FastQCFastQC Report
Fri 27 May 2016
SRR939813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2043525
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT92840.45431301305342486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60850.29776978505278867No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52720.25798558862749416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51960.25426652475501893No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38080.18634467403139185No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37820.18507236270659766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35690.1746491968534762No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26030.12737793763227756No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24970.12219082223119365No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23610.11553565530150109No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22030.10780391725082883No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21300.10423165853121444No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC21190.1036933729707246No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG90850.025.8558441
TACATGG91350.025.1383322
ACATGGG93800.023.5265943
ATGGGAG22850.020.8415475
TACCTGG15200.020.7848662
GTAAGGT7800.020.5531854
CATGGGA59200.020.150864
GAGTACT57650.019.53396612-13
CATGGGG37450.018.885754
GTATAGG5950.018.2332951
AGTACTT63750.018.18221312-13
GTACTTT63400.018.0224714-15
TAGCCCT12050.017.9997124
GGACCGC2102.0982323E-517.949266
GTGTAGC12650.017.5251481
CGCGGAA2300.017.41358682-83
TACGCGG1405.5298733E-717.3842264-65
CGCATAG4350.017.34513366-67
ACAACCG4355.456968E-1217.343055
GTACCTG19600.016.6053241
Produced by FastQC (version 0.11.5)