FastQCFastQC Report
Fri 27 May 2016
SRR939812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128867
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT97920.45996297561097055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62400.2931136609285596No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55530.2608429742205596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51900.2437916506761578No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39930.18756455898841967No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38440.18056553086688834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.17215730245243127No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25830.12133214522090857No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25410.11935926481081252No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24180.11358154360981687No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23050.10827355583979649No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22420.10531423522465236No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21510.10103966100277753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG101150.025.426941
TACATGG101000.025.130512
ACATGGG101400.024.090533
CGCATAG2050.021.01160466-67
CATGGGA63100.020.8974114
GAGTACT60000.020.3169712-13
TACCTGG14750.020.2636382
CATGGGG41900.019.4711464
GTACTTT63200.018.98805814-15
AGTACTT64550.018.9583512-13
GGACCGT2704.951362E-717.5676356
ATGGGAG23550.016.918645
ATGGGAT27000.016.6892535
GCACCGT4055.2568794E-1016.396466
ACTTTTT75150.016.37892716-17
GTGTAGG7600.016.2338121
TACACCG2053.3699555E-416.1965035
ATGGGGA24100.015.9420825
TACTTTT74550.015.93812414-15
ACGCATA2900.015.67819964-65
Produced by FastQC (version 0.11.5)