##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939816_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2610970 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.283164494421612 31.0 31.0 34.0 30.0 34.0 2 31.52500564924147 31.0 31.0 34.0 30.0 34.0 3 31.56980700659142 31.0 31.0 34.0 30.0 34.0 4 35.39772460043585 37.0 35.0 37.0 33.0 37.0 5 35.11296491342298 37.0 35.0 37.0 32.0 37.0 6 35.13944549343731 37.0 35.0 37.0 32.0 37.0 7 35.03660133973198 37.0 35.0 37.0 32.0 37.0 8 34.99513514134593 37.0 35.0 37.0 32.0 37.0 9 36.57314216555533 39.0 35.0 39.0 32.0 39.0 10-11 36.42862020628349 38.0 35.0 39.0 32.0 39.0 12-13 36.34006020750908 38.0 35.0 39.0 32.0 39.0 14-15 37.35564349647832 39.0 36.0 41.0 32.0 41.0 16-17 37.26214797565656 39.0 36.0 40.5 32.0 41.0 18-19 37.215762915697994 39.0 36.0 40.0 31.0 41.0 20-21 37.116640175873336 39.0 36.0 40.0 31.0 41.0 22-23 36.992196195283746 39.0 36.0 40.0 31.0 41.0 24-25 36.83127726477133 39.0 35.5 40.0 30.5 41.0 26-27 36.51074275077845 38.0 35.0 40.0 30.0 41.0 28-29 36.27434861373359 38.0 35.0 40.0 30.0 41.0 30-31 35.99665775554679 38.0 34.0 40.0 29.0 41.0 32-33 35.98051758541845 38.0 34.0 40.0 29.0 41.0 34-35 35.83374780254082 38.0 34.0 40.0 29.0 41.0 36-37 35.895718449465136 38.0 34.5 40.0 29.0 41.0 38-39 35.792602557670136 38.0 34.0 40.0 29.0 41.0 40-41 35.710617318467854 38.0 34.0 40.0 28.5 41.0 42-43 35.488383244541296 38.0 34.0 40.0 27.0 41.0 44-45 35.251958467542714 38.0 34.0 40.0 27.0 41.0 46-47 34.98507949152997 38.0 33.5 40.0 26.0 41.0 48-49 34.68075236406393 38.0 33.0 40.0 25.5 41.0 50-51 34.45098277651601 38.0 33.0 40.0 24.5 41.0 52-53 34.185714696070804 37.0 33.0 40.0 24.0 41.0 54-55 33.89287199776328 37.0 32.5 40.0 23.5 41.0 56-57 33.50557149258705 37.0 32.0 39.5 23.0 41.0 58-59 33.116338180829345 36.0 31.0 39.0 22.0 40.0 60-61 32.78081440997024 36.0 31.0 39.0 22.0 40.0 62-63 32.471588719901035 35.5 31.0 39.0 20.5 40.0 64-65 32.00984825562914 35.0 30.5 38.5 19.5 40.0 66-67 31.050003638494506 34.5 29.0 38.0 13.0 40.0 68-69 29.045004538543147 34.0 26.0 37.0 7.5 39.0 70-71 28.367268103425165 33.0 24.5 36.0 7.5 39.0 72-73 28.948520281734375 33.0 26.0 36.0 7.5 39.0 74-75 29.074402425152336 33.0 26.5 36.0 8.0 38.5 76-77 28.706235613584223 32.5 27.0 35.0 7.0 37.0 78-79 29.266942362417034 33.5 28.5 35.0 7.0 37.0 80-81 29.085789763957457 33.5 28.5 35.0 6.0 37.0 82-83 28.622946069851437 33.0 27.5 35.0 2.0 36.5 84-85 28.24617881476999 33.0 27.0 35.0 2.0 36.0 86-87 27.833750100537348 33.0 26.0 35.0 2.0 35.5 88-89 27.34627513912454 32.5 26.0 35.0 2.0 35.0 90-91 26.822955261837556 32.0 25.0 34.0 2.0 35.0 92-93 26.289633163153923 32.0 24.5 34.0 2.0 35.0 94-95 25.408682022390146 31.0 21.0 34.0 2.0 35.0 96-97 24.823290386331514 31.0 19.5 34.0 2.0 35.0 98-99 24.09068851806034 31.0 10.0 34.0 2.0 35.0 100-101 22.55939114581937 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3138842909153112 1101 2 0.34224840275626534 1101 3 0.3000556134645187 1101 4 0.2537705169720397 1101 5 0.29784541734848347 1101 6 0.1844970730677673 1101 7 0.2780947341067517 1101 8 0.2334550667364539 1101 9 0.4070731183139742 1101 10-11 0.3506207180831922 1101 12-13 0.4211576484641384 1101 14-15 0.42109211755279574 1101 16-17 0.4535478796857362 1101 18-19 0.33292695883938705 1101 20-21 0.2878609773569565 1101 22-23 0.2344129441161371 1101 24-25 0.2739378177772096 1101 26-27 0.2768832658011746 1101 28-29 0.2576069843941369 1101 30-31 0.03848711622044476 1101 32-33 0.1393457669263256 1101 34-35 0.050432848483609405 1101 36-37 0.30155251704835706 1101 38-39 0.17992826372879733 1101 40-41 0.3626344815523126 1101 42-43 0.20583126332918056 1101 44-45 -0.026334535583117713 1101 46-47 -0.09979802949401773 1101 48-49 0.08271816373162011 1101 50-51 0.07968568524894692 1101 52-53 -0.1510753606089068 1101 54-55 -0.17673354760064086 1101 56-57 -0.08710318993127686 1101 58-59 0.04290784043995899 1101 60-61 -0.1284928632680007 1101 62-63 -0.08475780155271906 1101 64-65 -0.17788891311647248 1101 66-67 -0.35026067902573743 1101 68-69 -8.687532283349856 1101 70-71 -6.579691206666606 1101 72-73 -1.6281639562587138 1101 74-75 -0.6854832232685943 1101 76-77 -0.5071792508350192 1101 78-79 -0.680231919763024 1101 80-81 -0.6007979605816196 1101 82-83 -0.43597723123454557 1101 84-85 -0.41659869815404704 1101 86-87 -0.4201258774316763 1101 88-89 -0.18225994589714745 1101 90-91 -0.449442499993733 1101 92-93 -0.7010990745648122 1101 94-95 -0.5189349810336843 1101 96-97 -0.7283368186171089 1101 98-99 -0.6825370417909156 1101 100-101 -0.6457432123781572 1102 1 0.2126421847092601 1102 2 0.13214152062625573 1102 3 0.28454981348541963 1102 4 0.13483175639100153 1102 5 0.29094467920810274 1102 6 0.24459521097482906 1102 7 0.3629968734555362 1102 8 0.19258915406351207 1102 9 0.3164709719271528 1102 10-11 0.27423366571809993 1102 12-13 0.2208852179327181 1102 14-15 0.3971898641025575 1102 16-17 0.39744182612417234 1102 18-19 0.3530669303886711 1102 20-21 0.27825699468331067 1102 22-23 0.26374208093337614 1102 24-25 0.07849483757541265 1102 26-27 0.14289471113592356 1102 28-29 0.20213208777857972 1102 30-31 0.08615566111776474 1102 32-33 0.044784993510468496 1102 34-35 0.006252528743630137 1102 36-37 0.09428204223704739 1102 38-39 0.3221998368998129 1102 40-41 0.1736950171679652 1102 42-43 0.039403774264791025 1102 44-45 0.07243746093625703 1102 46-47 -0.12116205895991783 1102 48-49 -0.14097278877019193 1102 50-51 -0.05752077012031975 1102 52-53 -0.07069822333953368 1102 54-55 -0.23132988974289503 1102 56-57 -0.30028214685211907 1102 58-59 -0.4198467017573222 1102 60-61 -0.25974364971487063 1102 62-63 -0.2474792596625619 1102 64-65 -0.16153921564526286 1102 66-67 0.1306297281351405 1102 68-69 -4.699213285565772 1102 70-71 -0.07623124027854189 1102 72-73 0.23876909818367054 1102 74-75 0.04778038732769474 1102 76-77 -0.007875056380559187 1102 78-79 -0.13951463222098326 1102 80-81 -0.2930991521419557 1102 82-83 -0.27279576834737185 1102 84-85 -0.35363376142169756 1102 86-87 -0.30062887286121764 1102 88-89 -0.37704177753460044 1102 90-91 -0.522687363829121 1102 92-93 -0.7831045567660517 1102 94-95 -0.3245782306002063 1102 96-97 -0.6840747883791565 1102 98-99 -0.5193591778979432 1102 100-101 -0.8135084609166974 1103 1 0.1575238410755091 1103 2 0.19915959769835467 1103 3 0.22449430980246277 1103 4 0.21501382922013335 1103 5 0.2699328353171069 1103 6 0.1887884967547464 1103 7 0.14481330373135393 1103 8 0.30597789580359347 1103 9 0.20691895006020644 1103 10-11 0.30606293181607214 1103 12-13 0.30619937004859565 1103 14-15 0.3569397693556624 1103 16-17 0.4201745964809902 1103 18-19 0.3654353698279422 1103 20-21 0.25619552459263417 1103 22-23 0.3044776873452477 1103 24-25 0.13320031765104545 1103 26-27 0.1829002636214625 1103 28-29 0.1526882361638613 1103 30-31 0.2674649573230212 1103 32-33 0.2706277115500626 1103 34-35 0.06569733841352843 1103 36-37 0.1900781649189227 1103 38-39 0.3570475061262357 1103 40-41 0.34433390764478844 1103 42-43 0.2026162814899024 1103 44-45 0.27034691572346503 1103 46-47 0.12396892994357955 1103 48-49 0.18634345518771056 1103 50-51 -0.03305542842809217 1103 52-53 0.04831088824160901 1103 54-55 0.0360025723424684 1103 56-57 0.20704128516619136 1103 58-59 0.032342507259620845 1103 60-61 -0.13096356625292316 1103 62-63 -0.32042060551307117 1103 64-65 -0.2467969960060863 1103 66-67 0.04684409748076135 1103 68-69 -4.5105552826820805 1103 70-71 0.2616262572178094 1103 72-73 0.6743881870890753 1103 74-75 0.2606499266778286 1103 76-77 0.12469490282004969 1103 78-79 -0.06623174763857875 1103 80-81 -0.057351678549341756 1103 82-83 -0.116972195532 1103 84-85 -0.279506623794159 1103 86-87 -0.21268231857812836 1103 88-89 -0.22327762637149107 1103 90-91 -0.3112681400549526 1103 92-93 -0.4502080383703344 1103 94-95 -0.4497157194581831 1103 96-97 -0.8249562732482403 1103 98-99 -0.35373525637166026 1103 100-101 -0.3008614709037616 1104 1 0.1794146388312967 1104 2 0.21282203411520584 1104 3 0.19940376802619753 1104 4 0.13569311605365186 1104 5 0.2613743043133141 1104 6 0.2616752148369059 1104 7 0.10159166342336334 1104 8 0.1483583485046367 1104 9 0.2277016011736066 1104 10-11 0.2717990062634499 1104 12-13 0.16946246586367408 1104 14-15 0.37199699615007376 1104 16-17 0.3458797557545168 1104 18-19 0.14914796693782506 1104 20-21 0.24918039952812876 1104 22-23 0.22023305436686513 1104 24-25 0.11393048668849559 1104 26-27 0.25502429762031653 1104 28-29 0.1821996007448874 1104 30-31 0.27107564885985624 1104 32-33 0.1637197935952699 1104 34-35 0.17411337101327717 1104 36-37 0.23614545992747793 1104 38-39 0.23544664595975462 1104 40-41 -0.02108744688214159 1104 42-43 0.04802747212406899 1104 44-45 0.10211527621540739 1104 46-47 0.07534929010299862 1104 48-49 0.22305110681087825 1104 50-51 0.1887656494150889 1104 52-53 0.10603097651689808 1104 54-55 0.0601367200420313 1104 56-57 0.15068030384862396 1104 58-59 0.090527264243768 1104 60-61 0.1659385639296076 1104 62-63 0.1938837403353375 1104 64-65 0.10818009602263245 1104 66-67 0.114814346135379 1104 68-69 -4.57804047210222 1104 70-71 0.48153062565264904 1104 72-73 0.8613979321918173 1104 74-75 0.3907340297535029 1104 76-77 0.28407313295379666 1104 78-79 -2.8808579585870575E-5 1104 80-81 0.031287112700297826 1104 82-83 0.036084579533834926 1104 84-85 0.07073516078464337 1104 86-87 0.08921862738541009 1104 88-89 0.09005181707695797 1104 90-91 -0.01885545463841609 1104 92-93 -0.3426762487566428 1104 94-95 0.014825156487429325 1104 96-97 -0.41818489338999854 1104 98-99 -0.22787748185636758 1104 100-101 -0.3123848627579022 1105 1 0.18776181619954713 1105 2 0.24494624284907118 1105 3 0.20165562586150187 1105 4 0.1319490474272058 1105 5 0.2501557715548799 1105 6 0.2603786348525716 1105 7 0.19364936820069545 1105 8 0.2817186073683686 1105 9 0.3359098911868088 1105 10-11 0.2556453971643009 1105 12-13 0.21479569822626843 1105 14-15 0.294344450136073 1105 16-17 0.3309101863368724 1105 18-19 0.28249073998346574 1105 20-21 0.39011030848092076 1105 22-23 0.3577481013734811 1105 24-25 0.2794826625290341 1105 26-27 0.29165103877393506 1105 28-29 0.4126145641876988 1105 30-31 0.5335276548907544 1105 32-33 0.4184217695190071 1105 34-35 0.21698057713715002 1105 36-37 0.25637352758418075 1105 38-39 0.6032873867063344 1105 40-41 0.45223274447844375 1105 42-43 0.44572505003586826 1105 44-45 0.4888999945044148 1105 46-47 0.5331642938289249 1105 48-49 0.5202918710121267 1105 50-51 0.5249019725340816 1105 52-53 0.43969233327965185 1105 54-55 0.6279688099101932 1105 56-57 0.49193799958066364 1105 58-59 0.5775976192873884 1105 60-61 0.5504841771980082 1105 62-63 0.6912089360581746 1105 64-65 0.6028800525315923 1105 66-67 1.2283460817119476 1105 68-69 2.407931168793244 1105 70-71 2.3998915760607638 1105 72-73 1.7337957663694716 1105 74-75 1.0783700049967386 1105 76-77 0.7536813349331375 1105 78-79 0.7177561353159163 1105 80-81 0.9651736289521189 1105 82-83 0.8592411667438924 1105 84-85 0.5763561282000182 1105 86-87 0.6192678085495267 1105 88-89 0.972381201507794 1105 90-91 1.1199900922101698 1105 92-93 0.9131415327163843 1105 94-95 1.2434923269560123 1105 96-97 0.8968574853964704 1105 98-99 1.317829657774066 1105 100-101 1.3116931876233409 1106 1 0.467270880293956 1106 2 0.37447127708071193 1106 3 0.388229878667385 1106 4 0.3086095945224514 1106 5 0.3404045446317312 1106 6 0.42143371932765206 1106 7 0.35889196152879066 1106 8 0.45939235551791313 1106 9 0.4557300813286531 1106 10-11 0.5797473894042042 1106 12-13 0.5274293266136709 1106 14-15 0.7729601586407995 1106 16-17 0.6287692158524649 1106 18-19 0.6503997907462846 1106 20-21 0.5956840252456743 1106 22-23 0.555712117182523 1106 24-25 0.6844981188444663 1106 26-27 0.6935037135636151 1106 28-29 0.6360558093584672 1106 30-31 0.7210172048661647 1106 32-33 0.9201965378131405 1106 34-35 0.9292542525184189 1106 36-37 0.9041007687499203 1106 38-39 0.9620236072553183 1106 40-41 0.8155541997328939 1106 42-43 0.8208278838325711 1106 44-45 0.8911499785342798 1106 46-47 0.9474179884854621 1106 48-49 0.9945063485664392 1106 50-51 1.0646449783083227 1106 52-53 0.9314073038251607 1106 54-55 1.0933609670452356 1106 56-57 0.9646898903060404 1106 58-59 1.0822629824205379 1106 60-61 1.146405404148929 1106 62-63 1.1621927242858234 1106 64-65 1.284133472224017 1106 66-67 1.8243179964897394 1106 68-69 3.315945955751612 1106 70-71 3.611738779342563 1106 72-73 2.474806106686149 1106 74-75 2.066538216369146 1106 76-77 1.4766065363651713 1106 78-79 1.3770024281610205 1106 80-81 1.490500322411581 1106 82-83 1.4476701774008376 1106 84-85 1.5710559868295846 1106 86-87 1.425175370617815 1106 88-89 1.610289863432488 1106 90-91 1.5614824529297415 1106 92-93 1.662946419300635 1106 94-95 2.146207528026981 1106 96-97 1.9722973585241448 1106 98-99 2.1373851602501333 1106 100-101 2.2468490145014997 1107 1 0.010172119192276341 1107 2 0.02471330421581186 1107 3 -0.052890882350112634 1107 4 -0.018449740709684193 1107 5 -0.007156242763215914 1107 6 -0.0323229627258641 1107 7 -0.050219483197821546 1107 8 -0.042143990644028406 1107 9 0.02222791360735954 1107 10-11 0.003851187476406892 1107 12-13 -0.1262172155551724 1107 14-15 -0.04709582328784734 1107 16-17 -0.15191631723860866 1107 18-19 -0.037343814826648725 1107 20-21 0.02872739370216948 1107 22-23 0.03970816056501292 1107 24-25 0.036265969222583294 1107 26-27 0.03625412395745542 1107 28-29 0.02145771470831903 1107 30-31 0.03940004268694253 1107 32-33 0.06972932021321299 1107 34-35 -0.008624747944587341 1107 36-37 -0.07272776379395651 1107 38-39 0.14415569093357306 1107 40-41 -0.11833240529285405 1107 42-43 -0.19655808280275977 1107 44-45 -0.18676732535359264 1107 46-47 -0.2536006300657476 1107 48-49 -0.1294445164174931 1107 50-51 -0.2553745241680616 1107 52-53 -0.20776601566468145 1107 54-55 -0.3197779495150357 1107 56-57 -0.26975583247818946 1107 58-59 -0.25778724697909894 1107 60-61 -0.17507397794527435 1107 62-63 0.055064499011173496 1107 64-65 -0.22105222221847853 1107 66-67 0.28669435219246253 1107 68-69 1.6280494170904056 1107 70-71 2.066771540696788 1107 72-73 1.0863104631745806 1107 74-75 0.12980060184471043 1107 76-77 -0.07707508220006076 1107 78-79 0.08133716922706213 1107 80-81 0.15481780589059824 1107 82-83 0.14433721821573187 1107 84-85 0.08072163861918114 1107 86-87 0.08295180769777488 1107 88-89 0.26148737160529123 1107 90-91 0.24718531376062813 1107 92-93 0.2015781592302055 1107 94-95 0.6018562309018449 1107 96-97 0.08063291336258516 1107 98-99 0.37907766652881136 1107 100-101 0.4242696366164651 1108 1 -0.2598838282991416 1108 2 -0.30393985289961734 1108 3 -0.13673664457121504 1108 4 -0.09072341547321372 1108 5 -0.1295587051664171 1108 6 -0.0888728811443471 1108 7 -0.14785231560647816 1108 8 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1.1876964877386236 2316 72-73 0.3400265438314882 2316 74-75 -0.08102603001619357 2316 76-77 -0.28633151780914545 2316 78-79 -0.6784176560602191 2316 80-81 -0.7627463609287481 2316 82-83 -0.5933908728526234 2316 84-85 -0.8461189508782212 2316 86-87 -0.7277678479189724 2316 88-89 -0.868761391694818 2316 90-91 -0.9110871372445537 2316 92-93 -0.899689808312008 2316 94-95 -0.6986859940526138 2316 96-97 -0.6638369523455125 2316 98-99 -0.9502529786446949 2316 100-101 -0.9761572841484352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 28.0 4 60.0 5 111.0 6 285.0 7 713.0 8 1755.0 9 2821.0 10 4459.0 11 6861.0 12 9488.0 13 12044.0 14 14629.0 15 17549.0 16 21484.0 17 24533.0 18 23571.0 19 21830.0 20 22127.0 21 23205.0 22 25489.0 23 28668.0 24 34237.0 25 41907.0 26 51916.0 27 63488.0 28 77932.0 29 94068.0 30 111294.0 31 131356.0 32 156652.0 33 187887.0 34 230341.0 35 284875.0 36 345182.0 37 350024.0 38 176265.0 39 11829.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.72043307336979 19.723402771871836 14.538612593023354 24.017551561735022 2 16.050436907894987 20.684074027252635 38.8059255513544 24.459563513497983 3 19.596379425707028 23.897774272903487 30.102103933140476 26.403742368249006 4 13.510189699613553 16.86583913258291 36.43347108545866 33.190500082344876 5 13.641864900784 37.46454382853882 34.559646414933916 14.333944855743269 6 32.938371563058936 36.458251148040766 16.714630960907247 13.888746327993045 7 28.728135520515362 32.28669804708595 20.56909884066075 18.416067591737935 8 26.440594874701738 35.934652638674514 19.18830932565292 18.43644316097083 9 25.95824540304944 16.358709598348504 19.792567513223055 37.890477485379 10-11 25.186386668556132 26.430790089506967 27.371283469361963 21.011539772574945 12-13 25.756423857799977 23.498565667165845 27.82293553736734 22.922074937666846 14-15 22.36523207849956 25.229703903147104 25.721609210370094 26.683454807983242 16-17 22.387259401281636 28.33729196592595 26.574703064255473 22.70074556853694 18-19 22.31143980972589 27.07053317349491 28.52455217792621 22.093474838852995 20-21 22.766558022497385 26.300436236341284 28.719230783961514 22.213774957199814 22-23 22.440031865551884 26.213610267448495 28.65027173808968 22.696086128909947 24-25 22.47767877660699 26.415605246789696 28.665747722683694 22.440968253919618 26-27 22.23759969022924 26.707540474529896 28.48374335446347 22.5711164807774 28-29 22.20428293425386 27.038373139147296 28.39480544947523 22.362538477123607 30-31 22.368371161707252 26.71468605979642 28.29213327363624 22.624809504860092 32-33 22.19239872282396 26.5948949344033 28.37959390888219 22.833112433890552 34-35 22.695506079100408 26.785366495440314 28.17654947782761 22.34257794763166 36-37 22.818934754708337 26.43546638627674 28.286576651787726 22.459022207227196 38-39 22.331679494080067 26.79599122246093 28.265872486463362 22.60645679699564 40-41 22.648615707378774 26.571693500477622 28.370814787181654 22.408876004961957 42-43 22.388203049651683 26.598693849914472 28.23974929831863 22.773353802115214 44-45 22.44714484496786 26.744086412305577 28.307701508810933 22.50106723391563 46-47 22.698389423192115 26.863309421467008 27.82382848626865 22.61447266907223 48-49 22.53354418248035 26.96005367069197 27.77864673183822 22.727755414989456 50-51 22.43772330670265 27.12626316838667 27.808297425359974 22.627716099550703 52-53 22.720921537359747 26.906114395322554 27.710780801228346 22.66218326608935 54-55 22.333379577927378 26.9258318469912 27.897485699306408 22.843302875775013 56-57 22.384520044366372 27.0900372976359 27.68712513194015 22.83831752605758 58-59 22.37257666697694 26.855764306336184 27.696966493258852 23.074692533428024 60-61 22.203491789348128 26.998291087397387 27.939696432925036 22.85852069032945 62-63 22.231731432495607 26.94995516904201 27.985376030432196 22.832937368030183 64-65 21.934889325488836 26.954918385196443 27.92007067480226 23.190121614512467 66-67 21.795561342377244 27.020522969943862 27.92327738368482 23.26063830399407 68-69 21.974721369202108 26.5208858176318 28.39645297503026 23.107939838135827 70-71 21.853243541569714 26.566445776876957 28.13473764369695 23.44557303785638 72-73 21.81571402340083 26.779923585924198 27.9298186235374 23.474543767137572 74-75 21.926484419746416 27.04343928327194 27.406937029608898 23.623139267372746 76-77 21.91771191296307 26.85077892296131 27.544887467407992 23.686621696667633 78-79 22.064860871112284 26.940236924247785 27.393693472336146 23.601208732303792 80-81 22.06664925263949 26.923401005468566 27.26598113616569 23.743968605726256 82-83 22.026683957817202 26.892481892262627 27.357041129741273 23.723793020178903 84-85 21.96999916314322 26.893321442434694 27.220729689323143 23.915949705098946 86-87 22.014633832464682 26.840182984186512 27.186399819834726 23.95878336351408 88-89 21.92616523009692 26.871214282841784 27.048543354660058 24.154077132401238 90-91 21.852678780882623 26.802496941252713 27.13877242977616 24.206051848088507 92-93 21.85805305057059 26.5411418646025 27.291762817873494 24.309042266953412 94-95 21.561342493657012 26.317480116237927 27.58040422617868 24.540773163926378 96-97 21.81453119681963 26.08368963282908 27.48017680039701 24.621602369954275 98-99 21.62693544497685 26.275355265327793 27.20957096562948 24.888138324065874 100-101 21.480725048588614 26.112865008308134 27.498473585950713 24.907936357152543 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 359.0 1 265.0 2 188.5 3 228.0 4 231.0 5 240.5 6 407.0 7 826.5 8 1268.5 9 1481.0 10 1433.0 11 1334.0 12 1413.0 13 1532.0 14 1662.5 15 1881.5 16 2135.0 17 2429.5 18 2706.5 19 3077.0 20 3616.5 21 4317.5 22 5373.0 23 6716.5 24 8327.5 25 10583.5 26 13832.5 27 17802.0 28 21195.5 29 24439.5 30 28612.0 31 34471.0 32 41827.5 33 46376.5 34 49157.5 35 53901.5 36 59713.5 37 65029.0 38 68682.0 39 71382.5 40 74964.5 41 79687.5 42 83138.5 43 85631.0 44 89327.5 45 92856.5 46 95066.0 47 97893.0 48 100563.5 49 99805.5 50 97442.5 51 96189.5 52 95075.5 53 94465.5 54 93395.5 55 87588.0 56 79788.5 57 72826.5 58 65928.5 59 57547.5 60 47817.5 61 39827.5 62 32506.5 63 24615.0 64 17859.0 65 13109.0 66 9634.0 67 7399.0 68 5617.0 69 3979.0 70 2799.0 71 1880.5 72 1302.5 73 1012.5 74 758.0 75 535.0 76 333.5 77 198.5 78 130.0 79 73.0 80 44.5 81 37.0 82 23.5 83 9.0 84 5.0 85 4.0 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023133165068920748 2 0.02221396645691065 3 1.1489982650126199E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.638494505873296E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.659988433417466E-5 26-27 2.680995951696113E-4 28-29 1.1489982650126199E-4 30-31 4.2129936383796066E-4 32-33 0.002049046905939172 34-35 0.0037725443034581016 36-37 0.10331409399571807 38-39 0.0955200557647158 40-41 0.10434819243422944 42-43 0.10610998977391543 44-45 0.0992543001260068 46-47 0.09316460932143993 48-49 0.0946008571527057 50-51 0.09383485830936396 52-53 0.07424443789089878 54-55 0.06342470422869662 56-57 0.033895448817872285 58-59 0.013826279122318525 60-61 0.00940263580201994 62-63 0.001953297050521454 64-65 0.005304541990141595 66-67 0.16396205241730083 68-69 0.435968241687955 70-71 0.41670337077791014 72-73 0.19289765872453532 74-75 0.02968245517949268 76-77 0.03291880029261156 78-79 0.005189642163640333 80-81 0.0018383972240201918 82-83 1.9149971083543665E-5 84-85 3.638494505873296E-4 86-87 0.0013021980336809691 88-89 1.9149971083543663E-4 90-91 5.936491035898535E-4 92-93 0.0024894962408606764 94-95 0.08360877375075164 96-97 0.017445623657108278 98-99 0.018824421575123422 100-101 0.010570784038116102 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2610970.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.59928790502485 #Duplication Level Percentage of deduplicated Percentage of total 1 73.94315564442596 40.37243643636076 2 14.384338610894886 15.707492902792303 3 5.003301907236397 8.195301639268799 4 2.286762567228253 4.994224311141165 5 1.235216204743069 3.3720962593859474 6 0.7400531978895363 2.4243826569963067 7 0.485703210138295 1.8563334584714886 8 0.3500063819799876 1.5288079374657157 9 0.2445212455260027 1.2015617295032541 >10 1.2126629853649091 11.857347361556156 >50 0.07720304833373029 2.8915322132621983 >100 0.03356582321469648 3.3175813970715806 >500 0.0023862376565327824 0.9492548566330753 >1k 9.825684468029287E-4 0.9006655155104496 >5k 1.4036692097184697E-4 0.43098132458075333 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6142 0.23523824479025038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5105 0.19552120476298082 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3540 0.13558179527148914 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.829994216708733E-5 0.0 10-11 0.0 0.0 0.0 3.829994216708733E-5 0.0 12-13 0.0 0.0 0.0 7.659988433417466E-5 0.0 14-15 3.829994216708733E-5 0.0 0.0 1.5319976866834932E-4 0.0 16-17 3.829994216708733E-5 0.0 0.0 1.7234973975189296E-4 3.829994216708733E-5 18-19 3.829994216708733E-5 0.0 0.0 2.680995951696113E-4 3.829994216708733E-5 20-21 3.829994216708733E-5 0.0 0.0 3.255495084202423E-4 3.829994216708733E-5 22-23 3.829994216708733E-5 0.0 0.0 5.361991903392226E-4 3.829994216708733E-5 24-25 3.829994216708733E-5 0.0 0.0 9.383485830936395E-4 3.829994216708733E-5 26-27 3.829994216708733E-5 0.0 0.0 0.0016468975131847551 5.7449913250630994E-5 28-29 3.829994216708733E-5 0.0 0.0 0.004921542568470722 7.659988433417466E-5 30-31 3.829994216708733E-5 0.0 0.0 0.016871124524601967 7.659988433417466E-5 32-33 3.829994216708733E-5 0.0 0.0 0.04707062892335033 7.659988433417466E-5 34-35 3.829994216708733E-5 0.0 0.0 0.09630520457914109 7.659988433417466E-5 36-37 3.829994216708733E-5 0.0 0.0 0.18152257590091037 7.659988433417466E-5 38-39 3.829994216708733E-5 0.0 0.0 0.31333182686894145 7.659988433417466E-5 40-41 3.829994216708733E-5 0.0 0.0 0.4571289597352708 7.659988433417466E-5 42-43 3.829994216708733E-5 0.0 0.0 0.6049667365002279 7.659988433417466E-5 44-45 3.829994216708733E-5 0.0 0.0 0.7800166221749005 7.659988433417466E-5 46-47 3.829994216708733E-5 0.0 0.0 0.9972155942044527 7.659988433417466E-5 48-49 3.829994216708733E-5 0.0 0.0 1.2307111916261007 7.659988433417466E-5 50-51 3.829994216708733E-5 0.0 0.0 1.4774011191243102 7.659988433417466E-5 52-53 3.829994216708733E-5 0.0 0.0 1.7140373118036591 7.659988433417466E-5 54-55 3.829994216708733E-5 0.0 0.0 1.9644614836631598 7.659988433417466E-5 56-57 3.829994216708733E-5 0.0 0.0 2.2507727013332213 7.659988433417466E-5 58-59 3.829994216708733E-5 0.0 0.0 2.5471951037353935 7.659988433417466E-5 60-61 3.829994216708733E-5 0.0 0.0 2.8483092490530337 1.3404979758480565E-4 62-63 3.829994216708733E-5 0.0 0.0 3.138297261171136 1.9149971083543663E-4 64-65 3.829994216708733E-5 0.0 0.0 3.441900902729637 1.9149971083543663E-4 66-67 3.829994216708733E-5 0.0 0.0 3.7703803567256613 1.9149971083543663E-4 68-69 3.829994216708733E-5 0.0 0.0 4.135646905173173 1.9149971083543663E-4 70-71 3.829994216708733E-5 0.0 0.0 4.551297027541488 2.2979965300252397E-4 72-73 3.829994216708733E-5 0.0 0.0 4.979509530940608 2.2979965300252397E-4 74-75 3.829994216708733E-5 0.0 0.0 5.405098488301283 2.2979965300252397E-4 76-77 3.829994216708733E-5 0.0 0.0 5.869217187482047 2.2979965300252397E-4 78-79 3.829994216708733E-5 0.0 0.0 6.38213001298368 2.2979965300252397E-4 80-81 3.829994216708733E-5 0.0 0.0 6.930240485336867 2.2979965300252397E-4 82-83 3.829994216708733E-5 0.0 0.0 7.481587302803172 2.680995951696113E-4 84-85 3.829994216708733E-5 0.0 0.0 8.017556693489393 2.680995951696113E-4 86-87 3.829994216708733E-5 0.0 0.0 8.558505076657333 2.680995951696113E-4 88-89 3.829994216708733E-5 0.0 0.0 9.134727706561163 2.680995951696113E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4770 0.0 39.697536 1 GTATCAA 7485 0.0 33.604164 1 ATCAACG 9295 0.0 26.537703 3 TCAACGC 9320 0.0 26.466518 4 TATCAAC 9505 0.0 26.204435 2 CAACGCA 9570 0.0 25.725557 5 AACGCAG 9915 0.0 24.830418 6 TACATGG 8130 0.0 23.750427 2 GTACATG 8225 0.0 23.541456 1 ACATGGG 8165 0.0 22.193026 3 ACGCAGA 11565 0.0 21.205782 7 CGCAGAG 11640 0.0 21.028395 8 TAAGGTG 1350 0.0 19.325836 5 GTGTAGC 1670 0.0 19.3242 1 GAGTACT 6880 0.0 18.995869 12-13 AGAGTAC 10875 0.0 18.80034 10-11 GCAGAGT 12875 0.0 18.679708 9 AGTACTT 7435 0.0 18.279724 12-13 GTACTTT 7205 0.0 17.842731 14-15 GTAAGGT 1250 0.0 17.83599 4 >>END_MODULE