FastQCFastQC Report
Fri 27 May 2016
SRR939815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678723
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86570.32317637919262276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74550.27830425169007766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49370.18430423750421376No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33880.12647817635492734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84250.030.2837331
GTATCAA121350.029.0310571
TACATGG110500.024.4943982
GTACATG113000.024.2899481
TATCAAC148750.023.553152
TCAACGC149050.023.536064
ATCAACG150100.023.3718553
CAACGCA149700.023.3393755
AACGCAG153350.022.8304276
ACATGGG111950.022.7020783
CATGGGA58000.020.8928644
ACGCAGA172350.020.1768847
GAGTACT86450.020.08657312-13
CGCAGAG171950.020.0597748
AGAGTAC141650.019.39407510-11
GTACTTT89400.019.26616914-15
AGTACTT90100.018.27913912-13
GCAGAGT183600.018.2736139
GAGTACA88600.017.6188011
GTATAGC12650.017.1494161
Produced by FastQC (version 0.11.5)