FastQCFastQC Report
Fri 27 May 2016
SRR939816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610970
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61420.23523824479025038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51050.19552120476298082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35400.13558179527148914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47700.039.6975361
GTATCAA74850.033.6041641
ATCAACG92950.026.5377033
TCAACGC93200.026.4665184
TATCAAC95050.026.2044352
CAACGCA95700.025.7255575
AACGCAG99150.024.8304186
TACATGG81300.023.7504272
GTACATG82250.023.5414561
ACATGGG81650.022.1930263
ACGCAGA115650.021.2057827
CGCAGAG116400.021.0283958
TAAGGTG13500.019.3258365
GTGTAGC16700.019.32421
GAGTACT68800.018.99586912-13
AGAGTAC108750.018.8003410-11
GCAGAGT128750.018.6797089
AGTACTT74350.018.27972412-13
GTACTTT72050.017.84273114-15
GTAAGGT12500.017.835994
Produced by FastQC (version 0.11.5)