FastQCFastQC Report
Fri 27 May 2016
SRR939814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773252
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78970.2847559471696045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60870.21948961003183265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39320.14178300421310433No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28380.10233473193204223No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28030.10107267568904665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133500.030.3760591
GGTATCA90550.029.0941031
TACATGG110950.026.374022
GTACATG115150.026.024411
TATCAAC159950.025.0004582
TCAACGC161650.024.6811144
CAACGCA161700.024.5870845
ATCAACG162900.024.433083
ACATGGG117500.023.9837953
AACGCAG167850.023.7711686
ACGCAGA184450.021.4022457
CGCAGAG184100.021.3155278
GAGTACT87250.020.49153912-13
AGAGTAC141050.020.13150410-11
AGTACTT90500.019.96360812-13
TACCTGG19350.019.9363732
CATGGGG57800.019.6984544
CATGGGA62850.019.6877044
GTACTTT91000.019.54599414-15
GCAGAGT188150.019.4062379
Produced by FastQC (version 0.11.5)