##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939813_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2043525 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.82086786312866 31.0 30.0 33.0 16.0 34.0 2 29.62551424621671 31.0 30.0 33.0 25.0 34.0 3 29.897250584162173 31.0 30.0 34.0 25.0 34.0 4 33.63656818487662 35.0 33.0 37.0 28.0 37.0 5 33.59991142755777 35.0 33.0 37.0 28.0 37.0 6 33.64102372126595 35.0 33.0 37.0 28.0 37.0 7 33.547310162586705 35.0 33.0 37.0 28.0 37.0 8 33.410735371478204 35.0 33.0 37.0 27.0 37.0 9 34.66226006532829 37.0 34.0 39.0 27.0 39.0 10-11 34.46952814377118 37.0 33.0 39.0 27.0 39.0 12-13 34.34373056360945 37.0 33.0 39.0 27.0 39.0 14-15 34.967579305366954 38.0 33.0 40.0 25.5 41.0 16-17 34.79729707246058 38.0 33.0 40.0 25.0 41.0 18-19 34.644457493791364 38.0 33.0 40.0 25.0 41.0 20-21 34.839413268739065 38.0 33.0 40.0 25.0 41.0 22-23 34.74411690583673 38.0 33.0 40.0 25.0 41.0 24-25 34.51913115817032 38.0 33.0 40.0 24.5 41.0 26-27 34.38955310064716 38.0 33.0 40.0 24.0 41.0 28-29 34.4511378622723 38.0 33.0 40.0 24.0 41.0 30-31 34.25469788722917 38.0 33.0 40.0 23.5 41.0 32-33 33.923291860877654 38.0 32.5 40.0 22.0 41.0 34-35 33.7210158916578 38.0 32.0 40.0 21.0 41.0 36-37 33.49987619432109 37.5 31.5 40.0 19.0 41.0 38-39 33.27779107179995 37.0 31.0 40.0 18.5 41.0 40-41 33.049623322445285 37.0 31.0 40.0 17.5 41.0 42-43 32.650782593802376 37.0 31.0 40.0 15.0 41.0 44-45 32.304308975911724 37.0 30.0 40.0 13.5 41.0 46-47 31.90528596420401 36.5 30.0 39.0 9.0 41.0 48-49 31.523760658665786 36.0 30.0 39.0 8.5 40.0 50-51 30.89082859275027 35.5 28.5 38.5 8.0 40.0 52-53 31.05179212390355 35.5 29.0 39.0 7.0 40.0 54-55 31.149811722391455 36.0 29.0 39.0 4.0 40.0 56-57 30.820766567573187 35.5 28.5 39.0 2.0 40.0 58-59 30.398494268482157 35.0 28.0 39.0 2.0 40.0 60-61 29.9418499896013 35.0 28.0 38.5 2.0 40.0 62-63 29.48886776525856 34.0 27.0 38.0 2.0 40.0 64-65 28.9215703257851 34.0 26.0 38.0 2.0 40.0 66-67 28.288727321662325 34.0 25.5 37.0 2.0 39.5 68-69 27.698952300559082 33.0 24.5 36.5 2.0 39.0 70-71 27.093508276140494 32.5 22.5 36.0 2.0 39.0 72-73 26.426123242925826 32.0 21.5 35.5 2.0 38.0 74-75 26.03743996280936 32.0 20.5 35.0 2.0 38.0 76-77 25.457350901016625 31.0 19.0 35.0 2.0 37.0 78-79 24.763731786985723 31.0 16.0 34.5 2.0 36.5 80-81 23.99350044653234 30.5 7.5 34.0 2.0 36.0 82-83 23.19907439351121 30.0 4.5 34.0 2.0 35.5 84-85 22.35941180068754 29.0 2.0 34.0 2.0 35.0 86-87 21.381913849842796 29.0 2.0 34.0 2.0 35.0 88-89 20.309981527018266 27.0 2.0 33.5 2.0 35.0 90-91 18.81684564661553 25.0 2.0 32.5 2.0 34.5 92-93 17.25028149888061 19.0 2.0 31.5 2.0 34.0 94-95 16.723985759900174 18.5 2.0 31.0 2.0 34.0 96-97 16.384535055847127 17.0 2.0 31.0 2.0 34.0 98-99 15.503136981441383 2.0 2.0 31.0 2.0 34.0 100-101 13.939417917568907 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.7288567805587292 1101 2 1.1242297478048506 1101 3 0.9825941809838596 1101 4 1.035681361864853 1101 5 0.9986863854887673 1101 6 1.2713412040322112 1101 7 1.082107884796379 1101 8 1.2883341680518043 1101 9 1.2053109115286702 1101 10-11 1.262194788748225 1101 12-13 1.2523007037068936 1101 14-15 1.2748477989609057 1101 16-17 1.2177851388875993 1101 18-19 1.3570702916784327 1101 20-21 1.3825470746149193 1101 22-23 1.4595379489692633 1101 24-25 1.4394225986114861 1101 26-27 1.456874078754261 1101 28-29 1.3554939292485528 1101 30-31 1.415172215369772 1101 32-33 1.4831358962674486 1101 34-35 1.1695188677648147 1101 36-37 1.242394913847022 1101 38-39 1.3801627497547742 1101 40-41 1.3823100542806799 1101 42-43 1.4507579510864588 1101 44-45 1.2313009188145472 1101 46-47 0.9241282898445711 1101 48-49 0.8596089627624544 1101 50-51 0.8404270182055278 1101 52-53 0.6046865880279491 1101 54-55 0.8902209482528143 1101 56-57 0.8348646900996464 1101 58-59 0.6481689489702447 1101 60-61 0.7238803285399165 1101 62-63 0.0870242383140507 1101 64-65 0.3933701609214957 1101 66-67 0.520805564663867 1101 68-69 0.691481411340451 1101 70-71 0.46346104888106865 1101 72-73 0.35225846056519927 1101 74-75 0.13560695281880442 1101 76-77 -0.11349633961575023 1101 78-79 -0.1284226834476776 1101 80-81 -0.3217895875772321 1101 82-83 -0.48525919412520224 1101 84-85 -0.5857937100772652 1101 86-87 -0.7582544750876288 1101 88-89 -4.6975331506890186 1101 90-91 -7.968491466177618 1101 92-93 -10.242814352741654 1101 94-95 -8.298203395396872 1101 96-97 -5.330996118095083 1101 98-99 -3.870985420039764 1101 100-101 -2.9665998662215447 1102 1 1.921435405983324 1102 2 1.0170099812855256 1102 3 0.9948108735813754 1102 4 0.9819444002734201 1102 5 1.0342339728894672 1102 6 0.9104146999571228 1102 7 0.8477548337143261 1102 8 1.0883025701963334 1102 9 1.1986650582320237 1102 10-11 1.1967219714902981 1102 12-13 1.1587933760955167 1102 14-15 1.4072874625124427 1102 16-17 1.4146638202953525 1102 18-19 1.3640939454208905 1102 20-21 1.3589585885986821 1102 22-23 1.3381867285021016 1102 24-25 1.5745480798777365 1102 26-27 1.5146397669177745 1102 28-29 1.268701145923977 1102 30-31 1.559950498026332 1102 32-33 1.7571494077852137 1102 34-35 1.747234136741561 1102 36-37 1.6661668048069416 1102 38-39 1.4722945402884733 1102 40-41 1.425379305102389 1102 42-43 1.4847359493623173 1102 44-45 1.5210910334941588 1102 46-47 1.6366667997309214 1102 48-49 1.2302758492402894 1102 50-51 1.3722670094130827 1102 52-53 1.4383324653664467 1102 54-55 1.5797084791208285 1102 56-57 1.6506131299555342 1102 58-59 1.481936752816253 1102 60-61 1.2781049961613853 1102 62-63 1.2297933095088247 1102 64-65 1.2147976283877249 1102 66-67 1.2727143155525553 1102 68-69 1.252820748266327 1102 70-71 1.1095530467241694 1102 72-73 1.2565032203012905 1102 74-75 0.9785621765968777 1102 76-77 0.8250698733469264 1102 78-79 0.8596268015416264 1102 80-81 0.8651838530514304 1102 82-83 0.6753901418046517 1102 84-85 0.4938105611285053 1102 86-87 0.38246073550177684 1102 88-89 -0.2825519891930668 1102 90-91 -5.915235341517208 1102 92-93 -10.008835667555894 1102 94-95 -8.19372268210963 1102 96-97 -4.930510472794749 1102 98-99 -3.3647208017384234 1102 100-101 -2.661665105489435 1103 1 2.088943676311313 1103 2 1.360051171165523 1103 3 0.9847988210626895 1103 4 0.874997698773825 1103 5 0.8495651226193388 1103 6 0.8223130324271111 1103 7 0.8420455490673078 1103 8 0.930262399497515 1103 9 0.97255737659804 1103 10-11 0.9637253497486427 1103 12-13 0.812533817765626 1103 14-15 0.9668032018489043 1103 16-17 0.9141373991921355 1103 18-19 0.8488342786276846 1103 20-21 0.7954146291639645 1103 22-23 0.8472083519735634 1103 24-25 0.7497615702834111 1103 26-27 0.8839291966064238 1103 28-29 0.7249199510322271 1103 30-31 0.6170837844835617 1103 32-33 0.8289189307149201 1103 34-35 0.7494718939445519 1103 36-37 0.9872639252928579 1103 38-39 0.9095113435004833 1103 40-41 0.6565899053346556 1103 42-43 0.4835506837300443 1103 44-45 0.5694315447303993 1103 46-47 0.6489702567186661 1103 48-49 0.7216159649594296 1103 50-51 0.5184270573239438 1103 52-53 0.5734353325381534 1103 54-55 0.4806266538327968 1103 56-57 0.4733436285314845 1103 58-59 0.6283290384562719 1103 60-61 0.41023201792698316 1103 62-63 0.4505839509945524 1103 64-65 0.1573516917415283 1103 66-67 0.22188031679053566 1103 68-69 0.28452219176348237 1103 70-71 0.21565974190297865 1103 72-73 0.457937133414692 1103 74-75 0.32996404160492787 1103 76-77 0.23449318510603234 1103 78-79 -0.12614112809934497 1103 80-81 -0.05495056198532566 1103 82-83 -0.3584255182413898 1103 84-85 -0.5263214228338846 1103 86-87 -0.5268492040392765 1103 88-89 -1.2612904906788316 1103 90-91 -6.9594516753448605 1103 92-93 -10.248541236399246 1103 94-95 -8.949459783857572 1103 96-97 -6.043396709734738 1103 98-99 -4.5478382057998115 1103 100-101 -3.7645782104763175 1104 1 1.9723260540858938 1104 2 1.4005972687707384 1104 3 0.9831719111439341 1104 4 0.8304668054665925 1104 5 0.6688108180705541 1104 6 0.8533611345479599 1104 7 0.9661378881275269 1104 8 0.8923634508518887 1104 9 1.023922698790308 1104 10-11 1.0196606392307217 1104 12-13 0.7343154913016434 1104 14-15 1.0030080064320828 1104 16-17 0.7706698998644796 1104 18-19 0.8478656378831673 1104 20-21 0.7222556720124373 1104 22-23 0.764838708033075 1104 24-25 0.914804584499521 1104 26-27 0.9680746819118511 1104 28-29 0.8310025301965211 1104 30-31 0.9946293004352569 1104 32-33 0.9009282621886356 1104 34-35 1.0519332890374002 1104 36-37 0.9412339451191087 1104 38-39 0.9229886621402841 1104 40-41 1.0082541849286173 1104 42-43 1.0032233159936723 1104 44-45 0.7943242828174419 1104 46-47 0.9103136730887762 1104 48-49 0.71600934098905 1104 50-51 0.7595040701265781 1104 52-53 0.6228371831970456 1104 54-55 0.6552427156006466 1104 56-57 0.5792577314403751 1104 58-59 0.42029799817207447 1104 60-61 0.6234916855714125 1104 62-63 0.7561474982054257 1104 64-65 0.7815265098966186 1104 66-67 0.4916440278650924 1104 68-69 0.3151246722343082 1104 70-71 0.28604802690069775 1104 72-73 0.13309849535954044 1104 74-75 0.14116548448092203 1104 76-77 0.3091355932767037 1104 78-79 0.08300005734806248 1104 80-81 -0.09456359039878848 1104 82-83 -0.1974837867901975 1104 84-85 -0.23786622891810438 1104 86-87 0.10506965842155935 1104 88-89 -0.5287920855352581 1104 90-91 -3.4068753567738526 1104 92-93 -7.945432517225319 1104 94-95 -6.0255883793206735 1104 96-97 -3.6714965164756634 1104 98-99 -2.6752562895954473 1104 100-101 -2.090285810749007 1105 1 2.0305467306754927 1105 2 1.2198231648756952 1105 3 0.9513100676204047 1105 4 0.8582296905691322 1105 5 0.7895498767626563 1105 6 0.8415853693516198 1105 7 0.8205179775156353 1105 8 0.8254840526709089 1105 9 0.9860595746302749 1105 10-11 0.6723358712559317 1105 12-13 0.7589195598469516 1105 14-15 0.9892835380204517 1105 16-17 0.8937928217352251 1105 18-19 0.7696986270153872 1105 20-21 0.6294000088823495 1105 22-23 0.5311708304516713 1105 24-25 0.6984768898135201 1105 26-27 0.759786928178734 1105 28-29 0.5919902190964095 1105 30-31 0.9846632958047721 1105 32-33 1.0165756287495924 1105 34-35 1.0457780395096279 1105 36-37 0.9560775680221525 1105 38-39 0.8732744412248792 1105 40-41 0.9669388317959076 1105 42-43 1.0074396794508118 1105 44-45 1.0281545439855435 1105 46-47 1.0967021819807066 1105 48-49 0.9431461126470673 1105 50-51 0.7878147118312491 1105 52-53 0.689508818541011 1105 54-55 0.7631499648206805 1105 56-57 0.8801603277345471 1105 58-59 0.6978656423057892 1105 60-61 0.6352443103553362 1105 62-63 0.3929908814899967 1105 64-65 0.5217953153384443 1105 66-67 0.7157647322632528 1105 68-69 0.8031314272312358 1105 70-71 0.44998152035366346 1105 72-73 0.39152941912480443 1105 74-75 0.1676994233517668 1105 76-77 0.05739174782500456 1105 78-79 -0.22489354804439543 1105 80-81 -0.2179813271342219 1105 82-83 -0.32870735563081865 1105 84-85 -0.3253299756053174 1105 86-87 0.12472718833179286 1105 88-89 -0.04563109317820846 1105 90-91 -2.901010915112936 1105 92-93 -7.566088130187797 1105 94-95 -5.354795306297948 1105 96-97 -2.7905298963703125 1105 98-99 -1.8073946560386016 1105 100-101 -1.3142072671805192 1106 1 1.5331185501009372 1106 2 0.9630789788291843 1106 3 0.688501130091904 1106 4 0.6302223946275021 1106 5 0.7183506063568217 1106 6 0.6598343433598259 1106 7 0.6883155151353364 1106 8 -0.4172245656601419 1106 9 -0.44264083576644 1106 10-11 -0.7157307042114667 1106 12-13 -0.454983768926418 1106 14-15 0.46021833054211214 1106 16-17 0.6827941897242837 1106 18-19 0.864399949404806 1106 20-21 0.5513607931960749 1106 22-23 0.49774629784793234 1106 24-25 0.6050021976110571 1106 26-27 0.6020441101212768 1106 28-29 0.4300111949286034 1106 30-31 0.6626523518923264 1106 32-33 0.6995578448918636 1106 34-35 0.808468417986596 1106 36-37 0.8152522146249765 1106 38-39 0.8260879387168956 1106 40-41 0.6145630908018376 1106 42-43 0.7154287355383673 1106 44-45 0.6362940788692626 1106 46-47 0.550081114949247 1106 48-49 0.3947147400980491 1106 50-51 0.4511389252375402 1106 52-53 0.48493061516663616 1106 54-55 0.5109794221850237 1106 56-57 0.5052218872420688 1106 58-59 0.4080617207371624 1106 60-61 0.22880108190116033 1106 62-63 0.6075052453750835 1106 64-65 0.5745951329399013 1106 66-67 0.5873743993859186 1106 68-69 0.257996908307387 1106 70-71 0.14870017060446017 1106 72-73 0.05964159422740423 1106 74-75 0.15704278860484422 1106 76-77 0.2042134532513593 1106 78-79 0.2944315773546009 1106 80-81 0.23798310515031673 1106 82-83 -0.3469426497484669 1106 84-85 -0.3707791320120677 1106 86-87 0.06693421067561545 1106 88-89 -0.0800496978293701 1106 90-91 -3.4956483980130724 1106 92-93 -8.545431495440875 1106 94-95 -6.2029758808533515 1106 96-97 -3.5677255188053323 1106 98-99 -2.5224835753272483 1106 100-101 -2.013258794768298 1107 1 1.84769219351179 1107 2 1.1343680493534407 1107 3 0.9960091577264301 1107 4 0.8077063123293797 1107 5 0.7028806789028508 1107 6 0.744333494646952 1107 7 0.5998971483400126 1107 8 0.6794990782912862 1107 9 0.7107020118004002 1107 10-11 0.7595272071980474 1107 12-13 0.8642359558095052 1107 14-15 0.8972756213641233 1107 16-17 1.0066210739209325 1107 18-19 1.0303835373520016 1107 20-21 0.8415302114223238 1107 22-23 0.9418804485404095 1107 24-25 0.7860590343446461 1107 26-27 0.826622510648896 1107 28-29 0.8761608494970616 1107 30-31 0.9404968374209588 1107 32-33 1.0003235244568032 1107 34-35 0.9684147923068238 1107 36-37 0.8748765105176375 1107 38-39 0.7640073488344186 1107 40-41 0.8307011769272776 1107 42-43 0.9151993127712359 1107 44-45 0.8327721907263808 1107 46-47 0.8286074899181592 1107 48-49 0.809926810200654 1107 50-51 0.8459399372060155 1107 52-53 0.7666669627821356 1107 54-55 0.7045617473339547 1107 56-57 0.8028686280765136 1107 58-59 0.8830764786107288 1107 60-61 0.7201499407337231 1107 62-63 0.5324084272374954 1107 64-65 0.5643912256171859 1107 66-67 0.6795117503205823 1107 68-69 0.9328639793289781 1107 70-71 0.5879573035766832 1107 72-73 0.6280287154149526 1107 74-75 0.5372917804204427 1107 76-77 0.5263515610000553 1107 78-79 0.23912058084675536 1107 80-81 0.18654753705327565 1107 82-83 0.010224811572932424 1107 84-85 0.33408685784662495 1107 86-87 0.5280257527382552 1107 88-89 0.3098120214358744 1107 90-91 -3.0653721608924247 1107 92-93 -4.247585171935313 1107 94-95 -2.5522016601015096 1107 96-97 -1.1253959371072568 1107 98-99 -0.7922370701863191 1107 100-101 -0.4594274778037697 1108 1 1.6237736184481761 1108 2 1.017454768555691 1108 3 -0.32558414470681285 1108 4 -0.4761409474428575 1108 5 0.35632201434128064 1108 6 0.6671719860327272 1108 7 0.5816837965860273 1108 8 0.5266672065644542 1108 9 0.5684574192643126 1108 10-11 0.5525863559696091 1108 12-13 0.5826926337844966 1108 14-15 0.49703373556086206 1108 16-17 0.7642828959825749 1108 18-19 0.7260361060865463 1108 20-21 0.4814866273039087 1108 22-23 0.5590875536827653 1108 24-25 0.4213024659875373 1108 26-27 0.3753924460826923 1108 28-29 0.2546312385789449 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2.025579876247132 2311 88-89 2.392702347522686 2311 90-91 3.0352320159962503 2311 92-93 3.7886770316909093 2311 94-95 3.44293620658679 2311 96-97 2.707073596485955 2311 98-99 2.549152491584218 2311 100-101 2.4397230171666795 2312 1 1.3593813094740135 2312 2 0.7653680508649572 2312 3 0.08764153990406598 2312 4 -0.45974336434591834 2312 5 0.06910423298921131 2312 6 -0.1742237043615802 2312 7 0.2240499689531532 2312 8 0.3896299045760827 2312 9 0.385283639546536 2312 10-11 0.35897403444416653 2312 12-13 0.33225491371219107 2312 14-15 0.47244810187254416 2312 16-17 0.27274072187520915 2312 18-19 -0.24075931997594324 2312 20-21 0.5415898948281921 2312 22-23 0.5453532065421243 2312 24-25 0.7609983738497306 2312 26-27 0.5477962785396002 2312 28-29 0.6151034944781841 2312 30-31 0.6157087685742866 2312 32-33 0.6249368185251925 2312 34-35 0.7458274302896015 2312 36-37 0.6432854619056769 2312 38-39 0.7607809036441466 2312 40-41 0.658334140391915 2312 42-43 0.5893811437518934 2312 44-45 0.46856219582883796 2312 46-47 0.7367186893451638 2312 48-49 0.8122082321426838 2312 50-51 0.7466107925076564 2312 52-53 0.66075256182841 2312 54-55 0.6497099118209029 2312 56-57 0.74521929486151 2312 58-59 0.7576995435695544 2312 60-61 0.7925141269761795 2312 62-63 0.7918527755925204 2312 64-65 0.7611507233005668 2312 66-67 0.9568877879513593 2312 68-69 1.0484833037102312 2312 70-71 1.1401197149935776 2312 72-73 1.0964748933557154 2312 74-75 1.3759219080595777 2312 76-77 1.5482716089886246 2312 78-79 1.2545236068645345 2312 80-81 1.5009121711372053 2312 82-83 1.626258292149803 2312 84-85 1.9517941297427548 2312 86-87 2.3355936258777668 2312 88-89 2.918221400367848 2312 90-91 3.585202766937943 2312 92-93 4.4283271323256805 2312 94-95 4.188313504428407 2312 96-97 3.4017834411099805 2312 98-99 3.00319254992794 2312 100-101 2.79230250963119 2313 1 -7.357363986263493 2313 2 -2.8867333197510803 2313 3 -1.4900085816056823 2313 4 -0.793350029377379 2313 5 -0.7938865681053358 2313 6 -0.3905436724924485 2313 7 -0.2673817237811349 2313 8 -0.3501533175110296 2313 9 -0.31144538240872066 2313 10-11 -0.12042019118995739 2313 12-13 -0.18671728517064423 2313 14-15 -0.22987682667415754 2313 16-17 -0.2990516860705057 2313 18-19 -0.5971776570754699 2313 20-21 -0.5905689167595085 2313 22-23 -1.02386222928385 2313 24-25 -0.16575884236803518 2313 26-27 -0.48501298420848116 2313 28-29 -0.5326998058552945 2313 30-31 -0.028363021333007055 2313 32-33 -0.44943919369362817 2313 34-35 -0.13447405218150976 2313 36-37 0.09262766101466724 2313 38-39 -0.10556239661974587 2313 40-41 -0.11848106911455147 2313 42-43 -0.3053536440452973 2313 44-45 -0.22851952621739713 2313 46-47 -1.1245646040647834 2313 48-49 -0.6978396976686945 2313 50-51 -0.8435884504265978 2313 52-53 -1.2045395964873968 2313 54-55 -0.7998998566234405 2313 56-57 -0.6990543082112879 2313 58-59 -0.3309806100702595 2313 60-61 -0.049747389486345384 2313 62-63 -0.3964902689529346 2313 64-65 -0.4802829264625892 2313 66-67 -0.2834702570029002 2313 68-69 -0.41988088979382354 2313 70-71 -0.2196468810460921 2313 72-73 -0.1821232781704687 2313 74-75 -0.0254563403908854 2313 76-77 0.04837685365201949 2313 78-79 0.003530523439437161 2313 80-81 0.23467350668328635 2313 82-83 0.4542086001426604 2313 84-85 0.5902066270681523 2313 86-87 1.1255434346108117 2313 88-89 1.6639597525669387 2313 90-91 2.3223110567382577 2313 92-93 3.32636429760851 2313 94-95 3.1308654657192463 2313 96-97 2.3005736276104862 2313 98-99 1.7630298816886896 2313 100-101 1.3579601757046564 2314 1 0.37614747806463456 2314 2 -0.12497680843183545 2314 3 -1.306494699209754 2314 4 -0.32210018386503947 2314 5 -0.2480420176241367 2314 6 -3.6083162021675186 2314 7 -3.0041347423357756 2314 8 -2.3598840253600777 2314 9 -3.30662335219899 2314 10-11 -2.8061210815762543 2314 12-13 -1.4709359671624895 2314 14-15 -1.393202865502957 2314 16-17 -2.340422475274103 2314 18-19 -3.4310621083621484 2314 20-21 -3.3489790909141206 2314 22-23 -4.260322057117428 2314 24-25 -3.465893112299632 2314 26-27 -2.075153514248946 2314 28-29 -2.0685093749556103 2314 30-31 -2.231401122021353 2314 32-33 -2.4916767930359143 2314 34-35 -1.9651491901457092 2314 36-37 -2.2179184058637205 2314 38-39 -3.6561762499988575 2314 40-41 -2.9793481344937263 2314 42-43 -2.040517790547959 2314 44-45 -2.54655656564265 2314 46-47 -1.9126179385802793 2314 48-49 -2.5034911923032723 2314 50-51 -3.427054105803574 2314 52-53 -2.409080929326368 2314 54-55 -2.8454717027913397 2314 56-57 -1.8948499599880329 2314 58-59 -1.2615451214324942 2314 60-61 -2.1201192077121327 2314 62-63 -1.6368935623612835 2314 64-65 -2.125085558395053 2314 66-67 -1.4339355335492563 2314 68-69 -1.3427707226247385 2314 70-71 -1.2416314833276765 2314 72-73 -2.2256004731043504 2314 74-75 -2.4154198141333545 2314 76-77 -1.6423791418127571 2314 78-79 -2.3689049813187815 2314 80-81 -1.4532476401256709 2314 82-83 -1.0115841365190086 2314 84-85 -1.9641975475915707 2314 86-87 -0.5130115146366307 2314 88-89 0.2848661652827502 2314 90-91 1.330307136781677 2314 92-93 2.3680648843929646 2314 94-95 2.1719138458824965 2314 96-97 1.4219421599507989 2314 98-99 1.1542960894598213 2314 100-101 1.2329494287366458 2315 1 -3.041981536727029 2315 2 -2.2096701822249543 2315 3 -3.050429712608679 2315 4 -4.168470023179271 2315 5 -4.397523892035689 2315 6 -3.38622484045732 2315 7 -6.182739796623988 2315 8 -3.3248076975682466 2315 9 -2.871600542898065 2315 10-11 -4.005842172542728 2315 12-13 -3.1758684754713684 2315 14-15 -2.3180455795322743 2315 16-17 -2.707051162632972 2315 18-19 -2.3680204387396557 2315 20-21 -1.5457342247561883 2315 22-23 -1.0677783073195073 2315 24-25 -2.5582909288996305 2315 26-27 -4.070230078506601 2315 28-29 -3.5849778929084373 2315 30-31 -3.5553628078007335 2315 32-33 -4.322436147551755 2315 34-35 -4.483156907618266 2315 36-37 -3.4283464524484515 2315 38-39 -2.1383729065650563 2315 40-41 -1.8916871145345624 2315 42-43 -3.2990485366555653 2315 44-45 -6.953717185298942 2315 46-47 -3.621220131149368 2315 48-49 -2.5590467880741024 2315 50-51 -3.604336399193972 2315 52-53 -3.6076528687467224 2315 54-55 -2.3961637031523146 2315 56-57 -1.8426627958358672 2315 58-59 -3.1318641234887785 2315 60-61 -3.5548792503917994 2315 62-63 -5.279181446479516 2315 64-65 -4.646461586840449 2315 66-67 -3.9988971609360497 2315 68-69 -2.42987580659182 2315 70-71 -3.963293587168991 2315 72-73 -3.0495497323630723 2315 74-75 -2.0855493781048473 2315 76-77 -3.6037601698214097 2315 78-79 -2.1802787223192084 2315 80-81 -1.9120328524213122 2315 82-83 -1.4979977268376174 2315 84-85 -2.8601903445528443 2315 86-87 -2.31839796495154 2315 88-89 -1.6204380769625004 2315 90-91 -0.7840254191099199 2315 92-93 -0.10074442811400175 2315 94-95 -0.16916084788219266 2315 96-97 -0.6364723800917957 2315 98-99 -1.0659846196095106 2315 100-101 -1.2261323310131065 2316 1 -7.140659981890746 2316 2 -5.992897024421371 2316 3 -3.6818753129397166 2316 4 -4.519136815678671 2316 5 -2.3663019905025706 2316 6 -3.2191818855175356 2316 7 -3.710793928207 2316 8 -3.0383560458580448 2316 9 -7.441332965119436 2316 10-11 -7.2517392130190395 2316 12-13 -6.401742295659847 2316 14-15 -5.082683746467374 2316 16-17 -5.106276772774816 2316 18-19 -4.430441944521913 2316 20-21 -3.094323377075682 2316 22-23 -3.6396338976634333 2316 24-25 -3.4378879288259974 2316 26-27 -4.701604638653784 2316 28-29 -4.626388097597506 2316 30-31 -7.279933273959159 2316 32-33 -5.102437661692903 2316 34-35 -5.5222658251836485 2316 36-37 -4.464490609056277 2316 38-39 -6.812035821411207 2316 40-41 -4.113986109704868 2316 42-43 -4.884842781467299 2316 44-45 -3.2899039660170786 2316 46-47 -4.2772505612380485 2316 48-49 -5.382375295514656 2316 50-51 -5.022274875283504 2316 52-53 -6.572128109946295 2316 54-55 -5.472678648036538 2316 56-57 -4.638704353824878 2316 58-59 -3.322861102466071 2316 60-61 -3.4954253575452903 2316 62-63 -4.6533970228435635 2316 64-65 -3.4056128070204217 2316 66-67 -4.541730502738897 2316 68-69 -3.7262632317533217 2316 70-71 -3.0140886149783697 2316 72-73 -2.356104719021001 2316 74-75 -2.490582393641386 2316 76-77 -3.2019994103448077 2316 78-79 -5.00120741975336 2316 80-81 -5.997303111059438 2316 82-83 -3.6280375611947235 2316 84-85 -2.187847895605845 2316 86-87 -2.2916541877086694 2316 88-89 -1.2580465428782475 2316 90-91 -1.208615486136594 2316 92-93 -1.1436628107208762 2316 94-95 -1.5078737034590546 2316 96-97 -2.9030234465760714 2316 98-99 -4.452384624587507 2316 100-101 -3.932527658746883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17692.0 3 6063.0 4 6038.0 5 6462.0 6 7447.0 7 8464.0 8 9976.0 9 12178.0 10 14184.0 11 16946.0 12 19181.0 13 21398.0 14 23385.0 15 26115.0 16 28935.0 17 31118.0 18 29872.0 19 29737.0 20 32422.0 21 35706.0 22 39096.0 23 43666.0 24 48732.0 25 54650.0 26 61883.0 27 69870.0 28 78782.0 29 89298.0 30 100520.0 31 112786.0 32 124617.0 33 137505.0 34 154573.0 35 176707.0 36 185217.0 37 141804.0 38 39881.0 39 619.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.913318418476386 19.192000854517428 13.424596186617983 22.470084540388196 2 15.433194691436045 20.423323192388903 40.12580649902265 24.017675617152403 3 20.507349547641002 24.484120646050762 29.105159136495516 25.903370669812713 4 12.714940130601867 16.112872079844813 37.45775675830618 33.71443103124714 5 13.606134749306008 37.97502711781035 33.871394458070036 14.547443674813604 6 31.935810754525967 35.958639303246116 17.546200517645723 14.559349424582194 7 28.860422963094397 32.65690542687942 20.429835043805 18.052836566221185 8 26.285579019644455 34.384340894020504 19.529087841099617 19.800992245235424 9 26.658941994377066 17.159702698145686 20.174187438977047 36.007167868500204 10-11 25.359425389886 26.192325788839117 27.15277845849221 21.295470362782673 12-13 26.966539642961457 23.58877251583386 27.247760913159304 22.196926928045382 14-15 23.32264949411041 25.08732078227267 26.238700492289745 25.351329231327174 16-17 22.36767211268627 28.418529126309938 26.672330023936546 22.541468737067245 18-19 22.220032937301493 27.634101870368195 28.414402227188955 21.731462965141358 20-21 23.41729774771463 26.176408597407054 28.167855304757005 22.238438350121314 22-23 22.75202075053997 26.31817658062286 28.20099879134969 22.728803877487476 24-25 23.012148052241038 26.279076610708135 28.42994884772512 22.278826489325702 26-27 22.766369460742432 26.20980043810815 28.84512003829768 22.178710062851746 28-29 22.135332622477875 27.26197704793566 28.56662896582303 22.03606136376344 30-31 22.548031534679566 26.539743259646155 28.296857566729194 22.615367638945084 32-33 22.401217258886984 26.63837675928117 28.351661286189483 22.608744695642365 34-35 23.108875699601363 26.47051756156958 28.24462276082203 22.17598397800703 36-37 23.024383449719405 26.10393482837816 28.59828635632234 22.273395365580097 38-39 22.57593217346959 26.644126473530395 28.591883569032817 22.188057783967206 40-41 22.59874510534691 26.71167581642936 28.474437467358765 22.215141610864965 42-43 22.23716686235537 26.740597686991403 27.99661759697869 23.025617853674536 44-45 22.534903612853974 26.486368413581797 28.087684808528717 22.891043165035516 46-47 22.952064137120875 26.844019461615588 27.663608186275603 22.540308214987938 48-49 22.752229129383693 26.782297847791277 27.771449483532702 22.69402353929233 50-51 22.29192733519964 27.033545694636825 28.132357843309197 22.54216912685434 52-53 22.611594330875533 27.27984066012235 27.49400267855829 22.614562330443828 54-55 22.0378540678418 26.814315993138848 27.809074958785757 23.338754980233595 56-57 22.528619839901047 26.966788650792463 27.533778191612107 22.970813317694386 58-59 22.959021523117094 26.583089904282847 27.4257892888602 23.032099283739853 60-61 22.356160246099474 26.73252592975584 28.223086976443497 22.68822684770119 62-63 22.304565063592012 26.61301512078339 28.112909955320614 22.969509860303987 64-65 21.9996724878519 27.121315304475985 27.822034409367628 23.056977798304487 66-67 22.157406955674546 26.799589991818962 27.445124876714384 23.59787817579211 68-69 22.012680227185975 26.790209929077317 27.740920516535017 23.456189327201688 70-71 22.32983239324944 26.63932798426171 27.39319305055466 23.637646571934194 72-73 22.159193606494938 26.290780679301328 27.795423337706097 23.754602376497637 74-75 21.84982850612093 27.243602242112324 27.346241052826493 23.560328198940258 76-77 22.165590503204943 26.465992678621713 27.34920384181035 24.019212976362997 78-79 22.072796647294325 26.573984546275568 27.57125063711906 23.78196816931105 80-81 22.354860685048685 26.681019161218046 27.24781676823068 23.71630338550259 82-83 22.214726575148905 26.405957158697298 27.561573333595003 23.8177429325588 84-85 21.917159478880055 26.585862876519116 27.429355952704743 24.067621691896086 86-87 21.823280098084254 26.559784132238136 27.602824508603835 24.014111261073783 88-89 21.913828844782238 26.3935094481785 27.25429879613106 24.438362910908204 90-91 21.84452359518696 26.087501465337308 27.5897763323879 24.478198607087833 92-93 22.345142652393964 26.0257255173724 27.64892652024824 23.980205309985397 94-95 21.865249687781333 25.959137959775287 27.718045490266167 24.457566862177217 96-97 21.825569444659124 25.835032975887035 27.472136534721407 24.867261044732434 98-99 22.040708442840828 26.121713026510285 26.820859438636084 25.016719092012803 100-101 22.03420825448848 25.74853415462076 27.06826751276225 25.14899007812851 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2554.0 1 1521.0 2 490.5 3 479.5 4 418.0 5 402.5 6 559.0 7 943.0 8 1405.5 9 1651.0 10 1581.5 11 1423.0 12 1427.0 13 1513.5 14 1648.5 15 1791.5 16 1847.0 17 1971.5 18 2189.5 19 2495.5 20 2849.5 21 3273.5 22 3941.0 23 4793.5 24 5689.5 25 6989.0 26 8901.5 27 11462.0 28 13945.5 29 16727.0 30 20208.0 31 23978.0 32 28664.0 33 32612.5 34 35735.5 35 39246.5 36 43431.0 37 48128.0 38 52242.0 39 54939.0 40 57395.5 41 60577.5 42 63206.5 43 66013.0 44 69193.5 45 72901.0 46 77325.5 47 85451.5 48 98070.5 49 98384.0 50 87021.5 51 79307.0 52 74834.0 53 72426.5 54 69815.0 55 64903.0 56 59044.5 57 53266.5 58 47705.5 59 41607.0 60 34875.0 61 28605.5 62 23123.0 63 17999.5 64 13647.0 65 10467.5 66 7996.0 67 6168.5 68 4672.5 69 3389.0 70 2517.5 71 1865.5 72 1365.5 73 1031.5 74 776.5 75 571.5 76 390.0 77 253.0 78 177.5 79 115.0 80 77.0 81 63.5 82 43.0 83 23.0 84 12.5 85 13.5 86 11.0 87 5.0 88 5.0 89 6.0 90 4.5 91 2.0 92 1.5 93 2.5 94 2.5 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12757367783609203 2 0.11230594193856204 3 0.11974406968351256 4 0.13046084584235573 5 0.1189611088682546 6 0.1344245849695991 7 0.16378561554177218 8 0.15179652805813484 9 0.16192608360553457 10-11 0.15585813728728545 12-13 0.14489668587367419 14-15 0.14974125591808274 16-17 0.14296375136100611 18-19 0.16075164238264764 20-21 0.16515579696847357 22-23 0.19867630687170454 24-25 0.22094175505560243 26-27 0.2328819074882862 28-29 0.22837988280055296 30-31 0.22020772929129814 32-33 0.2210640926829865 34-35 0.21179090052727517 36-37 0.21913115817031845 38-39 0.20577188925997972 40-41 0.21091006961011 42-43 0.20290918877919284 44-45 0.19649869710426834 46-47 0.21196217320561284 48-49 0.24766029287627994 50-51 0.24939748718513355 52-53 0.2503027856277755 54-55 0.24915281193036545 56-57 0.2250278318102299 58-59 0.22515016943761396 60-61 0.19693911256285093 62-63 0.18996586780195984 64-65 0.19114030902484677 66-67 0.198358229040506 68-69 0.19821142388764512 70-71 0.20396129237469565 72-73 0.2333467904723456 74-75 0.23598928322384116 76-77 0.25010704542396106 78-79 0.24721987741769735 80-81 0.2603100035477912 82-83 0.2596004453089637 84-85 0.2349127121028615 86-87 0.2382892306186614 88-89 0.2412987362523091 90-91 0.4409292766176093 92-93 0.5758676796222214 94-95 0.33723590364688466 96-97 0.27386501266194446 98-99 0.30258988757172045 100-101 0.3210384017812358 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2043525.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.569504272743615 #Duplication Level Percentage of deduplicated Percentage of total 1 79.62020132683043 49.021763257889916 2 11.635499768474546 14.327839054212015 3 3.8838378370993993 7.173779109178044 4 1.7648487749041513 4.346434567488299 5 0.9200675357425303 2.83240510365562 6 0.5166453972024125 1.9085760594328358 7 0.34508189318201343 1.4872564767701502 8 0.2263281597778349 1.1147930080386885 9 0.17306573080082033 0.9590014126805944 >10 0.8466313193588296 9.220752707348158 >50 0.04387992624511958 1.8456500250832921 >100 0.020347366132544363 2.3435435508219284 >500 0.0021065697837577544 0.9174810810371695 >1k 0.0012963506361586036 1.8070765637544153 >5k 1.6204382951982545E-4 0.6936480226088376 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8301 0.4062098579660146 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5604 0.2742320255440966 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4516 0.2209906901065561 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4293 0.2100781737438984 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3260 0.1595282661088071 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3018 0.1476859837780306 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2782 0.13613731175297586 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2436 0.11920578412302273 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2162 0.10579758016173034 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.8935050953621806E-5 0.0 0.0 0.0 6 0.0 4.8935050953621806E-5 0.0 0.0 0.0 7 0.0 4.8935050953621806E-5 0.0 0.0 0.0 8 0.0 4.8935050953621806E-5 0.0 0.0 0.0 9 0.0 4.8935050953621806E-5 0.0 4.8935050953621806E-5 0.0 10-11 0.0 4.8935050953621806E-5 0.0 7.340257643043271E-5 0.0 12-13 0.0 4.8935050953621806E-5 0.0 1.7127267833767632E-4 0.0 14-15 0.0 4.8935050953621806E-5 0.0 1.9574020381448723E-4 4.8935050953621806E-5 16-17 0.0 4.8935050953621806E-5 0.0 1.9574020381448723E-4 4.8935050953621806E-5 18-19 0.0 4.8935050953621806E-5 0.0 2.2020772929129813E-4 4.8935050953621806E-5 20-21 0.0 4.8935050953621806E-5 0.0 2.9361030572173084E-4 4.8935050953621806E-5 22-23 0.0 4.8935050953621806E-5 0.0 5.13818035013029E-4 4.8935050953621806E-5 24-25 0.0 4.8935050953621806E-5 0.0 9.542334935956252E-4 4.8935050953621806E-5 26-27 0.0 4.8935050953621806E-5 0.0 0.001932934512668061 4.8935050953621806E-5 28-29 0.0 4.8935050953621806E-5 0.0 0.006532829302308512 4.8935050953621806E-5 30-31 0.0 4.8935050953621806E-5 0.0 0.026963213075445615 4.8935050953621806E-5 32-33 0.0 4.8935050953621806E-5 0.0 0.07034413574583134 4.8935050953621806E-5 34-35 0.0 4.8935050953621806E-5 0.0 0.13770323338349175 4.8935050953621806E-5 36-37 0.0 4.8935050953621806E-5 0.0 0.24394122900380472 4.8935050953621806E-5 38-39 0.0 4.8935050953621806E-5 0.0 0.4014142229725597 4.8935050953621806E-5 40-41 0.0 4.8935050953621806E-5 0.0 0.5762346925043735 4.8935050953621806E-5 42-43 0.0 4.8935050953621806E-5 0.0 0.7511530321380947 4.8935050953621806E-5 44-45 0.0 4.8935050953621806E-5 0.0 0.9596652842514772 4.8935050953621806E-5 46-47 0.0 4.8935050953621806E-5 0.0 1.2137605363281585 4.8935050953621806E-5 48-49 0.0 4.8935050953621806E-5 0.0 1.468981294576773 4.8935050953621806E-5 50-51 0.0 4.8935050953621806E-5 0.0 1.7435313979520681 4.8935050953621806E-5 52-53 0.0 4.8935050953621806E-5 0.0 2.0064104916749246 7.340257643043271E-5 54-55 0.0 4.8935050953621806E-5 0.0 2.293072020161241 1.2233762738405452E-4 56-57 0.0 4.8935050953621806E-5 0.0 2.6366694804320963 1.4680515286086542E-4 58-59 0.0 4.8935050953621806E-5 0.0 3.00206750590279 1.4680515286086542E-4 60-61 0.0 4.8935050953621806E-5 0.0 3.3982701459487896 1.4680515286086542E-4 62-63 0.0 4.8935050953621806E-5 0.0 3.7683170012600775 1.4680515286086542E-4 64-65 4.8935050953621806E-5 4.8935050953621806E-5 0.0 4.134302247342215 1.4680515286086542E-4 66-67 4.8935050953621806E-5 4.8935050953621806E-5 0.0 4.547118337186969 1.4680515286086542E-4 68-69 4.8935050953621806E-5 4.8935050953621806E-5 0.0 5.003315349702108 1.4680515286086542E-4 70-71 4.8935050953621806E-5 4.8935050953621806E-5 0.0 5.493130742222386 1.4680515286086542E-4 72-73 4.8935050953621806E-5 4.8935050953621806E-5 0.0 5.9715687353959455 2.9361030572173084E-4 74-75 4.8935050953621806E-5 4.8935050953621806E-5 0.0 6.442544133299078 2.9361030572173084E-4 76-77 4.8935050953621806E-5 4.8935050953621806E-5 0.0 6.913813141507934 2.9361030572173084E-4 78-79 4.8935050953621806E-5 4.8935050953621806E-5 0.0 7.460442128185366 2.9361030572173084E-4 80-81 4.8935050953621806E-5 4.8935050953621806E-5 0.0 8.016588982273278 3.1807783119854174E-4 82-83 4.8935050953621806E-5 4.8935050953621806E-5 0.0 8.525978395175004 3.9148040762897445E-4 84-85 4.8935050953621806E-5 4.8935050953621806E-5 0.0 9.014007658335474 3.9148040762897445E-4 86-87 4.8935050953621806E-5 4.8935050953621806E-5 0.0 9.500519934916383 3.9148040762897445E-4 88-89 4.8935050953621806E-5 4.8935050953621806E-5 0.0 10.021898435301747 3.9148040762897445E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 8985 0.0 24.712229 2 GTACATG 8995 0.0 24.051222 1 GAGTACT 5440 0.0 22.326733 12-13 ACATGGG 9355 0.0 22.214458 3 CATGGGA 5690 0.0 20.096025 4 GTACTTT 5995 0.0 19.984758 14-15 GTATTAG 640 0.0 19.273779 1 AGTACTT 5885 0.0 19.147022 12-13 TAAGGTG 855 0.0 18.867666 5 GTATAGG 565 0.0 18.473436 1 CTAGTAC 360 9.822543E-11 18.45147 3 GTAAGGT 810 0.0 17.572826 4 ATGGGAT 2500 0.0 17.460361 5 ACTTTTT 7290 0.0 17.251 16-17 CATGGGG 4105 0.0 16.990633 4 AGAGTAC 12540 0.0 16.47667 10-11 TACTTTT 6895 0.0 16.137465 14-15 GTATTAC 475 1.8189894E-11 15.980848 1 TAGGGTG 635 0.0 15.691012 5 TACCTGG 1400 0.0 15.588843 2 >>END_MODULE