FastQCFastQC Report
Fri 27 May 2016
SRR939814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773252
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90380.32589898069126066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75850.2735056172320438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50590.18242121523756225No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35700.12872973678554997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95900.029.3989471
GTATCAA135250.027.9695191
GTACATG122250.023.7610971
TACATGG120550.023.3409372
TATCAAC163950.022.8347552
ACATGGG119100.022.703053
TCAACGC165050.022.6768444
CAACGCA165850.022.6246645
ATCAACG166400.022.4928683
AACGCAG170750.022.003196
TATACCG1805.2324885E-621.0833025
CATGGGG57100.020.852534
GAGTACT91350.019.94085712-13
ACGCAGA191000.019.6455547
AGAGTAC151550.019.39128110-11
CGCAGAG193450.019.3231818
AGTACTT94900.019.06994812-13
GTACTTT94550.019.0151114-15
TACCTGG19750.018.2590122
GAGTACA96600.017.884971
Produced by FastQC (version 0.11.5)