##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939815_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2678723 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42625870610735 31.0 31.0 34.0 30.0 34.0 2 31.643157579189786 31.0 31.0 34.0 30.0 34.0 3 31.704139621752603 31.0 31.0 34.0 30.0 34.0 4 35.50723945700992 37.0 35.0 37.0 33.0 37.0 5 35.24090508798409 37.0 35.0 37.0 33.0 37.0 6 35.26708174006794 37.0 35.0 37.0 32.0 37.0 7 35.18861338033085 37.0 35.0 37.0 32.0 37.0 8 35.125847652034196 37.0 35.0 37.0 32.0 37.0 9 36.732789467220016 39.0 35.0 39.0 32.0 39.0 10-11 36.5871008312543 38.5 35.0 39.0 32.0 39.0 12-13 36.5280962234617 38.0 35.0 39.0 32.0 39.0 14-15 37.573994212914144 39.0 36.0 41.0 32.0 41.0 16-17 37.50677020356341 39.0 36.0 41.0 32.0 41.0 18-19 37.484983329743315 39.0 36.0 41.0 32.0 41.0 20-21 37.41622388727763 39.0 36.0 41.0 32.0 41.0 22-23 37.27445670940967 39.0 36.0 40.5 31.0 41.0 24-25 37.117110839754616 39.0 36.0 40.0 31.0 41.0 26-27 36.82998111413535 39.0 36.0 40.0 30.5 41.0 28-29 36.60723841173574 38.5 35.0 40.0 30.0 41.0 30-31 36.36431725116782 38.0 35.0 40.0 30.0 41.0 32-33 36.32606880218671 38.0 35.0 40.0 30.0 41.0 34-35 36.396258030412255 38.0 35.0 40.0 30.0 41.0 36-37 36.36358313270913 38.0 35.0 40.0 30.0 41.0 38-39 36.22833025288543 38.0 35.0 40.0 30.0 41.0 40-41 36.06486971590567 38.0 35.0 40.0 29.5 41.0 42-43 35.8615209560675 38.0 35.0 40.0 29.0 41.0 44-45 35.60518052818451 38.0 34.0 40.0 28.0 41.0 46-47 35.293942673430585 38.0 34.0 40.0 27.0 41.0 48-49 34.97797401971014 38.0 34.0 40.0 26.0 41.0 50-51 34.712137089202585 38.0 33.0 40.0 26.0 41.0 52-53 34.425444512179865 38.0 33.0 40.0 24.0 41.0 54-55 34.124236063228636 37.5 33.0 40.0 24.0 41.0 56-57 32.73166579747141 36.5 31.5 39.5 16.0 41.0 58-59 29.21218524647752 34.5 23.5 38.5 7.0 40.0 60-61 29.205586579874065 34.0 24.0 38.0 8.5 40.0 62-63 31.207409090077626 35.0 29.0 38.5 8.5 40.0 64-65 30.782282266587472 35.0 29.0 38.0 4.5 40.0 66-67 25.271680013200317 28.5 18.0 36.0 2.0 39.0 68-69 17.562817058725372 13.0 7.0 29.0 2.0 35.5 70-71 16.40101272136014 13.5 7.5 26.0 2.0 33.5 72-73 18.507178980432094 18.5 7.0 30.0 2.0 34.5 74-75 19.222720676979293 18.0 7.5 30.5 2.0 35.0 76-77 20.181174201289195 23.0 8.5 31.0 2.0 34.5 78-79 23.22793603519289 28.0 12.0 34.0 2.0 36.0 80-81 25.914353593111343 31.5 22.0 34.5 2.0 36.0 82-83 26.980613337026632 33.0 26.0 35.0 2.0 36.0 84-85 26.99248578520437 33.0 26.0 35.0 2.0 36.0 86-87 26.710202025368055 33.0 25.5 35.0 2.0 35.5 88-89 26.273995855487854 33.0 24.5 35.0 2.0 35.0 90-91 24.573772838774296 31.5 11.5 34.5 2.0 35.0 92-93 22.376654099733344 30.5 2.0 34.0 2.0 35.0 94-95 17.930915029288208 21.0 2.0 33.0 2.0 35.0 96-97 16.604737219936514 13.5 2.0 32.5 2.0 34.5 98-99 15.508474933765083 2.0 2.0 32.0 2.0 34.0 100-101 14.890770154286201 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38155534577670025 1101 2 0.46971088487437385 1101 3 0.4327752108523448 1101 4 0.3666714982935062 1101 5 0.3823882681892101 1101 6 0.33158897071026416 1101 7 0.4140574473910732 1101 8 0.5235029211682729 1101 9 0.5509647355175034 1101 10-11 0.5738404558931407 1101 12-13 0.6561168502405863 1101 14-15 0.7339935749697162 1101 16-17 0.5667817963443795 1101 18-19 0.613242589316684 1101 20-21 0.7049776825627774 1101 22-23 0.6093642355937305 1101 24-25 0.634543136635628 1101 26-27 0.560361568294752 1101 28-29 0.5028101129518134 1101 30-31 0.6727164196874327 1101 32-33 0.5813885370300369 1101 34-35 0.7526130376131306 1101 36-37 0.6836910515639971 1101 38-39 0.44687137107824526 1101 40-41 0.5185705941004315 1101 42-43 0.6611000196293588 1101 44-45 0.5365631683558192 1101 46-47 0.5360001742630232 1101 48-49 0.4466052954260391 1101 50-51 0.4002498493077624 1101 52-53 0.594527016271492 1101 54-55 0.6501585154903466 1101 56-57 1.3244835514878588 1101 58-59 -8.210836114970071 1101 60-61 -9.631412938953321 1101 62-63 -2.381766968423161 1101 64-65 0.269335250812869 1101 66-67 -0.8522331448151448 1101 68-69 -3.762798598326029 1101 70-71 -4.339712701655962 1101 72-73 -6.465137305307671 1101 74-75 -7.449663863277188 1101 76-77 -8.711480791055104 1101 78-79 -11.510771835637629 1101 80-81 -7.5280811653234885 1101 82-83 -3.620905865119088 1101 84-85 -2.697520531838819 1101 86-87 -2.5732701631355823 1101 88-89 -2.7330272021052338 1101 90-91 -8.360001568697928 1101 92-93 -14.179337046141358 1101 94-95 -12.22804700748933 1101 96-97 -12.29252979345544 1101 98-99 -11.976271472528044 1101 100-101 -11.843967815181205 1102 1 0.5219742324596126 1102 2 0.5239194333978148 1102 3 0.5576167355706154 1102 4 0.40345303578875047 1102 5 0.46620561346205847 1102 6 0.4910704925031695 1102 7 0.49286768359291955 1102 8 0.5904404710447011 1102 9 0.6908695320432017 1102 10-11 0.7136019954963686 1102 12-13 0.6796511017661899 1102 14-15 0.8179138458242434 1102 16-17 0.8670899277879798 1102 18-19 0.7438839759404416 1102 20-21 0.8453612927662917 1102 22-23 0.8745480712225415 1102 24-25 0.8375973484106609 1102 26-27 0.903415651009496 1102 28-29 0.8396927476746257 1102 30-31 0.8837804357425298 1102 32-33 0.6816383762424252 1102 34-35 0.8116038076513519 1102 36-37 0.803905227116104 1102 38-39 0.8416178644026004 1102 40-41 1.0103107355074812 1102 42-43 0.9430648119821043 1102 44-45 0.8400746837602426 1102 46-47 0.6451382881329195 1102 48-49 0.8565408943410802 1102 50-51 0.799097878664675 1102 52-53 0.7354719311884921 1102 54-55 0.5314610134522653 1102 56-57 1.2711407824132834 1102 58-59 -8.930612561036853 1102 60-61 -9.693772505224704 1102 62-63 -2.688399054101989 1102 64-65 0.11030630043870104 1102 66-67 -1.0505390134016572 1102 68-69 -3.6261204166134426 1102 70-71 -4.21388429591622 1102 72-73 -6.597146946111373 1102 74-75 -7.5621504463844875 1102 76-77 -8.603125105196455 1102 78-79 -11.723097308482266 1102 80-81 -7.555893550379167 1102 82-83 -3.436443863695125 1102 84-85 -2.4401706558442484 1102 86-87 -2.2363318173873665 1102 88-89 -2.3595187626377374 1102 90-91 -8.169058374584797 1102 92-93 -14.15314339977946 1102 94-95 -12.403658376840522 1102 96-97 -12.262151474723844 1102 98-99 -11.775455273740782 1102 100-101 -11.561182952232478 1103 1 0.26193092769787896 1103 2 0.32312491628169937 1103 3 0.3338366951289835 1103 4 0.1716383515377231 1103 5 0.21842900877010862 1103 6 0.29793483407394916 1103 7 0.4097296988748482 1103 8 0.29937699914805904 1103 9 0.3485203381917614 1103 10-11 0.3247359718398073 1103 12-13 0.2680813936259696 1103 14-15 0.42338867716830464 1103 16-17 0.3252883065435981 1103 18-19 0.39984822267022224 1103 20-21 0.37108467912707965 1103 22-23 0.41022081606444516 1103 24-25 0.38258866956378057 1103 26-27 0.2515000416112869 1103 28-29 0.35433047292201536 1103 30-31 0.3333625984753823 1103 32-33 0.3065600876752441 1103 34-35 0.39921542477999594 1103 36-37 0.1423981498296314 1103 38-39 0.23404489945799156 1103 40-41 0.3255228751563948 1103 42-43 0.3156289191345465 1103 44-45 0.22560785127092942 1103 46-47 0.2454550213075848 1103 48-49 0.14583728143553998 1103 50-51 0.28409331555961614 1103 52-53 0.2137569194675777 1103 54-55 0.2865922698271959 1103 56-57 0.8974537935257842 1103 58-59 -6.792292320534376 1103 60-61 -9.027572587539542 1103 62-63 -2.796025702635532 1103 64-65 -0.3881771381741146 1103 66-67 -1.8003108581487197 1103 68-69 -4.535676990168906 1103 70-71 -5.124612961163255 1103 72-73 -7.735203242301626 1103 74-75 -8.412230077041396 1103 76-77 -9.003000738215441 1103 78-79 -12.34588957088765 1103 80-81 -8.744331894777286 1103 82-83 -4.935826502422469 1103 84-85 -3.997625516893226 1103 86-87 -3.7755655736280858 1103 88-89 -3.9052258649765506 1103 90-91 -9.330077825938519 1103 92-93 -14.700237328165553 1103 94-95 -12.391311151387342 1103 96-97 -12.509905711253737 1103 98-99 -12.183356603304333 1103 100-101 -11.844484671896776 1104 1 0.2529244896538039 1104 2 0.21422009258867902 1104 3 0.22221404459718386 1104 4 0.2361161220098964 1104 5 0.19039963423526984 1104 6 0.14569474279559103 1104 7 0.232196838190184 1104 8 0.2984124298135882 1104 9 0.32947121788851774 1104 10-11 0.31391637090226254 1104 12-13 0.16859414672953932 1104 14-15 0.29336079008560034 1104 16-17 0.31495694113976924 1104 18-19 0.2596862744676187 1104 20-21 0.2020418535181463 1104 22-23 0.3306496645955903 1104 24-25 0.3186694870911708 1104 26-27 0.33729646061788543 1104 28-29 0.3112112879103748 1104 30-31 0.4435660334742053 1104 32-33 0.33782646908822755 1104 34-35 0.37673278676353306 1104 36-37 0.3546678050126246 1104 38-39 0.30164634586237327 1104 40-41 0.43792495695462463 1104 42-43 0.43790758789662476 1104 44-45 0.30215847515977146 1104 46-47 0.3525357060645362 1104 48-49 0.4261282497129315 1104 50-51 0.2778627583627937 1104 52-53 0.4112614170002118 1104 54-55 0.47689767358422586 1104 56-57 0.9031220599616887 1104 58-59 -6.616799416876525 1104 60-61 -8.618784162794729 1104 62-63 -2.4598404936860625 1104 64-65 -0.13972568587176326 1104 66-67 -1.6745535773297107 1104 68-69 -4.484819298396284 1104 70-71 -5.004027115550418 1104 72-73 -7.905089768534896 1104 74-75 -8.76898438983269 1104 76-77 -9.181245983737613 1104 78-79 -12.118376705114336 1104 80-81 -8.553588110523435 1104 82-83 -4.695959108886111 1104 84-85 -3.7425166319057546 1104 86-87 -3.585490137799848 1104 88-89 -3.794061431163147 1104 90-91 -9.38762720093885 1104 92-93 -14.864427685011243 1104 94-95 -12.736307213236152 1104 96-97 -12.289846768582533 1104 98-99 -11.735086427618887 1104 100-101 -11.386186757239077 1105 1 0.1676866056340991 1105 2 0.23027549047552753 1105 3 0.15225651757623737 1105 4 0.10707247341591142 1105 5 0.13358668464430679 1105 6 0.0997932824276333 1105 7 0.1504346394052476 1105 8 0.17076960367538163 1105 9 0.21398959814535345 1105 10-11 0.15706262552178174 1105 12-13 0.2612856350981261 1105 14-15 0.26878020613883535 1105 16-17 0.2514551434352015 1105 18-19 0.24026463060035752 1105 20-21 0.2803492000829877 1105 22-23 0.16526651381044388 1105 24-25 0.18170401873599218 1105 26-27 0.30203645140915825 1105 28-29 0.3494162678974888 1105 30-31 0.2159951471639161 1105 32-33 0.3234299329592929 1105 34-35 0.4543625329330254 1105 36-37 0.20758566650103205 1105 38-39 0.2378870362737473 1105 40-41 0.14689591678698832 1105 42-43 0.04143975960919022 1105 44-45 0.19332008186515282 1105 46-47 0.23466751944924624 1105 48-49 0.2662973610503627 1105 50-51 0.3173301135562028 1105 52-53 0.40443464905521154 1105 54-55 0.18475169374952571 1105 56-57 0.7247046939825736 1105 58-59 -6.680562418391794 1105 60-61 -8.798513876844464 1105 62-63 -2.7344506130460537 1105 64-65 -0.3628964548842646 1105 66-67 -1.6417915520653281 1105 68-69 -4.05816667335732 1105 70-71 -4.550789190392102 1105 72-73 -6.171484955528346 1105 74-75 -6.581587335251774 1105 76-77 -8.32773569987592 1105 78-79 -11.866517424480971 1105 80-81 -7.950981293855545 1105 82-83 -4.004413911001482 1105 84-85 -3.168091741780131 1105 86-87 -2.883510632212115 1105 88-89 -3.014048704564555 1105 90-91 -8.60614719720473 1105 92-93 -12.005526528963525 1105 94-95 -12.028169178427614 1105 96-97 -12.166151535018068 1105 98-99 -11.510654733799857 1105 100-101 -11.16152207647734 1106 1 0.05704855642785489 1106 2 0.1091476814675616 1106 3 0.06444678049506791 1106 4 0.0517881622579921 1106 5 -0.05400946422731323 1106 6 -0.0420320497022999 1106 7 0.049948731589687156 1106 8 0.08488277701234637 1106 9 0.11425773605450473 1106 10-11 0.1036725426303704 1106 12-13 0.13562725239084017 1106 14-15 0.09613516492097318 1106 16-17 0.03504556177376372 1106 18-19 0.08389436493126112 1106 20-21 -0.04462219148118862 1106 22-23 0.03640645241991791 1106 24-25 0.11506137448822784 1106 26-27 0.05717518054322568 1106 28-29 0.047450374460531464 1106 30-31 0.031161799846920246 1106 32-33 0.10574781536944045 1106 34-35 0.0487123367015414 1106 36-37 0.10095293233873548 1106 38-39 0.1942873511876897 1106 40-41 0.18618161862718097 1106 42-43 0.18990065390796218 1106 44-45 0.09202758905646391 1106 46-47 -0.039698839549970444 1106 48-49 0.05351928826041785 1106 50-51 0.07539087039508985 1106 52-53 -0.0958602141858762 1106 54-55 -0.36320144604807325 1106 56-57 0.39821468971241814 1106 58-59 -6.949794672248672 1106 60-61 -9.086259856451182 1106 62-63 -2.8964728039174616 1106 64-65 -0.3994288712107874 1106 66-67 -1.8126241895660868 1106 68-69 -4.382780342198558 1106 70-71 -4.91082116160916 1106 72-73 -6.344670199606753 1106 74-75 -6.720633715363196 1106 76-77 -8.603288213276784 1106 78-79 -12.147313611523984 1106 80-81 -8.3488545391177 1106 82-83 -4.417301123399245 1106 84-85 -3.534620019298977 1106 86-87 -3.4513793044134573 1106 88-89 -3.645449615906415 1106 90-91 -8.886592161918983 1106 92-93 -11.892042111279558 1106 94-95 -11.889250393682337 1106 96-97 -12.247929174359044 1106 98-99 -11.795439750351067 1106 100-101 -11.606890351283447 1107 1 0.04097292775305661 1107 2 0.13044262840277554 1107 3 0.08092857763265116 1107 4 -0.010535819702027993 1107 5 0.015277042537945817 1107 6 0.069505342078088 1107 7 0.03660698056212652 1107 8 0.09204932253752673 1107 9 0.04355084372620155 1107 10-11 0.03847976177979007 1107 12-13 0.05607268380502717 1107 14-15 0.016552501678638976 1107 16-17 0.09449338671890217 1107 18-19 0.005593018702207075 1107 20-21 0.09628455151732851 1107 22-23 0.16276892619990946 1107 24-25 0.2015535171486107 1107 26-27 0.2734590173796292 1107 28-29 0.3496225195183271 1107 30-31 0.23805071665209 1107 32-33 0.21337316001395124 1107 34-35 0.20884204571715514 1107 36-37 0.1657823556316913 1107 38-39 0.37077624423280753 1107 40-41 0.09497525949532104 1107 42-43 0.21206780614394205 1107 44-45 0.19365860427293313 1107 46-47 0.13015703887562324 1107 48-49 0.1071777409860104 1107 50-51 0.31121554421947195 1107 52-53 0.32112127986387407 1107 54-55 0.2308868228140355 1107 56-57 0.9463152166745274 1107 58-59 -6.563645725885252 1107 60-61 -8.515104319215126 1107 62-63 -2.3607161994323 1107 64-65 -0.04646820948262942 1107 66-67 -1.7318966021786615 1107 68-69 -3.779279962051099 1107 70-71 -4.53854850055955 1107 72-73 -6.168884542571069 1107 74-75 -6.8667433880724875 1107 76-77 -9.166004115695213 1107 78-79 -12.408396652505658 1107 80-81 -8.319073266721407 1107 82-83 -4.24582712654237 1107 84-85 -3.279718003427103 1107 86-87 -3.022717055564634 1107 88-89 -3.1531165807777946 1107 90-91 -8.414896993223278 1107 92-93 -11.709505709635138 1107 94-95 -12.097029844207045 1107 96-97 -12.521145628569904 1107 98-99 -11.756117198141496 1107 100-101 -11.332602754846866 1108 1 -0.08195281126138099 1108 2 -0.08069171149425358 1108 3 -0.06291686040108502 1108 4 0.025495743352635714 1108 5 -0.17640028395777563 1108 6 -0.053045722621426705 1108 7 0.04747943154127654 1108 8 -0.0038968009816358062 1108 9 -0.09198931365999385 1108 10-11 -0.07534786581539521 1108 12-13 -0.05786680974103575 1108 14-15 -0.025130973613350704 1108 16-17 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1.0033838072423293 2311 58-59 4.479946887194004 2311 60-61 4.100398983139929 2311 62-63 1.563314676274853 2311 64-65 1.3278341687747037 2311 66-67 -0.3122075693675761 2311 68-69 -3.2393773903779675 2311 70-71 -0.8557071930980236 2311 72-73 -0.8999887709775507 2311 74-75 1.5375189028058323 2311 76-77 4.72768899056992 2311 78-79 5.4938809284780525 2311 80-81 3.156062057182904 2311 82-83 1.9124700939601915 2311 84-85 1.545082261786991 2311 86-87 1.449177210462686 2311 88-89 1.658916531910112 2311 90-91 3.147671065573171 2311 92-93 5.08511308173588 2311 94-95 9.059991552416236 2311 96-97 9.769923482169194 2311 98-99 10.175843420251809 2311 100-101 9.277171275906738 2312 1 -0.24721207625783848 2312 2 -0.19035374765204693 2312 3 -0.31640300441758384 2312 4 -0.2541734827727282 2312 5 -0.28486128077091877 2312 6 -0.27272911648417164 2312 7 -0.2795169692309827 2312 8 -0.2479199857093377 2312 9 -0.34117031870641057 2312 10-11 -0.2650837892346303 2312 12-13 -0.3715244590637141 2312 14-15 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5.613409849169763 2312 80-81 3.1624321279614698 2312 82-83 1.8480110444151983 2312 84-85 1.6040060646440608 2312 86-87 1.2183950165864559 2312 88-89 1.4135002865444228 2312 90-91 2.7495921980302 2312 92-93 4.860104655716331 2312 94-95 8.810726560582996 2312 96-97 9.391912800569024 2312 98-99 9.882776089874064 2312 100-101 9.228840924088011 2313 1 -0.2611866394150404 2313 2 -0.15899170787989902 2313 3 -0.3042196867234317 2313 4 -0.2203021638083058 2313 5 -0.23120581255962946 2313 6 -0.30382555165199676 2313 7 -0.30901187651027584 2313 8 -0.30175471199250126 2313 9 -0.31162055467390104 2313 10-11 -0.392164632177078 2313 12-13 -0.3881426058779738 2313 14-15 -0.37722483462416534 2313 16-17 -0.3664639213090979 2313 18-19 -0.44962218166526924 2313 20-21 -0.35006719901250705 2313 22-23 -0.3938660895074477 2313 24-25 -0.25121153326885093 2313 26-27 -0.499595082630357 2313 28-29 -0.5544033200264167 2313 30-31 -0.6246446399416143 2313 32-33 -0.4085887414349969 2313 34-35 -0.47989176042189285 2313 36-37 -0.6057048055343586 2313 38-39 -0.7931367684831443 2313 40-41 -0.9483269749233116 2313 42-43 -0.6689719445633244 2313 44-45 -0.7216004993102771 2313 46-47 -0.5867250758553979 2313 48-49 -0.6377599918776724 2313 50-51 -0.5509830324938179 2313 52-53 -0.5637145007265971 2313 54-55 -0.6333457007855685 2313 56-57 0.46883875721864854 2313 58-59 4.040254493377702 2313 60-61 3.783779923900976 2313 62-63 1.0193806759590593 2313 64-65 0.9971632628938671 2313 66-67 1.6318742771307662 2313 68-69 0.20441881155408126 2313 70-71 2.6762189699346877 2313 72-73 8.33717444833719 2313 74-75 9.936239243856832 2313 76-77 8.665491680509202 2313 78-79 6.405610298193988 2313 80-81 3.4219038474135743 2313 82-83 1.824038698895098 2313 84-85 1.3666010583477721 2313 86-87 1.2106137586131709 2313 88-89 1.1990553350737336 2313 90-91 2.8127119026983536 2313 92-93 4.8648136573312115 2313 94-95 8.598786103638481 2313 96-97 9.435234892162445 2313 98-99 9.901864348381338 2313 100-101 9.026061711837663 2314 1 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3.9679768037164536 2314 60-61 3.4330340177055483 2314 62-63 1.1458871699310968 2314 64-65 1.0195928908778704 2314 66-67 1.8461821030884202 2314 68-69 -0.5197835924635612 2314 70-71 2.2616647213262873 2314 72-73 8.219140565416328 2314 74-75 9.862406822323077 2314 76-77 8.785414586165604 2314 78-79 6.237249238394039 2314 80-81 3.2379611712987675 2314 82-83 1.7335083851363216 2314 84-85 1.320609152136413 2314 86-87 1.0476825051446923 2314 88-89 1.2463917621234266 2314 90-91 2.5127252676946306 2314 92-93 4.63725202566874 2314 94-95 8.623651137090803 2314 96-97 9.226126912728606 2314 98-99 9.66977941781294 2314 100-101 8.826581508406491 2315 1 -0.7869911551185318 2315 2 -0.6704298428161124 2315 3 -0.5968021580129985 2315 4 -0.5198016528633005 2315 5 -0.7291332289870951 2315 6 -0.7348276169479249 2315 7 -0.7471038965834609 2315 8 -0.8529712515726331 2315 9 -1.1567048519238199 2315 10-11 -0.9393505247738219 2315 12-13 -0.8020915780074631 2315 14-15 -0.882565407174063 2315 16-17 -1.1080536300415176 2315 18-19 -0.9650167568336485 2315 20-21 -1.0460956976439348 2315 22-23 -1.0930311102841799 2315 24-25 -1.0869271558779516 2315 26-27 -1.04837216925133 2315 28-29 -1.0170665083648842 2315 30-31 -1.0717256251296376 2315 32-33 -0.980771898272323 2315 34-35 -1.0869951221554572 2315 36-37 -1.1098573760030064 2315 38-39 -1.1044027414429678 2315 40-41 -0.9034041279659704 2315 42-43 -1.156035619855409 2315 44-45 -1.055014498023489 2315 46-47 -1.0674000877643408 2315 48-49 -1.0062630259678969 2315 50-51 -0.9887130994499316 2315 52-53 -1.0844187627043098 2315 54-55 -1.092494681799046 2315 56-57 0.36566100781513455 2315 58-59 3.8457640352207108 2315 60-61 3.3538024091828227 2315 62-63 0.6566865324738593 2315 64-65 0.710764070596472 2315 66-67 1.6668469993536696 2315 68-69 5.942497956784848 2315 70-71 4.70504612384493 2315 72-73 8.572362537870209 2315 74-75 9.6556171888469 2315 76-77 8.535294229242158 2315 78-79 5.753849452382774 2315 80-81 2.8262107424758582 2315 82-83 1.2203446255101156 2315 84-85 0.3748781476293104 2315 86-87 0.6405351121813325 2315 88-89 0.8061104016886098 2315 90-91 2.262641663665857 2315 92-93 4.128399392329708 2315 94-95 8.116145914187427 2315 96-97 8.881363624854572 2315 98-99 9.1872357511469 2315 100-101 8.302507540446559 2316 1 -0.4656604999230822 2316 2 -0.4288779377143115 2316 3 -0.549652373001976 2316 4 -0.3307133704312122 2316 5 -0.6521657528004141 2316 6 -0.4380387566268027 2316 7 -0.45736478135663816 2316 8 -0.5623069694700931 2316 9 -0.7172964769292705 2316 10-11 -0.6395844121633942 2316 12-13 -0.5777448137548262 2316 14-15 -0.677183660508156 2316 16-17 -0.9481456842058762 2316 18-19 -1.0350169350978078 2316 20-21 -0.7293990071179763 2316 22-23 -0.800333773671106 2316 24-25 -1.9866108092717312 2316 26-27 -1.2361470182507546 2316 28-29 -1.0291425799432403 2316 30-31 -0.8074296830240613 2316 32-33 -0.7432914621611104 2316 34-35 -0.7174564794250031 2316 36-37 -0.7556231325669245 2316 38-39 -1.1167283226142288 2316 40-41 -0.7055873459375022 2316 42-43 -0.785903767013906 2316 44-45 -0.8493225958172772 2316 46-47 -0.7238739794129785 2316 48-49 -0.7902520983751842 2316 50-51 -1.0247713609938032 2316 52-53 -0.8510951335255541 2316 54-55 -0.7271616277462272 2316 56-57 0.6073766629973392 2316 58-59 3.8648891894252273 2316 60-61 3.7795394893075063 2316 62-63 1.2983955750194873 2316 64-65 0.9198102296904871 2316 66-67 6.1112436390261635 2316 68-69 8.956434419585577 2316 70-71 5.767689232020196 2316 72-73 9.458555810421892 2316 74-75 10.188270002782726 2316 76-77 8.801139624112924 2316 78-79 6.243055557785549 2316 80-81 3.1259740245405823 2316 82-83 1.7293166122617158 2316 84-85 1.4415502063851768 2316 86-87 1.3007369529755408 2316 88-89 1.2007185855068947 2316 90-91 2.5403639078766673 2316 92-93 4.420577233468883 2316 94-95 8.65270819354037 2316 96-97 9.394862979128806 2316 98-99 9.685715446980069 2316 100-101 8.627013766803973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 17.0 4 34.0 5 105.0 6 246.0 7 651.0 8 1490.0 9 2671.0 10 4404.0 11 6577.0 12 9502.0 13 12828.0 14 17077.0 15 23861.0 16 31965.0 17 41242.0 18 44911.0 19 46107.0 20 48270.0 21 48516.0 22 46192.0 23 44542.0 24 45662.0 25 48817.0 26 64940.0 27 93810.0 28 132426.0 29 174430.0 30 205077.0 31 218286.0 32 222715.0 33 230049.0 34 234179.0 35 227345.0 36 190809.0 37 118821.0 38 38245.0 39 1895.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66445171063534 19.486983026013785 14.477418612874452 23.37114665047643 2 15.893211477040877 20.742972449800547 39.57731098554637 23.786505087612213 3 19.655112295141024 24.55100796644231 29.852860307077062 25.9410194313396 4 13.113748603345698 16.821896104972407 36.758783942945946 33.305571348735945 5 13.337385232508533 37.948638255556546 34.40940269726486 14.304573814670066 6 32.363306084020664 36.44361004986706 17.06881117189466 14.124272694217616 7 28.76855874982221 32.70032026454396 20.42364962707977 18.107471358554058 8 26.297449972300164 35.712781795457225 19.295811874047118 18.693956358195493 9 26.27729705535063 16.585365489451505 19.929533587459396 37.20780386773847 10-11 25.16493866667065 26.71939203866917 27.258921508494904 20.856747786165272 12-13 25.929239417438833 23.900044909458725 27.77678020459749 22.393935468504957 14-15 22.568552835992286 25.476342512287125 26.115640743885553 25.839463907835036 16-17 22.121305562389242 28.647736253431205 26.750432948834202 22.48052523534535 18-19 21.919999283231327 27.28757001793975 29.04593049919765 21.746500199631274 20-21 22.776057845473385 26.3595190693476 29.057913042893947 21.806510042285073 22-23 22.35583895759285 26.421768880171637 28.992826059282727 22.229566102952788 24-25 22.216537506864277 26.667762960186625 29.14610805223235 21.969591480716744 26-27 22.104539520569347 26.715008181149276 29.12621667296943 22.05423562531195 28-29 21.8665898126347 27.111466843072552 29.085636123286513 21.93630722100624 30-31 22.18454371443217 26.647885073374777 28.956221539670018 22.211349672523035 32-33 21.928783377250543 26.72205268609253 28.997360487651818 22.35180344900511 34-35 22.38834581764628 26.799542314447894 28.881208946893917 21.93090292101191 36-37 22.393208639089647 26.522162793333287 28.927455119799717 22.157173447777353 38-39 21.99830844860839 26.810268053016877 28.978105051689408 22.213318446685324 40-41 22.262007740563234 26.679924218732058 28.96066256144345 22.097405479261255 42-43 21.954005583737295 26.777909491027764 28.697022784732606 22.571062140502335 44-45 22.081971513578054 26.788868693035788 28.774983032682737 22.35417676070341 46-47 22.363873591852112 27.04200348258353 28.24630671007884 22.347816215485512 48-49 22.277541346351512 27.0459855337949 28.23662154231876 22.43985157753483 50-51 22.023270081638337 27.27143561818366 28.31526222063897 22.390032079539036 52-53 22.280009334889147 27.294730455075843 27.953773628938155 22.47148658109685 54-55 22.056578334737846 27.129425759697774 28.099231257203815 22.714764648360564 56-57 22.091787510956884 27.08528488457628 28.140024529959284 22.682903074507553 58-59 20.765365713949368 26.81036627528576 29.733979205542582 22.690288805222288 60-61 22.07310152881942 27.00426917661226 28.396736306872977 22.525892987695343 62-63 21.8088897101782 27.056069794659006 28.360781195461254 22.774259299701534 64-65 21.059550485350513 27.082842674358705 28.806504287518308 23.051102552772477 66-67 21.31599886137722 26.85410411433469 28.92944096952518 22.90045605476291 68-69 22.000511517189125 26.23249184793445 29.167848959020343 22.59914767585608 70-71 21.18667812331023 26.58992877108425 29.34305177137702 22.8803413342285 72-73 21.068799244552544 26.60272177013139 29.35079874730754 22.977680238008528 74-75 21.455951550410738 26.915400283572776 28.609009396262152 23.019638769754337 76-77 20.030450758409955 27.168734302601962 29.239087707657625 23.561727231330465 78-79 20.99150920534236 27.312177973263452 28.209762342839333 23.486550478554854 80-81 21.632512268928643 27.312748113089917 27.529362926593993 23.525376691387446 82-83 21.86109818515899 27.276782806113143 27.343307128648597 23.51881188007927 84-85 21.804124914021518 27.223480595694404 27.231861456347197 23.740533033936888 86-87 21.73578026655636 27.337641356424356 27.223087190895544 23.70349118612373 88-89 21.698966895407587 27.3427029755234 27.011369600876833 23.946960528192175 90-91 21.461037564139257 27.31794552348671 27.252192847081535 23.9688240652925 92-93 21.243889510571883 27.262285718025158 27.38643534450119 24.107389426901765 94-95 19.94971590678181 27.285122074361556 28.45230581544744 24.312856203409194 96-97 19.708273765710025 27.18020335985018 28.660245804439295 24.4512770700005 98-99 19.9655414907254 27.302120380110416 27.963885099851833 24.76845302931235 100-101 19.85433338230488 27.387587811481527 27.97968755153256 24.778391254681033 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 308.0 1 244.5 2 193.5 3 223.5 4 252.5 5 317.5 6 438.5 7 644.5 8 938.5 9 1217.5 10 1431.5 11 1637.0 12 1859.5 13 2165.5 14 2476.5 15 2725.0 16 3056.0 17 3356.5 18 3714.0 19 4205.5 20 4819.5 21 5625.5 22 6531.0 23 7791.5 24 9514.5 25 11590.5 26 14512.5 27 18171.5 28 22158.0 29 26427.5 30 31145.0 31 36508.0 32 42429.0 33 47974.0 34 53623.0 35 59361.0 36 64783.5 37 69836.0 38 74175.0 39 78779.5 40 83104.0 41 87159.0 42 91433.5 43 95635.5 44 99719.0 45 103391.0 46 106486.5 47 108826.5 48 109717.0 49 108462.5 50 105680.5 51 101981.0 52 98305.5 53 94037.5 54 88595.5 55 81778.5 56 73182.0 57 64633.5 58 56272.5 59 47295.0 60 38630.5 61 30744.5 62 23772.0 63 17866.5 64 13001.0 65 9350.5 66 6691.0 67 4923.0 68 3489.0 69 2381.0 70 1658.5 71 1158.5 72 820.5 73 587.5 74 394.0 75 226.5 76 125.5 77 75.0 78 49.5 79 32.0 80 18.5 81 7.5 82 5.5 83 4.5 84 2.5 85 1.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021129471020333197 2 0.005898332899668984 3 0.004181096739005862 4 0.0 5 0.06678555416144186 6 3.733122088398091E-5 7 0.0 8 2.613185461878664E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.679904939779141E-4 16-17 0.0 18-19 0.0012879271204973414 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.4932488353592365E-4 28-29 0.0019412234859670075 30-31 0.007988881269171915 32-33 3.173153775138377E-4 34-35 0.0010452741847514655 36-37 0.027345119297516016 38-39 0.026430504385858485 40-41 0.028091743715195634 42-43 0.02865171202845535 44-45 0.0291370178999471 46-47 0.030835588450168237 48-49 0.030854254060610223 50-51 0.025833204851714794 52-53 0.022324070088620585 54-55 0.016948374281327335 56-57 0.150612810656421 58-59 0.7424993177719383 60-61 0.301169624481516 62-63 2.986497670718473E-4 64-65 0.13995474709404443 66-67 1.3788286433498351 68-69 2.6428637824814287 70-71 2.1370444051139295 72-73 1.8004474520135154 74-75 1.5156289022791831 76-77 1.0900529842018007 78-79 0.40883286551091697 80-81 1.8665610441990455E-4 82-83 1.306592730939332E-4 84-85 1.8665610441990456E-5 86-87 0.0028931696185085206 88-89 3.733122088398091E-5 90-91 0.21756635531184074 92-93 0.1691290962148755 94-95 1.2525557887097696 96-97 1.2289624571110935 98-99 0.9693984783047744 100-101 0.3922391378279874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2678723.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.149636131140724 #Duplication Level Percentage of deduplicated Percentage of total 1 76.40536232839847 43.6653865553594 2 13.576285652246941 15.517595700766783 3 4.417692002256786 7.5740847140527725 4 1.9463339375010047 4.449291052714912 5 1.014439618508697 2.898742753739262 6 0.6223253540712168 2.1339400524212007 7 0.38799523584807083 1.5521650584533362 8 0.27695371671015584 1.2662243308121945 9 0.19368960931597284 0.9962361625311587 >10 1.04133025962637 10.885628702129457 >50 0.07781048614899928 3.0554028105829834 >100 0.03690132999571098 3.9109898584961527 >500 0.002160352028620446 0.8184496540882186 >1k 5.891869168802404E-4 0.673062226948544 >5k 1.3093042597338674E-4 0.6028003669035543 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8657 0.32317637919262276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7455 0.27830425169007766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4937 0.18430423750421376 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3388 0.12647817635492734 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.1199366265194274E-4 0.0 0.0 0.0 0.0 3 1.1199366265194274E-4 0.0 0.0 0.0 0.0 4 1.1199366265194274E-4 0.0 0.0 0.0 0.0 5 1.4932488353592365E-4 0.0 0.0 0.0 0.0 6 1.4932488353592365E-4 0.0 0.0 0.0 0.0 7 1.8665610441990455E-4 0.0 0.0 0.0 0.0 8 1.8665610441990455E-4 0.0 0.0 0.0 0.0 9 1.8665610441990455E-4 0.0 0.0 0.0 0.0 10-11 1.8665610441990455E-4 0.0 0.0 3.733122088398091E-5 0.0 12-13 1.8665610441990455E-4 0.0 0.0 9.332805220995229E-5 0.0 14-15 2.2398732530388548E-4 0.0 0.0 1.1199366265194274E-4 0.0 16-17 2.2398732530388548E-4 0.0 0.0 1.1199366265194274E-4 0.0 18-19 2.799841566298568E-4 0.0 0.0 1.1199366265194274E-4 0.0 20-21 3.359809879558282E-4 0.0 0.0 1.4932488353592365E-4 0.0 22-23 3.733122088398091E-4 0.0 0.0 4.1064342972379006E-4 0.0 24-25 4.1064342972379E-4 0.0 0.0 9.332805220995228E-4 0.0 26-27 4.1064342972379E-4 0.0 0.0 0.001959889096408998 0.0 28-29 4.666402610497614E-4 0.0 0.0 0.006607626096464621 0.0 30-31 5.226370923757328E-4 0.0 0.0 0.02299603206453224 0.0 32-33 5.413027028177233E-4 0.0 0.0 0.0631644257356957 0.0 34-35 5.972995341436947E-4 0.0 0.0 0.12591820804166762 0.0 36-37 7.092931967956373E-4 0.0 0.0 0.22930702427985278 0.0 38-39 7.092931967956373E-4 0.0 0.0 0.3885060157395893 0.0 40-41 7.092931967956373E-4 0.0 0.0 0.5642987348822555 0.0 42-43 7.466244176796183E-4 0.0 0.0 0.7380942337076286 0.0 44-45 7.839556385635992E-4 0.0 0.0 0.9459918028105183 0.0 46-47 8.399524698895705E-4 0.0 0.0 1.180991838275178 0.0 48-49 8.58618080331561E-4 0.0 0.0 1.4143493000209428 0.0 50-51 8.58618080331561E-4 0.0 0.0 1.6495173259795806 0.0 52-53 8.58618080331561E-4 0.0 0.0 1.8693982169862282 0.0 54-55 8.58618080331561E-4 0.0 0.0 2.105984829338457 0.0 56-57 8.58618080331561E-4 0.0 0.0 2.3234018597667623 0.0 58-59 8.58618080331561E-4 0.0 0.0 2.4745933043468846 0.0 60-61 8.58618080331561E-4 0.0 0.0 2.586064329906452 0.0 62-63 8.58618080331561E-4 1.8665610441990456E-5 0.0 2.6823975453975644 1.8665610441990456E-5 64-65 8.58618080331561E-4 3.733122088398091E-5 0.0 2.7784134455111635 3.733122088398091E-5 66-67 8.58618080331561E-4 3.733122088398091E-5 0.0 2.8601688192470816 3.733122088398091E-5 68-69 8.58618080331561E-4 3.733122088398091E-5 3.733122088398091E-5 2.9580512804048795 3.733122088398091E-5 70-71 8.58618080331561E-4 3.733122088398091E-5 3.733122088398091E-5 3.1277030137121304 3.733122088398091E-5 72-73 8.772836907735514E-4 3.733122088398091E-5 3.733122088398091E-5 3.3415362469355734 3.733122088398091E-5 74-75 8.959493012155419E-4 3.733122088398091E-5 3.733122088398091E-5 3.590796808777914 7.466244176796182E-5 76-77 8.959493012155419E-4 3.733122088398091E-5 3.733122088398091E-5 3.9460780379307607 7.466244176796182E-5 78-79 8.959493012155419E-4 3.733122088398091E-5 3.733122088398091E-5 4.4535399890171545 7.466244176796182E-5 80-81 9.332805220995228E-4 3.733122088398091E-5 3.733122088398091E-5 5.031408622690737 7.466244176796182E-5 82-83 9.332805220995228E-4 3.733122088398091E-5 3.733122088398091E-5 5.571460729608847 7.466244176796182E-5 84-85 9.332805220995228E-4 3.733122088398091E-5 3.733122088398091E-5 5.997820603324794 7.466244176796182E-5 86-87 9.706117429835037E-4 7.466244176796182E-5 3.733122088398091E-5 6.374847268642558 9.332805220995229E-5 88-89 0.0010079429638674847 7.466244176796182E-5 3.733122088398091E-5 6.768206343097065 1.1199366265194274E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8425 0.0 30.283733 1 GTATCAA 12135 0.0 29.031057 1 TACATGG 11050 0.0 24.494398 2 GTACATG 11300 0.0 24.289948 1 TATCAAC 14875 0.0 23.55315 2 TCAACGC 14905 0.0 23.53606 4 ATCAACG 15010 0.0 23.371855 3 CAACGCA 14970 0.0 23.339375 5 AACGCAG 15335 0.0 22.830427 6 ACATGGG 11195 0.0 22.702078 3 CATGGGA 5800 0.0 20.892864 4 ACGCAGA 17235 0.0 20.176884 7 GAGTACT 8645 0.0 20.086573 12-13 CGCAGAG 17195 0.0 20.059774 8 AGAGTAC 14165 0.0 19.394075 10-11 GTACTTT 8940 0.0 19.266169 14-15 AGTACTT 9010 0.0 18.279139 12-13 GCAGAGT 18360 0.0 18.273613 9 GAGTACA 8860 0.0 17.618801 1 GTATAGC 1265 0.0 17.149416 1 >>END_MODULE