##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939814_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2773252 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38761064627376 31.0 31.0 34.0 30.0 34.0 2 31.62322032040363 31.0 31.0 34.0 30.0 34.0 3 31.66622723070244 31.0 31.0 34.0 30.0 34.0 4 35.47572020140975 37.0 35.0 37.0 33.0 37.0 5 35.21259012884512 37.0 35.0 37.0 32.0 37.0 6 35.23557289420507 37.0 35.0 37.0 32.0 37.0 7 35.141661846813776 37.0 35.0 37.0 32.0 37.0 8 35.1020895324334 37.0 35.0 37.0 32.0 37.0 9 36.68109172913244 39.0 35.0 39.0 32.0 39.0 10-11 36.54706874817002 38.0 35.0 39.0 32.0 39.0 12-13 36.46734465529998 38.0 35.0 39.0 32.0 39.0 14-15 37.496162267258796 39.0 36.0 41.0 32.0 41.0 16-17 37.421397694836244 39.0 36.0 41.0 32.0 41.0 18-19 37.4064417874755 39.0 36.0 41.0 32.0 41.0 20-21 37.32652297735655 39.0 36.0 40.5 31.5 41.0 22-23 37.198465555961015 39.0 36.0 40.0 31.0 41.0 24-25 37.05589196365855 39.0 36.0 40.0 31.0 41.0 26-27 36.75663589172567 39.0 35.5 40.0 30.0 41.0 28-29 36.529301880968625 38.0 35.0 40.0 30.0 41.0 30-31 36.274691409219216 38.0 35.0 40.0 30.0 41.0 32-33 36.26252753085547 38.0 35.0 40.0 30.0 41.0 34-35 36.11794943265163 38.0 35.0 40.0 30.0 41.0 36-37 36.15417531475683 38.0 35.0 40.0 30.0 41.0 38-39 36.05875034075518 38.0 35.0 40.0 30.0 41.0 40-41 35.98076482050675 38.0 35.0 40.0 29.0 41.0 42-43 35.754714320948835 38.0 34.5 40.0 28.5 41.0 44-45 35.50349463373685 38.0 34.0 40.0 27.5 41.0 46-47 35.21106899048482 38.0 34.0 40.0 26.5 41.0 48-49 34.89560288787315 38.0 33.5 40.0 26.0 41.0 50-51 34.67004747494998 38.0 33.0 40.0 25.5 41.0 52-53 34.42723831083508 38.0 33.0 40.0 24.0 41.0 54-55 34.16180732944572 37.5 33.0 40.0 24.0 41.0 56-57 33.80021018645258 37.0 32.5 40.0 23.0 41.0 58-59 33.43989529260233 37.0 32.0 39.5 22.5 41.0 60-61 33.1287200009231 36.0 31.0 39.0 22.0 41.0 62-63 32.83251522039829 36.0 31.0 39.0 22.0 40.0 64-65 32.40108742371771 35.5 31.0 39.0 20.5 40.0 66-67 31.493496804473594 35.0 30.0 38.0 15.0 40.0 68-69 29.605927084880854 34.0 26.5 37.0 7.5 39.5 70-71 28.95222143534017 33.5 25.5 36.5 7.5 39.0 72-73 29.497322096946114 34.0 27.0 36.0 9.0 39.0 74-75 29.599621130715853 34.0 28.0 36.0 8.0 39.0 76-77 29.13485179132657 32.5 27.5 35.0 8.0 37.5 78-79 29.65813907282858 34.0 29.0 35.0 7.5 37.0 80-81 29.470197082702906 34.0 29.0 35.0 7.0 37.0 82-83 29.025105543960663 33.5 28.5 35.0 4.0 36.5 84-85 28.66534270956985 33.0 28.0 35.0 2.0 36.0 86-87 28.281186311233164 33.0 27.0 35.0 2.0 36.0 88-89 27.82290790739536 33.0 26.5 35.0 2.0 35.0 90-91 27.32875104750668 33.0 26.0 35.0 2.0 35.0 92-93 26.833658463060694 32.0 25.0 34.5 2.0 35.0 94-95 26.025588731207982 32.0 24.0 34.0 2.0 35.0 96-97 25.494639325961003 32.0 23.0 34.0 2.0 35.0 98-99 24.8199637104742 31.5 19.0 34.0 2.0 35.0 100-101 23.354236470396486 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2442240343069173 1101 2 0.302720754997992 1101 3 0.3096215274474936 1101 4 0.2736795821950935 1101 5 0.22801872798006428 1101 6 0.20302232542358922 1101 7 0.16096887939964688 1101 8 0.24278962363882783 1101 9 0.35167930725285146 1101 10-11 0.43793623086627065 1101 12-13 0.3238761826344998 1101 14-15 0.3557426791432121 1101 16-17 0.46791915644129034 1101 18-19 0.3917035725624416 1101 20-21 0.4176069174539805 1101 22-23 0.27236919330832876 1101 24-25 0.36522471281736557 1101 26-27 0.48618583323622744 1101 28-29 0.32915589724003524 1101 30-31 0.09762926659022497 1101 32-33 0.15938962453140704 1101 34-35 0.22132774815365508 1101 36-37 0.40728762559177056 1101 38-39 0.3480369763708495 1101 40-41 0.24127359663143721 1101 42-43 0.06310066403436565 1101 44-45 0.035027206030804336 1101 46-47 -0.021292411402072275 1101 48-49 0.2865426887401483 1101 50-51 0.24261598772540083 1101 52-53 0.043547828093558394 1101 54-55 -0.06771808795587475 1101 56-57 -0.0062399298442414874 1101 58-59 0.0072579079828827275 1101 60-61 -0.035493062502624184 1101 62-63 -0.11812876121054927 1101 64-65 -0.11651753685926991 1101 66-67 -0.21205759757401665 1101 68-69 -8.946759602708191 1101 70-71 -6.637875746124216 1101 72-73 -1.6248827251532205 1101 74-75 -0.5082142436834509 1101 76-77 -0.18565752927061752 1101 78-79 -0.33233486268425594 1101 80-81 -0.1832478403406803 1101 82-83 -0.3445301359699222 1101 84-85 -0.2579151623776035 1101 86-87 -0.3196575199750349 1101 88-89 -0.077388037235945 1101 90-91 -0.27272711993287757 1101 92-93 -0.5104005784224839 1101 94-95 -0.13997611526765397 1101 96-97 -0.6074463950642155 1101 98-99 -0.5649152961430275 1101 100-101 -0.6352994049080039 1102 1 0.2217851626049523 1102 2 0.13490795194785576 1102 3 0.2033444693081634 1102 4 0.1819321328862742 1102 5 0.313762839870833 1102 6 0.18506680329957703 1102 7 0.26401217365167184 1102 8 0.25123969080918584 1102 9 0.41473831760556834 1102 10-11 0.39844399693890864 1102 12-13 0.33891976039908656 1102 14-15 0.4738047642961263 1102 16-17 0.3005488296357086 1102 18-19 0.2525792084684113 1102 20-21 0.24890526976257377 1102 22-23 0.4320836568325106 1102 24-25 0.4013785044495677 1102 26-27 0.34959444079216695 1102 28-29 0.2899225938557919 1102 30-31 0.2119421849709724 1102 32-33 0.16526124332664693 1102 34-35 0.17145452988873444 1102 36-37 0.24360009412167472 1102 38-39 0.4436468745644362 1102 40-41 0.31975782050967183 1102 42-43 0.3722491881507466 1102 44-45 0.4198942726468289 1102 46-47 0.3907101254617231 1102 48-49 0.392575770394636 1102 50-51 0.47105069933127197 1102 52-53 0.323354646242791 1102 54-55 0.11603348785362044 1102 56-57 0.12466113615477781 1102 58-59 0.12249142277244829 1102 60-61 0.31342485774430884 1102 62-63 0.27722396815090633 1102 64-65 0.297533910836961 1102 66-67 0.5370157629388359 1102 68-69 -4.234975927705353 1102 70-71 0.5854094433355108 1102 72-73 0.9615883997893704 1102 74-75 0.5543015041690005 1102 76-77 0.333065975927866 1102 78-79 0.3497902032262665 1102 80-81 0.29742803392887396 1102 82-83 0.3078300036395234 1102 84-85 0.22119135517456456 1102 86-87 0.07296824467922747 1102 88-89 -0.021318717596429337 1102 90-91 -0.055306379514430404 1102 92-93 -0.11510459254239436 1102 94-95 0.20517563564771635 1102 96-97 -0.2737469945785591 1102 98-99 -0.08438343150306338 1102 100-101 -0.1347528648059324 1103 1 0.18673661110644346 1103 2 0.15649639224246314 1103 3 0.17020056691793783 1103 4 0.18243327152644184 1103 5 0.1731452442378867 1103 6 0.1349060750317932 1103 7 0.09722673497878276 1103 8 0.21435483949709777 1103 9 0.2973586764836753 1103 10-11 0.3117518463249951 1103 12-13 0.2726236301642686 1103 14-15 0.35005507633280786 1103 16-17 0.334253223098834 1103 18-19 0.3511528567133979 1103 20-21 0.2571493495252639 1103 22-23 0.17279711604403047 1103 24-25 0.06106978317355782 1103 26-27 0.10542920791645116 1103 28-29 0.13944940591443356 1103 30-31 0.14697994023511285 1103 32-33 0.26346904156489614 1103 34-35 0.014288750890067092 1103 36-37 0.17673360312209496 1103 38-39 0.35745445263471254 1103 40-41 0.3234388127319505 1103 42-43 0.010186524604250735 1103 44-45 0.12850511553060073 1103 46-47 0.10871147660240155 1103 48-49 0.02639999668800641 1103 50-51 -0.1138673909545247 1103 52-53 -0.08650874782204454 1103 54-55 -0.13319529979505518 1103 56-57 0.02013968931435528 1103 58-59 -0.12395894590418521 1103 60-61 -0.19718472531022257 1103 62-63 -0.22941256037096736 1103 64-65 -0.3684511428239503 1103 66-67 -0.08964776691148302 1103 68-69 -4.200293561917384 1103 70-71 0.4783762606257618 1103 72-73 0.700963810774752 1103 74-75 0.2651783477368035 1103 76-77 0.03795171510937578 1103 78-79 -0.14946030445859648 1103 80-81 0.04458915470154068 1103 82-83 -0.06811407938620206 1103 84-85 -0.21478556505455515 1103 86-87 -0.12360640826010894 1103 88-89 -0.18827549949141442 1103 90-91 -0.33052582573474965 1103 92-93 -0.1968069089389317 1103 94-95 -0.21256370538719693 1103 96-97 -0.6140780737279812 1103 98-99 -0.29002759272092504 1103 100-101 -0.030951972612641043 1104 1 0.1745329011817205 1104 2 0.17516224658269408 1104 3 0.23449187459985055 1104 4 0.17312102620797987 1104 5 0.2640144708925547 1104 6 0.23992271909016694 1104 7 0.2937657913992595 1104 8 0.1736520534261743 1104 9 0.27818911274704305 1104 10-11 0.1721943838297051 1104 12-13 0.1402279514213305 1104 14-15 0.288829386045407 1104 16-17 0.33822581378230865 1104 18-19 0.188105256368722 1104 20-21 0.2852104343083539 1104 22-23 0.28948914858296604 1104 24-25 0.27181785154660076 1104 26-27 0.24919623259176404 1104 28-29 0.2378840667906914 1104 30-31 0.16935222722396048 1104 32-33 0.22018463208588201 1104 34-35 0.2701655215883818 1104 36-37 0.3801567523895315 1104 38-39 0.43452095941326263 1104 40-41 0.2902552649709875 1104 42-43 0.2730429370155747 1104 44-45 0.369812069387649 1104 46-47 0.3272011937275039 1104 48-49 0.3518534801110178 1104 50-51 0.2786034743728578 1104 52-53 0.24738537939543193 1104 54-55 0.25823316155120324 1104 56-57 0.23932144310186487 1104 58-59 0.42006408272555973 1104 60-61 0.16920864453914675 1104 62-63 0.3029519602926527 1104 64-65 0.31965229529871664 1104 66-67 0.5721417196721816 1104 68-69 -3.7691331373685806 1104 70-71 0.8442679183914983 1104 72-73 0.9413608275883902 1104 74-75 0.5104195567579062 1104 76-77 0.46345317269669195 1104 78-79 0.12587841079508522 1104 80-81 0.20110174092907585 1104 82-83 0.12744023740589938 1104 84-85 0.10587907105373873 1104 86-87 0.07423689340357598 1104 88-89 0.0313033329620076 1104 90-91 -0.024992186928532334 1104 92-93 -0.0037989082193874424 1104 94-95 0.1373252891230301 1104 96-97 -0.25539768152791353 1104 98-99 -0.049429491611281406 1104 100-101 0.09570007887885268 1105 1 0.15164438159519733 1105 2 0.17874059260255848 1105 3 0.13178644302167442 1105 4 0.18576499400136015 1105 5 0.1772359280233573 1105 6 0.18829510432299656 1105 7 0.05431354429845214 1105 8 0.20380606611384167 1105 9 0.189512847660815 1105 10-11 0.17568414009643618 1105 12-13 0.18860160851841812 1105 14-15 0.2571718031340211 1105 16-17 0.23734185552918063 1105 18-19 0.25746555523920023 1105 20-21 0.25666344100176275 1105 22-23 0.21835289905376953 1105 24-25 0.2964646919902236 1105 26-27 0.292272512084061 1105 28-29 0.2717953050210724 1105 30-31 0.36810601432571843 1105 32-33 0.32023117476215646 1105 34-35 0.2874066490444136 1105 36-37 0.2416727717717322 1105 38-39 0.4348833777636756 1105 40-41 0.2391710888386811 1105 42-43 0.22699263591745478 1105 44-45 0.18495831048097955 1105 46-47 0.2339887806849248 1105 48-49 0.31852503275127475 1105 50-51 0.27195949474233316 1105 52-53 0.39279736834054546 1105 54-55 0.3920598522901031 1105 56-57 0.27330792280699967 1105 58-59 0.20387095882671247 1105 60-61 0.26122758297575643 1105 62-63 0.22427909649886857 1105 64-65 0.22275197961193527 1105 66-67 0.8303272050817014 1105 68-69 2.0650556191246956 1105 70-71 2.3469419815155064 1105 72-73 1.4150231002788551 1105 74-75 0.8389873028595467 1105 76-77 0.502778145222539 1105 78-79 0.43182252507952157 1105 80-81 0.4074384834882636 1105 82-83 0.4766452403910044 1105 84-85 0.23580157926072687 1105 86-87 0.2273594020135299 1105 88-89 0.3890236560558229 1105 90-91 0.21276917330636635 1105 92-93 0.32099240844849675 1105 94-95 0.5574862769157747 1105 96-97 0.03475554537430625 1105 98-99 0.20832148215192348 1105 100-101 0.15607774197057722 1106 1 0.3928595176755998 1106 2 0.3018084798410676 1106 3 0.30339772294758305 1106 4 0.21869675166388447 1106 5 0.3266444745002133 1106 6 0.35996735951484027 1106 7 0.33157961793585145 1106 8 0.317739202866413 1106 9 0.3084366460186274 1106 10-11 0.3647236590186296 1106 12-13 0.43035745752163024 1106 14-15 0.5593970200650062 1106 16-17 0.5338696222884209 1106 18-19 0.5563965998189317 1106 20-21 0.3860624622241744 1106 22-23 0.5260475317748288 1106 24-25 0.564629912144369 1106 26-27 0.6848081953997749 1106 28-29 0.48443290751033175 1106 30-31 0.5963007019245126 1106 32-33 0.6688111001572778 1106 34-35 0.7405794150516343 1106 36-37 0.8491641667192837 1106 38-39 0.8567703904647601 1106 40-41 0.6719824432986599 1106 42-43 0.5831342810668474 1106 44-45 0.7502515431574821 1106 46-47 0.9123679217414349 1106 48-49 0.8478945235868238 1106 50-51 0.8720661949012509 1106 52-53 0.7763016312984163 1106 54-55 0.9394910692397076 1106 56-57 0.8856749786969331 1106 58-59 0.941866338606232 1106 60-61 1.0248013656045671 1106 62-63 0.9936433001887721 1106 64-65 1.0251474683339339 1106 66-67 1.3652765883988423 1106 68-69 2.976871025882833 1106 70-71 3.2135958280778745 1106 72-73 1.985039949121699 1106 74-75 1.5088053854483796 1106 76-77 1.3037544838033703 1106 78-79 1.1450421463169036 1106 80-81 1.1422905555261451 1106 82-83 1.2645900500386809 1106 84-85 1.1183418429335141 1106 86-87 1.0726937385757545 1106 88-89 1.236465048662712 1106 90-91 1.2148507902078158 1106 92-93 1.2589252918586737 1106 94-95 1.4515724366712348 1106 96-97 1.3747209114232533 1106 98-99 1.7016583280102537 1106 100-101 1.9144881653054568 1107 1 0.1415432117596751 1107 2 0.08494361795637673 1107 3 0.08673974195279399 1107 4 0.11174655141211076 1107 5 0.12855523480061493 1107 6 0.05992377800757964 1107 7 -0.005256600922074028 1107 8 -0.002621893549793697 1107 9 0.052715719937850736 1107 10-11 0.10849747624671124 1107 12-13 0.09481785413959898 1107 14-15 0.06919642698895245 1107 16-17 0.06812882199017878 1107 18-19 0.08772934068880289 1107 20-21 0.2338070535884711 1107 22-23 0.12894176391986178 1107 24-25 0.22549437496947888 1107 26-27 0.11297760144422142 1107 28-29 0.23370613336886947 1107 30-31 0.24265212902196964 1107 32-33 0.1208644192434889 1107 34-35 0.3090264037926431 1107 36-37 0.17253324038909312 1107 38-39 0.19573993723020067 1107 40-41 0.23496710707127022 1107 42-43 0.10475064303489035 1107 44-45 0.18661217225391624 1107 46-47 0.1852195306472879 1107 48-49 0.1705620925132152 1107 50-51 0.18136277362165742 1107 52-53 0.14975445497314155 1107 54-55 0.17079217413593284 1107 56-57 0.22945850987543537 1107 58-59 0.17469161483029438 1107 60-61 0.30990403812235456 1107 62-63 0.4937708942401642 1107 64-65 0.24247978126491176 1107 66-67 0.8853120049369636 1107 68-69 2.202395283848592 1107 70-71 2.3829792285118465 1107 72-73 1.3257862955605475 1107 74-75 0.6567325071917445 1107 76-77 0.33140957555425743 1107 78-79 0.33693073518182715 1107 80-81 0.4014586213079703 1107 82-83 0.5122650685148749 1107 84-85 0.3937308932110817 1107 86-87 0.393223975871809 1107 88-89 0.5919429947135306 1107 90-91 0.39860906511524163 1107 92-93 0.3688805814328475 1107 94-95 0.6951497433078337 1107 96-97 0.25030030262109904 1107 98-99 0.5032287062373513 1107 100-101 0.41947056861555154 1108 1 -0.22486843622485964 1108 2 -0.19530468271488388 1108 3 -0.16344467118245376 1108 4 -0.2137636208936584 1108 5 -0.15412786732602513 1108 6 -0.0804214065114337 1108 7 -0.11401538405696243 1108 8 -0.13107283566985473 1108 9 -0.243855852413013 1108 10-11 -0.037247942696822633 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-0.4405069694237653 2316 60-61 -0.5003550395138419 2316 62-63 -0.4086896054320235 2316 64-65 -0.5410856389227732 2316 66-67 0.16060818179392555 2316 68-69 1.5729578323848266 2316 70-71 1.767784756985229 2316 72-73 0.841937822954943 2316 74-75 0.2804716538249963 2316 76-77 -0.06147601078761511 2316 78-79 -0.3456163011142017 2316 80-81 -0.4951628940627444 2316 82-83 -0.32517115396916196 2316 84-85 -0.47344349369012306 2316 86-87 -0.2639446290184644 2316 88-89 -0.2776125404127896 2316 90-91 -0.44648783800100134 2316 92-93 -0.3885004337334408 2316 94-95 -0.3612345402671444 2316 96-97 -0.3091509869608764 2316 98-99 -0.41290213655727115 2316 100-101 -0.635817310208985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 52.0 5 95.0 6 276.0 7 731.0 8 1592.0 9 2694.0 10 4423.0 11 6752.0 12 9371.0 13 12075.0 14 15127.0 15 18733.0 16 23199.0 17 27177.0 18 25136.0 19 21515.0 20 21286.0 21 22218.0 22 23742.0 23 27486.0 24 32961.0 25 40133.0 26 50012.0 27 61815.0 28 76171.0 29 92088.0 30 109105.0 31 130547.0 32 155466.0 33 188946.0 34 232342.0 35 293080.0 36 369222.0 37 412382.0 38 243766.0 39 21516.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.675076534218945 19.49400854644134 14.502080345781746 23.32883457355797 2 15.935259738667137 20.773250387007515 39.48490129685388 23.806588577471473 3 19.672067305820025 24.52809914136905 29.87297944795496 25.926854104855963 4 13.141250777066057 16.83673175030614 36.79380741454437 33.228210058083434 5 13.37487541701944 38.00119859284335 34.34866359061492 14.275262399522292 6 32.28179933291265 36.47197331650591 17.098674839989183 14.147552510592265 7 28.730926724293354 32.65988810248762 20.459590401449272 18.14959477176975 8 26.30940138148282 35.74943784409062 19.27628646801661 18.66487430640995 9 26.258378250516003 16.579849216731837 19.904393830780613 37.25737870197155 10-11 25.152672746652666 26.68109497441992 27.290596022287193 20.875636256640217 12-13 25.910267079948017 23.898747751736952 27.801061713829107 22.389923454485924 14-15 22.572849492220683 25.441773773173153 26.120255209407585 25.865121525198575 16-17 22.079807676358573 28.63516779278548 26.764685519691394 22.520339011164552 18-19 21.906700148417816 27.321516400240586 29.022443687050437 21.749339764291165 20-21 22.805320252180472 26.349985504382577 28.991865867220145 21.852828376216802 22-23 22.364772476500512 26.41624345713985 28.99395727470854 22.2250267916511 24-25 22.17514147212503 26.66298145911502 29.123578675485202 22.03829839327475 26-27 22.098772554845596 26.768599183626375 29.070112936493054 22.062515325034976 28-29 21.873030851724973 27.134586027901936 29.05951269783433 21.932870422538766 30-31 22.17791189269893 26.666446707618714 28.961142431236205 22.19449896844615 32-33 21.947190078144637 26.715667979594627 28.98568832799472 22.35145361426602 34-35 22.380462049156076 26.824331134327018 28.835746687021402 21.959460129495504 36-37 22.36981568753924 26.572057496781703 28.87598581548252 22.182141000196538 38-39 22.017572479983368 26.82782593254547 28.977195621605546 22.177405965865617 40-41 22.264850645309863 26.72396056672341 28.935621335435517 22.07556745253121 42-43 22.011602902440146 26.780993186069658 28.650054910298138 22.557349001192055 44-45 22.105951212856915 26.807681333898074 28.742885306053427 22.34348214719158 46-47 22.420303799807417 27.03188858556388 28.224823262253263 22.32298435237544 48-49 22.253589649567047 27.00222395140078 28.298482284902537 22.445704114129633 50-51 22.079061287737417 27.271039198606744 28.272654450948643 22.377245062707196 52-53 22.340533976159595 27.326021852171728 27.913633421961904 22.419810749706773 54-55 22.07182121601297 27.094875667960327 28.1555121508751 22.677790965151598 56-57 22.074412204185833 27.16567577243309 28.014781803376405 22.745130220004672 58-59 22.266741006223896 26.935509231977644 27.999644410720766 22.798105351077698 60-61 21.997703565171797 27.104097592710264 28.27867640535825 22.61952243675969 62-63 21.916399179933727 27.05746323593709 28.212811200673016 22.813326383456168 64-65 21.653701959770373 27.115412666413157 28.1064135497189 23.12447182409757 66-67 21.60252264082054 27.110407608803598 28.09493164146084 23.192138108915014 68-69 21.483633303739406 26.95544400302988 28.452821826703182 23.108100866527533 70-71 21.62346253213136 26.95200564313676 28.143443120725586 23.28108870400629 72-73 21.677167462068354 26.92748689312164 28.086379503237296 23.30896614157271 74-75 21.758366216284454 27.35675176558003 27.45527437726784 23.42960764086768 76-77 21.769310858576794 27.171122908817303 27.499612698022226 23.559953534583677 78-79 21.840769306219475 27.2196851461327 27.479394871291866 23.460150676355955 80-81 21.913882188849637 27.284318734920404 27.308208095987275 23.493590980242686 82-83 21.85246945866212 27.265217211794322 27.31326553921672 23.569047790326835 84-85 21.78829197863585 27.200293230599254 27.225858957956817 23.785555832808075 86-87 21.745987959822806 27.317085043893098 27.1848558442097 23.752071152074397 88-89 21.714631570331196 27.31716982608448 27.024227592028538 23.943971011555785 90-91 21.799361610368855 27.220228556757686 27.05406839971326 23.926341433160204 92-93 21.778810926357572 27.097787236620434 27.127175838314372 23.996225998707622 94-95 21.478404620255766 27.008460893897084 27.409312251948865 24.103822233898285 96-97 21.51168829482677 26.88392562826974 27.36119135566043 24.243194721243057 98-99 21.49720672405896 27.05146191659484 26.90793910694514 24.543392252401063 100-101 21.35907414993855 26.98088623918851 27.162676489086095 24.497363121786844 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 532.0 1 383.5 2 290.5 3 390.0 4 372.0 5 379.0 6 639.0 7 1262.5 8 2006.5 9 2451.0 10 2333.5 11 2117.0 12 2219.5 13 2444.5 14 2659.5 15 2880.0 16 3161.5 17 3413.0 18 3788.0 19 4220.5 20 4725.0 21 5477.0 22 6537.0 23 7776.5 24 9433.5 25 11693.0 26 14999.5 27 19308.5 28 22992.0 29 26996.5 30 31784.5 31 36885.0 32 43748.0 33 48770.5 34 53058.0 35 58529.5 36 64235.0 37 69950.0 38 73937.0 39 77336.0 40 80612.5 41 84840.0 42 88641.0 43 92919.5 44 97791.0 45 101182.0 46 103417.5 47 107180.0 48 112832.5 49 112506.5 50 107246.0 51 102844.0 52 99685.5 53 97945.5 54 95672.0 55 89151.5 56 80115.0 57 71631.5 58 64226.0 59 56070.0 60 46795.0 61 38538.5 62 30759.5 63 23388.5 64 17208.5 65 12415.0 66 8713.5 67 6614.0 68 4872.0 69 3301.0 70 2436.0 71 1701.5 72 1219.0 73 1000.0 74 758.0 75 473.5 76 288.5 77 163.0 78 97.0 79 60.5 80 33.0 81 23.5 82 13.0 83 7.5 84 4.0 85 2.0 86 1.5 87 2.5 88 2.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022933364872719825 2 0.02509688986071226 3 0.0 4 0.0 5 0.0 6 7.211749959974788E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.605874979987394E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.014687449968485E-5 26-27 4.327049975984872E-4 28-29 1.262056242995588E-4 30-31 3.064993732989285E-4 32-33 0.0021995837377923105 34-35 0.0038041981038867004 36-37 0.09975653132135126 38-39 0.0921661644884778 40-41 0.10096449943964703 42-43 0.10184793880974395 44-45 0.09611459759156397 46-47 0.08942569950368737 48-49 0.09043534449808384 50-51 0.08933555262918769 52-53 0.07026047398505438 54-55 0.05991161279249055 56-57 0.03333631418998346 58-59 0.014062912421950836 60-61 0.009303157448367476 62-63 0.0022176131126922473 64-65 0.005282606845681532 66-67 0.15321362789966436 68-69 0.4119351577137598 70-71 0.3951498096819186 72-73 0.18206062773956352 74-75 0.028558529841500157 76-77 0.03128096545139064 78-79 0.005625164968780335 80-81 0.0018389962397935707 82-83 9.014687449968485E-5 84-85 2.8846999839899154E-4 86-87 0.0013341737425953358 88-89 3.245287481988655E-4 90-91 8.654099951969745E-4 92-93 0.002451994986391428 94-95 0.07752631206972897 96-97 0.017236082404339746 98-99 0.018822667395534197 100-101 0.009952214944765208 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2773252.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.9192377369154 #Duplication Level Percentage of deduplicated Percentage of total 1 76.40309402427984 43.488058726038844 2 13.452465530660069 15.314081673746005 3 4.508177857640592 7.698061417180285 4 1.9096099765861838 4.347741769683778 5 1.0415712076738946 2.9642719594758242 6 0.6168160790226082 2.1065220625103898 7 0.4019726184226803 1.601598252221164 8 0.2889832401259531 1.3158964597370588 9 0.1982824463780123 1.0157477134002537 >10 1.0598542809725184 10.992120367964393 >50 0.07928993265849718 3.0999491570547586 >100 0.03709281409148449 3.96236985510204 >500 0.002092493615776374 0.8245185664719629 >1k 5.072711795641075E-4 0.48651828740380965 >5k 1.902266923365403E-4 0.7825437320095994 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9038 0.32589898069126066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7585 0.2735056172320438 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5059 0.18242121523756225 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3570 0.12872973678554997 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.6058749799873935E-5 0.0 0.0 0.0 0.0 6 3.6058749799873935E-5 0.0 0.0 0.0 0.0 7 3.6058749799873935E-5 0.0 0.0 0.0 0.0 8 3.6058749799873935E-5 0.0 0.0 0.0 0.0 9 3.6058749799873935E-5 0.0 0.0 0.0 0.0 10-11 3.6058749799873935E-5 0.0 0.0 0.0 0.0 12-13 3.6058749799873935E-5 0.0 0.0 0.0 0.0 14-15 3.6058749799873935E-5 0.0 0.0 3.6058749799873935E-5 0.0 16-17 3.6058749799873935E-5 0.0 0.0 3.6058749799873935E-5 0.0 18-19 7.211749959974787E-5 0.0 0.0 3.6058749799873935E-5 0.0 20-21 1.0817624939962182E-4 0.0 0.0 9.014687449968484E-5 0.0 22-23 1.0817624939962182E-4 0.0 0.0 2.8846999839899154E-4 0.0 24-25 1.4423499919949574E-4 0.0 0.0 6.310281214977939E-4 0.0 26-27 1.4423499919949574E-4 0.0 0.0 0.0014784087417948314 0.0 28-29 1.622643740994327E-4 0.0 0.0 0.005607135593880397 3.6058749799873935E-5 30-31 1.802937489993697E-4 0.0 0.0 0.02250065987512134 3.6058749799873935E-5 32-33 1.802937489993697E-4 0.0 0.0 0.06375186964617713 3.6058749799873935E-5 34-35 2.163524987992436E-4 0.0 0.0 0.12449283368406477 3.6058749799873935E-5 36-37 3.605874979987394E-4 0.0 0.0 0.22884685560489998 3.6058749799873935E-5 38-39 3.605874979987394E-4 0.0 0.0 0.3922651096979286 3.6058749799873935E-5 40-41 3.605874979987394E-4 0.0 0.0 0.5666812824799189 3.6058749799873935E-5 42-43 3.786168728986764E-4 0.0 0.0 0.7350576146704302 3.6058749799873935E-5 44-45 3.9664624779861334E-4 0.0 0.0 0.9461815947486922 3.6058749799873935E-5 46-47 4.5073437249842425E-4 0.0 0.0 1.196249024610818 3.6058749799873935E-5 48-49 4.687637473983612E-4 0.0 0.0 1.4612628062649913 7.211749959974787E-5 50-51 4.687637473983612E-4 0.0 0.0 1.7389872972236204 7.211749959974787E-5 52-53 4.687637473983612E-4 0.0 0.0 2.0024325232614997 1.0817624939962182E-4 54-55 4.687637473983612E-4 0.0 0.0 2.287170441056204 1.0817624939962182E-4 56-57 5.048224971982351E-4 0.0 0.0 2.6173063248489497 1.0817624939962182E-4 58-59 5.048224971982351E-4 0.0 0.0 2.9633621466783397 1.0817624939962182E-4 60-61 5.048224971982351E-4 0.0 0.0 3.3097424972559293 1.0817624939962182E-4 62-63 5.048224971982351E-4 0.0 0.0 3.6396620285498757 1.0817624939962182E-4 64-65 5.048224971982351E-4 0.0 0.0 3.985447409755767 1.0817624939962182E-4 66-67 5.048224971982351E-4 0.0 0.0 4.352435335843984 1.0817624939962182E-4 68-69 5.40881246998109E-4 0.0 0.0 4.765271962302741 1.0817624939962182E-4 70-71 5.58910621898046E-4 0.0 0.0 5.232196713461308 1.0817624939962182E-4 72-73 5.76939996797983E-4 0.0 0.0 5.701825870854867 1.4423499919949574E-4 74-75 5.76939996797983E-4 0.0 0.0 6.161124196430761 1.622643740994327E-4 76-77 5.76939996797983E-4 0.0 0.0 6.660808321782514 1.802937489993697E-4 78-79 5.76939996797983E-4 0.0 0.0 7.221612028045053 1.802937489993697E-4 80-81 6.49057496397731E-4 0.0 0.0 7.824334030949947 1.802937489993697E-4 82-83 6.49057496397731E-4 0.0 0.0 8.415931909541577 1.802937489993697E-4 84-85 6.670868712976679E-4 0.0 0.0 8.984037512638592 1.802937489993697E-4 86-87 6.851162461976049E-4 0.0 0.0 9.561987154430971 1.802937489993697E-4 88-89 6.851162461976049E-4 0.0 0.0 10.180899535996009 1.802937489993697E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9590 0.0 29.398947 1 GTATCAA 13525 0.0 27.969519 1 GTACATG 12225 0.0 23.761097 1 TACATGG 12055 0.0 23.340937 2 TATCAAC 16395 0.0 22.834755 2 ACATGGG 11910 0.0 22.70305 3 TCAACGC 16505 0.0 22.676844 4 CAACGCA 16585 0.0 22.624664 5 ATCAACG 16640 0.0 22.492868 3 AACGCAG 17075 0.0 22.00319 6 TATACCG 180 5.2324885E-6 21.083302 5 CATGGGG 5710 0.0 20.85253 4 GAGTACT 9135 0.0 19.940857 12-13 ACGCAGA 19100 0.0 19.645554 7 AGAGTAC 15155 0.0 19.391281 10-11 CGCAGAG 19345 0.0 19.323181 8 AGTACTT 9490 0.0 19.069948 12-13 GTACTTT 9455 0.0 19.01511 14-15 TACCTGG 1975 0.0 18.259012 2 GAGTACA 9660 0.0 17.88497 1 >>END_MODULE