FastQCFastQC Report
Fri 27 May 2016
SRR939812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128867
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86670.40711796462625427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58310.27390156360167167No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47610.22364008648731928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44010.20672968297221012No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33780.15867595298344142No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33070.1553408456235171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.1400745091168213No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25440.11950018484010508No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23610.1109040630532579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95500.027.387391
TACATGG96650.026.5754262
ACATGGG101250.024.8581393
CATGGGA61050.021.4989974
GAGTACT57400.020.65543212-13
CATGGGG41600.020.1291924
AGTACTT60950.019.9927112-13
GTACTTT60800.019.73482514-15
ACCGTAT1250.00637529418.8191748
TACCTGG16150.018.6421892
ATGGGAG24100.017.7637315
GTGTAGC12250.017.6644861
TAAGGTG8950.017.3460585
ACTTTTT77650.016.75798816-17
TACTTTT73650.016.57911514-15
CCGTACG1202.857953E-416.5510194-95
AGAGTAC132500.016.33381310-11
AGTACAT70450.016.2928962
ATGGGAT27250.016.0555935
TAGCCCT13150.015.7407924
Produced by FastQC (version 0.11.5)