FastQCFastQC Report
Fri 27 May 2016
SRR939818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166292
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT43162.5954345368388134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35882.1576503980949173No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31461.8918528852861234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26471.5917783176580953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24691.484737690327857No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT19231.1563995862699348RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT10390.6248045606523465RNA PCR Primer, Index 27 (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.5604599138864167No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.487094989536478No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.36201380703822195No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.3469800110648738No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG4460.2682029201645299RNA PCR Primer, Index 27 (96% over 30bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.23813532821783367No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3910.2351285690231641No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT3900.23452721718423014RNA PCR Primer, Index 27 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.20987179178793927No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3290.1978447550092608No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3170.19062853294205376No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3060.18401366271378056No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC3060.18401366271378056No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.17318932961296996No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG2710.16296634835109325RNA PCR Primer, Index 27 (100% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2680.1611622928342915No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2590.15575012628388618No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2060.12387847882038823No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT2030.12207442330358646RNA PCR Primer, Index 27 (95% over 22bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1980.11906766410891684No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1870.11245279388064368No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAC250.0050365556.934687
TATATGG405.405281E-447.4455642
GGGAAAT405.405281E-447.4455649
GTGCAAA459.641342E-442.1865231
GTATATG459.641342E-442.1865231
GGGTGGT704.3218606E-640.6676258
ATGGGGT1251.3460522E-1037.956455
TGGGGTG851.6366483E-533.4909866
GGTACTG300.003951661331.6589430-31
ATCACTA300.003966244431.63513276-77
AATTCAT300.00396916631.63037712-13
GGTGGTA753.0879152E-431.6303759
ATATGGG804.5074732E-429.6534793
CTTCTGG650.00585403529.2060531
GCCAAGA856.426924E-427.9091552
CTTTACA1056.91062E-527.1199071
CTGTTGG350.00843016327.11175258-59
CGTACCG350.00843016327.11175256-57
CCAAGAC700.00841825427.1117523
TACTAAA350.00843016327.11175210-11
Produced by FastQC (version 0.11.5)