881899 22.25533651610209 20-21 21.304914547490842 26.03691447156118 29.864402275358927 22.793768705589056 22-23 20.093520035940042 28.722293582045168 29.648002733467898 21.536183648546896 24-25 21.42450376800957 28.80170335545839 29.70020437734512 20.07358849918692 26-27 21.558714380174703 28.507249723963163 30.27610011357848 19.657935782283655 28-29 20.080489008694332 27.692774972474275 31.25150284116076 20.975233177670628 30-31 19.95962702458132 29.157135037477893 31.394734427453624 19.488503510487163 32-33 17.25649997785357 28.320857509855163 33.44396003518119 20.97868247711008 34-35 16.975347701185793 28.40551006719903 31.829370151482557 22.789772080132625 36-37 18.19254880491641 28.95035806622089 32.643044528058276 20.214048600804425 38-39 19.05666049587823 26.446100536383916 32.587459059192106 21.90977990854575 40-41 18.21461460321077 26.84407229136801 33.483650264716026 21.45766284070519 42-43 19.871518987341773 26.653481012658226 33.38481012658227 20.09018987341772 44-45 17.826582278481013 27.64715189873418 32.797784810126586 21.728481012658225 46-47 19.56657267724285 25.976841259141814 30.962078273885812 23.494507789729518 48-49 19.19864806276128 25.46813042143605 31.16196347364324 24.17125804215943 50-51 18.194044492262904 25.60077212746432 31.972722382203095 24.232460998069683 52-53 18.11320874127423 25.82637697852654 32.977551911599974 23.082862368599258 54-55 17.794625792651285 25.444580859923803 31.183945726328048 25.576847621096867 56-57 19.77403635948876 25.762769860531137 29.119907738906175 25.34328604107393 58-59 17.59443688432378 26.39038055204107 31.673364544060984 24.341818019574163 60-61 19.523356181772023 27.96680629601422 27.533637979182537 24.97619954303123 62-63 18.167868893950896 29.6497722095672 26.679321690711213 25.50303720577069 64-65 17.9692598153815 28.607631827368397 27.180227953801023 26.24288040344908 66-67 19.54088262187786 26.303158637727194 27.96798049312607 26.187978247268877 68-69 21.18613209878149 24.34197693017933 28.749170968958477 25.722720002080706 70-71 20.532217324230185 23.860839529490907 28.655517356248605 26.951425790030303 72-73 20.273457892124956 23.414066679126844 28.37214248425829 27.940332944489914 74-75 20.712865179549624 22.76490677941597 28.23830284882619 28.28392519220822 76-77 18.759916065305287 23.216067864271455 28.65486974768412 29.369146322739137 78-79 18.774445478837485 23.32722229459443 28.02170742634186 29.876624800226224 80-81 19.076122892549694 23.723349394617202 26.889942767834828 30.310584944998276 82-83 19.369657240842567 23.78275257686297 26.236862586290897 30.610727596003567 84-85 19.62876721863314 22.974734404363424 25.80121614011554 31.595282236887893 86-87 19.642241338399575 22.884152379626613 25.748309758003536 31.725296523970275 88-89 19.6455350193937 22.43120456084181 25.702823950746957 32.220436469017535 90-91 19.7055456727644 21.89413469557304 26.049980498663444 32.35033913299912 92-93 19.319125468295372 21.904496269484575 26.0822298149639 32.694148447256154 94-95 19.38692456083008 20.901347791813272 27.694782513955378 32.01694513340127 96-97 18.920070615450175 20.73928352872197 27.926744480364484 32.413901375463375 98-99 19.24895679568532 20.785486519819287 27.415025976266705 32.55053070822869 100-101 18.690499258275707 21.4242377075478 26.55245912575006 33.332803908426435 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1200.0 1 797.0 2 369.0 3 339.5 4 317.0 5 339.5 6 432.0 7 560.5 8 712.0 9 732.5 10 656.5 11 593.5 12 572.5 13 587.0 14 607.5 15 595.5 16 556.5 17 604.5 18 632.0 19 653.5 20 713.0 21 759.5 22 861.0 23 979.0 24 1080.5 25 1217.0 26 1361.0 27 1478.5 28 1632.0 29 1888.5 30 2104.5 31 2257.0 32 2452.0 33 2694.5 34 2822.0 35 2846.5 36 2874.0 37 2806.0 38 2724.0 39 2617.5 40 2482.5 41 2398.0 42 2386.5 43 2472.5 44 2661.5 45 2963.5 46 3467.5 47 4110.5 48 4869.0 49 5559.0 50 6003.0 51 6014.0 52 5418.5 53 4462.0 54 3714.0 55 3353.5 56 3164.5 57 3118.5 58 3112.5 59 3210.5 60 3231.0 61 3205.0 62 3165.0 63 3025.0 64 2896.5 65 2717.5 66 2502.0 67 2268.5 68 2000.5 69 1725.5 70 1475.0 71 1226.5 72 1045.0 73 850.0 74 646.5 75 507.5 76 368.5 77 260.0 78 196.0 79 141.5 80 84.5 81 57.5 82 33.0 83 15.0 84 12.5 85 10.5 86 5.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024044393543447586 2 0.0037964831910706717 3 0.0012654943970235573 4 0.0 5 0.05947823666010718 6 0.0 7 0.0 8 6.327471985117786E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0015818679962794463 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.163735992558893E-4 28-29 0.004429230389582451 30-31 0.00917483437842079 32-33 0.0012654943970235573 34-35 0.0018982415955353358 36-37 0.024993514341215255 38-39 0.024360767142703476 40-41 0.02625900873823881 42-43 0.02594263513898292 44-45 0.02594263513898292 46-47 0.028157250333774145 48-49 0.028789997532285925 50-51 0.022778899146424027 52-53 0.020247910352376917 54-55 0.01708417435981802 56-57 0.14426636126068554 58-59 0.7279756518878012 60-61 0.3043514024841655 62-63 6.327471985117786E-4 64-65 0.12781493409937927 66-67 1.3910947159281453 68-69 2.6872773520795237 70-71 2.1782322308767976 72-73 1.8223119317139225 74-75 1.528400858005201 76-77 1.0940199062268652 78-79 0.4274207325947064 80-81 3.163735992558893E-4 82-83 0.0 84-85 0.0 86-87 9.491207977676679E-4 88-89 0.0 90-91 0.2274726178649844 92-93 0.18223119317139222 94-95 1.2705563746116513 96-97 1.2556868154466245 98-99 0.9820236520902803 100-101 0.40337633905125886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 158041.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.664259482741684 #Duplication Level Percentage of deduplicated Percentage of total 1 91.90199893382865 58.50872707105916 2 4.723999945196041 6.014999166148365 3 1.0530011409474087 2.0111561361869668 4 0.6129999997996001 1.5610476420064936 5 0.3689999957619689 1.1746055739660284 6 0.25699999701880405 0.9817028698361387 7 0.16999999802780202 0.7576046790555293 8 0.14099999836423407 0.7181328386341402 9 0.10899999873547173 0.6245463782802229 >10 0.5549999935613469 6.658008179464395 >50 0.05799999932713174 2.538930638717155 >100 0.036999999570756455 4.741077348677695 >500 0.0059999999303929396 2.7725784683082266 >1k 0.0059999999303929396 10.936883009659478 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 3851 2.4367094614688596 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3476 2.1994292620269427 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2986 1.889383134756171 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2512 1.5894609626615879 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2399 1.5179605292297569 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1955 1.2370207730905272 RNA PCR Primer, Index 27 (95% over 23bp) ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 993 0.6283179681221962 RNA PCR Primer, Index 27 (96% over 25bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 936 0.5922513778070247 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 772 0.4884808372510931 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 574 0.36319689194576094 RNA PCR Primer, Index 27 (96% over 30bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 562 0.3556039255636196 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 518 0.32776304882910134 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 422 0.2670193177719706 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 417 0.26385558177941165 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 367 0.23221822185382274 No Hit CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT 363 0.22968723305977565 RNA PCR Primer, Index 27 (96% over 28bp) GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 347 0.21956327788358715 No Hit TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC 347 0.21956327788358715 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 296 0.18729317075948645 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 295 0.18666042356097468 No Hit CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG 268 0.16957624920115666 RNA PCR Primer, Index 27 (100% over 24bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 267 0.16894350200264488 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 258 0.16324877721603887 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 246 0.15565581083389754 No Hit TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT 222 0.14046987806961483 RNA PCR Primer, Index 27 (95% over 22bp) CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 200 0.12654943970235571 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 197 0.12465119810682038 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 184 0.11642548452616727 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 178 0.11262900133509658 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.327471985117785E-4 0.0 3 0.0 0.0 0.0 0.001265494397023557 0.0 4 0.0 0.0 0.0 0.0018982415955353358 0.0 5 0.0 0.0 0.0 0.0018982415955353358 0.0 6 0.0 0.0 0.0 0.0018982415955353358 6.327471985117785E-4 7 0.0 0.0 0.0 0.002530988794047114 6.327471985117785E-4 8 0.0 0.0 0.0 0.002530988794047114 6.327471985117785E-4 9 0.0 0.0 0.0 0.0037964831910706717 6.327471985117785E-4 10-11 0.0 0.0 3.1637359925588927E-4 0.00442923038958245 6.327471985117785E-4 12-13 0.0 0.0 6.327471985117785E-4 0.00442923038958245 6.327471985117785E-4 14-15 0.0 0.0 6.327471985117785E-4 0.004745603988838339 6.327471985117785E-4 16-17 0.0 0.0 6.327471985117785E-4 0.005694724786606008 6.327471985117785E-4 18-19 0.0 0.0 6.327471985117785E-4 0.0072765927828854545 0.001265494397023557 20-21 0.0 0.0 6.327471985117785E-4 0.010123955176188457 0.001265494397023557 22-23 0.0 0.0 6.327471985117785E-4 0.017084174359818023 0.001265494397023557 24-25 0.0 0.0 6.327471985117785E-4 0.04524142469359217 0.001265494397023557 26-27 0.0 0.0 6.327471985117785E-4 0.10661790294923469 0.001265494397023557 28-29 0.0 0.0 6.327471985117785E-4 0.2749286577533678 0.001265494397023557 30-31 0.0 0.0 6.327471985117785E-4 0.6204086281407989 0.001265494397023557 32-33 0.0 0.0 6.327471985117785E-4 1.1781752836289319 0.001265494397023557 34-35 0.0 0.0 6.327471985117785E-4 1.9694256553679108 0.001265494397023557 36-37 0.0 0.0 6.327471985117785E-4 3.0033345777361573 0.001265494397023557 38-39 0.0 0.0 6.327471985117785E-4 4.23529337323859 0.001265494397023557 40-41 0.0 0.0 6.327471985117785E-4 5.5751355660872814 0.001265494397023557 42-43 0.0 0.0 6.327471985117785E-4 6.551780866990212 0.001265494397023557 44-45 0.0 0.0 6.327471985117785E-4 7.391436399415342 0.001265494397023557 46-47 0.0 0.0 6.327471985117785E-4 8.275700609335551 0.001265494397023557 48-49 0.0 0.0 6.327471985117785E-4 9.112825152966636 0.001265494397023557 50-51 0.0 0.0 6.327471985117785E-4 9.842699046449972 0.001265494397023557 52-53 0.0 0.0 6.327471985117785E-4 10.465955036984074 0.001265494397023557 54-55 0.0 0.0 6.327471985117785E-4 11.009801254104948 0.001265494397023557 56-57 0.0 0.0 6.327471985117785E-4 11.46221550104087 0.001265494397023557 58-59 0.0 0.0 6.327471985117785E-4 11.74536987237489 0.001265494397023557 60-61 0.0 0.0 0.001265494397023557 11.977588094228713 0.001265494397023557 62-63 0.0 0.0 0.001265494397023557 12.151909947418709 0.001265494397023557 64-65 0.0 0.0 0.001265494397023557 12.317689713428795 0.001265494397023557 66-67 0.0 0.0 0.001265494397023557 12.455944976303616 0.001265494397023557 68-69 0.0 0.0 0.001265494397023557 12.603058699957607 0.001265494397023557 70-71 0.0 0.0 0.001265494397023557 12.855524832163805 0.001265494397023557 72-73 0.0 0.0 0.001265494397023557 13.193728209768352 0.001265494397023557 74-75 0.0 0.0 0.001265494397023557 13.554394112920065 0.001265494397023557 76-77 0.0 0.0 0.001265494397023557 14.017565062230688 0.001265494397023557 78-79 0.0 0.0 0.001265494397023557 14.658854347922375 0.001265494397023557 80-81 0.0 0.0 0.001265494397023557 15.358040002277889 0.001265494397023557 82-83 0.0 0.0 0.001265494397023557 16.010402363943534 0.001265494397023557 84-85 0.0 0.0 0.001265494397023557 16.556779569858456 0.001265494397023557 86-87 0.0 0.0 0.001265494397023557 17.017103156775775 0.001265494397023557 88-89 0.0 0.0 0.001265494397023557 17.422694111021823 0.001265494397023557 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAATAC 50 0.0016796316 37.681473 3 GTCCCGC 55 0.0026688625 34.288464 1 CTTTACA 55 0.0026688625 34.288464 1 ACAGTTC 70 2.161047E-4 33.61223 8 GTATATG 60 0.0040874085 31.431093 1 TATATGG 65 0.0060737524 28.985746 2 ATATGGG 65 0.0060737524 28.985746 3 CGCAGAA 215 1.8189894E-12 28.453144 9 ATGGGAT 135 4.5060733E-7 27.9122 5 TTGACAA 35 0.008232751 27.243256 58-59 GCTACCG 35 0.008774381 26.889782 12-13 GGTATCA 2660 0.0 25.345749 1 ATGGGGA 95 0.001283318 24.79044 5 AGTACTC 285 0.0 24.79044 5 AAACTGA 50 0.0013835512 24.481478 66-67 CTTGACA 60 1.6719804E-4 23.822573 56-57 GTATCAA 3485 0.0 23.5395 1 GAGTAAA 225 3.0158844E-9 23.049467 1 AGACTTA 85 2.41001E-6 23.041391 64-65 CAGTTCA 105 0.0023103016 22.408152 9 >>END_MODULE