FastQCFastQC Report
Fri 27 May 2016
SRR939810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087205
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138600.44894977819743104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99200.3213262481759391No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT85170.275880610455088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70880.22959278700313066No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49180.159302670214644No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37970.12299150850040733No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33150.10737868071605222No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32060.10384797899718352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG163150.026.5842931
TACATGG166850.025.9669972
ACATGGG170200.024.0190353
GAGTACT117000.022.71810212-13
GTACTTT126100.021.0646114-15
AGTACTT123300.020.8896212-13
CATGGGA123850.020.814834
GTATCAA311800.020.7899951
CATGGGG48000.019.6009464
TATCAAC350000.018.386812
ACTTTTT155350.018.1765516-17
ATCAACG354450.018.1695353
CAACGCA353750.018.0988085
TCAACGC356000.018.0637054
AACGCAG358500.017.8995346
AGAGTAC250000.017.51953910-11
ATGGGAG46600.017.2622935
GTATCGA1650.001562845417.1060051
AGTACAT121800.016.9166812
TACTTTT152200.016.88039214-15
Produced by FastQC (version 0.11.5)