##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939817_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2440272 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.312469265721198 31.0 31.0 34.0 30.0 34.0 2 31.5318501380174 31.0 31.0 34.0 30.0 34.0 3 31.590512041280643 31.0 31.0 34.0 30.0 34.0 4 35.4162990846922 37.0 35.0 37.0 33.0 37.0 5 35.131936521830355 37.0 35.0 37.0 32.0 37.0 6 35.15246906902181 37.0 35.0 37.0 32.0 37.0 7 35.07159365841185 37.0 35.0 37.0 32.0 37.0 8 35.00002704616534 37.0 35.0 37.0 32.0 37.0 9 36.599093461712464 39.0 35.0 39.0 32.0 39.0 10-11 36.44433653297665 38.0 35.0 39.0 32.0 39.0 12-13 36.37868708898024 38.0 35.0 39.0 32.0 39.0 14-15 37.40652046165346 39.0 36.0 41.0 32.0 41.0 16-17 37.31836102696748 39.0 36.0 40.5 32.0 41.0 18-19 37.27683922120157 39.0 36.0 40.0 31.5 41.0 20-21 37.181264629516704 39.0 36.0 40.0 31.0 41.0 22-23 37.04275814335451 39.0 36.0 40.0 31.0 41.0 24-25 36.86873307565714 39.0 36.0 40.0 30.5 41.0 26-27 36.55692767035806 38.0 35.0 40.0 30.0 41.0 28-29 36.3186890641699 38.0 35.0 40.0 30.0 41.0 30-31 36.05938600287181 38.0 34.5 40.0 29.5 41.0 32-33 36.02206475343732 38.0 34.5 40.0 29.5 41.0 34-35 36.117184887586305 38.0 35.0 40.0 30.0 41.0 36-37 36.0879637597776 38.0 35.0 40.0 30.0 41.0 38-39 35.94617280368746 38.0 35.0 40.0 29.0 41.0 40-41 35.765100775651234 38.0 34.5 40.0 28.5 41.0 42-43 35.56592994551427 38.0 34.0 40.0 27.5 41.0 44-45 35.32891497341279 38.0 34.0 40.0 27.0 41.0 46-47 35.04410737819391 38.0 33.5 40.0 26.5 41.0 48-49 34.735702208606256 38.0 33.0 40.0 25.5 41.0 50-51 34.453683032055444 38.0 33.0 40.0 24.5 41.0 52-53 34.15118867896693 37.0 33.0 40.0 24.0 41.0 54-55 33.81857842076621 37.0 33.0 40.0 23.5 41.0 56-57 32.46893829868146 36.5 31.0 39.5 16.0 40.5 58-59 28.974662045870296 34.0 23.0 38.0 7.0 40.0 60-61 28.967452398748993 33.5 24.0 38.0 7.5 40.0 62-63 30.79827822472249 34.5 28.5 38.0 8.0 40.0 64-65 30.266092468380574 34.5 28.0 38.0 2.0 40.0 66-67 24.6866201800455 28.0 17.0 35.5 2.0 39.0 68-69 16.985204723080052 13.0 7.0 28.5 2.0 35.0 70-71 15.838579879619976 12.5 7.5 25.5 2.0 33.0 72-73 17.830256217339706 16.0 7.0 29.0 2.0 34.0 74-75 18.559991877954587 17.5 7.5 30.0 2.0 34.5 76-77 19.695058993423686 22.5 7.5 30.5 2.0 34.5 78-79 22.78864056957585 27.5 11.0 33.5 2.0 35.5 80-81 25.436491096074533 31.0 20.0 34.5 2.0 36.0 82-83 26.38828868257309 32.0 25.0 35.0 2.0 36.0 84-85 26.370774241559957 33.0 25.0 35.0 2.0 36.0 86-87 26.065077786410697 32.5 24.0 35.0 2.0 35.0 88-89 25.59249050925471 32.0 21.5 34.5 2.0 35.0 90-91 23.94409393706931 31.5 10.0 34.0 2.0 35.0 92-93 21.770243644970726 30.0 2.0 34.0 2.0 35.0 94-95 17.270366172295546 19.0 2.0 32.5 2.0 35.0 96-97 15.892818300582887 10.0 2.0 31.5 2.0 34.0 98-99 14.756913573568848 2.0 2.0 31.0 2.0 34.0 100-101 14.104150275051307 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.27694414658731503 1101 2 0.23737216923039028 1101 3 0.3013405785264922 1101 4 0.1860625556004365 1101 5 0.37579682123645597 1101 6 0.32181226127742235 1101 7 0.3541289116778543 1101 8 0.3922087089664572 1101 9 0.37596306467745677 1101 10-11 0.4033059924586908 1101 12-13 0.44565440477764184 1101 14-15 0.5627080849212618 1101 16-17 0.3657872481873312 1101 18-19 0.47538015469320527 1101 20-21 0.4741529916008673 1101 22-23 0.5275051894044651 1101 24-25 0.5162480119053825 1101 26-27 0.5020749870067718 1101 28-29 0.5217056469317995 1101 30-31 0.7189178543002868 1101 32-33 0.7900952802102168 1101 34-35 0.5596108539141582 1101 36-37 0.5098034321371685 1101 38-39 0.42177937767701934 1101 40-41 0.37283994204404536 1101 42-43 0.05680686786624989 1101 44-45 0.1146916899560253 1101 46-47 -0.03702244290185064 1101 48-49 -0.1729227800269726 1101 50-51 -0.1033885634727909 1101 52-53 -0.037503541943536334 1101 54-55 -0.05296050105783223 1101 56-57 0.7555131556944801 1101 58-59 -8.320879229140829 1101 60-61 -9.73935926500765 1101 62-63 -3.1349393268163404 1101 64-65 -0.6164647337726663 1101 66-67 -1.9969580695415026 1101 68-69 -4.33881730896629 1101 70-71 -4.360557937417335 1101 72-73 -6.725395979024913 1101 74-75 -7.531694470714388 1101 76-77 -8.874020820037707 1101 78-79 -11.75984615434867 1101 80-81 -8.574676127806658 1101 82-83 -4.629544013032788 1101 84-85 -3.745910783670144 1101 86-87 -3.7014290273226536 1101 88-89 -3.918026522722318 1101 90-91 -9.191977887303775 1101 92-93 -14.658219417135411 1101 94-95 -12.592304682836177 1101 96-97 -12.404874223800372 1101 98-99 -11.66464194684081 1101 100-101 -11.217711935107005 1102 1 0.4465579677255249 1102 2 0.4246181041897401 1102 3 0.3591403752744604 1102 4 0.3824294255191347 1102 5 0.43362202448848564 1102 6 0.39561815558636226 1102 7 0.3941695620843646 1102 8 0.4102981398607639 1102 9 0.6782242435392405 1102 10-11 0.6181687973367431 1102 12-13 0.5853774738833337 1102 14-15 0.6886989385797975 1102 16-17 0.7158888742035856 1102 18-19 0.7998974311159728 1102 20-21 0.8217647802187571 1102 22-23 0.8089076284288552 1102 24-25 0.7082957761330206 1102 26-27 0.7102431780636849 1102 28-29 0.6987254640049727 1102 30-31 0.6843904152758995 1102 32-33 0.5542721094785108 1102 34-35 0.5892475409060225 1102 36-37 0.6820087166900137 1102 38-39 0.5752600687339253 1102 40-41 0.5946641290359125 1102 42-43 0.7271193678662442 1102 44-45 0.5764803078422105 1102 46-47 0.49915641888676276 1102 48-49 0.5890436833876649 1102 50-51 0.5270230218930649 1102 52-53 0.5410152588694714 1102 54-55 0.39752222251939884 1102 56-57 0.9085111231741578 1102 58-59 -9.058823842961967 1102 60-61 -10.378764752812529 1102 62-63 -3.192027741450488 1102 64-65 -0.40118272157754475 1102 66-67 -1.6488000410862185 1102 68-69 -3.8756313333565338 1102 70-71 -4.268395843921402 1102 72-73 -6.854676974959872 1102 74-75 -7.837131462584308 1102 76-77 -8.838693076135268 1102 78-79 -11.836129182803955 1102 80-81 -8.394963221302593 1102 82-83 -4.462432732544983 1102 84-85 -3.5032024503368113 1102 86-87 -3.3295794338267157 1102 88-89 -3.5237810958930496 1102 90-91 -9.082856952344425 1102 92-93 -14.223171144777687 1102 94-95 -11.966504377958127 1102 96-97 -11.747379305101184 1102 98-99 -11.306816743588778 1102 100-101 -11.027709394456599 1103 1 0.15217906251213975 1103 2 0.24190357378034122 1103 3 0.19620642916941833 1103 4 0.1447946278061707 1103 5 0.180062399871467 1103 6 0.14441673704398994 1103 7 0.20398054372533636 1103 8 0.2727972351781318 1103 9 0.22564469177933688 1103 10-11 0.2948892021520706 1103 12-13 0.3087463310882228 1103 14-15 0.4017628574237335 1103 16-17 0.4370038653980117 1103 18-19 0.45185456122140266 1103 20-21 0.4039687680598192 1103 22-23 0.36690924479516696 1103 24-25 0.30547588035246775 1103 26-27 0.18240850580004775 1103 28-29 0.2742139926291358 1103 30-31 0.30439324994816275 1103 32-33 0.2565826689342572 1103 34-35 0.3130434083157425 1103 36-37 0.14075669623212406 1103 38-39 0.10476961012014385 1103 40-41 0.11524535747430775 1103 42-43 0.18657782483953866 1103 44-45 0.07491671468401506 1103 46-47 0.051812868670850776 1103 48-49 -0.14661528744537122 1103 50-51 -0.06577419650938765 1103 52-53 0.0489779738823799 1103 54-55 -0.05894776614191244 1103 56-57 0.5667334821039773 1103 58-59 -6.894696922019168 1103 60-61 -9.126806718024465 1103 62-63 -3.0069565127708415 1103 64-65 -0.6442667243760276 1103 66-67 -2.1196104557927384 1103 68-69 -4.584381725385677 1103 70-71 -5.019041184202697 1103 72-73 -7.874284764880501 1103 74-75 -8.593001345096187 1103 76-77 -9.26537065930576 1103 78-79 -12.17112984623789 1103 80-81 -8.953071590220109 1103 82-83 -5.215271325100048 1103 84-85 -4.38869948297776 1103 86-87 -4.109428532762813 1103 88-89 -4.269196465847937 1103 90-91 -9.521679296799324 1103 92-93 -14.897847569954402 1103 94-95 -12.579150936545375 1103 96-97 -12.060985128844882 1103 98-99 -11.588999019046547 1103 100-101 -11.212696789211853 1104 1 0.058962174549048285 1104 2 -0.06424206918020658 1104 3 0.028214658732526487 1104 4 0.13175102138409756 1104 5 -0.07415718906523949 1104 6 0.08602537753937867 1104 7 0.09174848489345777 1104 8 0.08663475753157712 1104 9 0.06144181743683674 1104 10-11 0.21402389164927627 1104 12-13 0.14699155525593 1104 14-15 0.04490777219130848 1104 16-17 0.17442411352949705 1104 18-19 0.10213006271381175 1104 20-21 -0.005183836949541387 1104 22-23 0.04846729389306148 1104 24-25 0.19252422603342012 1104 26-27 -0.001964058247089895 1104 28-29 -3.843549077870989E-4 1104 30-31 0.057671073579172116 1104 32-33 0.032787517149152734 1104 34-35 0.07534967841747431 1104 36-37 -0.051192704728173055 1104 38-39 0.027898307036842596 1104 40-41 0.11503778972615919 1104 42-43 0.1454784554306272 1104 44-45 0.020523183701428138 1104 46-47 -0.03205555548463224 1104 48-49 -0.11088497649496531 1104 50-51 -0.0986033353638831 1104 52-53 -0.2026605967632591 1104 54-55 -0.35141754667962033 1104 56-57 0.239081036489182 1104 58-59 -6.689126791686817 1104 60-61 -8.660171902976742 1104 62-63 -3.141826284137899 1104 64-65 -0.9781748607042324 1104 66-67 -2.1729479977975714 1104 68-69 -4.690098122064157 1104 70-71 -5.399195032707992 1104 72-73 -7.88977810742815 1104 74-75 -8.987926074835066 1104 76-77 -9.521821362716146 1104 78-79 -12.37550219739503 1104 80-81 -9.164256011912617 1104 82-83 -5.7899112407737725 1104 84-85 -4.963246600447182 1104 86-87 -4.770790690310143 1104 88-89 -4.852225612126293 1104 90-91 -10.152070326597373 1104 92-93 -15.037566593367934 1104 94-95 -12.36794508938511 1104 96-97 -11.819068944183005 1104 98-99 -11.157495147723813 1104 100-101 -10.709129107660353 1105 1 0.13287738729129472 1105 2 0.12702001228980464 1105 3 0.11864117730907608 1105 4 0.09206981707986017 1105 5 0.0804464838009622 1105 6 0.11502674784616573 1105 7 0.15779107920465663 1105 8 0.17481754845302788 1105 9 0.3107019656963743 1105 10-11 0.20182681752154963 1105 12-13 0.1701534205907862 1105 14-15 0.23454255958051817 1105 16-17 0.31425390162123534 1105 18-19 0.30641553579682324 1105 20-21 0.3476800195220022 1105 22-23 0.347104567464541 1105 24-25 0.2710188369682385 1105 26-27 0.42783525066959527 1105 28-29 0.24184942918666508 1105 30-31 0.08728120946768314 1105 32-33 0.20874107219116667 1105 34-35 0.21750812631061223 1105 36-37 0.15444664372906658 1105 38-39 0.302091919029408 1105 40-41 0.20929165899521252 1105 42-43 0.2067938418638633 1105 44-45 0.30541883035565576 1105 46-47 0.2025567551926315 1105 48-49 0.08032627713856044 1105 50-51 0.17527815281673753 1105 52-53 0.18248682294994012 1105 54-55 -0.11286315753527276 1105 56-57 0.6398596225464033 1105 58-59 -6.808378608855001 1105 60-61 -8.654342475937913 1105 62-63 -2.85310832575772 1105 64-65 -0.3291158767526845 1105 66-67 -1.429844590316943 1105 68-69 -4.065169945594583 1105 70-71 -4.573153668196747 1105 72-73 -5.863148848880677 1105 74-75 -6.569249882914997 1105 76-77 -8.523917645828366 1105 78-79 -11.775208359919663 1105 80-81 -7.983307818623121 1105 82-83 -4.310850045519224 1105 84-85 -3.408700663913624 1105 86-87 -3.2667045996397093 1105 88-89 -3.1822904088543815 1105 90-91 -8.36164466975713 1105 92-93 -11.44403534111318 1105 94-95 -11.507065624678178 1105 96-97 -11.785487810541142 1105 98-99 -11.288085442937145 1105 100-101 -10.799317772410168 1106 1 0.02596644562486361 1106 2 0.10495977259218847 1106 3 -0.013790487625062298 1106 4 -0.018626427048339167 1106 5 0.049958065644752025 1106 6 -0.011638413115967694 1106 7 -0.01719073045308761 1106 8 0.11847616186059184 1106 9 0.06589587950733033 1106 10-11 0.055974336636545274 1106 12-13 0.15185378291238294 1106 14-15 0.13567007827899147 1106 16-17 -0.07201775384601206 1106 18-19 0.08439423562041526 1106 20-21 0.006600971809618272 1106 22-23 0.005472181406638299 1106 24-25 0.03895244799051767 1106 26-27 0.17415565157231327 1106 28-29 -0.086540210123907 1106 30-31 0.027720128259893784 1106 32-33 0.029295666546133248 1106 34-35 0.22475399306286903 1106 36-37 0.30757945897734373 1106 38-39 0.2060188212177536 1106 40-41 0.2298283011331037 1106 42-43 0.27665169808178547 1106 44-45 0.14476150153254963 1106 46-47 0.2041324981310879 1106 48-49 0.23171198003809224 1106 50-51 0.17381719428781395 1106 52-53 0.06263858053172555 1106 54-55 -0.2298951458999099 1106 56-57 0.47848743385633696 1106 58-59 -6.7620818267414755 1106 60-61 -9.224680618538894 1106 62-63 -2.97537454777337 1106 64-65 -0.5593325577526045 1106 66-67 -1.7979584338494874 1106 68-69 -4.268918891587951 1106 70-71 -4.625298643327598 1106 72-73 -6.35948228612807 1106 74-75 -7.020196741285082 1106 76-77 -8.562815596030658 1106 78-79 -11.797784806578575 1106 80-81 -8.048521991640193 1106 82-83 -4.222489781976801 1106 84-85 -3.366744540617411 1106 86-87 -3.2045519092529773 1106 88-89 -3.2349335902972136 1106 90-91 -8.647174359446922 1106 92-93 -12.20943497732248 1106 94-95 -11.68929596486139 1106 96-97 -11.866119902938232 1106 98-99 -11.30197589017821 1106 100-101 -11.00267385221423 1107 1 0.04933741423920068 1107 2 0.10690213638966739 1107 3 -0.01437678600552772 1107 4 -0.004562444399567767 1107 5 0.07265642714778409 1107 6 0.1252348803250385 1107 7 0.03750329591431267 1107 8 0.008341713892569658 1107 9 -0.07800347883650005 1107 10-11 0.0935512716048379 1107 12-13 0.020790385894223107 1107 14-15 -3.614963595310883E-4 1107 16-17 0.06006064720210702 1107 18-19 0.12845384106430657 1107 20-21 0.005382466149306708 1107 22-23 0.165210443920067 1107 24-25 0.13031307758682686 1107 26-27 0.2957351881561934 1107 28-29 0.29653374627498863 1107 30-31 0.2038675669439698 1107 32-33 0.186452012558334 1107 34-35 0.1551542652442066 1107 36-37 0.11229727450169946 1107 38-39 -9.474285627177892E-4 1107 40-41 -0.0765802139493843 1107 42-43 0.0028582841224036315 1107 44-45 -0.02011947588962215 1107 46-47 -0.08382047245850544 1107 48-49 -0.10237167165259109 1107 50-51 0.10038298825134717 1107 52-53 0.08930013211229237 1107 54-55 -0.15611628923517884 1107 56-57 0.4962459143151676 1107 58-59 -6.82790149925577 1107 60-61 -9.2150006853689 1107 62-63 -3.0534789409378504 1107 64-65 -0.6371723487151932 1107 66-67 -1.9647818585398582 1107 68-69 -4.366295757242153 1107 70-71 -4.954962186185808 1107 72-73 -6.411364267202252 1107 74-75 -6.8904075249015815 1107 76-77 -9.318091530218332 1107 78-79 -12.52047577257527 1107 80-81 -8.855523315819793 1107 82-83 -4.9435603307667755 1107 84-85 -3.9550907918803198 1107 86-87 -3.6959076808530327 1107 88-89 -3.6645346302756856 1107 90-91 -8.716993281392444 1107 92-93 -11.948067015657578 1107 94-95 -11.788043085956046 1107 96-97 -12.202672869120569 1107 98-99 -11.541345559320286 1107 100-101 -10.91769397533689 1108 1 -0.02426633387074162 1108 2 0.02494840771682405 1108 3 -0.062418912387126824 1108 4 0.04815102336244337 1108 5 -0.21138577771799305 1108 6 -0.028664618234650163 1108 7 0.0067988145904820385 1108 8 -0.029704410276757187 1108 9 -0.01708289623865511 1108 10-11 0.013645642695188087 1108 12-13 -0.10298069293209977 1108 14-15 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3.3548849906309215 2312 82-83 2.0636317750857778 2312 84-85 1.6662775798063727 2312 86-87 1.4756652957117637 2312 88-89 1.6499012121432948 2312 90-91 3.0783569845877103 2312 92-93 4.994129631470468 2312 94-95 8.871037206764424 2312 96-97 9.589744747563605 2312 98-99 9.944573702493528 2312 100-101 9.246365479487318 2313 1 -0.2677441485780818 2313 2 -0.26264373407749986 2313 3 -0.3229517242724924 2313 4 -0.34031308053187814 2313 5 -0.3313596673131656 2313 6 -0.3219297870686333 2313 7 -0.4369016226732896 2313 8 -0.4097633012116617 2313 9 -0.44335150275257007 2313 10-11 -0.45385844774487083 2313 12-13 -0.3985687186320277 2313 14-15 -0.5072267115163953 2313 16-17 -0.4140791640264041 2313 18-19 -0.45083644836022785 2313 20-21 -0.46886545623628706 2313 22-23 -0.5105037164479498 2313 24-25 -0.5203534511989432 2313 26-27 -0.5734299430186738 2313 28-29 -0.6154246011598019 2313 30-31 -0.5267325909923386 2313 32-33 -0.23934174269054154 2313 34-35 -0.2987900684776932 2313 36-37 -0.6103778655727581 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4.168341472427471 2314 62-63 1.707028406441033 2314 64-65 1.5882010071439048 2314 66-67 2.0342703691849167 2314 68-69 0.014714388083969965 2314 70-71 2.828242662342129 2314 72-73 8.739593062309435 2314 74-75 10.427301129994564 2314 76-77 9.30915683694419 2314 78-79 6.882265782288879 2314 80-81 4.000014299779114 2314 82-83 2.4077112961835674 2314 84-85 2.127579927179333 2314 86-87 2.0927205485730305 2314 88-89 2.2169136200172694 2314 90-91 3.3788123234824 2314 92-93 5.498643595587719 2314 94-95 9.23330595616876 2314 96-97 10.039586704798184 2314 98-99 10.273573333574433 2314 100-101 9.446542440446864 2315 1 -0.7241718941335549 2315 2 -0.47281234720928467 2315 3 -0.4350687453144779 2315 4 -0.33373426996016775 2315 5 -0.5750028073231803 2315 6 -0.5290307879728218 2315 7 -0.5962528464731918 2315 8 -0.7918640658306231 2315 9 -0.994690137276045 2315 10-11 -0.9270803227153337 2315 12-13 -0.7232424087455414 2315 14-15 -0.7602176098097857 2315 16-17 -0.884444902039661 2315 18-19 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1.3725094770259645 2315 86-87 1.334217210124038 2315 88-89 1.289554686866346 2315 90-91 2.4745963491799223 2315 92-93 4.472147767140282 2315 94-95 8.418659901356971 2315 96-97 9.225432988333347 2315 98-99 9.234221376862585 2315 100-101 8.17629751426705 2316 1 -0.4544326193939412 2316 2 -0.45539342735654387 2316 3 -0.7276612923645409 2316 4 -0.4703268869789454 2316 5 -0.5706100188810481 2316 6 -0.6476804125231084 2316 7 -0.717954596505102 2316 8 -0.7157819190934092 2316 9 -0.8262194958205171 2316 10-11 -0.8109481864474404 2316 12-13 -0.7655147709812056 2316 14-15 -1.0419529690723053 2316 16-17 -1.0886032260048637 2316 18-19 -1.1583575184079251 2316 20-21 -1.0179586673724117 2316 22-23 -1.0473147154773343 2316 24-25 -2.4104510893675197 2316 26-27 -1.602774248900353 2316 28-29 -1.0646226419897218 2316 30-31 -1.1485605517704869 2316 32-33 -1.0181637420306515 2316 34-35 -1.018306730713057 2316 36-37 -1.0112598284587264 2316 38-39 -1.2221572743022762 2316 40-41 -1.1076481506961997 2316 42-43 -1.3261328352390862 2316 44-45 -1.2254223120022871 2316 46-47 -1.066607000182593 2316 48-49 -1.2763598866153103 2316 50-51 -1.2308666198443134 2316 52-53 -1.2687939759203175 2316 54-55 -1.1891696736693902 2316 56-57 -0.0035641628116067636 2316 58-59 3.3929275532762944 2316 60-61 3.5974216658917797 2316 62-63 0.8563422335417492 2316 64-65 0.7819627123736481 2316 66-67 5.72505746584806 2316 68-69 8.258130253629876 2316 70-71 5.091425075941176 2316 72-73 8.960324270310661 2316 74-75 9.875781225187136 2316 76-77 8.324743648769118 2316 78-79 6.044793699127343 2316 80-81 2.9165020527081005 2316 82-83 1.4525979386387746 2316 84-85 1.1985810158822439 2316 86-87 1.2955093556679742 2316 88-89 1.1939080572888692 2316 90-91 2.700168304471301 2316 92-93 4.3986698768217884 2316 94-95 8.461306180571292 2316 96-97 9.172175450979315 2316 98-99 9.276096268294175 2316 100-101 8.276237174908383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 21.0 4 60.0 5 134.0 6 272.0 7 672.0 8 1532.0 9 2672.0 10 4316.0 11 6390.0 12 9067.0 13 12469.0 14 16485.0 15 22546.0 16 30033.0 17 38518.0 18 43737.0 19 46284.0 20 48795.0 21 48628.0 22 46487.0 23 44978.0 24 45457.0 25 49094.0 26 63420.0 27 91805.0 28 128644.0 29 166543.0 30 192183.0 31 203067.0 32 206829.0 33 208812.0 34 206508.0 35 192141.0 36 152779.0 37 85246.0 38 22854.0 39 790.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.57292133753811 19.791693137784524 14.575209261871835 24.060176262805534 2 16.03380472859665 20.697404894489317 38.812285564879275 24.45650481203476 3 19.55258658702305 23.916238150580853 30.071256337584888 26.459918924811205 4 13.488742238570126 16.849842968324843 36.45970613111981 33.20170866198522 5 13.609947855438561 37.49996412165506 34.60054280860604 14.289545214300329 6 32.96855144367162 36.519673429713336 16.640446901184337 13.871328225430702 7 28.68323695063501 32.321847728449946 20.567010562756938 18.427904758158107 8 26.425949936646305 35.90474488867616 19.19035122371805 18.478953950959482 9 25.948828655166313 16.337932820603605 19.763616514880308 37.949622009349774 10-11 25.170595736868677 26.44840411232846 27.411882773723583 20.96911737707928 12-13 25.76796766917786 23.425503386507735 27.893386474950333 22.91314246936407 14-15 22.360554136311368 25.213788168994693 25.74336158435187 26.68229611034207 16-17 22.384492384455505 28.4021617262338 26.516511274153043 22.696834615157655 18-19 22.373521123643805 27.096827995776646 28.441532070929927 22.088118809649625 20-21 22.735395890294196 26.329995180865083 28.675020653435357 22.259588275405363 22-23 22.41825911209898 26.221974435636685 28.626050702544635 22.733715749719703 24-25 22.492369703049498 26.379907649639055 28.61746559399936 22.510257053312092 26-27 22.22761325453593 26.77934971446766 28.40857750577652 22.584459525219888 28-29 22.172982622072908 27.12460177803495 28.362091645760103 22.340323954132042 30-31 22.381070351976064 26.698801036775023 28.29084781091007 22.629280800338844 32-33 22.232649151474483 26.60965777247325 28.353937332561557 22.80375574349071 34-35 22.705951270846537 26.77792195737843 28.17966731598711 22.336459455787917 36-37 22.783894359894404 26.46991131417057 28.282567443797436 22.463626882137593 38-39 22.340433380798146 26.771999203181373 28.249249293561263 22.638318122459218 40-41 22.63447313877879 26.61628767997265 28.345209736758452 22.404029444490103 42-43 22.425825751231642 26.607981466019194 28.19070292302881 22.77548985972036 44-45 22.4823117147051 26.712251840356384 28.285926146361977 22.519510298576535 46-47 22.699255483243146 26.86562667421991 27.82815325166044 22.6069645908765 48-49 22.51279734772714 26.9610849133945 27.821401166108096 22.704716572770263 50-51 22.435116151194016 27.08930020312036 27.84038031115883 22.635203334526793 52-53 22.694251036207046 26.907220403416282 27.732374233411722 22.666154326964953 54-55 22.328901166120716 26.959161696402866 27.883840810843765 22.828096326632654 56-57 22.44379494465367 26.929098507149078 27.9205637680071 22.706542780190148 58-59 21.193854557925977 26.530801027199903 29.494892598061274 22.780451816812853 60-61 22.389487021655068 26.702229219182406 28.278867684491445 22.629416074671084 62-63 22.18276383210997 26.945732101116924 28.04940367107821 22.822100395694893 64-65 21.475816643947198 26.8908544209183 28.481819056732043 23.151509878402457 66-67 22.13707593566791 26.167955606315797 29.149757172667467 22.545211285348827 68-69 23.204702624442323 24.97218977696201 29.997476011575795 21.825631587019874 70-71 22.10505206544532 25.506923793739826 29.917477741244173 22.470546399570676 72-73 21.866197639071817 25.73477717638537 29.82736459376354 22.57166059077927 74-75 22.258343290048426 25.977584951650854 29.040464131779647 22.723607626521066 76-77 20.54059352308151 26.51134280312803 29.5019602504031 23.446103423387356 78-79 21.410826857613458 26.81604483155857 28.266769339803773 23.506358971024195 80-81 21.877524057818263 26.929266346674357 27.442734749926956 23.75047484558042 82-83 22.055719665389418 26.922661615858413 27.3154256118023 23.70619310694987 84-85 21.961791113278448 26.926549111368825 27.211497088436936 23.90016268691579 86-87 22.01192770106737 26.85277694223675 27.17486093364402 23.96043442305186 88-89 21.912643736567837 26.90860422702966 27.044080955746114 24.134671080656396 90-91 21.71241105258294 26.768895871196953 27.402499255562745 24.116193820657365 92-93 21.451689843532552 26.609524341251262 27.632819164614723 24.30596665060146 94-95 20.494938179447274 26.139758365682404 29.104196363961044 24.261107090909277 96-97 20.251484243671527 25.96804119280936 29.265047917034735 24.51542664648437 98-99 20.492685183939184 26.16086280980443 28.513652977631658 24.832799028624724 100-101 20.22585553771542 26.287616871846488 28.443959532981705 25.042568057456382 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 171.0 1 125.5 2 98.5 3 132.0 4 155.5 5 169.5 6 243.0 7 394.5 8 555.0 9 701.0 10 797.5 11 905.5 12 1075.5 13 1261.0 14 1444.0 15 1648.5 16 1877.5 17 2126.5 18 2467.5 19 2874.0 20 3426.0 21 4197.0 22 5031.5 23 6220.0 24 7887.0 25 10032.5 26 12852.0 27 15982.0 28 19296.5 29 23192.5 30 27593.0 31 32482.0 32 37956.5 33 43411.5 34 48316.0 35 52896.5 36 57781.0 37 62626.0 38 66584.0 39 70258.0 40 74218.0 41 78320.0 42 82226.5 43 85728.0 44 88797.0 45 91263.0 46 93248.0 47 95311.0 48 96996.5 49 96569.5 50 95087.0 51 93401.5 52 91191.5 53 88606.0 54 84492.5 55 78678.5 56 71750.5 57 63944.5 58 55700.0 59 47140.0 60 38602.0 61 31145.0 62 24591.5 63 18402.0 64 13304.5 65 9744.5 66 7145.5 67 5233.5 68 3802.5 69 2689.5 70 1860.5 71 1249.5 72 892.5 73 638.0 74 438.5 75 274.0 76 174.0 77 115.0 78 63.0 79 28.0 80 19.0 81 10.0 82 6.5 83 6.0 84 4.5 85 3.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020735393431551893 2 0.005737065376318706 3 0.0027865746113547997 4 0.0 5 0.06044408164335779 6 4.097903840227647E-5 7 0.0 8 1.6391615360910588E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.458742304136588E-4 16-17 0.0 18-19 0.0013318187480739852 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.2538471121252057E-4 28-29 0.002048951920113823 30-31 0.008421192391667815 32-33 3.8930086482162645E-4 34-35 0.0011679025944648792 36-37 0.024280080253348806 38-39 0.023358051889297588 40-41 0.025837283712635314 42-43 0.026431479769468323 44-45 0.027169102460709298 46-47 0.028808263996800355 48-49 0.028849243035202633 50-51 0.02399322698453287 52-53 0.01985434410590295 54-55 0.014117278729584244 56-57 0.14445111036802455 58-59 0.7678447320626552 60-61 0.30609292734580407 62-63 3.2783230721821177E-4 64-65 0.15033160237875123 66-67 1.4369299815758243 68-69 2.7473986506422237 70-71 2.220592622461758 72-73 1.9007307382127894 74-75 1.582876826845532 76-77 1.124567261354472 78-79 0.4325337503360281 80-81 2.0489519201138232E-4 82-83 1.0244759600569116E-4 84-85 8.195807680455294E-5 86-87 0.002868532688159353 88-89 2.0489519201138236E-5 90-91 0.22731072601742758 92-93 0.17891448166433907 94-95 1.3233975556823174 96-97 1.3068420241677976 98-99 1.0301925359140292 100-101 0.41167541978926936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2440272.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.452471792692556 #Duplication Level Percentage of deduplicated Percentage of total 1 74.69144616268359 41.41825311491626 2 13.909611095327678 15.426446338219629 3 4.959066278741754 8.249774488200599 4 2.1958995408199 4.870722293876081 5 1.217112323260391 3.3745943387067663 6 0.7133498007609212 2.37342058230107 7 0.47492190983242827 1.8434915666100085 8 0.3255954240292404 1.444405685344898 9 0.23562596325236054 1.175943787278978 >10 1.1679215588958374 11.58503100310676 >50 0.07321503847331592 2.7724273276790643 >100 0.03275109211445433 3.242740957751241 >500 0.0022978333202446216 0.9128242883955479 >1k 0.0011118548323740764 1.0815537419180181 >5k 7.41236554916051E-5 0.22837048569515098 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5556 0.22767953736304805 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4538 0.18596287626953062 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3245 0.13297697961538713 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.0979038402276464E-5 2 0.0 0.0 0.0 0.0 4.0979038402276464E-5 3 0.0 0.0 0.0 0.0 4.0979038402276464E-5 4 0.0 0.0 0.0 0.0 4.0979038402276464E-5 5 0.0 0.0 0.0 0.0 4.0979038402276464E-5 6 0.0 0.0 0.0 0.0 4.0979038402276464E-5 7 0.0 0.0 0.0 0.0 4.0979038402276464E-5 8 0.0 0.0 0.0 0.0 4.0979038402276464E-5 9 0.0 0.0 0.0 4.0979038402276464E-5 4.0979038402276464E-5 10-11 0.0 0.0 0.0 4.0979038402276464E-5 4.0979038402276464E-5 12-13 0.0 0.0 0.0 6.14685576034147E-5 8.195807680455293E-5 14-15 0.0 0.0 0.0 8.195807680455293E-5 1.229371152068294E-4 16-17 0.0 0.0 0.0 1.434266344079676E-4 1.229371152068294E-4 18-19 0.0 0.0 0.0 1.6391615360910586E-4 1.229371152068294E-4 20-21 0.0 0.0 0.0 2.458742304136588E-4 1.229371152068294E-4 22-23 0.0 0.0 0.0 3.688113456204882E-4 1.229371152068294E-4 24-25 0.0 0.0 0.0 6.556646144364234E-4 1.229371152068294E-4 26-27 0.0 0.0 0.0 0.0012088816328671558 1.434266344079676E-4 28-29 0.0 0.0 0.0 0.004446225666646997 1.6391615360910586E-4 30-31 0.0 0.0 0.0 0.016084272572893514 1.6391615360910586E-4 32-33 0.0 0.0 0.0 0.045998970606555334 1.6391615360910586E-4 34-35 0.0 0.0 0.0 0.09453864159405181 1.6391615360910586E-4 36-37 0.0 0.0 0.0 0.17940623012516638 1.6391615360910586E-4 38-39 0.0 0.0 0.0 0.3092073342643771 2.0489519201138235E-4 40-41 0.0 0.0 0.0 0.4506464853098343 2.0489519201138235E-4 42-43 0.0 0.0 0.0 0.5956917917346919 2.0489519201138235E-4 44-45 0.0 0.0 0.0 0.7785607506048506 2.0489519201138235E-4 46-47 0.0 0.0 0.0 0.9866318180924094 2.0489519201138235E-4 48-49 0.0 0.0 0.0 1.187429106263564 2.0489519201138235E-4 50-51 0.0 0.0 0.0 1.3916891231797113 2.0489519201138235E-4 52-53 0.0 4.0979038402276464E-5 0.0 1.5841676665552036 2.458742304136588E-4 54-55 0.0 4.0979038402276464E-5 0.0 1.8008443321072405 2.458742304136588E-4 56-57 4.0979038402276464E-5 4.0979038402276464E-5 0.0 1.985147557321479 2.868532688159353E-4 58-59 4.0979038402276464E-5 4.0979038402276464E-5 0.0 2.1080846725283084 2.868532688159353E-4 60-61 4.0979038402276464E-5 4.0979038402276464E-5 2.0489519201138232E-5 2.20172177527751 2.868532688159353E-4 62-63 1.229371152068294E-4 8.195807680455293E-5 4.0979038402276464E-5 2.2796229272802377 2.868532688159353E-4 64-65 1.229371152068294E-4 1.229371152068294E-4 4.0979038402276464E-5 2.3603311434135215 2.868532688159353E-4 66-67 1.434266344079676E-4 1.229371152068294E-4 4.0979038402276464E-5 2.4284178157189036 2.868532688159353E-4 68-69 1.6391615360910586E-4 1.229371152068294E-4 4.0979038402276464E-5 2.509740717428221 2.868532688159353E-4 70-71 1.6391615360910586E-4 1.229371152068294E-4 4.0979038402276464E-5 2.658351200194077 2.868532688159353E-4 72-73 1.844056728102441E-4 1.229371152068294E-4 4.0979038402276464E-5 2.848289043188628 2.868532688159353E-4 74-75 2.0489519201138235E-4 1.229371152068294E-4 4.0979038402276464E-5 3.0684284374856574 2.868532688159353E-4 76-77 2.0489519201138235E-4 1.229371152068294E-4 4.0979038402276464E-5 3.400891376043326 2.868532688159353E-4 78-79 2.0489519201138235E-4 1.229371152068294E-4 4.0979038402276464E-5 3.8734206678599765 2.868532688159353E-4 80-81 2.0489519201138235E-4 1.229371152068294E-4 4.0979038402276464E-5 4.402214179402952 2.868532688159353E-4 82-83 2.0489519201138235E-4 1.229371152068294E-4 4.0979038402276464E-5 4.902117468872323 2.868532688159353E-4 84-85 2.0489519201138235E-4 1.229371152068294E-4 4.0979038402276464E-5 5.301376240025702 3.073427880170735E-4 86-87 2.0489519201138235E-4 1.229371152068294E-4 4.0979038402276464E-5 5.655578558455779 3.278323072182117E-4 88-89 2.0489519201138235E-4 1.229371152068294E-4 4.0979038402276464E-5 6.016911229567851 3.278323072182117E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4200 0.0 41.52376 1 GTATCAA 6470 0.0 31.763361 1 TACATGG 7150 0.0 25.83871 2 GTACATG 7265 0.0 25.822107 1 ATCAACG 8040 0.0 25.322157 3 TCAACGC 8095 0.0 25.149593 4 TATCAAC 8145 0.0 25.112469 2 CAACGCA 8165 0.0 24.876265 5 ACATGGG 7105 0.0 24.47571 3 AACGCAG 8645 0.0 23.537981 6 CGCAGAG 9740 0.0 20.504887 8 ACGCAGA 9875 0.0 20.415363 7 TAAGGTG 1265 0.0 19.74465 5 GTATAGG 890 0.0 19.065874 1 GAGTACT 6480 0.0 18.536173 12-13 CATGGGG 4730 0.0 18.531727 4 AGAGTAC 9325 0.0 18.512949 10-11 GCAGAGT 10845 0.0 18.241905 9 GAGTACA 5415 0.0 17.670214 1 GTACTTT 6825 0.0 17.668379 14-15 >>END_MODULE