FastQCFastQC Report
Fri 27 May 2016
SRR939811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867292
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142900.49837965578671445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113330.39525098943532777No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93100.3246966126923941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85390.2978071295145384No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51260.17877495560270806No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38800.13531931871605682No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35150.12258953744508755No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32800.11439365087336761No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG156750.024.0333211
TACATGG158550.023.6060162
ACATGGG159250.022.7326563
GAGTACT119250.020.93529712-13
AGTACTT122050.020.62868512-13
GTATCAA273650.020.3828011
CATGGGA117850.020.0386754
GTACTTT125700.019.82357614-15
GGTATCA210950.019.3111781
CATGGGG42950.018.6572074
CTCGCGA951.5713314E-417.89925670-71
ATCAACG311500.017.674753
CAACGCA312450.017.6357885
TCAACGC312200.017.6348114
AACGCAG313700.017.5829856
ACTTTTT146550.017.37271716-17
TATCAAC319700.017.295132
AGAGTAC242550.017.26467110-11
CGCGCGT2756.4568485E-717.1284317
TACTTTT147700.017.06219514-15
Produced by FastQC (version 0.11.5)