FastQCFastQC Report
Fri 27 May 2016
SRR939818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166292
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35312.123373343275684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27031.625454020638395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21381.2856902316407284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20721.2460010102710894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.4720611935631299No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.40651384311933225No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.3463786592259399No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.3427705481923364No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA4000.2405407355735694No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.22670964327808915No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3300.19844610684819475No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.19363529213672334No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2850.1713852740961682No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2520.15154066341134872No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.149135256055613No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2100.12628388617612393No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1930.11606090491424724No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1670.1004257571019652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA752.581146E-950.1667181
CCTACCC405.644078E-447.0312963
ACCCATG804.5711204E-947.0312963
TACCCAT1107.584458E-834.2045822
GGTCCAG550.00270152334.2045827
CATTACG300.00412496631.3825388-89
GTCCTGG902.5689844E-531.3541981
GTCAAGG753.2508752E-431.3541981
AGCTCAG600.00413733831.3541987
TCATTAA400.00680312728.32789694-95
TAAAGTT350.00874699726.90741520-21
AGGACTT705.171288E-426.8750275
CCCTTGG700.00878610826.8750271
CGCAGAA2156.366463E-1126.2500279
ACCGGCT909.4465615E-426.1284988
GGTATCA26900.025.526281
GTATCAA36850.025.5258061
ATGGGGC1301.0749807E-525.3245455
CTCCAAC950.001294862824.7533151
CAGAGCA1901.3062163E-824.7533159
Produced by FastQC (version 0.11.5)