FastQCFastQC Report
Fri 27 May 2016
SRR939815_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939815_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678723
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79260.2958872567264327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58430.21812632362510045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36400.13588564401769052No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26840.10019699685260477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83500.031.1921981
GTATCAA123500.029.5021971
TACATGG109550.027.2832682
GTACATG114250.025.662111
ACATGGG112350.025.3374333
TATCAAC148000.025.3240762
TCAACGC149050.025.0829544
CAACGCA150400.024.7947185
ATCAACG151050.024.782253
AACGCAG154200.024.3067656
TACCTGG18900.022.3406832
ACGCAGA170850.021.771367
CATGGGA61600.021.6426334
CGCAGAG172600.021.4948398
GAGTACT82800.021.40032412-13
GTACTTT86900.020.03747214-15
AGAGTAC133950.019.8865310-11
GCAGAGT179350.019.4686819
AGTACTT86200.019.45549212-13
CATGGGG54750.019.3243644
Produced by FastQC (version 0.11.5)