FastQCFastQC Report
Fri 27 May 2016
SRR939817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440272
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55560.22767953736304805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45380.18596287626953062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.13297697961538713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.041.523761
GTATCAA64700.031.7633611
TACATGG71500.025.838712
GTACATG72650.025.8221071
ATCAACG80400.025.3221573
TCAACGC80950.025.1495934
TATCAAC81450.025.1124692
CAACGCA81650.024.8762655
ACATGGG71050.024.475713
AACGCAG86450.023.5379816
CGCAGAG97400.020.5048878
ACGCAGA98750.020.4153637
TAAGGTG12650.019.744655
GTATAGG8900.019.0658741
GAGTACT64800.018.53617312-13
CATGGGG47300.018.5317274
AGAGTAC93250.018.51294910-11
GCAGAGT108450.018.2419059
GAGTACA54150.017.6702141
GTACTTT68250.017.66837914-15
Produced by FastQC (version 0.11.5)