##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939813_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2043525 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40754431680552 31.0 31.0 34.0 30.0 34.0 2 31.611418015439007 31.0 31.0 34.0 30.0 34.0 3 31.67785028321161 31.0 31.0 34.0 30.0 34.0 4 35.472048543570544 37.0 35.0 37.0 33.0 37.0 5 35.2085181243195 37.0 35.0 37.0 32.0 37.0 6 35.240458521427435 37.0 35.0 37.0 32.0 37.0 7 35.160529477251316 37.0 35.0 37.0 32.0 37.0 8 35.103657650383525 37.0 35.0 37.0 32.0 37.0 9 36.70098971140553 39.0 35.0 39.0 32.0 39.0 10-11 36.55085868780661 38.0 35.0 39.0 32.0 39.0 12-13 36.495974113358045 38.0 35.0 39.0 32.0 39.0 14-15 37.51246228942636 39.0 36.0 41.0 32.0 41.0 16-17 37.44599943724691 39.0 36.0 41.0 32.0 41.0 18-19 37.43398661626357 39.0 36.0 41.0 32.0 41.0 20-21 37.347266855066614 39.0 36.0 40.5 31.5 41.0 22-23 37.2067968339022 39.0 36.0 40.0 31.0 41.0 24-25 37.03287163112759 39.0 36.0 40.0 31.0 41.0 26-27 36.72538872781101 39.0 35.5 40.0 30.0 41.0 28-29 36.51048360064105 38.0 35.0 40.0 30.0 41.0 30-31 36.26783401230716 38.0 35.0 40.0 30.0 41.0 32-33 36.24670605938269 38.0 35.0 40.0 30.0 41.0 34-35 36.345093649453766 38.0 35.0 40.0 30.0 41.0 36-37 36.31866211570693 38.0 35.0 40.0 30.0 41.0 38-39 36.1829556770776 38.0 35.0 40.0 30.0 41.0 40-41 36.03197538566937 38.0 35.0 40.0 29.5 41.0 42-43 35.83532156445358 38.0 35.0 40.0 29.0 41.0 44-45 35.59483319264506 38.0 34.0 40.0 28.0 41.0 46-47 35.30027305758432 38.0 34.0 40.0 27.0 41.0 48-49 34.98697471281242 38.0 34.0 40.0 26.0 41.0 50-51 34.711654371735115 38.0 33.0 40.0 26.0 41.0 52-53 34.428032933289295 38.0 33.0 40.0 24.5 41.0 54-55 34.119107914021114 37.5 33.0 40.0 24.0 41.0 56-57 32.70645159711773 36.5 31.5 39.5 16.0 41.0 58-59 29.0406388470902 34.0 23.0 38.5 7.0 40.0 60-61 29.049444709509302 34.0 24.0 38.0 8.5 40.0 62-63 31.092248687928947 35.0 29.0 38.0 8.5 40.0 64-65 30.631652903683587 35.0 29.0 38.0 4.0 40.0 66-67 24.95967262450912 28.0 17.5 36.0 2.0 39.0 68-69 17.178122606770167 13.0 7.0 29.0 2.0 35.5 70-71 16.04609828604984 12.5 7.5 25.5 2.0 33.0 72-73 18.05920260334471 16.5 7.0 29.0 2.0 34.0 74-75 18.79161302161706 17.5 7.5 30.5 2.0 35.0 76-77 19.821650089918155 22.5 7.5 30.5 2.0 34.5 78-79 22.920130167235534 27.5 11.5 33.5 2.0 35.5 80-81 25.632838110617683 31.5 21.0 34.5 2.0 36.0 82-83 26.737137788869724 32.5 25.5 35.0 2.0 36.0 84-85 26.760254217589704 33.0 25.5 35.0 2.0 36.0 86-87 26.496623726159456 33.0 25.0 35.0 2.0 35.5 88-89 26.050539386599134 33.0 24.5 35.0 2.0 35.0 90-91 24.294795267980575 31.5 11.0 34.0 2.0 35.0 92-93 22.02181769246767 30.5 2.0 34.0 2.0 35.0 94-95 17.49874359256677 20.0 2.0 33.0 2.0 35.0 96-97 16.14611516864242 10.5 2.0 32.0 2.0 34.5 98-99 15.027304535055848 2.0 2.0 31.5 2.0 34.0 100-101 14.412883375539815 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.370391792662204 1101 2 0.4553231288845083 1101 3 0.40501945496008673 1101 4 0.22753659476283872 1101 5 0.5235736888530909 1101 6 0.5336998785966145 1101 7 0.3761539320461722 1101 8 0.5003250736942988 1101 9 0.49854006798285155 1101 10-11 0.46182631650174244 1101 12-13 0.5668417130508203 1101 14-15 0.5763190112109626 1101 16-17 0.5829681268339542 1101 18-19 0.5283520588679664 1101 20-21 0.5781323190393266 1101 22-23 0.6296586166841891 1101 24-25 0.5520001320186338 1101 26-27 0.593978555022268 1101 28-29 0.5462980188454267 1101 30-31 0.6621870949944082 1101 32-33 0.6560207183957161 1101 34-35 0.8765517927652198 1101 36-37 0.8757869340764017 1101 38-39 0.7465899932554763 1101 40-41 0.7767206270281903 1101 42-43 0.7275142819824794 1101 44-45 0.6218591300515541 1101 46-47 0.4052573839840292 1101 48-49 0.7327953589190273 1101 50-51 0.466739301911133 1101 52-53 0.5060043875637845 1101 54-55 0.439634499230138 1101 56-57 1.109948170221223 1101 58-59 -7.91701630980997 1101 60-61 -9.58231857255318 1101 62-63 -2.51985765539445 1101 64-65 -0.034474012165865986 1101 66-67 -1.1791939723401477 1101 68-69 -3.6109149778733975 1101 70-71 -4.044010485284643 1101 72-73 -6.73649475060159 1101 74-75 -7.70801000386059 1101 76-77 -8.604743941521697 1101 78-79 -11.546087758637274 1101 80-81 -7.479318372561536 1101 82-83 -3.3632284625183146 1101 84-85 -2.380380487800224 1101 86-87 -2.154103297300008 1101 88-89 -2.1722798863901787 1101 90-91 -8.20247192168563 1101 92-93 -14.18291870172369 1101 94-95 -12.26985277328457 1101 96-97 -12.040732993174522 1101 98-99 -11.615703369445171 1101 100-101 -11.473119040022105 1102 1 0.42114756528128794 1102 2 0.4391567043535787 1102 3 0.41580737496255793 1102 4 0.3549171668900968 1102 5 0.45194615887805867 1102 6 0.4359972487620567 1102 7 0.43850817835145506 1102 8 0.5906262493405947 1102 9 0.5358014955876982 1102 10-11 0.6590999711028402 1102 12-13 0.6169672458353119 1102 14-15 0.7502806610311623 1102 16-17 0.8098132526930257 1102 18-19 0.8526353542495286 1102 20-21 0.7988364703793138 1102 22-23 0.8390545137713517 1102 24-25 0.7969144228356839 1102 26-27 0.8667745187396818 1102 28-29 0.6948062222669265 1102 30-31 0.6887584872577435 1102 32-33 0.6971391450972817 1102 34-35 0.7679856828390328 1102 36-37 0.7626837842510525 1102 38-39 0.8119426012806414 1102 40-41 0.8244316715090392 1102 42-43 0.9068097871875409 1102 44-45 0.8307346928883561 1102 46-47 0.9393555914926353 1102 48-49 0.9929384422515 1102 50-51 0.828311501293598 1102 52-53 0.8739030271387307 1102 54-55 0.7306799417546728 1102 56-57 1.3318733039110597 1102 58-59 -8.764268155021693 1102 60-61 -10.018059868546093 1102 62-63 -2.8054078187072804 1102 64-65 0.016695623668852022 1102 66-67 -1.3527383243952116 1102 68-69 -3.8171682621613634 1102 70-71 -4.235510662163945 1102 72-73 -6.888138697723267 1102 74-75 -7.550316097781781 1102 76-77 -8.837560031699226 1102 78-79 -11.877782811855617 1102 80-81 -8.098847564515076 1102 82-83 -3.939770832838313 1102 84-85 -2.9861079425586254 1102 86-87 -2.7352960476529056 1102 88-89 -2.9986668433449672 1102 90-91 -8.703416422800142 1102 92-93 -14.565007182588097 1102 94-95 -12.612169170854283 1102 96-97 -12.348346702522337 1102 98-99 -11.74697872071372 1102 100-101 -11.405769241458433 1103 1 0.14393262277619812 1103 2 0.1398365168990452 1103 3 0.16912111632990445 1103 4 0.10500131946432134 1103 5 0.07550153968715989 1103 6 0.08098558895537877 1103 7 0.21833278568619363 1103 8 0.3057508512521636 1103 9 0.2694036652280687 1103 10-11 0.16333401437222506 1103 12-13 0.1860430682397194 1103 14-15 0.3364091844526982 1103 16-17 0.2401841271130749 1103 18-19 0.21431084602782136 1103 20-21 0.22575849179895613 1103 22-23 0.2772320588724497 1103 24-25 0.1463175473272571 1103 26-27 0.027725433478160255 1103 28-29 0.4144793876940511 1103 30-31 0.33301960895545335 1103 32-33 0.2738028414897542 1103 34-35 0.2138824734468514 1103 36-37 -0.10361483957385076 1103 38-39 0.04181564309698871 1103 40-41 0.09603714367042926 1103 42-43 0.08747087111009222 1103 44-45 -0.004390544842671318 1103 46-47 -0.02071272637221 1103 48-49 -0.04033723877657991 1103 50-51 0.06934691456689279 1103 52-53 0.03857430380808324 1103 54-55 0.11493801058399811 1103 56-57 0.5848658629534427 1103 58-59 -6.539945676747077 1103 60-61 -8.849964283377144 1103 62-63 -2.8291823234693787 1103 64-65 -0.4361344040266957 1103 66-67 -2.0239428796675547 1103 68-69 -4.337419345392 1103 70-71 -4.953071130463668 1103 72-73 -7.982641454187586 1103 74-75 -8.481037140413477 1103 76-77 -9.458553784773134 1103 78-79 -12.39327892400669 1103 80-81 -9.015567138216834 1103 82-83 -5.333844715058369 1103 84-85 -4.5123162098945215 1103 86-87 -4.154382412504805 1103 88-89 -4.13777876252858 1103 90-91 -9.493418465837639 1103 92-93 -14.822976782003142 1103 94-95 -12.539535681495844 1103 96-97 -12.50458413807549 1103 98-99 -12.077581809487206 1103 100-101 -11.717828141715191 1104 1 0.11088313219210377 1104 2 0.08638146370589439 1104 3 0.12628671246222112 1104 4 0.07155780552653113 1104 5 -0.08617859465604027 1104 6 0.055691478666368255 1104 7 0.047687838253914094 1104 8 0.12017445137475136 1104 9 0.15958763660821518 1104 10-11 0.11337044589659229 1104 12-13 0.05236478205367945 1104 14-15 0.11079250663245688 1104 16-17 0.17364147877578517 1104 18-19 0.15091334838884052 1104 20-21 0.0587602148693378 1104 22-23 0.04506317921218539 1104 24-25 0.09038812128794405 1104 26-27 0.0749410017786758 1104 28-29 0.03664969021642861 1104 30-31 0.18946533067489213 1104 32-33 0.17441279589562697 1104 34-35 0.21371712436092594 1104 36-37 0.16881791064660945 1104 38-39 0.09171510459841414 1104 40-41 0.131328814767258 1104 42-43 0.023263827473570586 1104 44-45 -0.07760227297906397 1104 46-47 -0.05270093381611929 1104 48-49 0.040857762130293906 1104 50-51 -0.06337406686397173 1104 52-53 0.018703878075228886 1104 54-55 -0.14692451591876932 1104 56-57 0.5475227686817945 1104 58-59 -6.705942086756526 1104 60-61 -9.050833714670105 1104 62-63 -2.860321113504966 1104 64-65 -0.7191373779648629 1104 66-67 -2.2578250573032896 1104 68-69 -4.502590964452722 1104 70-71 -5.003243046605492 1104 72-73 -7.986803325861061 1104 74-75 -8.72402641734935 1104 76-77 -9.22201820383886 1104 78-79 -12.477716017865554 1104 80-81 -8.970119984253774 1104 82-83 -5.316975981124202 1104 84-85 -4.320362338771432 1104 86-87 -4.080517887740751 1104 88-89 -4.412554544477306 1104 90-91 -9.579470742729733 1104 92-93 -15.10960492715384 1104 94-95 -12.848855741037152 1104 96-97 -12.387935244155333 1104 98-99 -11.747332777346159 1104 100-101 -11.390231731080313 1105 1 0.13476604130458014 1105 2 0.213976696019607 1105 3 0.11355487758579486 1105 4 0.07703016245066152 1105 5 0.08721281610370824 1105 6 0.17758054573309323 1105 7 0.17610971421810007 1105 8 0.22017749669309694 1105 9 0.12253653055660152 1105 10-11 0.20607454433410055 1105 12-13 0.2689586415699381 1105 14-15 0.2560023010173751 1105 16-17 0.19787920754531285 1105 18-19 0.25418770672824564 1105 20-21 0.2089410907509759 1105 22-23 0.19099012603211207 1105 24-25 0.20100039456199426 1105 26-27 0.2802668828974575 1105 28-29 0.17529662322022688 1105 30-31 0.3927001599807909 1105 32-33 0.21311698889828534 1105 34-35 0.24566702166157484 1105 36-37 0.213162053257264 1105 38-39 0.253920480066121 1105 40-41 0.29678446651747237 1105 42-43 0.382442497102204 1105 44-45 0.467877452814065 1105 46-47 0.42951500810485044 1105 48-49 0.33675561814603583 1105 50-51 0.25328464591201794 1105 52-53 0.2823105407509203 1105 54-55 0.3182220159845457 1105 56-57 1.0428752522591154 1105 58-59 -6.4503109525437345 1105 60-61 -8.181435597798796 1105 62-63 -2.1861305070298194 1105 64-65 0.05551617700353262 1105 66-67 -1.2659161876533354 1105 68-69 -3.631517722142352 1105 70-71 -4.674066172111877 1105 72-73 -5.749635736382796 1105 74-75 -6.123325954060263 1105 76-77 -8.13973129798648 1105 78-79 -11.680005596385481 1105 80-81 -7.620504515799663 1105 82-83 -3.5212450165677005 1105 84-85 -2.5872508328374693 1105 86-87 -2.3442537760961777 1105 88-89 -2.4393970221803656 1105 90-91 -7.907601189742394 1105 92-93 -11.335767297116744 1105 94-95 -11.607068984645789 1105 96-97 -11.981916372798395 1105 98-99 -11.586450512558935 1105 100-101 -11.185281706357138 1106 1 0.13010575858683993 1106 2 0.1190564953812121 1106 3 0.11061826107872719 1106 4 0.09961110180314847 1106 5 0.09035919093270905 1106 6 0.10144739479829923 1106 7 0.1333828560329664 1106 8 0.19868182865843664 1106 9 0.1881452856865664 1106 10-11 0.189152519710305 1106 12-13 0.2533134067318201 1106 14-15 0.2830428846926978 1106 16-17 0.18103470230135343 1106 18-19 0.28513617914046563 1106 20-21 0.18462280529725206 1106 22-23 0.2963982427671823 1106 24-25 0.26691466724781066 1106 26-27 0.3561583557656789 1106 28-29 0.23798700169719922 1106 30-31 0.337579320947512 1106 32-33 0.27647312204921803 1106 34-35 0.451006738943839 1106 36-37 0.39710642170231836 1106 38-39 0.2723974522503525 1106 40-41 0.342370421829834 1106 42-43 0.24676078255592415 1106 44-45 0.2895418394989733 1106 46-47 0.09490032502231571 1106 48-49 0.24510491135169588 1106 50-51 0.2716749787893562 1106 52-53 0.07754537887222313 1106 54-55 -0.02076111215042431 1106 56-57 0.7467147415431938 1106 58-59 -6.719677117614413 1106 60-61 -9.059091776549366 1106 62-63 -2.5763676251328747 1106 64-65 -0.22757547826322622 1106 66-67 -1.6998240763902253 1106 68-69 -4.144276500072127 1106 70-71 -4.705434161167965 1106 72-73 -6.344779375233685 1106 74-75 -6.760384777589675 1106 76-77 -8.5250618953417 1106 78-79 -11.855080835783564 1106 80-81 -8.074070407272533 1106 82-83 -4.3259964985558454 1106 84-85 -3.2785276226231517 1106 86-87 -3.114919530770141 1106 88-89 -3.2738885862478533 1106 90-91 -8.454003378524884 1106 92-93 -11.696916407923858 1106 94-95 -11.901492149260472 1106 96-97 -12.191665574804778 1106 98-99 -11.69607659555027 1106 100-101 -11.419517912467487 1107 1 0.16379717307226116 1107 2 0.12151246502215685 1107 3 0.14419312620365332 1107 4 0.11794200251996045 1107 5 0.13020112491690838 1107 6 0.14546110394385892 1107 7 0.10721547039297263 1107 8 0.11160648241602189 1107 9 0.11567623397453985 1107 10-11 0.12698143649319604 1107 12-13 0.1667318275852807 1107 14-15 0.23084109644926798 1107 16-17 0.2625699536176711 1107 18-19 0.21298077278538585 1107 20-21 0.06699584974386141 1107 22-23 0.19436176433097074 1107 24-25 0.22819227744534487 1107 26-27 0.29289486555560273 1107 28-29 0.3896689668188813 1107 30-31 0.22270987136754172 1107 32-33 0.35503647278927275 1107 34-35 0.20726602343022904 1107 36-37 0.2347655959100976 1107 38-39 0.26754281460209484 1107 40-41 0.2101715527262229 1107 42-43 0.3994539593368742 1107 44-45 0.34260088180487713 1107 46-47 0.13052258832872354 1107 48-49 0.26406299466611216 1107 50-51 0.37489543871605235 1107 52-53 0.2790909012360743 1107 54-55 0.30835990241283895 1107 56-57 0.9035101747111156 1107 58-59 -6.5664307097781425 1107 60-61 -8.928717586070086 1107 62-63 -2.537680481240905 1107 64-65 -0.07590108128183459 1107 66-67 -1.422559202291648 1107 68-69 -3.8495354926445398 1107 70-71 -4.570042401791579 1107 72-73 -6.045663225903466 1107 74-75 -6.5992258790784355 1107 76-77 -8.735915365334026 1107 78-79 -11.979063839902796 1107 80-81 -7.850311095757068 1107 82-83 -3.7895902840315046 1107 84-85 -2.8781517022832297 1107 86-87 -2.681258929427333 1107 88-89 -2.705358643441368 1107 90-91 -8.07629630880069 1107 92-93 -11.449081436628733 1107 94-95 -11.884878243343268 1107 96-97 -12.418823521892666 1107 98-99 -11.682379234668907 1107 100-101 -11.204596999533535 1108 1 0.08754083131999835 1108 2 -0.031920207034175974 1108 3 0.015379907260363979 1108 4 -0.013504258312138973 1108 5 -0.09648409600038121 1108 6 0.07983024376604675 1108 7 0.07185363223621977 1108 8 0.010972635980834866 1108 9 0.030160719944767322 1108 10-11 0.14123604014137214 1108 12-13 -0.0267706567178152 1108 14-15 -0.06564395024009428 1108 16-17 0.041133072201418486 1108 18-19 0.0745912809042295 1108 20-21 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2.065981811860123 2312 84-85 1.7041132570579904 2312 86-87 1.4238226245782677 2312 88-89 1.764069481996568 2312 90-91 2.9899556186559018 2312 92-93 5.055419434977878 2312 94-95 8.87682161520015 2312 96-97 9.576169895442252 2312 98-99 10.046324272897214 2312 100-101 8.987999343065622 2313 1 -0.30368790050208716 2313 2 -0.27137378072714213 2313 3 -0.32059591887075456 2313 4 -0.26088362862103054 2313 5 -0.3814572884680345 2313 6 -0.3552140458427573 2313 7 -0.26455504136273333 2313 8 -0.30965760170477097 2313 9 -0.5326725873210165 2313 10-11 -0.40203661886647524 2313 12-13 -0.43520828127445554 2313 14-15 -0.47304108011872614 2313 16-17 -0.43383072753925234 2313 18-19 -0.5122009430614369 2313 20-21 -0.5325035338718038 2313 22-23 -0.4586455251972765 2313 24-25 -0.31637838213813296 2313 26-27 -0.3668675130610808 2313 28-29 -0.5374112650043017 2313 30-31 -0.542252792979852 2313 32-33 -0.3575890241053017 2313 34-35 -0.4499727053554423 2313 36-37 -0.5435622918243723 2313 38-39 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3.7359067219992284 2314 62-63 1.191328814759018 2314 64-65 1.0434211518125132 2314 66-67 1.7115064319900988 2314 68-69 -0.5090715797602705 2314 70-71 2.434722323433819 2314 72-73 8.417042052146115 2314 74-75 9.978967373934577 2314 76-77 8.725393878573083 2314 78-79 6.448151622259211 2314 80-81 3.4990666251921816 2314 82-83 1.953641818483959 2314 84-85 1.414295106014535 2314 86-87 1.3621568653016176 2314 88-89 1.4534827616106334 2314 90-91 2.6961991505865903 2314 92-93 4.719397139616543 2314 94-95 8.580834877976969 2314 96-97 9.249218168369179 2314 98-99 9.536195823343549 2314 100-101 8.872257247965377 2315 1 -0.7498130455339691 2315 2 -0.6405342398404343 2315 3 -0.5361435252306173 2315 4 -0.3792995592627264 2315 5 -0.7141816768775797 2315 6 -0.7210279061661922 2315 7 -0.6385365112962518 2315 8 -0.7689045886320613 2315 9 -1.0969221819876225 2315 10-11 -0.9361552502190591 2315 12-13 -0.6693343768084219 2315 14-15 -0.9517927288524604 2315 16-17 -0.9686780614092498 2315 18-19 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1.0446078055325785 2315 86-87 1.0994779535656285 2315 88-89 1.1569749999817027 2315 90-91 2.6329046073010396 2315 92-93 4.561596252531871 2315 94-95 8.571649145649534 2315 96-97 9.158542812307132 2315 98-99 9.191517800526045 2315 100-101 8.368806777181211 2316 1 -0.590823039902606 2316 2 -0.5447098470265885 2316 3 -0.6472363721582077 2316 4 -0.43516410792940263 2316 5 -0.762569894370472 2316 6 -0.7821043153286169 2316 7 -0.7143286093462251 2316 8 -0.8326592645799238 2316 9 -0.72927004450775 2316 10-11 -0.8471167505877446 2316 12-13 -0.8436325049773572 2316 14-15 -0.9886151377265691 2316 16-17 -1.0869480410720556 2316 18-19 -1.181163445611567 2316 20-21 -0.9964262159508621 2316 22-23 -1.1108397409322421 2316 24-25 -2.329416507015438 2316 26-27 -1.8289533820865742 2316 28-29 -1.3825952301917042 2316 30-31 -0.9560106310111962 2316 32-33 -0.9089906796668785 2316 34-35 -1.1312641088297752 2316 36-37 -1.0002195182102014 2316 38-39 -1.2151490952292718 2316 40-41 -1.0233065780984631 2316 42-43 -1.2503495690299147 2316 44-45 -0.9977243475513902 2316 46-47 -0.9578702956904195 2316 48-49 -0.9092541794487587 2316 50-51 -1.1856994804472407 2316 52-53 -1.289865410104575 2316 54-55 -1.1662480979770464 2316 56-57 0.011122112656273941 2316 58-59 3.7193584711902865 2316 60-61 3.579029354452807 2316 62-63 1.0435843335659492 2316 64-65 0.6900339292273898 2316 66-67 5.81999307985156 2316 68-69 8.592859664750165 2316 70-71 5.747060401321578 2316 72-73 9.319217135199164 2316 74-75 10.156023597371487 2316 76-77 8.726143678405283 2316 78-79 6.224949040647516 2316 80-81 3.0965457642420837 2316 82-83 1.7450761848256882 2316 84-85 1.5287409545416963 2316 86-87 1.411798603028469 2316 88-89 1.37245621166797 2316 90-91 2.775506887016835 2316 92-93 4.575192151076116 2316 94-95 8.71220344575853 2316 96-97 9.181312746856825 2316 98-99 9.654665056974887 2316 100-101 8.772077010929342 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 36.0 5 87.0 6 202.0 7 540.0 8 1159.0 9 2042.0 10 3227.0 11 5001.0 12 6930.0 13 9544.0 14 12652.0 15 17906.0 16 24210.0 17 31473.0 18 35509.0 19 36864.0 20 38756.0 21 39088.0 22 37708.0 23 35994.0 24 36036.0 25 38461.0 26 50529.0 27 74227.0 28 105320.0 29 137228.0 30 160731.0 31 168793.0 32 172477.0 33 175939.0 34 176027.0 35 167666.0 36 135862.0 37 80176.0 38 24134.0 39 975.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.86364559650922 19.37256955746062 14.552382537278003 23.211402308752163 2 15.593762631971991 21.20982115329843 39.04452620256373 24.151890012165843 3 20.266492189384845 24.614069335144663 29.745326134351096 25.3741123411194 4 13.115131941131134 16.815649429294968 36.989907145740816 33.07931148383309 5 13.397078813662237 38.234018090436514 34.10510512275094 14.263797973150313 6 32.014337969929414 36.34915158855409 17.15168642419349 14.484824017323009 7 28.541759949107547 32.746797812603226 20.38360186442544 18.327840373863786 8 26.08100344601809 34.89883622263176 19.402422684801408 19.617737646548747 9 26.143061621462916 16.997345273485767 20.140541466338803 36.71905163871252 10-11 25.27852607626528 26.270121481263992 27.192473789163333 21.2588786533074 12-13 26.64065768708482 23.600102763607005 27.418798399823835 22.34044114948435 14-15 22.8984301597244 25.24029615565299 26.281587652677736 25.57968603194488 16-17 22.17486940458277 28.53429735383712 26.75404020014436 22.536793041435754 18-19 21.949314009004635 27.728946879709284 28.480988655023104 21.84075045626298 20-21 23.117701031306197 26.25108574644303 28.269950208585655 22.361263013665113 22-23 22.50775008869478 26.375821191323816 28.285731762518196 22.830696957463207 24-25 22.83957866921129 26.406430065695307 28.416339413513413 22.33765185157999 26-27 22.55769143721197 26.373162027822055 28.821263284353364 22.247883250612606 28-29 22.078015819069698 27.311286661058592 28.541612531153916 22.06908498871779 30-31 22.415846032325536 26.621611304639757 28.243222609279524 22.71932005375518 32-33 22.228453299098298 26.704013979796144 28.365803034518127 22.701729686587434 34-35 23.013779273248304 26.496596042875375 28.241564489230264 22.248060194646058 36-37 22.911440362495235 26.173021281469794 28.61632511009809 22.299213245936883 38-39 22.478709269094722 26.60885530917908 28.66806675488805 22.24436866683815 40-41 22.50949557851991 26.665963426450663 28.55636613100484 22.268174864024587 42-43 22.16091512910794 26.774154230523916 28.016968989717085 23.047961650651057 44-45 22.396620870500804 26.47617863490648 28.139359232226063 22.987841262366654 46-47 22.7778724162474 26.864198364843052 27.723287231345044 22.634641987564503 48-49 22.64624061788218 26.782767595647062 27.803670266377985 22.76732152009277 50-51 22.14779046089987 27.023607902259467 28.183150918275444 22.64545071856522 52-53 22.528261861269208 27.178253344532354 27.565275403859747 22.72820939033869 54-55 21.99927661569695 26.70213109603 27.915170220451124 23.383422067821925 56-57 22.508385705710122 26.72319208105161 27.73724380413344 23.03117840910483 58-59 21.196709780348773 26.38719079610288 29.392697917491056 23.023401506057294 60-61 22.362426015899434 26.557660112170673 28.421158802433066 22.658755069496834 62-63 22.155594268712807 26.658828883495143 28.16412366896336 23.021453178828686 64-65 21.22409422442871 27.06479828567018 28.501272392453124 23.209835097447986 66-67 21.437833815999742 26.411287370453767 28.750447032066933 23.400431781479558 68-69 22.182156961108618 25.884941454846743 29.07815114745483 22.85475043658981 70-71 21.34499113709108 26.172431413440755 29.252176583861978 23.230400865606185 72-73 21.165807145679768 25.9974744169406 29.437677560580962 23.39904087679867 74-75 21.537480422121675 26.699777367649567 28.516524947100585 23.246217263128177 76-77 20.142745384452095 26.654662357782833 29.132814861987562 24.06977739577751 78-79 21.077286594955023 26.82738881549978 28.172080423979367 23.92324416556583 80-81 21.95199411995133 26.938095311497978 27.308607865690604 23.801302702860088 82-83 21.95608020570383 26.69111956547536 27.39292314415246 23.959877084668346 84-85 21.768449991289557 26.801324574607392 27.201050733928255 24.229174700174795 86-87 21.827417005102596 26.882350551929097 27.20197859620087 24.088253846767433 88-89 21.763575626325988 26.71203599467489 26.92040149261729 24.603986886381836 90-91 21.500526874524407 26.506877079695258 27.299138436925013 24.69345760885532 92-93 21.451485211611097 26.69216572015422 27.458632463130133 24.39771660510455 94-95 20.036532274596386 26.543530740974575 28.579898920267777 24.840038064161263 96-97 19.523057815372404 26.429757060084235 29.008959705984626 25.038225418558728 98-99 20.00642151221422 26.71596252486544 28.002143470127855 25.275472492792485 100-101 19.735406189578885 26.520972191853854 28.22132522927778 25.52229638928948 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 368.0 1 259.5 2 154.5 3 182.5 4 218.5 5 251.0 6 359.5 7 569.0 8 774.5 9 926.5 10 1037.0 11 1161.0 12 1318.5 13 1515.0 14 1684.0 15 1817.0 16 1942.5 17 2122.0 18 2397.0 19 2634.5 20 2990.5 21 3523.0 22 4089.5 23 4769.5 24 5819.5 25 7251.5 26 9082.0 27 11329.5 28 13868.0 29 16842.0 30 20343.5 31 24507.0 32 28955.5 33 33257.5 34 37457.0 35 41702.5 36 46145.5 37 50523.5 38 54688.0 39 58277.0 40 61409.5 41 64600.5 42 68022.5 43 71680.5 44 75957.0 45 80388.5 46 85386.0 47 91588.5 48 96211.0 49 94346.5 50 87584.5 51 81600.5 52 76737.5 53 72377.0 54 67918.5 55 62611.0 56 56127.5 57 49261.5 58 42693.5 59 35919.0 60 29438.0 61 23536.5 62 18358.5 63 14025.5 64 10485.0 65 7778.5 66 5729.0 67 4283.5 68 3132.0 69 2194.5 70 1605.5 71 1158.5 72 787.5 73 581.0 74 416.0 75 259.0 76 176.5 77 102.0 78 50.5 79 30.5 80 14.5 81 12.5 82 8.5 83 3.0 84 3.0 85 2.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021042071910057376 2 0.004844570044408559 3 0.004306284483918719 4 0.0 5 0.06562190332880684 6 0.0 7 0.0 8 3.4254535667535264E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.4467525476810903E-4 16-17 0.0 18-19 0.0010521035955028688 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.2233762738405452E-4 28-29 0.0020552721400521157 30-31 0.009150854528327278 32-33 2.202077292912981E-4 34-35 0.0010276360700260578 36-37 0.028088719247378914 38-39 0.02733022595759778 40-41 0.029385498097649895 42-43 0.03043760169315276 44-45 0.03092695220268898 46-47 0.03244393878225126 48-49 0.033080094444648336 50-51 0.027648303788796322 52-53 0.023929239916321062 54-55 0.017176202884721253 56-57 0.13946489521782215 58-59 0.7592028480199655 60-61 0.29821020051137126 62-63 2.4467525476810903E-4 64-65 0.1505976193097711 66-67 1.4117028174355586 68-69 2.6964436451719456 70-71 2.175823638076363 72-73 1.8618563511579256 74-75 1.550653894618368 76-77 1.105149190736595 78-79 0.42617535875509227 80-81 2.202077292912981E-4 82-83 2.202077292912981E-4 84-85 4.8935050953621806E-5 86-87 0.002740362853402821 88-89 2.4467525476810903E-5 90-91 0.2258107926254878 92-93 0.17518748241396606 94-95 1.292105552904907 96-97 1.2828568282746724 98-99 1.0098726465298933 100-101 0.40762897444366963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2043525.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.5270540768692 #Duplication Level Percentage of deduplicated Percentage of total 1 74.67362389615872 40.71732728301639 2 13.944390002438789 15.206930154638684 3 4.899005248449571 8.013849723152275 4 2.2985295173739657 5.013281731645212 5 1.1921412468468844 3.250197511704317 6 0.7136095473801543 2.3346615827860693 7 0.4778767826245467 1.8240049217774248 8 0.3248312382014343 1.416967239301279 9 0.23720132945416694 1.1640500746427367 >10 1.1322126117917615 11.098490526732744 >50 0.0702414701902105 2.5810624596831464 >100 0.03148238275441027 3.1272338743357277 >500 0.0027869725259392635 1.0837584191272183 >1k 0.0017081444513821187 1.9016264064946113 >5k 3.596093581857092E-4 1.2665580909620868 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9284 0.45431301305342486 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6085 0.29776978505278867 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5272 0.25798558862749416 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5196 0.25426652475501893 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3808 0.18634467403139185 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3782 0.18507236270659766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3569 0.1746491968534762 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2603 0.12737793763227756 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2497 0.12219082223119365 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2361 0.11553565530150109 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2203 0.10780391725082883 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2130 0.10423165853121444 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2119 0.1036933729707246 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 4.8935050953621806E-5 8 0.0 0.0 0.0 0.0 4.8935050953621806E-5 9 0.0 0.0 0.0 0.0 4.8935050953621806E-5 10-11 0.0 0.0 0.0 2.4467525476810903E-5 4.8935050953621806E-5 12-13 0.0 0.0 0.0 1.2233762738405452E-4 4.8935050953621806E-5 14-15 0.0 0.0 0.0 1.9574020381448723E-4 7.340257643043271E-5 16-17 0.0 0.0 0.0 1.9574020381448723E-4 9.787010190724361E-5 18-19 0.0 0.0 0.0 2.2020772929129813E-4 9.787010190724361E-5 20-21 0.0 0.0 0.0 2.9361030572173084E-4 9.787010190724361E-5 22-23 0.0 0.0 0.0 5.13818035013029E-4 1.2233762738405452E-4 24-25 0.0 0.0 0.0 9.542334935956252E-4 1.4680515286086542E-4 26-27 0.0 0.0 0.0 0.0019574020381448723 1.4680515286086542E-4 28-29 0.0 0.0 0.0 0.0067530370315998095 1.4680515286086542E-4 30-31 0.0 0.0 0.0 0.0285046671804847 1.4680515286086542E-4 32-33 0.0 0.0 0.0 0.07440574497498195 1.4680515286086542E-4 34-35 0.0 0.0 0.0 0.1447498807208133 1.4680515286086542E-4 36-37 0.0 0.0 0.0 0.25502501804480004 1.4680515286086542E-4 38-39 0.0 0.0 0.0 0.4195446593508766 1.4680515286086542E-4 40-41 0.0 0.0 0.0 0.6030755679524351 1.4680515286086542E-4 42-43 0.0 0.0 0.0 0.7855543729584908 1.4680515286086542E-4 44-45 0.0 0.0 0.0 1.0016515579696847 1.4680515286086542E-4 46-47 0.0 0.0 0.0 1.2564808358106703 1.7127267833767632E-4 48-49 0.0 0.0 0.0 1.4895095484518173 1.9574020381448723E-4 50-51 0.0 0.0 0.0 1.7260126497106716 1.9574020381448723E-4 52-53 4.8935050953621806E-5 0.0 0.0 1.9459023011707712 1.9574020381448723E-4 54-55 4.8935050953621806E-5 0.0 0.0 2.191751997161767 1.9574020381448723E-4 56-57 4.8935050953621806E-5 0.0 0.0 2.4115437785199596 1.9574020381448723E-4 58-59 4.8935050953621806E-5 0.0 0.0 2.563071163797849 1.9574020381448723E-4 60-61 4.8935050953621806E-5 2.4467525476810903E-5 0.0 2.675034560379736 1.9574020381448723E-4 62-63 4.8935050953621806E-5 4.8935050953621806E-5 0.0 2.7688675205833055 1.9574020381448723E-4 64-65 4.8935050953621806E-5 4.8935050953621806E-5 0.0 2.8624802730575842 1.9574020381448723E-4 66-67 4.8935050953621806E-5 4.8935050953621806E-5 0.0 2.9377668489497317 1.9574020381448723E-4 68-69 4.8935050953621806E-5 4.8935050953621806E-5 0.0 3.0249935772745626 1.9574020381448723E-4 70-71 9.787010190724361E-5 4.8935050953621806E-5 0.0 3.1889504654946723 1.9574020381448723E-4 72-73 9.787010190724361E-5 4.8935050953621806E-5 0.0 3.39949352222263 2.4467525476810903E-4 74-75 9.787010190724361E-5 4.8935050953621806E-5 0.0 3.6464197893346055 2.4467525476810903E-4 76-77 9.787010190724361E-5 4.8935050953621806E-5 0.0 3.993002287713632 2.4467525476810903E-4 78-79 9.787010190724361E-5 4.8935050953621806E-5 0.0 4.5199349163822315 2.4467525476810903E-4 80-81 9.787010190724361E-5 4.8935050953621806E-5 0.0 5.108427839150488 3.1807783119854174E-4 82-83 9.787010190724361E-5 4.8935050953621806E-5 0.0 5.6437283615321565 3.9148040762897445E-4 84-85 9.787010190724361E-5 4.8935050953621806E-5 0.0 6.078589691831517 3.9148040762897445E-4 86-87 9.787010190724361E-5 4.8935050953621806E-5 0.0 6.458007609400424 3.9148040762897445E-4 88-89 9.787010190724361E-5 4.8935050953621806E-5 0.0 6.844985992341664 3.9148040762897445E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9085 0.0 25.855844 1 TACATGG 9135 0.0 25.138332 2 ACATGGG 9380 0.0 23.526594 3 ATGGGAG 2285 0.0 20.841547 5 TACCTGG 1520 0.0 20.784866 2 GTAAGGT 780 0.0 20.553185 4 CATGGGA 5920 0.0 20.15086 4 GAGTACT 5765 0.0 19.533966 12-13 CATGGGG 3745 0.0 18.88575 4 GTATAGG 595 0.0 18.233295 1 AGTACTT 6375 0.0 18.182213 12-13 GTACTTT 6340 0.0 18.02247 14-15 TAGCCCT 1205 0.0 17.999712 4 GGACCGC 210 2.0982323E-5 17.94926 6 GTGTAGC 1265 0.0 17.525148 1 CGCGGAA 230 0.0 17.413586 82-83 TACGCGG 140 5.5298733E-7 17.38422 64-65 CGCATAG 435 0.0 17.345133 66-67 ACAACCG 435 5.456968E-12 17.34305 5 GTACCTG 1960 0.0 16.605324 1 >>END_MODULE