FastQCFastQC Report
Fri 27 May 2016
SRR939817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939817_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440272
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49030.20092022528636153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37710.15453195381498455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42400.039.3901561
GTATCAA71550.037.1354981
TCAACGC87100.030.4543324
ATCAACG88550.030.0622043
TATCAAC88750.029.9391482
CAACGCA88900.029.6248075
AACGCAG90800.029.0055036
TACATGG72000.026.2283122
GTACATG70750.025.6852151
ACGCAGA102550.025.4970057
CGCAGAG105050.024.7993688
ACATGGG75100.024.5785473
GCAGAGT113450.021.875239
CATGGGG48950.021.2298474
GAGTACT62750.020.83269512-13
AGAGTAC91050.020.45447710-11
GTATAGG7050.020.1903111
GTAAGGT11550.020.1311954
TAAGGTG12650.019.5064015
GGACCGT3204.0199666E-1019.2782086
Produced by FastQC (version 0.11.5)