##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939810_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3087205 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37611755617136 31.0 31.0 34.0 30.0 34.0 2 31.593580925141026 31.0 31.0 34.0 30.0 34.0 3 31.634652379741546 31.0 31.0 34.0 30.0 34.0 4 35.44543138534694 37.0 35.0 37.0 33.0 37.0 5 35.185824718475125 37.0 35.0 37.0 32.0 37.0 6 35.21570967914343 37.0 35.0 37.0 32.0 37.0 7 35.12644997659695 37.0 35.0 37.0 32.0 37.0 8 35.09416899752365 37.0 35.0 37.0 32.0 37.0 9 36.65245651001472 39.0 35.0 39.0 32.0 39.0 10-11 36.523235580403636 38.0 35.0 39.0 32.0 39.0 12-13 36.44492574999069 38.0 35.0 39.0 32.0 39.0 14-15 37.414654355638845 39.0 36.0 41.0 32.0 41.0 16-17 37.35445815875525 39.0 36.0 41.0 32.0 41.0 18-19 37.38078051182218 39.0 36.0 41.0 32.0 41.0 20-21 37.32321144854326 39.0 36.0 40.5 31.5 41.0 22-23 37.19429710693005 39.0 36.0 40.0 31.0 41.0 24-25 37.04177322205685 39.0 36.0 40.0 31.0 41.0 26-27 36.74179038968906 39.0 35.5 40.0 30.0 41.0 28-29 36.523720485034204 38.0 35.0 40.0 30.0 41.0 30-31 36.25946009416285 38.0 35.0 40.0 30.0 41.0 32-33 36.22363254140882 38.0 35.0 40.0 30.0 41.0 34-35 36.06803532645225 38.0 35.0 40.0 30.0 41.0 36-37 36.08493005809462 38.0 35.0 40.0 30.0 41.0 38-39 35.976803937542215 38.0 35.0 40.0 29.5 41.0 40-41 35.90553348417096 38.0 35.0 40.0 29.0 41.0 42-43 35.67512555855539 38.0 34.5 40.0 28.5 41.0 44-45 35.39593629188862 38.0 34.0 40.0 27.0 41.0 46-47 35.075312944880565 38.0 34.0 40.0 26.5 41.0 48-49 34.75051397623417 38.0 33.5 40.0 25.0 41.0 50-51 34.53099437841024 38.0 33.0 40.0 24.0 41.0 52-53 34.29728459885236 38.0 33.0 40.0 24.0 41.0 54-55 34.0600996694421 38.0 33.0 40.0 23.5 41.0 56-57 33.722574626563514 37.0 32.5 40.0 23.0 41.0 58-59 33.387507632308186 37.0 32.0 40.0 22.0 41.0 60-61 33.079274295033855 36.5 31.5 39.0 21.5 41.0 62-63 32.80896782040713 36.0 31.0 39.0 20.5 40.5 64-65 32.39458134461431 36.0 31.0 39.0 18.5 40.0 66-67 31.500587586506242 35.0 30.0 38.5 12.5 40.0 68-69 29.644933038136436 34.0 26.5 38.0 7.5 40.0 70-71 28.986055185839618 34.0 25.5 37.0 7.5 39.0 72-73 29.523888760221624 34.0 27.0 36.5 7.5 39.0 74-75 29.60947701885686 34.0 28.0 36.0 7.5 39.0 76-77 29.107681867579252 32.5 27.5 35.5 7.0 38.0 78-79 29.621948007987807 34.0 29.0 36.0 7.0 38.0 80-81 29.40940397544057 34.0 29.0 35.0 3.5 37.0 82-83 28.94816784113786 34.0 28.5 35.0 2.0 37.0 84-85 28.56059947428175 33.0 28.0 35.0 2.0 36.0 86-87 28.16098088724267 33.0 27.0 35.0 2.0 36.0 88-89 27.67954800539647 33.0 26.0 35.0 2.0 35.5 90-91 27.166439222533004 33.0 26.0 35.0 2.0 35.0 92-93 26.67455352009342 32.0 25.0 34.5 2.0 35.0 94-95 25.865454836980376 32.0 23.5 34.0 2.0 35.0 96-97 25.313246771756333 32.0 21.0 34.0 2.0 35.0 98-99 24.635696528089323 31.5 17.5 34.0 2.0 35.0 100-101 23.181228489847612 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.349704645182662 1101 2 0.3501092011748774 1101 3 0.3862522633746188 1101 4 0.3012752294342249 1101 5 0.32999281197347585 1101 6 0.23894024538814307 1101 7 0.25970622046674663 1101 8 0.1975953765833225 1101 9 0.44583432790796707 1101 10-11 0.43998162522213136 1101 12-13 0.46646979310182246 1101 14-15 0.4760966297799385 1101 16-17 0.4531914446945535 1101 18-19 0.3972349527121395 1101 20-21 0.44406604934857796 1101 22-23 0.39100216597527293 1101 24-25 0.39141411086781375 1101 26-27 0.48828438928173057 1101 28-29 0.4360247037892506 1101 30-31 0.3382460453851124 1101 32-33 0.2968589767752903 1101 34-35 0.2529362298987863 1101 36-37 0.5093987963128583 1101 38-39 0.48582483677739674 1101 40-41 0.4823436559368659 1101 42-43 0.308493447789985 1101 44-45 0.2356660609151291 1101 46-47 0.05487859641035442 1101 48-49 0.3519262226967115 1101 50-51 0.4045855570834007 1101 52-53 0.0783498857144238 1101 54-55 -0.05208010084670889 1101 56-57 -0.05259120975439657 1101 58-59 0.20464860391942352 1101 60-61 0.16170952438821473 1101 62-63 0.13934956468913384 1101 64-65 -0.05759410192571579 1101 66-67 -0.018475601303688194 1101 68-69 -8.65362727915186 1101 70-71 -6.469370029261089 1101 72-73 -1.4806827902691886 1101 74-75 -0.5071071718447548 1101 76-77 -0.35396485305198766 1101 78-79 -0.4748847157790905 1101 80-81 -0.39571947410199115 1101 82-83 -0.36151622017436225 1101 84-85 -0.23350723384566052 1101 86-87 -0.32721901127825603 1101 88-89 -0.03804197183255553 1101 90-91 -0.34124815558069344 1101 92-93 -0.6299110972985744 1101 94-95 -0.5200974649413901 1101 96-97 -0.723480102180261 1101 98-99 -0.5142463465636702 1101 100-101 -0.5193352004043277 1102 1 0.2178866433189377 1102 2 0.14482151009915967 1102 3 0.22632105013466486 1102 4 0.12555160609263538 1102 5 0.34618877372889045 1102 6 0.2799973843765926 1102 7 0.2742909698072751 1102 8 0.18870692684379975 1102 9 0.3348509693401738 1102 10-11 0.3286178368801487 1102 12-13 0.349915392309498 1102 14-15 0.4975603878139623 1102 16-17 0.4742328541511043 1102 18-19 0.306004000400101 1102 20-21 0.29977753619855463 1102 22-23 0.4371288186490361 1102 24-25 0.3161628725165002 1102 26-27 0.45788177913757977 1102 28-29 0.1878703787169158 1102 30-31 0.09942663857609801 1102 32-33 0.07266191315418524 1102 34-35 0.01350218031948458 1102 36-37 0.026341977371146186 1102 38-39 0.33310199425669396 1102 40-41 0.3087109824254597 1102 42-43 0.19412996984676312 1102 44-45 0.1861621167216967 1102 46-47 0.26152083040310714 1102 48-49 0.15700212400920321 1102 50-51 0.14230259917287214 1102 52-53 0.27158974677022485 1102 54-55 0.03106684352205491 1102 56-57 0.03699226705103342 1102 58-59 0.001510582267357563 1102 60-61 0.08389757945887055 1102 62-63 0.1463075869999173 1102 64-65 0.1173581821666616 1102 66-67 0.45678149237252796 1102 68-69 -4.317679177291829 1102 70-71 0.38818006287710816 1102 72-73 0.6528492248841253 1102 74-75 0.4924944331796439 1102 76-77 0.22352421728543348 1102 78-79 0.05628856823643957 1102 80-81 0.15372258995326504 1102 82-83 0.09841006926965079 1102 84-85 -0.042695336490840674 1102 86-87 -0.07187477972144762 1102 88-89 -0.17271760513085965 1102 90-91 -0.2998470162542368 1102 92-93 -0.2394010153273527 1102 94-95 0.04651001636731067 1102 96-97 -0.36203039692600925 1102 98-99 -0.13059780711790836 1102 100-101 -0.05378863077636353 1103 1 0.09187813921075261 1103 2 0.08641224722843788 1103 3 0.10405787036549086 1103 4 0.08755369482096143 1103 5 0.04000656415270498 1103 6 0.046116825535193584 1103 7 0.009537620915047285 1103 8 0.11273955789551593 1103 9 0.23665057930212896 1103 10-11 0.20176670140054398 1103 12-13 0.25124445227517356 1103 14-15 0.13271938692945184 1103 16-17 0.20534382317185162 1103 18-19 0.16605291500734864 1103 20-21 0.10949534473888889 1103 22-23 0.02389645827945941 1103 24-25 0.02354418266068592 1103 26-27 0.2042321526576174 1103 28-29 0.03445386923771565 1103 30-31 0.08764344005099645 1103 32-33 0.15036713604068552 1103 34-35 -0.004962231373127679 1103 36-37 0.053792130295896357 1103 38-39 0.2773749889176429 1103 40-41 0.27706866649464956 1103 42-43 0.1233552221459604 1103 44-45 0.295826268388943 1103 46-47 0.23581092919143742 1103 48-49 0.10299299115116156 1103 50-51 0.1151460937432418 1103 52-53 0.0832617418254884 1103 54-55 0.13766440098871868 1103 56-57 0.24071840919112475 1103 58-59 0.2947813070347749 1103 60-61 0.09684428491620878 1103 62-63 0.06175543806079986 1103 64-65 -0.05537805194141754 1103 66-67 0.11879583639460733 1103 68-69 -4.137617847539175 1103 70-71 0.259922978784477 1103 72-73 0.41335195834747296 1103 74-75 0.30939538909255404 1103 76-77 0.1555256666071294 1103 78-79 0.06262029239642075 1103 80-81 0.08457284092036943 1103 82-83 -0.09049254910050308 1103 84-85 -0.024020666584512185 1103 86-87 -0.0437535162539433 1103 88-89 -0.13256979563140803 1103 90-91 -0.4581845057306708 1103 92-93 -0.51107066589163 1103 94-95 -0.5855905942660193 1103 96-97 -0.6376897850138619 1103 98-99 -0.38210387615251307 1103 100-101 -0.5127670188322462 1104 1 0.2241113898310978 1104 2 0.2237054647635901 1104 3 0.23039782073769643 1104 4 0.18605038630152393 1104 5 0.29109423991780403 1104 6 0.22458043182138 1104 7 0.20894415524093546 1104 8 0.1444393097565566 1104 9 0.28989499617558323 1104 10-11 0.34082067162386664 1104 12-13 0.30311686749436717 1104 14-15 0.45257153746708667 1104 16-17 0.2894102003430632 1104 18-19 0.27186763791884516 1104 20-21 0.3408652785685007 1104 22-23 0.3311142002149481 1104 24-25 0.23017776413298208 1104 26-27 0.22230183834827955 1104 28-29 0.25185668147923224 1104 30-31 0.30494079323544554 1104 32-33 0.20987395986202984 1104 34-35 0.1892034013812136 1104 36-37 0.2767563569294751 1104 38-39 0.3165995546744682 1104 40-41 0.2294280544185483 1104 42-43 0.15157482512363174 1104 44-45 0.28722101611433715 1104 46-47 0.1872160160400682 1104 48-49 0.3117688389592601 1104 50-51 0.30074472897897664 1104 52-53 0.22191041841770698 1104 54-55 0.17727151430550947 1104 56-57 0.1695232065443122 1104 58-59 0.14634044681972114 1104 60-61 0.13193733702539134 1104 62-63 0.1895180517911612 1104 64-65 0.411872775188435 1104 66-67 0.5033864071142133 1104 68-69 -3.9863295927831786 1104 70-71 0.8541134254346012 1104 72-73 0.9717504281572324 1104 74-75 0.6320318655193553 1104 76-77 0.47371528612739766 1104 78-79 0.16742281514249058 1104 80-81 0.26116299807504006 1104 82-83 0.17159181814515634 1104 84-85 0.07024112001598226 1104 86-87 0.027349668196016808 1104 88-89 0.04958487765230046 1104 90-91 0.18996375729662418 1104 92-93 0.06954761367826023 1104 94-95 0.5486060558580483 1104 96-97 -0.05840421842990651 1104 98-99 0.24737813377304718 1104 100-101 0.2382338082976112 1105 1 0.20433324606051073 1105 2 0.23759246986595883 1105 3 0.15181808812774022 1105 4 0.10373026100280924 1105 5 0.1831096762027613 1105 6 0.1822202626035292 1105 7 0.1704834241164761 1105 8 0.22685550816772349 1105 9 0.20014736974659542 1105 10-11 0.17069739125184924 1105 12-13 0.1880200677785524 1105 14-15 0.3072997070724526 1105 16-17 0.20515206238530226 1105 18-19 0.11134238441499633 1105 20-21 0.1430979692615253 1105 22-23 0.25531130348950626 1105 24-25 0.18854662029779945 1105 26-27 0.23330463173456195 1105 28-29 0.32599283080430297 1105 30-31 0.18612499526347648 1105 32-33 0.17323014083470412 1105 34-35 0.22974423649068854 1105 36-37 0.15774800906407904 1105 38-39 0.26369314118871046 1105 40-41 0.24374779219546383 1105 42-43 0.16361706499962736 1105 44-45 0.1783439412580421 1105 46-47 0.2894188352611451 1105 48-49 0.3984736119360761 1105 50-51 0.47593243484993053 1105 52-53 0.30234823731585436 1105 54-55 0.40595216405039025 1105 56-57 0.30489022198059956 1105 58-59 0.3102678187760617 1105 60-61 0.3524057539988448 1105 62-63 0.39328993062600404 1105 64-65 0.30407591734435613 1105 66-67 0.7367447678970045 1105 68-69 2.0931043024567657 1105 70-71 2.355081018924235 1105 72-73 1.3643507801561974 1105 74-75 0.8045396159553171 1105 76-77 0.5892335157986501 1105 78-79 0.6042256795744478 1105 80-81 0.5629270295934852 1105 82-83 0.6198891442447483 1105 84-85 0.47927522193421623 1105 86-87 0.4626379682670603 1105 88-89 0.5405806472060029 1105 90-91 0.4700729900034659 1105 92-93 0.6083280502861541 1105 94-95 0.6687656663986452 1105 96-97 0.2573384509494119 1105 98-99 0.5142874052320678 1105 100-101 0.4784690663225426 1106 1 0.4465098782533268 1106 2 0.41635332547524584 1106 3 0.48738950198753983 1106 4 0.3130111892638112 1106 5 0.32798269765196153 1106 6 0.25594154927300394 1106 7 0.40305984420083973 1106 8 0.36718208791477025 1106 9 0.4318003830735506 1106 10-11 0.358239798408718 1106 12-13 0.5072383580216382 1106 14-15 0.5966347587001906 1106 16-17 0.48017508786239915 1106 18-19 0.5367665701768232 1106 20-21 0.5319801622835385 1106 22-23 0.5400554300204234 1106 24-25 0.5569651541251162 1106 26-27 0.5466524115304523 1106 28-29 0.5857333965874076 1106 30-31 0.7093243053711902 1106 32-33 0.7926634358822113 1106 34-35 0.7809035142423042 1106 36-37 0.7043206180063564 1106 38-39 0.8408929047467524 1106 40-41 0.7225647685122354 1106 42-43 0.7415381135661505 1106 44-45 0.8037036494552794 1106 46-47 0.7600407453002518 1106 48-49 0.7224621104092108 1106 50-51 0.703943675186089 1106 52-53 0.6888812710895991 1106 54-55 0.8215257434262071 1106 56-57 0.806357135885662 1106 58-59 0.9328224778121381 1106 60-61 1.015491637159812 1106 62-63 0.8786776804908456 1106 64-65 0.8422049862513674 1106 66-67 1.1990659830608514 1106 68-69 2.702115143324761 1106 70-71 2.959443734344287 1106 72-73 1.9011921144794819 1106 74-75 1.3157846236415516 1106 76-77 0.9443179833806887 1106 78-79 0.8457653739190256 1106 80-81 0.8730511387836586 1106 82-83 0.9963090313638503 1106 84-85 0.8644893256514301 1106 86-87 0.98088529552615 1106 88-89 1.1149637865772029 1106 90-91 0.971968595106361 1106 92-93 0.9447330736189166 1106 94-95 1.2407452490914572 1106 96-97 1.0560069700354333 1106 98-99 1.3786294018752017 1106 100-101 1.5237692010288484 1107 1 0.10385873709995153 1107 2 0.196763293894211 1107 3 0.08848038010251358 1107 4 0.11754855655009777 1107 5 0.16935266780190972 1107 6 0.08780926919405374 1107 7 0.026880518283228128 1107 8 0.10732741718911143 1107 9 0.1134071999641435 1107 10-11 0.1571440046304602 1107 12-13 0.12171013376404005 1107 14-15 0.16535095235504826 1107 16-17 0.24202265041290616 1107 18-19 0.17284704504670145 1107 20-21 0.16753283356315052 1107 22-23 0.28361698772689437 1107 24-25 0.29511433547286003 1107 26-27 0.18438856162287465 1107 28-29 0.15651621701643847 1107 30-31 0.2146861596350007 1107 32-33 0.2385128882321581 1107 34-35 0.24261779719565624 1107 36-37 0.1192395195404714 1107 38-39 0.23962961079229217 1107 40-41 0.1510427079502108 1107 42-43 0.21841354152032721 1107 44-45 0.22701315757699803 1107 46-47 0.18804844062407966 1107 48-49 0.36123359582287407 1107 50-51 0.27213306308832586 1107 52-53 0.3507158827579744 1107 54-55 0.3169714023966037 1107 56-57 0.44403105242042784 1107 58-59 0.26894849609231386 1107 60-61 0.42167898674235715 1107 62-63 0.3835482835324413 1107 64-65 0.3076289669365835 1107 66-67 0.8221883283532172 1107 68-69 2.249276941226512 1107 70-71 2.5642968804790804 1107 72-73 1.5400790339519865 1107 74-75 1.0273454759293337 1107 76-77 0.6204000358843835 1107 78-79 0.7988877496716995 1107 80-81 0.8156653914552656 1107 82-83 0.8780472057012112 1107 84-85 0.7718422546252448 1107 86-87 0.7035519231195977 1107 88-89 0.8292585039092337 1107 90-91 0.7194625258902505 1107 92-93 0.7073768055652749 1107 94-95 0.9404216450951886 1107 96-97 0.7074514660523263 1107 98-99 0.9771565725213023 1107 100-101 0.9333667920177255 1108 1 -0.0889523756716919 1108 2 -0.2543824026535475 1108 3 -0.1747622630809218 1108 4 -0.0012861592667263722 1108 5 -0.06773128415161978 1108 6 0.01735483221773393 1108 7 -0.05448743349553098 1108 8 -0.06200729242174674 1108 9 -0.1966565620223193 1108 10-11 -0.1307505924778809 1108 12-13 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2316 72-73 0.6501266475637202 2316 74-75 0.2617213601058985 2316 76-77 -0.1398223913661809 2316 78-79 -0.4370951942502863 2316 80-81 -0.5979557591153188 2316 82-83 -0.39790962386704365 2316 84-85 -0.36394225192860574 2316 86-87 -0.469726809669492 2316 88-89 -0.27922502889270007 2316 90-91 -0.4025871268223291 2316 92-93 -0.39087289441434336 2316 94-95 -0.16406621732052074 2316 96-97 -0.3411604513897366 2316 98-99 -0.5676060868311801 2316 100-101 -0.6587430879878298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 21.0 4 57.0 5 134.0 6 340.0 7 778.0 8 1805.0 9 3302.0 10 5419.0 11 8055.0 12 11431.0 13 14508.0 14 18332.0 15 23308.0 16 29194.0 17 34514.0 18 30481.0 19 24248.0 20 23417.0 21 24549.0 22 26669.0 23 30803.0 24 36295.0 25 44630.0 26 54803.0 27 67731.0 28 83266.0 29 100150.0 30 120129.0 31 141702.0 32 169866.0 33 205078.0 34 252780.0 35 318890.0 36 403197.0 37 461484.0 38 291344.0 39 24489.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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21.528022628974384 18-19 20.55694390233237 28.58086845544757 30.548748787333523 20.313438854886538 20-21 22.001357214697435 27.215410055373713 30.13345404662146 20.649778683307392 22-23 21.2652382980722 27.437018273810775 30.011482878526046 21.286260549590974 24-25 21.23231995060899 27.563976630615137 30.352618706618273 20.851084712157597 26-27 21.250620911640002 27.456611496295423 30.565955594958467 20.72681199710611 28-29 20.643093649200797 28.127119637782044 30.48329846896964 20.746488244047523 30-31 20.997421280380774 27.693233104139004 30.300587168076092 21.00875844740413 32-33 20.89013024222316 27.726603895362445 30.212341064078075 21.170924798336323 34-35 21.417245397177194 27.556798948388977 30.145999138990238 20.87995651544359 36-37 21.293543114023787 27.22767421663371 30.501729573691737 20.977053095650767 38-39 21.021985726501615 27.683875647920374 30.435653756215782 20.858484869362233 40-41 21.11523692271377 27.657989816825424 30.368239493636246 20.858533766824564 42-43 20.906906367468668 27.649642501281583 29.92755993952142 21.515891191728326 44-45 21.02505193282177 27.654734195059905 29.793001462581298 21.52721240953703 46-47 21.28000370892463 27.858027941430112 29.346558156846015 21.51541019279924 48-49 21.205051496507053 27.82714486376343 29.396120680222378 21.57168295950714 50-51 20.910662872791885 28.013981767549467 29.532731247804506 21.542624111854142 52-53 21.152743441028 28.320085029443614 28.96819467048984 21.558976859038545 54-55 20.77517764332358 27.86980990446607 29.28452295912745 22.0704894930829 56-57 21.177645648370163 28.056136996954184 28.88934612144385 21.876871233231807 58-59 21.381698515533735 27.846944018432236 28.85024326430964 21.921114201724393 60-61 21.075834057361142 27.909182616335233 29.30867816170149 21.706305164602135 62-63 21.04038041682593 27.824685631361067 29.27902524666844 21.855908705144568 64-65 20.75353064633738 28.04018475945088 29.060838518179704 22.145446076032034 66-67 20.637000448655833 27.990511723931654 28.91823661064038 22.454251216772136 68-69 20.517617214337694 27.972014098649893 29.163097962850788 22.34727072416162 70-71 20.732400100284238 27.809153635514928 28.949285247193814 22.509161017007024 72-73 20.70692837180626 27.566604607016394 29.10201714144327 22.624449879734076 74-75 20.740520408515387 28.209264419245567 28.366137723517724 22.68407744872132 76-77 20.719742534813772 27.82119357114679 28.524313951350226 22.93474994268921 78-79 20.81163566676077 27.885095215442828 28.464455509032888 22.838813608763516 80-81 20.727876931409213 28.073050394432546 28.324284823266975 22.874787850891266 82-83 20.786764068764523 27.919467543836205 28.397570292198193 22.896198095201076 84-85 20.740647883884012 27.806881923976796 28.17579357791182 23.276676614227377 86-87 20.69011536474403 27.98117366580665 28.155168253073615 23.173542716375703 88-89 20.77022993364527 27.75344608941126 28.030072606362733 23.446251370580736 90-91 20.65114053815404 27.65299303604613 28.22971621701726 23.46615020878257 92-93 20.82409605303052 27.839305658851853 28.147893446595024 23.188704841522608 94-95 20.45971955282235 27.629561026938347 28.282492003686393 23.628227416552914 96-97 20.346919350208996 27.49404371205843 28.36521359374909 23.793823343983487 98-99 20.597091374914832 27.564226548647337 27.858464925794067 23.980217150643764 100-101 20.328217976621392 27.363473460189514 28.24457304240542 24.063735520783677 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1500.0 1 1030.0 2 681.5 3 829.5 4 732.5 5 768.0 6 1367.5 7 2899.5 8 4356.5 9 4801.0 10 4318.5 11 3632.0 12 3570.0 13 3794.0 14 4037.0 15 4246.0 16 4485.5 17 4686.0 18 4941.0 19 5502.0 20 6295.0 21 7274.5 22 8426.0 23 9763.5 24 11467.0 25 13997.0 26 18168.0 27 23238.5 28 27302.0 29 32515.5 30 39584.5 31 46783.0 32 55253.0 33 64830.0 34 73885.5 35 81114.5 36 89188.5 37 96593.5 38 100288.0 39 103260.0 40 106081.0 41 109151.5 42 111874.0 43 115033.5 44 118595.5 45 120101.5 46 121740.5 47 130207.5 48 146653.5 49 142729.5 50 121264.5 51 106964.0 52 96051.0 53 87418.5 54 79801.5 55 71156.0 56 61865.5 57 53510.0 58 46699.5 59 40047.0 60 33060.5 61 26289.5 62 19972.5 63 14581.0 64 10058.5 65 6983.0 66 5057.0 67 3728.5 68 2637.0 69 1772.0 70 1302.5 71 1046.5 72 875.0 73 705.0 74 522.0 75 398.5 76 266.5 77 144.5 78 74.5 79 45.0 80 32.0 81 18.5 82 12.0 83 9.5 84 7.0 85 3.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02309532408764562 2 0.02426142740763895 3 9.717527666611061E-5 4 0.0 5 0.0 6 9.717527666611061E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.048969861087942E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.1337115611046238E-4 26-27 3.401134683313871E-4 28-29 9.717527666611061E-5 30-31 4.534846244418495E-4 32-33 0.0023484025194310063 34-35 0.0042757121733088665 36-37 0.09923215335554329 38-39 0.09126378066892221 40-41 0.10034966903720356 42-43 0.1016453393927517 44-45 0.09497263706167877 46-47 0.08917451222060083 48-49 0.08980615151893055 50-51 0.08938505865337741 52-53 0.06983663216404483 54-55 0.0585966918296647 56-57 0.03255371768314705 58-59 0.014219982152140851 60-61 0.009944269978831986 62-63 0.00210546432776573 64-65 0.00531224845774738 66-67 0.1510751634569133 68-69 0.4030344599726937 70-71 0.38695195168445246 72-73 0.17791173569620417 74-75 0.03098271737704493 76-77 0.031177067930377157 78-79 0.005814320720522285 80-81 0.0015710003061021214 82-83 8.097939722175883E-5 84-85 5.182681422192566E-4 86-87 0.0012956703555481413 88-89 3.2391758888703534E-4 90-91 7.126186955514778E-4 92-93 0.002494165434430172 94-95 0.0759910663528985 96-97 0.01784785914767565 98-99 0.019175921262112493 100-101 0.010478734000495593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3087205.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.549195929681616 #Duplication Level Percentage of deduplicated Percentage of total 1 69.12090546785811 31.484016659924706 2 14.648930567144102 13.344940171262976 3 6.145638626170401 8.39786693689365 4 3.177305967844596 5.7889492823160085 5 1.8771256244496868 4.275078142634237 6 1.2181520270244837 3.329150721064622 7 0.8136897523457519 2.594403976889954 8 0.57334849466347 2.089245033553153 9 0.40400046580296756 1.656170673528781 >10 1.8493049866909768 14.525339687539965 >50 0.1108574553599712 3.500400648836832 >100 0.0556106848431132 4.650639814457289 >500 0.0027074365627265265 0.810741887713267 >1k 0.0020662015866824484 1.868840858780552 >5k 2.137449917257705E-4 0.7959108474558582 >10k+ 1.4249666115051366E-4 0.8883046571482873 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15231 0.4933588796338435 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12141 0.3932683446677496 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9861 0.3194151344015056 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9014 0.2919793146227737 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5650 0.18301343772117498 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4293 0.1390578209092043 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3730 0.1208212606548642 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3564 0.1154442286793394 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.2391758888703535E-5 0.0 8 0.0 0.0 0.0 3.2391758888703535E-5 0.0 9 0.0 0.0 0.0 3.2391758888703535E-5 0.0 10-11 0.0 0.0 0.0 4.8587638333055306E-5 0.0 12-13 0.0 0.0 0.0 1.9435055333222122E-4 0.0 14-15 0.0 0.0 0.0 2.2674231222092475E-4 0.0 16-17 0.0 0.0 0.0 3.8870110666444245E-4 1.6195879444351767E-5 18-19 0.0 0.0 0.0 4.8587638333055306E-4 3.2391758888703535E-5 20-21 1.6195879444351767E-5 0.0 0.0 7.450104544401814E-4 3.2391758888703535E-5 22-23 3.2391758888703535E-5 0.0 0.0 0.0016033920649908251 3.2391758888703535E-5 24-25 3.2391758888703535E-5 0.0 0.0 0.0028018871438728558 3.2391758888703535E-5 26-27 3.2391758888703535E-5 0.0 0.0 0.004567238003307199 3.2391758888703535E-5 28-29 3.2391758888703535E-5 0.0 0.0 0.011045589781047905 3.2391758888703535E-5 30-31 3.2391758888703535E-5 0.0 0.0 0.03509647075591028 3.2391758888703535E-5 32-33 3.2391758888703535E-5 0.0 0.0 0.08940125453282176 3.2391758888703535E-5 34-35 3.2391758888703535E-5 0.0 0.0 0.16641266129071441 3.2391758888703535E-5 36-37 3.2391758888703535E-5 0.0 0.0 0.2886267675777928 3.2391758888703535E-5 38-39 3.2391758888703535E-5 0.0 0.0 0.47217466932063146 3.2391758888703535E-5 40-41 3.2391758888703535E-5 0.0 0.0 0.6803888954572177 3.2391758888703535E-5 42-43 3.2391758888703535E-5 0.0 0.0 0.885104811633824 3.2391758888703535E-5 44-45 3.2391758888703535E-5 0.0 0.0 1.1279944156607675 3.2391758888703535E-5 46-47 3.2391758888703535E-5 0.0 0.0 1.4186780599279931 3.2391758888703535E-5 48-49 3.2391758888703535E-5 0.0 0.0 1.7196946752807152 3.2391758888703535E-5 50-51 3.2391758888703535E-5 0.0 0.0 2.0348179016294674 3.2391758888703535E-5 52-53 3.2391758888703535E-5 0.0 0.0 2.3429930956965928 3.2391758888703535E-5 54-55 6.478351777740707E-5 0.0 0.0 2.671672273140268 3.2391758888703535E-5 56-57 6.478351777740707E-5 1.6195879444351767E-5 0.0 3.0493277900236624 3.2391758888703535E-5 58-59 6.478351777740707E-5 3.2391758888703535E-5 0.0 3.4374782367869967 3.2391758888703535E-5 60-61 6.478351777740707E-5 3.2391758888703535E-5 0.0 3.832576715831958 3.2391758888703535E-5 62-63 6.478351777740707E-5 3.2391758888703535E-5 0.0 4.214556532526995 3.2391758888703535E-5 64-65 6.478351777740707E-5 3.2391758888703535E-5 0.0 4.595580792334815 3.2391758888703535E-5 66-67 6.478351777740707E-5 3.2391758888703535E-5 0.0 4.999344066882504 3.2391758888703535E-5 68-69 6.478351777740707E-5 3.2391758888703535E-5 0.0 5.453719464693792 3.2391758888703535E-5 70-71 6.478351777740707E-5 3.2391758888703535E-5 0.0 5.9762633190863585 3.2391758888703535E-5 72-73 6.478351777740707E-5 3.2391758888703535E-5 0.0 6.507876865967761 3.2391758888703535E-5 74-75 6.478351777740707E-5 3.2391758888703535E-5 0.0 7.038178546614171 3.2391758888703535E-5 76-77 6.478351777740707E-5 3.2391758888703535E-5 0.0 7.595948438798201 3.2391758888703535E-5 78-79 6.478351777740707E-5 3.2391758888703535E-5 0.0 8.2229071279685 3.2391758888703535E-5 80-81 6.478351777740707E-5 3.2391758888703535E-5 0.0 8.892412392439116 3.2391758888703535E-5 82-83 6.478351777740707E-5 3.2391758888703535E-5 0.0 9.549430633858133 3.2391758888703535E-5 84-85 6.478351777740707E-5 3.2391758888703535E-5 0.0 10.193022491217786 3.2391758888703535E-5 86-87 6.478351777740707E-5 3.2391758888703535E-5 0.0 10.852437722794566 3.2391758888703535E-5 88-89 6.478351777740707E-5 3.2391758888703535E-5 0.0 11.541896310740622 3.2391758888703535E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGGCG 85 6.458395E-4 27.904993 8 CATTCCG 400 0.0 27.277132 9 GTACATG 16640 0.0 25.638046 1 TACATGG 17190 0.0 24.565317 2 ACATGGG 17015 0.0 23.670454 3 GAGTACT 12565 0.0 22.237741 12-13 CGATCGT 125 9.649739E-9 20.873274 24-25 GTACTTT 13535 0.0 20.591476 14-15 CATGGGG 4795 0.0 20.578112 4 AGTACTT 13145 0.0 20.5528 12-13 CATGGGA 12555 0.0 19.345684 4 GTGTAGC 1795 0.0 19.034725 1 CGCATAG 240 0.0 18.935368 66-67 GCACCGT 455 0.0 18.766874 6 GTATCAA 30285 0.0 18.740543 1 GGTATCA 24410 0.0 18.332533 1 ACTTTTT 15675 0.0 18.022377 16-17 ATGGGAT 4940 0.0 17.381308 5 TACACCG 275 5.995116E-7 17.250359 5 TACTTTT 15880 0.0 17.042881 14-15 >>END_MODULE