##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939811_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2867292 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.395995245688265 31.0 31.0 34.0 30.0 34.0 2 31.590570475556728 31.0 31.0 34.0 30.0 34.0 3 31.651004850569805 31.0 31.0 34.0 30.0 34.0 4 35.46254061323367 37.0 35.0 37.0 33.0 37.0 5 35.19952415031326 37.0 35.0 37.0 32.0 37.0 6 35.23325318802549 37.0 35.0 37.0 32.0 37.0 7 35.15436934919778 37.0 35.0 37.0 32.0 37.0 8 35.097643700048685 37.0 35.0 37.0 32.0 37.0 9 36.679979576548185 39.0 35.0 39.0 32.0 39.0 10-11 36.53605562321522 38.0 35.0 39.0 32.0 39.0 12-13 36.48520415779069 38.0 35.0 39.0 32.0 39.0 14-15 37.46808556645085 39.0 36.0 41.0 32.0 41.0 16-17 37.417789851888124 39.0 36.0 41.0 32.0 41.0 18-19 37.4401133543427 39.0 36.0 41.0 32.0 41.0 20-21 37.38674784430745 39.0 36.0 41.0 32.0 41.0 22-23 37.24102358601775 39.0 36.0 40.0 31.0 41.0 24-25 37.083485741947456 39.0 36.0 40.0 31.0 41.0 26-27 36.78311277679427 39.0 35.5 40.0 30.5 41.0 28-29 36.56784118952656 38.0 35.0 40.0 30.0 41.0 30-31 36.32236793462263 38.0 35.0 40.0 30.0 41.0 32-33 36.2644727150217 38.0 35.0 40.0 30.0 41.0 34-35 36.309680527829045 38.0 35.0 40.0 30.0 41.0 36-37 36.26001641967403 38.0 35.0 40.0 30.0 41.0 38-39 36.11987530394532 38.0 35.0 40.0 30.0 41.0 40-41 35.960401661219024 38.0 35.0 40.0 29.5 41.0 42-43 35.74636451397346 38.0 35.0 40.0 28.5 41.0 44-45 35.47245850788828 38.0 34.0 40.0 27.5 41.0 46-47 35.13578805367573 38.0 34.0 40.0 26.5 41.0 48-49 34.81245038872916 38.0 34.0 40.0 25.5 41.0 50-51 34.54614650339066 38.0 33.0 40.0 24.0 41.0 52-53 34.28673256856993 38.0 33.0 40.0 24.0 41.0 54-55 34.01081299009658 38.0 33.0 40.0 23.5 41.0 56-57 32.77950850488894 36.5 31.5 40.0 16.0 41.0 58-59 29.61454832643484 35.0 24.5 39.0 7.0 40.0 60-61 29.61897672089205 34.5 25.0 39.0 8.0 40.0 62-63 31.387939561091095 35.0 29.5 39.0 8.0 40.0 64-65 30.929742418979302 35.0 29.0 39.0 4.0 40.0 66-67 25.50022477654874 28.5 18.0 36.0 2.0 39.0 68-69 17.80324623372855 13.5 7.0 29.5 2.0 35.5 70-71 16.65872084182567 15.0 7.5 26.0 2.0 33.5 72-73 18.86260555255621 19.5 7.5 30.0 2.0 35.0 74-75 19.592553531345953 20.0 8.5 31.5 2.0 35.5 76-77 20.59491848057331 23.5 9.5 31.5 2.0 35.0 78-79 23.735251066162775 29.0 12.0 34.0 2.0 36.0 80-81 26.28869051355774 31.5 23.0 35.0 2.0 36.0 82-83 27.13944307032559 33.0 26.0 35.0 2.0 36.0 84-85 27.072032949556586 33.0 26.0 35.0 2.0 36.0 86-87 26.762919332945508 33.0 25.5 35.0 2.0 36.0 88-89 26.299979388217174 33.0 24.5 35.0 2.0 35.0 90-91 24.75132023526031 31.5 11.5 34.5 2.0 35.0 92-93 22.75905087448366 31.0 2.0 34.0 2.0 35.0 94-95 18.340144812596694 24.0 2.0 33.0 2.0 35.0 96-97 17.023633274880968 15.5 2.0 32.5 2.0 34.5 98-99 15.918971628979538 6.0 2.0 32.0 2.0 34.0 100-101 15.283597031624264 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4790150705023528 1101 2 0.35887154262927723 1101 3 0.4383852494758038 1101 4 0.3840412972341909 1101 5 0.4316539499508636 1101 6 0.5309141476429033 1101 7 0.530314799861543 1101 8 0.49787787912351433 1101 9 0.5143285899216892 1101 10-11 0.556379282433646 1101 12-13 0.5436886859153063 1101 14-15 0.49391522014814626 1101 16-17 0.6101429926855957 1101 18-19 0.5818273222250596 1101 20-21 0.7791165927317607 1101 22-23 0.7414194296245284 1101 24-25 0.8313614952222466 1101 26-27 0.6841319785477324 1101 28-29 0.7094932938994205 1101 30-31 0.9518339583441602 1101 32-33 0.5126887728408036 1101 34-35 1.0103038364332235 1101 36-37 0.9721788130682114 1101 38-39 0.8080242583806978 1101 40-41 0.9012415389187112 1101 42-43 1.016973172104791 1101 44-45 0.9490349859452039 1101 46-47 0.8471740340737455 1101 48-49 0.5695101132535001 1101 50-51 0.6793072683535399 1101 52-53 0.8062149497086324 1101 54-55 0.6599492053388971 1101 56-57 1.1226401183292225 1101 58-59 -7.92812394036898 1101 60-61 -9.285356173223104 1101 62-63 -2.076837672688672 1101 64-65 0.4556056246781459 1101 66-67 -0.7228219056771117 1101 68-69 -3.283326107244097 1101 70-71 -4.404291464242654 1101 72-73 -6.7026041992834156 1101 74-75 -7.436186981577606 1101 76-77 -8.073434546567896 1101 78-79 -11.545086429528787 1101 80-81 -6.960844513629912 1101 82-83 -2.9495518356507517 1101 84-85 -2.1859747406423473 1101 86-87 -1.862373388984114 1101 88-89 -1.9800777425370732 1101 90-91 -8.117614972310463 1101 92-93 -14.122549681276155 1101 94-95 -12.103457256987761 1101 96-97 -12.236445576825654 1101 98-99 -11.870841093687872 1101 100-101 -11.592745991482456 1102 1 0.5630669378903015 1102 2 0.6401842578473769 1102 3 0.5628111250800849 1102 4 0.47008720484366506 1102 5 0.6037139275528247 1102 6 0.472708839697745 1102 7 0.5577761408954842 1102 8 0.5751879914767883 1102 9 0.7246970405189188 1102 10-11 0.8043747693117993 1102 12-13 0.8499905791364029 1102 14-15 1.0225715889282867 1102 16-17 0.9838906432649566 1102 18-19 1.0404180329021102 1102 20-21 0.9582020711478165 1102 22-23 0.984216727336964 1102 24-25 0.9276007465355463 1102 26-27 0.9958219695270785 1102 28-29 1.0429926110663175 1102 30-31 1.037837695074991 1102 32-33 0.9058841804041862 1102 34-35 0.7829067291879284 1102 36-37 0.9482771410347652 1102 38-39 0.8202688941611527 1102 40-41 0.9899950403583802 1102 42-43 1.2130423435168396 1102 44-45 0.9423566268671522 1102 46-47 0.8079380810258527 1102 48-49 0.9450110669856215 1102 50-51 0.8035055329241345 1102 52-53 0.8655396012403287 1102 54-55 0.7453491444564477 1102 56-57 1.4265014068590247 1102 58-59 -8.467818466812354 1102 60-61 -9.433830620742704 1102 62-63 -2.385238614411872 1102 64-65 0.36527951252594804 1102 66-67 -1.008577644128298 1102 68-69 -3.775046651164649 1102 70-71 -4.149437484400892 1102 72-73 -6.813835616867275 1102 74-75 -7.84641942410931 1102 76-77 -8.694724137622085 1102 78-79 -11.623796279919915 1102 80-81 -7.218868291302805 1102 82-83 -3.0240306343113375 1102 84-85 -2.0017692707163306 1102 86-87 -1.866910807958778 1102 88-89 -2.079778357226502 1102 90-91 -8.133592708320762 1102 92-93 -14.35576916254642 1102 94-95 -12.512206736833713 1102 96-97 -12.32006484361092 1102 98-99 -11.819472495073647 1102 100-101 -11.691547053853464 1103 1 0.27605200274477326 1103 2 0.2649650875429401 1103 3 0.2504874556644481 1103 4 0.17928781346819278 1103 5 0.1879514513852385 1103 6 0.14835144771442543 1103 7 0.21775595113518875 1103 8 0.36633967755631147 1103 9 0.22225970842082177 1103 10-11 0.2829777278055232 1103 12-13 0.33260901318888614 1103 14-15 0.4663869845488904 1103 16-17 0.4398844905674366 1103 18-19 0.5672422696050603 1103 20-21 0.48531860007262395 1103 22-23 0.47775434273668793 1103 24-25 0.48891810396369095 1103 26-27 0.3263215048498793 1103 28-29 0.48833054524144615 1103 30-31 0.44111897088021834 1103 32-33 0.5548523624755646 1103 34-35 0.5674784688006085 1103 36-37 0.4164637077103279 1103 38-39 0.3038830371566448 1103 40-41 0.4954112904768735 1103 42-43 0.3961916138612551 1103 44-45 0.4780188187558707 1103 46-47 0.34652954718747964 1103 48-49 0.35024467111192337 1103 50-51 0.43190840170731093 1103 52-53 0.3361545094140581 1103 54-55 0.49558382843196824 1103 56-57 0.9905085172508876 1103 58-59 -6.435632998553569 1103 60-61 -8.60823545768547 1103 62-63 -2.489240115451164 1103 64-65 -0.05100901146185066 1103 66-67 -1.4988446356292435 1103 68-69 -4.115223492356449 1103 70-71 -4.877686731193837 1103 72-73 -7.850532802711772 1103 74-75 -8.322121448264706 1103 76-77 -9.182398416004286 1103 78-79 -12.297211271354413 1103 80-81 -8.495579998920679 1103 82-83 -4.723125515435083 1103 84-85 -3.755165909600091 1103 86-87 -3.523069906397051 1103 88-89 -3.6433616390104717 1103 90-91 -9.208083640030315 1103 92-93 -14.734194458967476 1103 94-95 -12.62884218071038 1103 96-97 -12.392875119475752 1103 98-99 -12.022485714187273 1103 100-101 -11.750759242761605 1104 1 0.0836916647297592 1104 2 0.16144887247148887 1104 3 0.12354119461151569 1104 4 0.16214363446010083 1104 5 0.022044895476803106 1104 6 0.1586850985861048 1104 7 0.07385410636057088 1104 8 0.12504834067703996 1104 9 0.09364629073901654 1104 10-11 0.10035778479107194 1104 12-13 0.10054251327000685 1104 14-15 0.2284242313147402 1104 16-17 0.19721349509811148 1104 18-19 0.1591016145764712 1104 20-21 0.13286222946629067 1104 22-23 0.2097961035805156 1104 24-25 0.27383540744734347 1104 26-27 0.2307011243500554 1104 28-29 0.14856382079672414 1104 30-31 0.18489867316525732 1104 32-33 0.18681508679391357 1104 34-35 0.0393253478484894 1104 36-37 0.19613595244938864 1104 38-39 0.11998630084037387 1104 40-41 0.179525216594854 1104 42-43 0.10550694657235482 1104 44-45 0.018207874553198167 1104 46-47 0.04603931724795274 1104 48-49 -0.04139678793661261 1104 50-51 -0.09938117344994879 1104 52-53 0.015452294517167786 1104 54-55 -0.1670881201878629 1104 56-57 0.42928176970564635 1104 58-59 -6.9435873073025824 1104 60-61 -8.978383212078935 1104 62-63 -3.0352980080996446 1104 64-65 -0.7432365509787751 1104 66-67 -2.32006048084617 1104 68-69 -4.3252561511483805 1104 70-71 -4.997608647818872 1104 72-73 -7.937040368210079 1104 74-75 -8.747876324727297 1104 76-77 -9.128622949348756 1104 78-79 -12.197366345729606 1104 80-81 -8.568769320850475 1104 82-83 -4.8803538501365615 1104 84-85 -3.9632421466475485 1104 86-87 -3.7794544351578345 1104 88-89 -4.0327253896256 1104 90-91 -9.363710949613065 1104 92-93 -15.325947404497068 1104 94-95 -12.73820575997261 1104 96-97 -12.341431444512928 1104 98-99 -11.712316026622513 1104 100-101 -11.304296689528417 1105 1 0.17668547752772312 1105 2 0.1692466820215266 1105 3 0.08098874822126945 1105 4 0.10920029151910882 1105 5 0.11221265151205984 1105 6 0.05269157306805283 1105 7 0.1512157965161265 1105 8 0.20003715219293383 1105 9 0.09764595122639719 1105 10-11 0.14969477065500314 1105 12-13 0.21076510771624868 1105 14-15 0.20380444686963983 1105 16-17 0.22616547839788836 1105 18-19 0.20214374263208867 1105 20-21 0.1922105343961107 1105 22-23 0.25911157312884825 1105 24-25 0.32059191932317077 1105 26-27 0.4044037498747528 1105 28-29 0.25078672329724583 1105 30-31 0.1928056965655145 1105 32-33 0.28768223882909183 1105 34-35 0.24254286800556457 1105 36-37 0.03419703860486578 1105 38-39 0.10412432459201426 1105 40-41 0.09010845416821667 1105 42-43 0.10150847319204814 1105 44-45 0.2069348239017117 1105 46-47 0.29761290186019806 1105 48-49 0.143415230691474 1105 50-51 0.12790071343299303 1105 52-53 0.21763139276689714 1105 54-55 0.23219511940358473 1105 56-57 0.7520453490233976 1105 58-59 -6.7197225081136125 1105 60-61 -8.390479491804093 1105 62-63 -2.6373831319861374 1105 64-65 -0.35112462518246446 1105 66-67 -1.821060989521552 1105 68-69 -4.165938666485804 1105 70-71 -4.939169147543463 1105 72-73 -6.355838535079345 1105 74-75 -6.610056161951176 1105 76-77 -8.416774864033744 1105 78-79 -11.755771716676822 1105 80-81 -7.8945393230110135 1105 82-83 -3.8885670729660546 1105 84-85 -2.959500014199641 1105 86-87 -2.8199101536899995 1105 88-89 -2.966018727343318 1105 90-91 -8.420190461925742 1105 92-93 -11.180441242817833 1105 94-95 -11.481850804869 1105 96-97 -11.883725249950828 1105 98-99 -11.249273192238194 1105 100-101 -10.854685155477437 1106 1 0.08183678169835318 1106 2 0.20189552389083687 1106 3 0.11810242163521067 1106 4 0.16161865349438642 1106 5 0.17541674232852245 1106 6 0.19241825762178166 1106 7 0.26375612731357734 1106 8 0.191988186359481 1106 9 0.2874227308759032 1106 10-11 0.28038024562443553 1106 12-13 0.23897671279707566 1106 14-15 0.2750618550003239 1106 16-17 0.18942125631529194 1106 18-19 0.3494980423196239 1106 20-21 0.30559558313269264 1106 22-23 0.3688243966242908 1106 24-25 0.3066753531297124 1106 26-27 0.3762841626923077 1106 28-29 0.36651966124724566 1106 30-31 0.43056933262040076 1106 32-33 0.43716366968088494 1106 34-35 0.4291845649557047 1106 36-37 0.3795505062043816 1106 38-39 0.3170749681866383 1106 40-41 0.4751346904132987 1106 42-43 0.40471328571751286 1106 44-45 0.34198727516512406 1106 46-47 0.16721059154230744 1106 48-49 0.15169546180816695 1106 50-51 0.0982985449096958 1106 52-53 0.20049600440254522 1106 54-55 0.01480182620293391 1106 56-57 0.7325653615896286 1106 58-59 -6.788061770781727 1106 60-61 -8.721633440825965 1106 62-63 -2.5317056170425154 1106 64-65 0.12783617752776522 1106 66-67 -1.4542569504983192 1106 68-69 -4.307177119141691 1106 70-71 -4.952133588516157 1106 72-73 -6.419400933191014 1106 74-75 -6.947557011020596 1106 76-77 -8.843414735484636 1106 78-79 -12.200071489126216 1106 80-81 -8.443300212326324 1106 82-83 -4.305670833494517 1106 84-85 -3.3776956780021123 1106 86-87 -3.1693017954506253 1106 88-89 -3.388702678233141 1106 90-91 -8.436669731895853 1106 92-93 -11.64305469971907 1106 94-95 -12.076314978384826 1106 96-97 -12.486515079797316 1106 98-99 -11.795578678924786 1106 100-101 -11.58446255070227 1107 1 0.1126468065037578 1107 2 0.08805795653132975 1107 3 0.054667942121053414 1107 4 -0.014209975365417904 1107 5 0.02266676371249332 1107 6 0.08607256435345789 1107 7 0.05922914074270835 1107 8 0.06077721210578346 1107 9 0.030468663644697358 1107 10-11 0.19230608601447585 1107 12-13 0.18009381141995107 1107 14-15 0.23311238532279788 1107 16-17 0.2115055519273028 1107 18-19 0.18799613914625013 1107 20-21 0.1580210599952352 1107 22-23 0.17914323162558077 1107 24-25 0.23567736322294763 1107 26-27 0.2774650924673452 1107 28-29 0.11625343081357897 1107 30-31 0.16114039155461768 1107 32-33 0.2634413619227729 1107 34-35 0.06688076288573797 1107 36-37 0.09131169002099426 1107 38-39 0.2031609531323184 1107 40-41 0.13042027334033435 1107 42-43 0.30449730761470306 1107 44-45 0.3583117983025801 1107 46-47 0.2902208130802393 1107 48-49 0.47762512992894557 1107 50-51 0.28531152497983214 1107 52-53 0.43271683666668537 1107 54-55 0.29174883672384766 1107 56-57 1.0740871518556432 1107 58-59 -6.795849278266115 1107 60-61 -9.083815888862915 1107 62-63 -2.3391226472366817 1107 64-65 0.22203203331426735 1107 66-67 -1.4244872558614183 1107 68-69 -3.9492719998191372 1107 70-71 -4.551083635560891 1107 72-73 -6.038405937040128 1107 74-75 -6.66256160857427 1107 76-77 -9.246732993118712 1107 78-79 -12.572377108833685 1107 80-81 -8.277707555581816 1107 82-83 -4.147804205318998 1107 84-85 -3.121528540888093 1107 86-87 -2.8429289445396684 1107 88-89 -2.904414016014343 1107 90-91 -8.505074380771056 1107 92-93 -11.976776686717615 1107 94-95 -11.97567399386682 1107 96-97 -12.457489547336447 1107 98-99 -11.723895332761069 1107 100-101 -11.239827788919703 1108 1 -0.01987908431597063 1108 2 -0.05566242261558685 1108 3 -0.10221506647996392 1108 4 -0.04597450055411656 1108 5 -0.15981524436192984 1108 6 0.0032680181010391607 1108 7 -0.032718375493907104 1108 8 -0.06831485389386671 1108 9 -0.01868878764545201 1108 10-11 -0.07000302404345149 1108 12-13 -0.07858619560128233 1108 14-15 0.14067667354466806 1108 16-17 0.01772593107421727 1108 18-19 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1.5586500365914908 2312 86-87 1.3436650373653798 2312 88-89 1.6718187870082595 2312 90-91 3.220481979569154 2312 92-93 5.0980977245188726 2312 94-95 9.041534208159543 2312 96-97 9.635601588172344 2312 98-99 9.92480345752052 2312 100-101 9.090746403754215 2313 1 -0.27759828989934476 2313 2 -0.280233322130929 2313 3 -0.3402631071318645 2313 4 -0.36025747600513824 2313 5 -0.4027084462827233 2313 6 -0.2860680239669193 2313 7 -0.4278070618762513 2313 8 -0.37689857196906473 2313 9 -0.4882339831294402 2313 10-11 -0.5495041005613999 2313 12-13 -0.49357084151775865 2313 14-15 -0.5236443845891969 2313 16-17 -0.5723522131673207 2313 18-19 -0.5012051697775775 2313 20-21 -0.4696961529371322 2313 22-23 -0.5790895543294994 2313 24-25 -0.5209614060660144 2313 26-27 -0.6108375481877246 2313 28-29 -0.6555670191046445 2313 30-31 -0.6461791933501999 2313 32-33 -0.44501866388856115 2313 34-35 -0.620817759081703 2313 36-37 -0.7478113922081278 2313 38-39 -0.9355130035669461 2313 40-41 -1.147317939113634 2313 42-43 -0.7566252072101278 2313 44-45 -0.7604320130966755 2313 46-47 -0.6625412498237537 2313 48-49 -0.6468571831892191 2313 50-51 -0.5415942163728076 2313 52-53 -0.7451576640731687 2313 54-55 -0.6368504256505787 2313 56-57 0.4018807140420435 2313 58-59 3.9279888640967293 2313 60-61 3.7103940841019387 2313 62-63 0.9159780113973177 2313 64-65 1.0014926237485007 2313 66-67 1.5714470420592086 2313 68-69 0.10932229776772573 2313 70-71 2.733607852262164 2313 72-73 8.117735797214404 2313 74-75 9.734158780709578 2313 76-77 8.434139849910558 2313 78-79 6.0617297300568715 2313 80-81 3.1702665468624716 2313 82-83 1.5802654101811555 2313 84-85 1.0619460872395834 2313 86-87 1.0005283833198781 2313 88-89 1.094570010442272 2313 90-91 2.6771304716482014 2313 92-93 4.601202950699918 2313 94-95 8.260447477935411 2313 96-97 8.994250631562787 2313 98-99 9.286509860657043 2313 100-101 8.50232005883794 2314 1 -0.3649202815423713 2314 2 -0.3930298678199655 2314 3 -0.4084419492074005 2314 4 -0.302868768889482 2314 5 -0.40036495063658606 2314 6 -0.39947578339549494 2314 7 -0.3581305842517466 2314 8 -0.4655894989166143 2314 9 -0.48177351865736284 2314 10-11 -0.44628340167680136 2314 12-13 -0.5544482156370449 2314 14-15 -0.6503720347259971 2314 16-17 -0.6430603163361681 2314 18-19 -0.599943259833573 2314 20-21 -0.6225996430431309 2314 22-23 -0.6447506472832742 2314 24-25 -0.6470433065486176 2314 26-27 -0.6690571408438117 2314 28-29 -0.80230112673798 2314 30-31 -0.8186430970237808 2314 32-33 -0.7417454866023974 2314 34-35 -0.8938962941113786 2314 36-37 -0.8765803037692024 2314 38-39 -0.8437771151755058 2314 40-41 -0.8688658765820421 2314 42-43 -0.7991398624895325 2314 44-45 -0.7751767800961744 2314 46-47 -0.6124266697319101 2314 48-49 -0.7235738609781279 2314 50-51 -0.6285842944923345 2314 52-53 -0.7028855696318317 2314 54-55 -0.6513067188317123 2314 56-57 0.622996278047701 2314 58-59 3.9191055343309067 2314 60-61 3.431453572214931 2314 62-63 1.2354753789331987 2314 64-65 0.8796875556030237 2314 66-67 1.5057439483967308 2314 68-69 -0.4870090257672395 2314 70-71 2.4956340401986985 2314 72-73 8.126838573937913 2314 74-75 9.656135718992818 2314 76-77 8.685132442676366 2314 78-79 6.20661490713438 2314 80-81 3.0853427327304495 2314 82-83 1.5928020821061537 2314 84-85 1.2642388132924332 2314 86-87 1.1822373894590008 2314 88-89 1.1691042252808437 2314 90-91 2.3226715163236413 2314 92-93 4.5212508692814986 2314 94-95 8.545731805645264 2314 96-97 9.11532316058105 2314 98-99 9.513712784338063 2314 100-101 8.6430508981506 2315 1 -0.6975624138414318 2315 2 -0.5037735226610884 2315 3 -0.5045312292381929 2315 4 -0.43745941454501747 2315 5 -0.6954923719373696 2315 6 -0.6926946608445519 2315 7 -0.7485708409447867 2315 8 -0.8767250769570722 2315 9 -1.0591618816100734 2315 10-11 -0.8770525185728033 2315 12-13 -0.6608163199722554 2315 14-15 -0.9548686040101657 2315 16-17 -1.073676771929307 2315 18-19 -0.882637759585613 2315 20-21 -0.9707950939213461 2315 22-23 -0.9839061434602598 2315 24-25 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2.2183722039471583 2315 92-93 4.1517905316132016 2315 94-95 8.154381687587193 2315 96-97 8.862464785859542 2315 98-99 9.0377102159273 2315 100-101 8.312893906818063 2316 1 -0.4561575450882458 2316 2 -0.4996018501415733 2316 3 -0.6295588361071296 2316 4 -0.418673735196343 2316 5 -0.6441384886867709 2316 6 -0.545631087380599 2316 7 -0.6533016079667107 2316 8 -0.6608859033014838 2316 9 -0.726505663348334 2316 10-11 -0.6821222478385494 2316 12-13 -0.7220819845818482 2316 14-15 -0.8036715162120416 2316 16-17 -0.9461612706483109 2316 18-19 -0.99290523112019 2316 20-21 -0.8374396076415351 2316 22-23 -0.9523049026639967 2316 24-25 -2.304294659782286 2316 26-27 -1.4166651521364741 2316 28-29 -1.2171591562355815 2316 30-31 -0.8811172133374541 2316 32-33 -0.7543067065385785 2316 34-35 -0.872878834176241 2316 36-37 -0.832462692406331 2316 38-39 -1.1249860256987105 2316 40-41 -0.9664535356885295 2316 42-43 -0.9974939961305793 2316 44-45 -1.0056853606898954 2316 46-47 -0.8261517919850689 2316 48-49 -1.1728086001060518 2316 50-51 -0.9907475731735147 2316 52-53 -0.8408866955371792 2316 54-55 -0.8476350630786769 2316 56-57 0.3546780034649686 2316 58-59 3.664354745917091 2316 60-61 3.608973322237798 2316 62-63 1.078937239123018 2316 64-65 0.7622610895454294 2316 66-67 5.9659022566632665 2316 68-69 8.96967288636733 2316 70-71 5.916207533063297 2316 72-73 9.227149878363672 2316 74-75 10.109795518197028 2316 76-77 8.72515207600092 2316 78-79 6.13024995695989 2316 80-81 3.015527078725082 2316 82-83 1.6434668423891416 2316 84-85 1.265740627251045 2316 86-87 1.138031974283738 2316 88-89 1.2586513704666196 2316 90-91 2.7549555450855756 2316 92-93 4.486988990793385 2316 94-95 8.403780021654782 2316 96-97 9.041090381984828 2316 98-99 9.187106642085618 2316 100-101 8.360853879652518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 25.0 4 43.0 5 146.0 6 302.0 7 766.0 8 1643.0 9 3056.0 10 5106.0 11 7689.0 12 10951.0 13 14891.0 14 19315.0 15 27185.0 16 36309.0 17 46401.0 18 48828.0 19 47355.0 20 48429.0 21 47915.0 22 46191.0 23 45043.0 24 47626.0 25 52096.0 26 68322.0 27 97206.0 28 134326.0 29 175294.0 30 209640.0 31 229102.0 32 239630.0 33 251010.0 34 257687.0 35 251824.0 36 213350.0 37 135802.0 38 44705.0 39 2074.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.8811957863246 20.644958289525185 15.333352403105211 22.140493521045006 2 14.916715088802594 22.156248757475204 40.34067326881854 22.586362884903664 3 18.975519228066233 26.732140783185205 30.8512623339445 23.44107765480406 4 12.224810029812101 18.142554019611538 38.69490794798716 30.937728002589203 5 12.311882933094155 39.329545573521045 34.73652617288518 13.62204532049962 6 29.45164249169076 38.376306547297276 18.10008753910487 14.071963421907096 7 26.6270753031083 34.259677772616115 21.60181104679956 17.511435877476032 8 25.211776846893947 35.64686757900412 20.290748711238184 18.850606862863746 9 25.964150145851907 17.248993126615638 21.041840175329195 35.74501655220326 10-11 24.00575176856769 27.712036304638666 28.02576437977018 20.256447547023463 12-13 25.69811515534518 25.21019833347981 28.26691526360064 20.82477124757437 14-15 22.317281109285535 26.367468621034444 27.50397849953632 23.811271770143694 16-17 20.664689888577794 29.852296173532377 27.95976133578303 21.523252602106798 18-19 20.539960362682326 28.588219189309612 30.55404181187707 20.317778636130992 20-21 22.002136510686736 27.175781190056682 30.12916019714769 20.692922102108888 22-23 21.271045990432786 27.501349705575855 29.956505999388973 21.271098304602393 24-25 21.237634674110627 27.555407680836137 30.34961210787042 20.85734553718282 26-27 21.215455585715695 27.473252590340625 30.57240171632627 20.73889010761741 28-29 20.669916033795744 28.13302060354524 30.450191387142624 20.746871975516395 30-31 21.017169482962274 27.70252677461677 30.295659155828087 20.98464458659287 32-33 20.889064820086613 27.708762565251245 30.19425187437931 21.20792074028283 34-35 21.38806614322895 27.56629363373601 30.1514597983268 20.894180424708235 36-37 21.333675898124998 27.276058732768416 30.456713519902074 20.933551849204513 38-39 20.991103531203574 27.65680972482447 30.478699300889577 20.87338744308238 40-41 21.123018064389644 27.647310609615154 30.363475069063156 20.866196256932042 42-43 20.90575177617198 27.625779767930737 29.939565150958046 21.528903304939234 44-45 21.021277055933492 27.658533105346393 29.792151753761548 21.528038084958563 46-47 21.28834185679846 27.84390084278781 29.377723379237768 21.490033921175957 48-49 21.160738409736016 27.80953434165535 29.4244938490566 21.605233399552034 50-51 20.86022311835459 28.02371417408534 29.546594619484836 21.56946808807524 52-53 21.181150621244218 28.323479539701268 28.969909099058967 21.52546073999554 54-55 20.805648760357244 27.830863674962576 29.275702332262316 22.087785232417865 56-57 21.23670670062104 27.962582192676805 28.994154973965735 21.806556132736425 58-59 20.060504684843025 27.63395790292365 30.530422880864354 21.77511453136897 60-61 21.25408325662741 27.767684432703277 29.377949067266464 21.600283243402853 62-63 20.968810161546834 27.833678608297756 29.294034764655002 21.903476465500404 64-65 20.283074139754103 28.01665614524969 29.611126682226143 22.089143032770068 66-67 20.80329668288532 27.583042851039853 29.41553576846092 22.198124697613903 68-69 21.63644981717868 27.04590288778091 29.447212001741086 21.870435293299327 70-71 20.73828258174769 27.318163727891427 29.814583276868717 22.128970413492166 72-73 20.628035263020152 27.157258806027272 29.917243803057413 22.297462127895166 74-75 20.913864641855863 27.599060108352557 29.166278411013398 22.320796838778183 76-77 19.329318987414105 27.742337827985125 30.037876556570215 22.890466628030556 78-79 20.15569063399287 27.89856170125281 29.076617212688298 22.86913045206602 80-81 20.6473058393752 28.06512573133221 28.460953212002504 22.82661521729009 82-83 20.808997769146703 27.927412954782888 28.375292545553055 22.88829673051735 84-85 20.732946057280888 27.802004086434884 28.199243083586023 23.265806772698205 86-87 20.67714631197098 27.934360557601483 28.169170785945497 23.219322344482038 88-89 20.772146815208707 27.757798605408624 28.03241316761829 23.43764141176438 90-91 20.534041398904904 27.646035028551935 28.33099478738458 23.48892878515859 92-93 20.48252247380022 27.90234399701223 28.417954864091634 23.19717866509592 94-95 19.40033924194742 27.789554803686823 29.052728431361984 23.757377523003772 96-97 18.937875079033724 27.74973773167036 29.40928694804438 23.90310024125154 98-99 19.412776543470383 27.750709907779804 28.676645945097647 24.15986760365217 100-101 19.18188812234454 27.642853365914092 28.993445678129113 24.181812833612252 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 869.0 1 633.5 2 430.0 3 476.5 4 531.0 5 630.0 6 897.5 7 1437.5 8 2072.0 9 2497.5 10 2710.0 11 2919.0 12 3216.5 13 3568.0 14 3846.5 15 4120.5 16 4386.5 17 4666.5 18 5007.0 19 5529.5 20 6192.0 21 7028.0 22 8229.0 23 9697.0 24 11489.5 25 13791.5 26 17206.5 27 21578.5 28 25937.5 29 30884.5 30 37392.5 31 44704.0 32 52909.5 33 61858.0 34 70112.0 35 77731.0 36 85268.0 37 91752.5 38 96802.5 39 100715.0 40 103700.0 41 107018.0 42 109921.0 43 112827.5 44 116075.0 45 118681.5 46 122039.5 47 125951.5 48 127748.5 49 122177.5 50 110500.0 51 98814.0 52 88713.5 53 79845.0 54 71123.5 55 62438.0 56 54175.5 57 46483.0 58 39044.0 59 31952.0 60 25561.0 61 19810.5 62 14801.5 63 10606.0 64 7494.0 65 5394.0 66 3744.5 67 2591.5 68 1857.0 69 1305.0 70 991.0 71 759.0 72 572.0 73 450.0 74 314.5 75 218.0 76 140.0 77 66.5 78 36.0 79 26.0 80 15.5 81 9.0 82 5.5 83 2.0 84 1.0 85 1.0 86 0.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021832446782539063 2 0.005091912508387705 3 0.0035922396463283126 4 0.0 5 0.06703188932274774 6 6.975222614229733E-5 7 0.0 8 1.7438056535574333E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.7438056535574333E-4 16-17 0.0 18-19 0.0015519870316661154 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7438056535574333E-4 28-29 0.002336699575766961 30-31 0.008980599115820782 32-33 3.3132307417591233E-4 34-35 0.0010288453355988857 36-37 0.02375063300145224 38-39 0.022634597383175484 40-41 0.024866668619729 42-43 0.025599066994223117 44-45 0.026279151199110517 46-47 0.02767419572195647 48-49 0.027220806252031535 50-51 0.02273922572238893 52-53 0.019007481623776024 54-55 0.013863254945781594 56-57 0.15451861896172417 58-59 0.7635253054101222 60-61 0.312943362587417 62-63 4.18513356853784E-4 64-65 0.14245148383910672 66-67 1.4234511169423971 68-69 2.72835832555596 70-71 2.2033333193828883 72-73 1.8547988834063638 74-75 1.5638100339972352 76-77 1.126254319406604 78-79 0.4212511317298691 80-81 2.61570848033615E-4 82-83 1.2206639574902033E-4 84-85 1.7438056535574333E-5 86-87 0.002894717384905339 88-89 1.7438056535574333E-5 90-91 0.22808977948531226 92-93 0.17295064471982624 94-95 1.2959440475542776 96-97 1.263282567663147 98-99 0.9954688953897963 100-101 0.4052255577736763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2867292.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.16267674088729 #Duplication Level Percentage of deduplicated Percentage of total 1 69.02258580655585 31.86267316408194 2 14.92926057387804 13.783492597048108 3 6.2395465466088496 8.641025107224719 4 3.1526563215670227 5.821402185904532 5 1.8590250935553876 4.290878722349756 6 1.1229930404200439 3.1104218824306002 7 0.780766068862198 2.5229576152997297 8 0.5670164319998561 2.0939997005744524 9 0.3877377633421145 1.6109111726457022 >10 1.7718376801935685 14.093928136415448 >50 0.10809280516693408 3.4228207481459605 >100 0.05387434726214178 4.584084399563619 >500 0.0021904606075053646 0.676092692592674 >1k 0.002039394358586169 1.7907913665401267 >5k 2.2659937317624098E-4 0.8010948742432565 >10k+ 1.5106624878416066E-4 0.8934256349394978 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14290 0.49837965578671445 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11333 0.39525098943532777 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9310 0.3246966126923941 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8539 0.2978071295145384 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5126 0.17877495560270806 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3880 0.13531931871605682 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3515 0.12258953744508755 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3280 0.11439365087336761 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.4876113071148665E-5 0.0 14-15 0.0 0.0 0.0 5.2314169606722995E-5 0.0 16-17 0.0 0.0 0.0 1.3950445228459466E-4 3.4876113071148665E-5 18-19 0.0 0.0 0.0 2.61570848033615E-4 3.4876113071148665E-5 20-21 0.0 0.0 0.0 4.7082752646050697E-4 3.4876113071148665E-5 22-23 0.0 0.0 0.0 0.0011683497878834802 3.4876113071148665E-5 24-25 0.0 0.0 0.0 0.002476204028051555 3.4876113071148665E-5 26-27 0.0 0.0 0.0 0.004603646925391624 3.4876113071148665E-5 28-29 0.0 0.0 0.0 0.01126498452198102 3.4876113071148665E-5 30-31 1.7438056535574333E-5 0.0 0.0 0.03541669282375147 3.4876113071148665E-5 32-33 3.4876113071148665E-5 0.0 0.0 0.0866148268121977 3.4876113071148665E-5 34-35 3.4876113071148665E-5 0.0 0.0 0.1618426027066654 3.4876113071148665E-5 36-37 3.4876113071148665E-5 0.0 0.0 0.2780672495162683 3.4876113071148665E-5 38-39 3.4876113071148665E-5 0.0 0.0 0.4608878342352296 3.4876113071148665E-5 40-41 3.4876113071148665E-5 0.0 0.0 0.6679821936517104 3.4876113071148665E-5 42-43 3.4876113071148665E-5 0.0 0.0 0.8711355522911514 3.4876113071148665E-5 44-45 3.4876113071148665E-5 0.0 0.0 1.1098102320935572 3.4876113071148665E-5 46-47 3.4876113071148665E-5 0.0 0.0 1.3877031010444698 3.4876113071148665E-5 48-49 3.4876113071148665E-5 0.0 0.0 1.6657354744476671 3.4876113071148665E-5 50-51 3.4876113071148665E-5 0.0 0.0 1.9404371790525694 3.4876113071148665E-5 52-53 3.4876113071148665E-5 5.2314169606723E-5 0.0 2.202147531538469 3.4876113071148665E-5 54-55 3.4876113071148665E-5 1.04628339213446E-4 0.0 2.480232219111273 3.4876113071148665E-5 56-57 3.4876113071148665E-5 1.04628339213446E-4 0.0 2.7291953522696675 3.4876113071148665E-5 58-59 3.4876113071148665E-5 1.04628339213446E-4 0.0 2.9035584795688756 3.4876113071148665E-5 60-61 3.4876113071148665E-5 1.04628339213446E-4 0.0 3.0373083731967307 3.4876113071148665E-5 62-63 3.4876113071148665E-5 1.04628339213446E-4 0.0 3.150969625695604 5.2314169606722995E-5 64-65 3.4876113071148665E-5 1.3950445228459466E-4 3.4876113071148665E-5 3.267176834448671 6.975222614229733E-5 66-67 3.4876113071148665E-5 1.3950445228459466E-4 3.4876113071148665E-5 3.3634174684685063 6.975222614229733E-5 68-69 3.4876113071148665E-5 1.3950445228459466E-4 6.975222614229733E-5 3.4750559064092528 6.975222614229733E-5 70-71 3.4876113071148665E-5 1.3950445228459466E-4 6.975222614229733E-5 3.6814701816208464 6.975222614229733E-5 72-73 3.4876113071148665E-5 1.3950445228459466E-4 6.975222614229733E-5 3.9352462183830594 6.975222614229733E-5 74-75 3.4876113071148665E-5 1.3950445228459466E-4 6.975222614229733E-5 4.227054656449361 8.719028267787167E-5 76-77 3.4876113071148665E-5 1.3950445228459466E-4 6.975222614229733E-5 4.639394941289551 1.04628339213446E-4 78-79 3.4876113071148665E-5 1.3950445228459466E-4 6.975222614229733E-5 5.215670395620676 1.04628339213446E-4 80-81 3.4876113071148665E-5 1.3950445228459466E-4 6.975222614229733E-5 5.859326500405261 1.04628339213446E-4 82-83 3.4876113071148665E-5 1.7438056535574333E-4 6.975222614229733E-5 6.465944173108285 1.04628339213446E-4 84-85 3.4876113071148665E-5 1.7438056535574333E-4 6.975222614229733E-5 6.966433833735804 1.04628339213446E-4 86-87 3.4876113071148665E-5 1.7438056535574333E-4 6.975222614229733E-5 7.413998992777855 1.04628339213446E-4 88-89 3.4876113071148665E-5 1.7438056535574333E-4 6.975222614229733E-5 7.858965881396104 1.04628339213446E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15675 0.0 24.033321 1 TACATGG 15855 0.0 23.606016 2 ACATGGG 15925 0.0 22.732656 3 GAGTACT 11925 0.0 20.935297 12-13 AGTACTT 12205 0.0 20.628685 12-13 GTATCAA 27365 0.0 20.382801 1 CATGGGA 11785 0.0 20.038675 4 GTACTTT 12570 0.0 19.823576 14-15 GGTATCA 21095 0.0 19.311178 1 CATGGGG 4295 0.0 18.657207 4 CTCGCGA 95 1.5713314E-4 17.899256 70-71 ATCAACG 31150 0.0 17.67475 3 CAACGCA 31245 0.0 17.635788 5 TCAACGC 31220 0.0 17.634811 4 AACGCAG 31370 0.0 17.582985 6 ACTTTTT 14655 0.0 17.372717 16-17 TATCAAC 31970 0.0 17.29513 2 AGAGTAC 24255 0.0 17.264671 10-11 CGCGCGT 275 6.4568485E-7 17.128431 7 TACTTTT 14770 0.0 17.062195 14-15 >>END_MODULE