FastQCFastQC Report
Fri 27 May 2016
SRR939809_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939809_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789281
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126601.6039914808540938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92101.1668847976829546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69540.8810550361658268No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58530.7415609903190372No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35090.44458184094131237No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25660.32510601420786767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22130.280381765176154No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20460.2592232677588843No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.21247185729797122No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.20626367542104776No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15110.19144005747002651No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.17953048407347955No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG12750.16153942638933408No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11950.15140361924333667No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11560.14646241325966292No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11550.14633571567033793No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC10090.12783786762889263No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG9820.12441703271711849No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8340.10566578949702324No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7980.1011046762813244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGT1450.039.2617346
CGTCACT1900.034.956721
CATATAG1900.034.95453
GCGCAGA1703.6379788E-1233.487951
GTCAACG851.645429E-533.487951
GACATAT1950.031.6275081
GGGACCG902.4324921E-531.6275049
TGGGAGT3300.030.1898926
AACGGAG953.5205852E-529.9615
CATGGGT5400.028.1115574
GCCTATC856.451888E-427.9066267
GATAGTC1703.6852725E-927.9066267
GTACAGG1554.6389687E-827.5465371
CATGGGG21450.026.9811974
GTATCAA149400.026.9278371
GTACATG98050.026.6600041
TACATGG99200.026.3970932
GTTCGAC1007.2577677E-1026.10346216-17
ATGGGAT21250.025.8957045
ACATGGG100500.025.2531973
Produced by FastQC (version 0.11.5)