FastQCFastQC Report
Fri 27 May 2016
SRR939810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087205
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152310.4933588796338435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121410.3932683446677496No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98610.3194151344015056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90140.2919793146227737No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56500.18301343772117498No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42930.1390578209092043No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37300.1208212606548642No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35640.1154442286793394No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCG856.458395E-427.9049938
CATTCCG4000.027.2771329
GTACATG166400.025.6380461
TACATGG171900.024.5653172
ACATGGG170150.023.6704543
GAGTACT125650.022.23774112-13
CGATCGT1259.649739E-920.87327424-25
GTACTTT135350.020.59147614-15
CATGGGG47950.020.5781124
AGTACTT131450.020.552812-13
CATGGGA125550.019.3456844
GTGTAGC17950.019.0347251
CGCATAG2400.018.93536866-67
GCACCGT4550.018.7668746
GTATCAA302850.018.7405431
GGTATCA244100.018.3325331
ACTTTTT156750.018.02237716-17
ATGGGAT49400.017.3813085
TACACCG2755.995116E-717.2503595
TACTTTT158800.017.04288114-15
Produced by FastQC (version 0.11.5)