##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939818_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 166292 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.087749260337237 31.0 31.0 33.0 30.0 34.0 2 31.240907560195318 31.0 31.0 34.0 28.0 34.0 3 31.21842301493758 31.0 31.0 34.0 28.0 34.0 4 35.089481153633365 35.0 35.0 37.0 32.0 37.0 5 34.83289635099704 35.0 35.0 37.0 32.0 37.0 6 34.90549154499315 35.0 35.0 37.0 32.0 37.0 7 34.72898876674765 35.0 35.0 37.0 31.0 37.0 8 34.76802251461285 35.0 35.0 37.0 31.0 37.0 9 36.203605705626245 38.0 35.0 39.0 31.0 39.0 10-11 36.10731123565776 38.0 35.0 39.0 31.0 39.0 12-13 35.975527986914585 37.0 35.0 39.0 30.0 39.0 14-15 36.64489271883193 38.0 35.5 40.0 30.5 41.0 16-17 36.561996367834894 38.0 35.5 40.0 30.5 41.0 18-19 36.699008370817594 38.0 35.0 40.0 31.0 41.0 20-21 36.673772640896736 38.0 35.0 40.0 30.5 41.0 22-23 36.38175017439204 38.0 35.0 40.0 30.0 41.0 24-25 36.0262309672143 38.0 34.5 40.0 30.0 41.0 26-27 35.41955115098742 38.0 34.0 40.0 27.5 41.0 28-29 34.92515575012628 37.0 33.0 40.0 27.0 41.0 30-31 34.181845789334425 36.5 33.0 39.0 25.5 40.5 32-33 33.51530440430086 35.0 32.5 39.0 23.5 40.0 34-35 32.9622621653477 35.0 32.0 39.0 22.0 40.0 36-37 32.33878057874101 35.0 31.0 39.0 19.0 40.0 38-39 31.67615098741972 35.0 31.0 38.5 14.0 40.0 40-41 31.16723895316672 35.0 31.0 38.0 5.5 40.0 42-43 30.375294662401075 35.0 29.5 38.0 2.0 40.0 44-45 29.52591525749886 35.0 27.5 38.0 2.0 40.0 46-47 28.624669256488588 34.0 25.5 37.5 2.0 40.0 48-49 27.829390469776058 34.0 23.0 37.0 2.0 40.0 50-51 27.088113078199793 33.0 21.5 36.5 2.0 40.0 52-53 26.501509393115725 33.0 19.5 36.0 2.0 39.5 54-55 25.8506873451519 33.0 14.0 35.5 2.0 39.0 56-57 24.992681548120174 33.0 4.0 35.0 2.0 39.0 58-59 24.204775334952974 32.0 2.0 35.0 2.0 39.0 60-61 23.385253048853823 31.0 2.0 35.0 2.0 38.0 62-63 22.70927946022659 31.0 2.0 35.0 2.0 38.0 64-65 22.010737137084163 30.0 2.0 35.0 2.0 37.0 66-67 20.955791619560774 28.5 2.0 34.5 2.0 36.5 68-69 19.424449161715536 25.0 2.0 34.0 2.0 35.0 70-71 18.837433550621796 23.5 2.0 33.0 2.0 35.0 72-73 19.068851778798738 24.5 2.0 33.0 2.0 35.0 74-75 19.05622940369952 25.0 2.0 33.0 2.0 35.0 76-77 18.648467755514396 24.5 2.0 33.0 2.0 35.0 78-79 18.98960262670483 25.0 2.0 33.5 2.0 35.0 80-81 18.855347821903642 25.0 2.0 34.0 2.0 35.0 82-83 18.506202944218604 24.5 2.0 33.0 2.0 35.0 84-85 18.232596877781255 24.0 2.0 33.0 2.0 35.0 86-87 17.925766122242802 23.5 2.0 33.0 2.0 35.0 88-89 17.55241382628148 21.0 2.0 33.0 2.0 35.0 90-91 17.121479084983044 19.0 2.0 33.0 2.0 35.0 92-93 16.698761816563632 16.5 2.0 33.0 2.0 35.0 94-95 16.099072715464363 4.5 2.0 33.0 2.0 35.0 96-97 15.584207298005918 2.0 2.0 32.0 2.0 35.0 98-99 15.041213648281335 2.0 2.0 32.0 2.0 34.5 100-101 13.979869747191685 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.03022587682402289 1101 2 -0.09424772118926583 1101 3 -0.39601798620988404 1101 4 -0.40244397616989147 1101 5 -0.33881983826363893 1101 6 0.09397942882345234 1101 7 0.12812709014064438 1101 8 -0.16388208212398325 1101 9 -0.1805947368057943 1101 10-11 0.0029362484319861437 1101 12-13 0.07141571970834804 1101 14-15 -0.5494513163134584 1101 16-17 -0.3208448024500967 1101 18-19 -0.181057348097994 1101 20-21 0.1544154409691103 1101 22-23 -0.010799695636805495 1101 24-25 0.1922638144394213 1101 26-27 0.1252620188889324 1101 28-29 0.507026014747666 1101 30-31 -0.273532449110661 1101 32-33 -0.7612533892094646 1101 34-35 -0.4315265350529387 1101 36-37 -0.331501918852517 1101 38-39 -0.3832903357806394 1101 40-41 -0.8982574238588263 1101 42-43 -0.6982939449782144 1101 44-45 -1.2914719138891648 1101 46-47 -2.05803441408068 1101 48-49 -1.1838935278943659 1101 50-51 -1.955922950947226 1101 52-53 -2.619047858088006 1101 54-55 -2.4706464424835985 1101 56-57 -2.153356488952305 1101 58-59 -2.6319624727407174 1101 60-61 -2.502297110595247 1101 62-63 -2.1570967104344625 1101 64-65 -2.194123043989233 1101 66-67 -2.2461387185031647 1101 68-69 -6.916224278507915 1101 70-71 -5.626674025829448 1101 72-73 -2.5409192901503097 1101 74-75 -1.8760922945792018 1101 76-77 -1.9113510406443943 1101 78-79 -1.991623376396852 1101 80-81 -2.563240576932504 1101 82-83 -2.6034153811883343 1101 84-85 -2.675161251447175 1101 86-87 -2.8336095783885114 1101 88-89 -2.7554061939811945 1101 90-91 -2.9503701055883234 1101 92-93 -2.946308918462144 1101 94-95 -2.240636530698527 1101 96-97 -2.265923487344166 1101 98-99 -2.3917718356496565 1101 100-101 -2.622130406319677 1102 1 0.39228179188763335 1102 2 0.3695943033506097 1102 3 0.3685142223790727 1102 4 0.27247933671354474 1102 5 0.5298534746197987 1102 6 0.5907969134860309 1102 7 0.7447298508768441 1102 8 0.39585718167970185 1102 9 0.6524957539917509 1102 10-11 0.375731493830763 1102 12-13 0.10145789762245983 1102 14-15 0.365656045649736 1102 16-17 0.13768663926769875 1102 18-19 -0.19060489411026538 1102 20-21 -0.09071492712904217 1102 22-23 0.3781765313570631 1102 24-25 0.7232454095314367 1102 26-27 0.3644107918950681 1102 28-29 0.33714487977835006 1102 30-31 0.4730741459813572 1102 32-33 0.2776653224469783 1102 34-35 0.11726564286117025 1102 36-37 0.45684163942969036 1102 38-39 0.8435118114586224 1102 40-41 0.24271266816571213 1102 42-43 0.1348732329358917 1102 44-45 -0.011544766649898008 1102 46-47 0.5503244816248412 1102 48-49 0.8983725764001136 1102 50-51 0.9887089508932654 1102 52-53 0.5379690130776389 1102 54-55 0.3001549378844999 1102 56-57 0.7353896766918666 1102 58-59 0.4302116070138844 1102 60-61 -0.16610079157684154 1102 62-63 -0.05377999264305444 1102 64-65 -0.19293439368248144 1102 66-67 0.46940959438027363 1102 68-69 -1.8462089410846048 1102 70-71 1.151029195029448 1102 72-73 0.6384902190521409 1102 74-75 0.0031830121692628666 1102 76-77 -0.4322291081290537 1102 78-79 -0.4065006770103494 1102 80-81 0.13587752122700536 1102 82-83 0.5258599255601304 1102 84-85 0.38317847247920866 1102 86-87 0.3540246240654774 1102 88-89 0.8448046955893567 1102 90-91 0.5729044342889758 1102 92-93 0.4258536582249697 1102 94-95 -0.28505754296846497 1102 96-97 -0.4414986407183985 1102 98-99 -0.3317449951588589 1102 100-101 -0.565957093436241 1103 1 0.28359988075173703 1103 2 0.13813864244709606 1103 3 0.36780777136587517 1103 4 0.2926317814058663 1103 5 0.38636955567575626 1103 6 0.2453051863992073 1103 7 0.1574831507467067 1103 8 0.4728603421184445 1103 9 0.6173219298608714 1103 10-11 0.6035991272198657 1103 12-13 0.6673437500113835 1103 14-15 0.08350322914109398 1103 16-17 0.7078483793680874 1103 18-19 0.5698896215989748 1103 20-21 0.24778665309032277 1103 22-23 0.5023631831510684 1103 24-25 0.06953654171214652 1103 26-27 -0.15977585989894294 1103 28-29 -0.029242924646268875 1103 30-31 0.1708804296772115 1103 32-33 0.21999661079053823 1103 34-35 -0.32593941384081404 1103 36-37 0.8109223235717238 1103 38-39 0.74871723997693 1103 40-41 1.4126327276563302 1103 42-43 0.6795469641126921 1103 44-45 0.9118424800502289 1103 46-47 0.7627989192526492 1103 48-49 0.6313526842268473 1103 50-51 0.4539255339012591 1103 52-53 0.9849294146392644 1103 54-55 0.9338043150921607 1103 56-57 0.7745791171082992 1103 58-59 0.7764655575623145 1103 60-61 0.2067937984956636 1103 62-63 -0.4647671649799179 1103 64-65 -0.1840851652013491 1103 66-67 -0.010437960927404788 1103 68-69 -2.8696333694170058 1103 70-71 -0.26237478340520326 1103 72-73 0.06154283106181069 1103 74-75 -0.12940668851859627 1103 76-77 0.12397092905257878 1103 78-79 -0.18480519457867217 1103 80-81 -0.6778239102658361 1103 82-83 -0.8370328054307556 1103 84-85 -0.5144604938714181 1103 86-87 -0.7388179117218456 1103 88-89 -0.831826648526647 1103 90-91 -0.49184737831559744 1103 92-93 -1.116384676037903 1103 94-95 -1.054178197365193 1103 96-97 -1.3903553055259845 1103 98-99 -1.042434714437535 1103 100-101 -1.148834951774223 1104 1 0.5688812660331202 1104 2 0.1614070407154955 1104 3 0.25070820426630647 1104 4 0.3160084047824867 1104 5 0.12695397126017127 1104 6 0.11749133358535602 1104 7 0.11235328061683703 1104 8 0.010522679780777366 1104 9 0.6316076441465839 1104 10-11 0.45457315319389124 1104 12-13 -0.046292613624984824 1104 14-15 0.025548683686544393 1104 16-17 0.3397206737403877 1104 18-19 0.562097413806768 1104 20-21 0.3624511552548313 1104 22-23 -0.13237707658918652 1104 24-25 0.46488286205846663 1104 26-27 0.5118691617460769 1104 28-29 0.35122244331910224 1104 30-31 0.17602112231790557 1104 32-33 -0.1734557701618442 1104 34-35 -0.2477467731481795 1104 36-37 -0.4326923950429915 1104 38-39 -0.2568022405425445 1104 40-41 -0.5811443286207307 1104 42-43 -0.5009725164067866 1104 44-45 0.2542126099203621 1104 46-47 -0.057439175985440016 1104 48-49 -0.41945900408483894 1104 50-51 -0.3481848557091318 1104 52-53 -0.10565500094515201 1104 54-55 -0.5844857281978832 1104 56-57 -0.8588624413332582 1104 58-59 -0.41752794893119116 1104 60-61 -1.110630443928578 1104 62-63 -0.4364121866249384 1104 64-65 -0.504539710655898 1104 66-67 -0.0882518137412589 1104 68-69 -3.618009992793631 1104 70-71 -0.9667037877342075 1104 72-73 -0.12038357586459725 1104 74-75 -0.5634434850553909 1104 76-77 -0.7659641358824878 1104 78-79 -1.0392424240158995 1104 80-81 -0.6611572435991704 1104 82-83 -1.2223141907121438 1104 84-85 -1.4398933943043168 1104 86-87 -1.8114369593408934 1104 88-89 -1.905852622552624 1104 90-91 -1.4179296293978485 1104 92-93 -1.636189870843097 1104 94-95 -1.0291781973651943 1104 96-97 -1.332292534963214 1104 98-99 -1.0112658832687043 1104 100-101 -0.4569518348911039 1105 1 0.4347960530005395 1105 2 0.4236250858282773 1105 3 0.44765891437491234 1105 4 -0.14096736798276055 1105 5 0.16903834887086333 1105 6 0.29240779139654904 1105 7 0.21500574511140513 1105 8 0.5059278593964862 1105 9 0.7915909357088253 1105 10-11 0.009606570069415454 1105 12-13 0.03411674310016366 1105 14-15 0.34974459011929326 1105 16-17 0.4581939402399655 1105 18-19 0.15106984488446074 1105 20-21 0.1397590082205724 1105 22-23 -0.03139545587072945 1105 24-25 0.018872001573917885 1105 26-27 -0.05642657760229497 1105 28-29 0.42594048843188403 1105 30-31 0.7950165274975234 1105 32-33 0.5083007043577865 1105 34-35 0.6902753655318605 1105 36-37 0.6889659174047935 1105 38-39 1.5185736993070797 1105 40-41 1.284509389006672 1105 42-43 0.9242572246124254 1105 44-45 0.8594235539470922 1105 46-47 0.5663076911824767 1105 48-49 0.1777110042693799 1105 50-51 0.08545131805862027 1105 52-53 0.9414419079938661 1105 54-55 0.2575151072240054 1105 56-57 0.16150096718804363 1105 58-59 0.39236135766864066 1105 60-61 0.2146619537322394 1105 62-63 -0.683905920960779 1105 64-65 -0.2757385410652553 1105 66-67 0.47701552126292057 1105 68-69 1.94934589693068 1105 70-71 1.6490021437611944 1105 72-73 0.7830207683292194 1105 74-75 0.020673056297990655 1105 76-77 -0.5925096663753884 1105 78-79 -0.31399179744948214 1105 80-81 -0.15092332547051157 1105 82-83 -0.8601588022409636 1105 84-85 -0.5199894678214427 1105 86-87 -0.47215124505517636 1105 88-89 -0.772602831408097 1105 90-91 -1.1273072400912483 1105 92-93 -0.5295691523802724 1105 94-95 -0.2068097763125607 1105 96-97 -0.018957113075162013 1105 98-99 0.030505211133968757 1105 100-101 -0.058351166553595846 1106 1 0.3903098374616931 1106 2 0.21497846928692255 1106 3 0.17332725188535392 1106 4 0.3576750714491581 1106 5 0.32338254268874067 1106 6 0.5520151431091662 1106 7 -0.19717052890697317 1106 8 0.42123696549506917 1106 9 0.19708383462277368 1106 10-11 0.16885886747960654 1106 12-13 0.060850243517869274 1106 14-15 0.1773343979722526 1106 16-17 -0.17813646911676528 1106 18-19 0.29721646142581903 1106 20-21 0.10054639335006499 1106 22-23 0.07298006626795228 1106 24-25 0.4351209572965544 1106 26-27 0.3273453522222738 1106 28-29 -0.36008708049042326 1106 30-31 0.545068741365526 1106 32-33 0.2491632774572068 1106 34-35 0.5558246554232511 1106 36-37 0.6119504620998626 1106 38-39 0.3414120451717402 1106 40-41 0.517069957093554 1106 42-43 0.5020036740694032 1106 44-45 0.099450705158457 1106 46-47 0.9068465383002717 1106 48-49 0.9466124244865881 1106 50-51 1.4315770490527733 1106 52-53 1.3377973800072347 1106 54-55 1.600038081325927 1106 56-57 0.6589947015238842 1106 58-59 0.7015196701164257 1106 60-61 0.6899647941666593 1106 62-63 0.8969211467083937 1106 64-65 0.879388860772675 1106 66-67 0.8611529481635003 1106 68-69 2.30163286434923 1106 70-71 2.8934152598848364 1106 72-73 1.7784259479449283 1106 74-75 1.7371517530398464 1106 76-77 1.02570253078418 1106 78-79 1.3030194807460056 1106 80-81 1.4162237087817822 1106 82-83 1.7300667616688088 1106 84-85 1.9380827961718765 1106 86-87 1.4683249454210134 1106 88-89 1.7429569012568997 1106 90-91 1.2517132277450074 1106 92-93 1.4769053672521402 1106 94-95 1.4053456121586159 1106 96-97 0.7718741317034539 1106 98-99 1.0512341167312957 1106 100-101 1.2990005460612757 1107 1 0.04961962879395543 1107 2 -0.04010446276230084 1107 3 -0.021228660367185626 1107 4 0.2552807429312338 1107 5 -0.015435006802967166 1107 6 -0.1931694475810417 1107 7 -0.3772802132301365 1107 8 -0.33565174504533246 1107 9 -0.7044009165810792 1107 10-11 -0.05552583021970037 1107 12-13 -0.2916109656849031 1107 14-15 0.05714980728205177 1107 16-17 0.4822099728308018 1107 18-19 0.08670950583459103 1107 20-21 0.23173285669945898 1107 22-23 -0.030919077659284255 1107 24-25 -0.6684103428639574 1107 26-27 -0.11975202605488278 1107 28-29 -0.19321977176917926 1107 30-31 -0.11240183113313407 1107 32-33 0.1969629029253639 1107 34-35 -0.05108411932260282 1107 36-37 0.29771156429355017 1107 38-39 0.3179034844440771 1107 40-41 -0.1349498395891615 1107 42-43 -0.657072034865859 1107 44-45 -0.042771071192536425 1107 46-47 0.2030610915372968 1107 48-49 0.44788315749889307 1107 50-51 0.5319983973673779 1107 52-53 1.0217527037097476 1107 54-55 1.107127433920894 1107 56-57 1.2887432301488175 1107 58-59 1.369758032877261 1107 60-61 1.6875905298541944 1107 62-63 2.2701153682172226 1107 64-65 1.8713966189321205 1107 66-67 2.6376042590249256 1107 68-69 3.0892532495819722 1107 70-71 3.0367740078784173 1107 72-73 2.607746440186766 1107 74-75 2.634757424521922 1107 76-77 2.5778694649735847 1107 78-79 2.776635050569439 1107 80-81 3.070116164640531 1107 82-83 2.303501753643129 1107 84-85 2.2577390294517024 1107 86-87 2.025976095768794 1107 88-89 2.351370491412066 1107 90-91 2.9893068607038096 1107 92-93 3.1209663624367323 1107 94-95 2.6079940872790033 1107 96-97 2.3705231418693185 1107 98-99 2.6234318160357386 1107 100-101 2.1190915037926814 1108 1 -0.24443042227856893 1108 2 -0.21981590300745069 1108 3 -0.1954498043916999 1108 4 -0.23539852162443964 1108 5 -0.40416074735454544 1108 6 -0.11113420754018222 1108 7 -0.10085018258662615 1108 8 0.10657246333056491 1108 9 -0.266390191351249 1108 10-11 0.15208397570471277 1108 12-13 0.3864725378901639 1108 14-15 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-1.9350959467836297 2316 58-59 -1.7719097618973443 2316 60-61 -2.2215742190289802 2316 62-63 -2.0264867706754295 2316 64-65 -1.8152826825434438 2316 66-67 -0.8288570919971399 2316 68-69 0.0797094564318428 2316 70-71 0.5301407332066965 2316 72-73 0.10057167370510811 2316 74-75 0.308508609035254 2316 76-77 -5.453177528274011E-4 2316 78-79 -0.1075872318185418 2316 80-81 -0.03967129982406803 2316 82-83 -0.0633927908268852 2316 84-85 -0.6358916731339193 2316 86-87 -0.2994604820029707 2316 88-89 0.007658565054949662 2316 90-91 0.07124134019480621 2316 92-93 0.23938385262219342 2316 94-95 0.8648981078556908 2316 96-97 0.8591518138606542 2316 98-99 0.7923772607358845 2316 100-101 0.3797521238008059 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 7.0 5 51.0 6 128.0 7 323.0 8 668.0 9 1047.0 10 1868.0 11 2564.0 12 3413.0 13 4016.0 14 4504.0 15 5209.0 16 5866.0 17 6356.0 18 5695.0 19 4858.0 20 4890.0 21 4761.0 22 4538.0 23 4255.0 24 4148.0 25 3983.0 26 3750.0 27 4113.0 28 4764.0 29 5318.0 30 5911.0 31 6911.0 32 8051.0 33 9115.0 34 11249.0 35 13584.0 36 11171.0 37 6739.0 38 2315.0 39 151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.78077725848266 20.880475906911837 13.293754624032337 20.04499221057317 2 17.44305639927584 22.758193442839872 36.176854463764805 23.621895694119488 3 21.90544343684603 29.895003968922136 29.339956221586128 18.859596372645708 4 13.15998364322998 20.830827700671108 29.176388521396095 36.832800134702815 5 12.293435643326198 39.24722776802251 30.068193298535107 18.391143290116183 6 28.13905659923508 35.61806942005629 14.170856084477906 22.07201789623073 7 21.743679792172806 36.791306857816366 17.655689991099994 23.80932335891083 8 27.565968296731054 31.826546075577898 18.626271859139347 21.981213768551704 9 24.984364852187717 16.019411637360786 17.917879392875182 41.07834411757631 10-11 24.937459408750872 27.261383590311016 22.414788444423063 25.386368556515045 12-13 28.322769586029395 25.424554398287352 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20.119434971450588 38-39 18.978739285370317 26.72246701338727 32.50505634209766 21.793737359144753 40-41 18.274391290082747 26.898521758237788 33.32440348083644 21.502683470843028 42-43 19.862314761579377 26.6800518953544 33.21844512608102 20.239188216985195 44-45 17.842577180884845 27.73214463082299 32.76556936422274 21.65970882406942 46-47 19.56455656581161 26.172955435368188 30.929786702542295 23.332701296277904 48-49 19.1809929692767 25.61488731580468 30.952939901762495 24.25117981315612 50-51 18.15215886449687 25.64151954204263 31.99351106670118 24.212810526759327 52-53 18.054619421308555 25.760074141832362 33.111971017283715 23.07333541957537 54-55 17.73528252556585 25.264082653131837 31.22147441008253 25.779160411219788 56-57 19.68502516008915 25.907823539496675 28.909889885132838 25.49726141528133 58-59 17.87204994346477 26.72191882984098 30.657432578728315 24.748598647965935 60-61 19.435039121465987 28.23571510190826 27.195282570650182 25.133963205975572 62-63 18.285770985113402 29.51792194984079 26.567362537246563 25.62894452779924 64-65 18.1248515354616 28.94900637758554 26.740756109221792 26.18538597773107 66-67 18.567686378417438 27.840238468023603 26.73973262676141 26.852342526797546 68-69 19.150284777746588 26.548469564849945 27.06792107717522 27.233324580228246 70-71 19.415653013793726 25.826869095409133 26.533156258489026 28.224321632308108 72-73 19.52076042237656 24.74655599260299 26.45515712624915 29.2775264587713 74-75 19.380656338256923 24.022726110664973 26.696262342810567 29.90035520826754 76-77 18.896645482243883 23.631108317267056 27.19466308947687 30.27758311101219 78-79 19.22005989824515 23.171179081319686 27.439831130248614 30.16892989018655 80-81 19.19273315513006 23.776907140859766 26.91811569581964 30.112244008190537 82-83 19.357515695282995 23.789178072306544 26.22435234406947 30.628953888340995 84-85 19.652537862729 23.067625224380077 25.832047939912744 31.447788972978174 86-87 19.538581346611224 22.75011049873265 25.842270951912756 31.869037202743378 88-89 19.63624227262887 22.26084237365598 25.82866283405095 32.2742525196642 90-91 19.664264258846664 22.108766834143655 25.725307667559676 32.50166123945 92-93 19.44656591837103 21.84870917499053 25.72539374221987 32.979331164418575 94-95 19.498691060090874 21.205729245027534 26.1201215659134 33.175458128968195 96-97 19.270188016504072 21.213807123696334 26.039022747230273 33.47698211256932 98-99 19.489412635067467 21.261341457179892 25.89024988045796 33.35899602729468 100-101 19.34716583972335 21.448804691023906 25.655390166892193 33.54863930236055 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1879.0 1 1132.0 2 445.5 3 524.5 4 414.5 5 327.0 6 534.0 7 894.0 8 1148.5 9 1137.5 10 837.5 11 545.0 12 474.5 13 462.5 14 465.5 15 456.5 16 465.5 17 518.5 18 587.5 19 646.0 20 694.5 21 777.5 22 868.5 23 956.5 24 1090.0 25 1239.0 26 1382.5 27 1574.5 28 1795.0 29 1997.5 30 2258.0 31 2472.5 32 2584.0 33 2729.5 34 2797.5 35 2848.5 36 2896.5 37 2860.5 38 2755.0 39 2641.0 40 2531.5 41 2427.5 42 2384.0 43 2456.5 44 2620.5 45 2775.0 46 3069.5 47 3552.0 48 4599.5 49 5283.0 50 5808.0 51 6799.0 52 6632.5 53 5115.0 54 3611.0 55 3059.0 56 3086.5 57 3097.5 58 3118.0 59 3203.5 60 3262.5 61 3273.0 62 3171.5 63 3147.0 64 3092.0 65 2874.5 66 2744.0 67 2624.0 68 2404.0 69 2230.5 70 1983.5 71 1690.5 72 1445.5 73 1176.0 74 942.0 75 760.5 76 607.5 77 452.5 78 322.5 79 236.0 80 164.0 81 98.5 82 60.5 83 43.0 84 29.5 85 18.0 86 12.0 87 6.5 88 6.5 89 5.5 90 2.5 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02465542539629086 2 0.018641907006951626 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.002104731436268732 34-35 0.004209462872537464 36-37 0.1061385995718375 38-39 0.09862170158516344 40-41 0.10974671060544103 42-43 0.11335482163904458 44-45 0.10373319221610179 46-47 0.09771967382676257 48-49 0.09862170158516344 50-51 0.09802034974622953 52-53 0.0745676280278065 54-55 0.06223991532966108 56-57 0.03397637889976668 58-59 0.014432444134414163 60-61 0.009020277584008852 62-63 9.020277584008852E-4 64-65 0.003908786953070503 66-67 0.13951362663267025 68-69 0.3827604454814423 70-71 0.38306112140090925 72-73 0.16777716306256465 74-75 0.030969619705097057 76-77 0.032172323382964906 78-79 0.006013518389339235 80-81 0.0015033795973348088 82-83 0.0 84-85 3.0067591946696175E-4 86-87 3.0067591946696175E-4 88-89 0.0 90-91 3.0067591946696175E-4 92-93 0.0018040555168017704 94-95 0.07637168354460827 96-97 0.018040555168017704 98-99 0.019543934765352513 100-101 0.0102229812618767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 166292.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.48393447335631 #Duplication Level Percentage of deduplicated Percentage of total 1 91.45812109636061 57.146632456412824 2 4.875279475908675 6.09253286623953 3 1.2350645060810401 2.3151506900500762 4 0.6338859425882123 1.584307508010542 5 0.3889243500033325 1.2150761800350458 6 0.2629486129976911 0.9858038342644595 7 0.18496384262452628 0.8090088035743761 8 0.15696931452617016 0.7846448290544696 9 0.10497947784406184 0.5903577733189967 >10 0.5858854668161625 6.896253396370534 >50 0.0599882730528405 2.635681953010688 >100 0.041991791136988345 5.1701597163110815 >500 0.003999218203522699 1.824799000887466 >1k 0.006998631856164724 11.94959099245993 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 4316 2.5954345368388134 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3588 2.1576503980949173 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3146 1.8918528852861234 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2647 1.5917783176580953 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2469 1.484737690327857 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1923 1.1563995862699348 RNA PCR Primer, Index 27 (95% over 23bp) ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 1039 0.6248045606523465 RNA PCR Primer, Index 27 (96% over 25bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 932 0.5604599138864167 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 810 0.487094989536478 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 602 0.36201380703822195 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 577 0.3469800110648738 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 446 0.2682029201645299 RNA PCR Primer, Index 27 (96% over 30bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 396 0.23813532821783367 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 391 0.2351285690231641 No Hit CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT 390 0.23452721718423014 RNA PCR Primer, Index 27 (96% over 28bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 349 0.20987179178793927 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 329 0.1978447550092608 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 317 0.19062853294205376 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 306 0.18401366271378056 No Hit TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC 306 0.18401366271378056 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 288 0.17318932961296996 No Hit CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG 271 0.16296634835109325 RNA PCR Primer, Index 27 (100% over 24bp) GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 268 0.1611622928342915 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 259 0.15575012628388618 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 206 0.12387847882038823 No Hit TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT 203 0.12207442330358646 RNA PCR Primer, Index 27 (95% over 22bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 198 0.11906766410891684 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 187 0.11245279388064368 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 6.013518389339235E-4 0.0 0.0 3 0.0 0.0 6.013518389339235E-4 0.0 0.0 4 0.0 0.0 6.013518389339235E-4 0.0 0.0 5 0.0 0.0 6.013518389339235E-4 0.0 0.0 6 0.0 0.0 6.013518389339235E-4 0.0 0.0 7 0.0 0.0 6.013518389339235E-4 6.013518389339235E-4 0.0 8 0.0 0.0 6.013518389339235E-4 0.001202703677867847 0.0 9 0.0 0.0 6.013518389339235E-4 0.0018040555168017704 0.0 10-11 0.0 0.0 6.013518389339235E-4 0.0018040555168017704 0.0 12-13 0.0 0.0 6.013518389339235E-4 0.0018040555168017704 0.0 14-15 0.0 0.0 6.013518389339235E-4 0.0021047314362687324 0.0 16-17 0.0 0.0 6.013518389339235E-4 0.002405407355735694 3.0067591946696175E-4 18-19 0.0 0.0 6.013518389339235E-4 0.002405407355735694 6.013518389339235E-4 20-21 0.0 0.0 6.013518389339235E-4 0.0027060832752026556 6.013518389339235E-4 22-23 0.0 0.0 6.013518389339235E-4 0.007817573906141006 6.013518389339235E-4 24-25 0.0 0.0 6.013518389339235E-4 0.030668943785630096 6.013518389339235E-4 26-27 0.0 0.0 6.013518389339235E-4 0.08298655377288144 6.013518389339235E-4 28-29 0.0 0.0 6.013518389339235E-4 0.2691049479229307 6.013518389339235E-4 30-31 0.0 0.0 6.013518389339235E-4 0.6786255502369327 6.013518389339235E-4 32-33 0.0 0.0 6.013518389339235E-4 1.2429942510764198 6.013518389339235E-4 34-35 0.0 0.0 6.013518389339235E-4 2.03467394703293 6.013518389339235E-4 36-37 0.0 0.0 6.013518389339235E-4 3.0548673417843313 6.013518389339235E-4 38-39 0.0 0.0 6.013518389339235E-4 4.290645370793544 6.013518389339235E-4 40-41 0.0 0.0 6.013518389339235E-4 5.646393091670074 6.013518389339235E-4 42-43 0.0 0.0 6.013518389339235E-4 6.661474995790537 6.013518389339235E-4 44-45 0.0 0.0 6.013518389339235E-4 7.506073653573233 6.013518389339235E-4 46-47 0.0 0.0 6.013518389339235E-4 8.434260216967743 6.013518389339235E-4 48-49 0.0 0.0 6.013518389339235E-4 9.375977196738267 6.013518389339235E-4 50-51 0.0 0.0 6.013518389339235E-4 10.258160344454334 6.013518389339235E-4 52-53 0.0 0.0 6.013518389339235E-4 10.96023861640969 6.013518389339235E-4 54-55 0.0 0.0 0.001202703677867847 11.635857407451951 6.013518389339235E-4 56-57 0.0 0.0 0.001202703677867847 12.304861328265941 6.013518389339235E-4 58-59 0.0 0.0 0.001202703677867847 12.935679407307628 6.013518389339235E-4 60-61 0.0 0.0 0.001202703677867847 13.606788059557886 6.013518389339235E-4 62-63 0.0 0.0 0.001202703677867847 14.204531787458206 6.013518389339235E-4 64-65 0.0 0.0 0.001202703677867847 14.72680585957232 6.013518389339235E-4 66-67 0.0 0.0 0.001202703677867847 15.25599547783417 6.013518389339235E-4 68-69 0.0 0.0 0.001202703677867847 15.883806797681187 6.013518389339235E-4 70-71 0.0 0.0 0.001202703677867847 16.620462800375243 6.013518389339235E-4 72-73 0.0 0.0 0.001202703677867847 17.325848507444736 6.013518389339235E-4 74-75 0.0 0.0 0.001202703677867847 18.018004474057683 6.013518389339235E-4 76-77 0.0 0.0 0.001202703677867847 18.66024823803911 6.013518389339235E-4 78-79 0.0 0.0 0.001202703677867847 19.35631299160513 6.013518389339235E-4 80-81 0.0 0.0 0.001202703677867847 20.098080484930122 6.013518389339235E-4 82-83 0.0 0.0 0.001202703677867847 20.820604719409232 6.013518389339235E-4 84-85 0.0 0.0 0.001202703677867847 21.54042287061314 6.013518389339235E-4 86-87 0.0 0.0 0.001202703677867847 22.18958218074231 6.013518389339235E-4 88-89 0.0 0.0 0.001202703677867847 22.83242729656267 6.013518389339235E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTTAC 25 0.00503655 56.93468 7 TATATGG 40 5.405281E-4 47.445564 2 GGGAAAT 40 5.405281E-4 47.445564 9 GTGCAAA 45 9.641342E-4 42.186523 1 GTATATG 45 9.641342E-4 42.186523 1 GGGTGGT 70 4.3218606E-6 40.667625 8 ATGGGGT 125 1.3460522E-10 37.95645 5 TGGGGTG 85 1.6366483E-5 33.490986 6 GGTACTG 30 0.0039516613 31.65894 30-31 ATCACTA 30 0.0039662444 31.635132 76-77 AATTCAT 30 0.003969166 31.630377 12-13 GGTGGTA 75 3.0879152E-4 31.630375 9 ATATGGG 80 4.5074732E-4 29.653479 3 CTTCTGG 65 0.005854035 29.206053 1 GCCAAGA 85 6.426924E-4 27.909155 2 CTTTACA 105 6.91062E-5 27.119907 1 CTGTTGG 35 0.008430163 27.111752 58-59 CGTACCG 35 0.008430163 27.111752 56-57 CCAAGAC 70 0.008418254 27.111752 3 TACTAAA 35 0.008430163 27.111752 10-11 >>END_MODULE