FastQCFastQC Report
Fri 27 May 2016
SRR939811_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939811_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867292
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127440.4444611849787186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90490.3155939471808243No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76110.2654420965845125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64360.22446266372591284No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44260.154361676452904No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34240.11941581115561303No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31500.1098597561741183No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29300.1021870112984656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG149450.027.97111
TACATGG156600.027.2999972
ACATGGG157350.025.5725383
CATGGGA117000.022.9954534
GAGTACT109600.022.36070812-13
TCGCGTA1754.0904506E-621.6910979
AGTACTT115500.021.1568512-13
CATGGGG40900.021.1150844
GTACTTT117550.020.82952714-15
ATGGGAG44300.020.3514185
GTATCAA279850.020.3463021
TACCGTA1250.006069414318.9803717
ATGGGAT49050.018.6708055
GTATAGG8200.018.5167881
TATCAAC320450.018.5088422
ATCAACG323350.018.2260883
TCAACGC323600.018.182684
CAACGCA323900.018.107245
AACGCAG326550.017.9748296
ACTTTTT144350.017.96776216-17
Produced by FastQC (version 0.11.5)