FastQCFastQC Report
Fri 27 May 2016
SRR939816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610970
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52750.20203219493138566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41420.1586383604560757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42700.040.200511
GTATCAA70800.037.0652961
ATCAACG86400.030.0475163
TCAACGC87000.029.7873744
TATCAAC88300.029.5069262
CAACGCA88150.029.4010285
AACGCAG91600.028.6542226
TACATGG80850.026.757942
GTACATG82300.026.6860081
ACGCAGA103200.025.1599227
ACATGGG83400.024.474113
CGCAGAG104900.024.3934548
GCAGAGT111000.021.44269
GAGTACT60900.020.19703712-13
CATGGGG53200.020.1568724
AGAGTAC94650.019.67959210-11
TAAGGTG13050.019.1028695
CAGAGTA105150.018.63147510-11
GTACTTT66050.018.41532114-15
GTAAGGT11500.017.995124
Produced by FastQC (version 0.11.5)