FastQCFastQC Report
Fri 27 May 2016
SRR939813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2043525
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT83010.4062098579660146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56040.2742320255440966No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45160.2209906901065561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42930.2100781737438984No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32600.1595282661088071No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30180.1476859837780306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.13613731175297586No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24360.11920578412302273No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21620.10579758016173034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG89850.024.7122292
GTACATG89950.024.0512221
GAGTACT54400.022.32673312-13
ACATGGG93550.022.2144583
CATGGGA56900.020.0960254
GTACTTT59950.019.98475814-15
GTATTAG6400.019.2737791
AGTACTT58850.019.14702212-13
TAAGGTG8550.018.8676665
GTATAGG5650.018.4734361
CTAGTAC3609.822543E-1118.451473
GTAAGGT8100.017.5728264
ATGGGAT25000.017.4603615
ACTTTTT72900.017.25116-17
CATGGGG41050.016.9906334
AGAGTAC125400.016.4766710-11
TACTTTT68950.016.13746514-15
GTATTAC4751.8189894E-1115.9808481
TAGGGTG6350.015.6910125
TACCTGG14000.015.5888432
Produced by FastQC (version 0.11.5)